Properties of yeast expressed Aspergillus nidulans chitin synthase B which is essential for hyphal growth.

abstract：:Photorhabdus (Enterobacteriaceae) bacteria are pathogenic to insects and mutualistic with entomopathogenic Heterorhabditis nematodes. Photorhabdus luminescens subsp. akhurstii LN2, associated with Heterorhabditis indica LN2, shows nematicidal activity against H. bacteriophora H06 infective juveniles (IJs). In the pres...

journal_title：FEMS microbiology letters

authors： Qiu X,Wu C,Cao L,Ehlers RU,Han R

更新日期：2016-03-01 00:00:00

abstract：:A thorough understanding of the mechanisms of Rcs and EnvZ/OmpR phosphorelay systems that allow Yersinia enterocolitica to thrive in various environments is crucial to prevent and control Y. enterocolitica infections. In this study, we showed that RcsB and OmpR have the ability to function differently in modulating a ...

journal_title：FEMS microbiology letters

authors： Meng J,Bai J,Xu J,Huang C,Chen J

更新日期：2019-09-01 00:00:00

abstract：:Streptococcus intermedius is well known to produce severe infections in various areas of the body. In this study, we evaluated the ability of S. intermedius to utilise human transferrin as a source of iron and investigated the mechanism by which iron can be obtained from this plasma protein. Adding either ferrous sulf...

journal_title：FEMS microbiology letters

authors： Brochu V,Greinier D,Mayrand D

更新日期：1998-09-01 00:00:00

abstract：:Coagulase-negative staphylococci (CoNS) are common bacterial colonizers of the human skin. They are often involved in nosocomial infections due to biofilm formation in indwelling medical devices. While biofilm formation has been extensively studied in Staphylococcus epidermidis, little is known regarding other CoNS sp...

journal_title：FEMS microbiology letters

authors： Oliveira F,Lima CA,Brás S,França Â,Cerca N

更新日期：2015-10-01 00:00:00

abstract：:Molecular microbial ecology studies are heavily reliant on 'Universal' 16S rRNA gene primers for elucidating microbial community structure and composition, and yet primer design and optimization is often overlooked. Primers that exhibit minor biases due to primer-template mismatches can substantially alter the pool of...

journal_title：FEMS microbiology letters

authors： Thomas MC,Thomas DK,Selinger LB,Inglis GD

更新日期：2011-07-01 00:00:00

abstract：:Helicobacter pullorum and Campylobacter lari are rarely isolated from humans with acute enteritis. Hitherto the two species could only be identified by genotypic techniques. Gas liquid chromatography of whole cell fatty acid extracts is described as the first phenotypic method for discrimination of the two species. Ch...

journal_title：FEMS microbiology letters

authors： Steinbrueckner B,Haerter G,Pelz K,Burnens A,Kist M

更新日期：1998-11-15 00:00:00

abstract：:Synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) protocol to target a specific sequence in the gene coding for the A subunit of Escherichia coli verotoxin (VTe-variant, VTev). This PCR protocol permits the VTe-variant target sequence to be distinguished from closely related sequences in...

journal_title：FEMS microbiology letters

authors： Johnson WM,Tyler SD,Wang G,Lior H

更新日期：1991-11-15 00:00:00

abstract：:Morphologically similar species such as Chinese black truffles and Tuber melanosporum were typed by restriction length polymorphism of internal transcribed spacers. This analysis together with sequence comparison revealed the presence of high genetic variability among fruit bodies collected in China. Selection of prim...

journal_title：FEMS microbiology letters

authors： Paolocci F,Rubini A,Granetti B,Arcioni S

更新日期：1997-08-15 00:00:00

abstract：:Mutants in Aspergillus niger unable to grow on acetate as a sole carbon source were previously isolated by resistance to 1.2% propionate medium containing 0.1% glucose. AcuA mutants lacked acetyl-CoA synthetase (ACS) activity and acuB mutants lacked both ACS and isocitrate lyase activity. An acuA mutant was transforme...

journal_title：FEMS microbiology letters

authors： Papadopoulou S,Sealy-Lewis HM

更新日期：1999-09-01 00:00:00

abstract：:Mini-Tn10luxABcam/Ptac-ATS was constructed in order to develop a luciferase-transducing bacteriophage for detecting Escherichia coli O157:H7. The transposon was designed to deliver a 3.6-kb insertion that confers n-decanal-dependent bioluminescence and resistance to chloramphenicol and was constructed using mini-Tn10c...

