Androgen binding proteins in Cochliobolus lunatus.

Abstract:

:Testosterone and 4-androstene-3,17-dione (androstenedione) binding proteins were found in the cytosol of Cochliobolus lunatus, a filamentous fungus. The maximal androgen binding occurred by 120 min at 0 degrees C. Androgen binding proteins were degraded by protease treatment and at high temperature. The mean Kd determined for testosterone binding protein (TBP) by Scatchard analysis was 4.5 nM and the maximum capacity Bmax was 120 fmol/mg protein; for androstenedione binding protein (ABP) the Kd was 8 nM and Bmax was 390 fmol/mg protein. Steroid binding specificity for TBP, in order of decreasing affinity, was the following: androstenedione, progesterone, dihydrotestosterone, 17 beta-estradiol and estrone. The same order was found for ABP.

journal_name

FEMS Microbiol Lett

authors

Kastelic-Suhadolc T,Lenasi H

doi

10.1016/0378-1097(93)90498-q

subject

Has Abstract

pub_date

1993-03-15 00:00:00

pages

121-5

issue

1

eissn

0378-1097

issn

1574-6968

journal_volume

108

pub_type

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