Abstract:
:The electron transfer pathway to Fe(III) reduction in Geobacter sulfurreducens has been hypothesized to consist of a series of c-type cytochromes. Previous genetic studies suggested that the inner membrane-associated, c-type cytochrome, MacA, was a component of the electron transfer chain leading to Fe(III) reduction in the dissimilatory Fe(III)-reducer, G. sulfurreducens. However, investigation of the expression of OmcB, an outer-membrane c-type cytochrome demonstrated previously to be critical for optimal Fe(III) reduction, revealed that both omcB transcript and protein levels were dramatically reduced in the MacA-deficient mutant. Expression of the omcB gene in trans enabled the MacA-deficient mutant to reduce Fe(III) at a rate that was proportional to the level of omcB expression. These results suggest that MacA is not directly involved in electron transfer to Fe(III) and further confirm the importance of OmcB in Fe(III) reduction by G. sulfurreducens.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Kim BC,Lovley DRdoi
10.1111/j.1574-6968.2008.01252.xsubject
Has Abstractpub_date
2008-09-01 00:00:00pages
39-44issue
1eissn
0378-1097issn
1574-6968pii
FML1252journal_volume
286pub_type
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