journal_title：FEMS microbiology letters

authors： Waddell TE,Poppe C

更新日期：2000-01-15 00:00:00

abstract：:Two thermostable phytases were identified from Thai isolates of Aspergillus japonicus BCC18313 (TR86) and Aspergillus niger BCC18081 (TR170). Both genes of 1404 bp length, coding for putative phytases of 468 amino acid residues, were cloned and transferred into Pichia pastoris. The recombinant phytases, r-PhyA86 and r...

journal_title：FEMS microbiology letters

authors： Promdonkoy P,Tang K,Sornlake W,Harnpicharnchai P,Kobayashi RS,Ruanglek V,Upathanpreecha T,Vesaratchavest M,Eurwilaichitr L,Tanapongpipat S

更新日期：2009-01-01 00:00:00

abstract：:Vf33 is a filamentous bacteriophage isolated from Vibrio parahaemolyticus. We performed Southern blot hybridization analysis to examine the distribution of Vf33-related genetic elements in the pandemic strains (O3:K6 strains isolated between 1995 and 1997, O4:K68 and O1:K untypeable strains isolated between 1997 and 1...

journal_title：FEMS microbiology letters

authors： Chang B,Yoshida S,Miyamoto H,Ogawa M,Horikawa K,Ogata K,Nishibuchi M,Taniguchi H

更新日期：2000-11-15 00:00:00

abstract：:Aceticlastic methanogens metabolize acetate to methane and carbon dioxide. The central metabolism and the electron transport chains of these organisms have already been investigated. However, no particular attention has been paid to the mechanism by which acetate enters the archaeal cell. In our study we investigated ...

journal_title：FEMS microbiology letters

authors： Welte C,Kröninger L,Deppenmeier U

更新日期：2014-10-01 00:00:00

abstract：:The diversity of temperate bacteriophages was examined in 32 Mannheimia haemolytica, six Mannheimia glucosida and four Pasteurella trehalosi isolates. Phage particles were induced and identified by electron microscopy in 24 (75%) M. haemolytica isolates, but in only one (17%) M. glucosida and one (25%) P. trehalosi is...

journal_title：FEMS microbiology letters

authors： Davies RL,Lee I

更新日期：2006-07-01 00:00:00

abstract：:Haloarchaea thrive under saline and hypersaline conditions and often dominate microbial communities in saltmarshes, salted lakes/soils and some oceanic areas. Some of the predominant species show denitrifying capabilities, although it remains unclear whether they are complete or partial denitrifiers. As complete denit...

journal_title：FEMS microbiology letters

authors： Torregrosa-Crespo J,Bergaust L,Pire C,Martínez-Espinosa RM

更新日期：2018-02-01 00:00:00

abstract：:The gene (open reading frame Tm1155, g6pd) encoding glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49) of the hyperthermophilic bacterium Thermotoga maritima was cloned and functionally expressed in Escherichia coli. The purified recombinant enzyme is a homodimer with an apparent molecular mass of 95 kDa composed o...

journal_title：FEMS microbiology letters

authors： Hansen T,Schlichting B,Schönheit P

更新日期：2002-11-05 00:00:00

abstract：:Currently, interest in phage science is on the rise again as this subject remains largely unexplored, and its potential diversity mainly untapped. Here, we present phage vB_BsuP-Goe1, a new isolate that infects Bacillus subtilis The phage forms round plaques with a matt outline on agar plates containing a B. subtilis ...

journal_title：FEMS microbiology letters

authors： Willms IM,Hertel R

更新日期：2016-10-01 00:00:00

abstract：:Aspergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter (PcatR) from A. niger was characterized. For this, constructs were developed and checked for the expression of the ...

journal_title：FEMS microbiology letters

authors： Sharma R,Katoch M,Govindappa N,Srivastava PS,Sastry KN,Qazi GN

更新日期：2012-02-01 00:00:00

abstract：:TraJ is an activator of the transfer (tra) operon in the F plasmid that counteracts H-NS silencing at the main transfer promoter (P(Y)). TraJ contains 226 aa (26 670 kDa), not 229 aa as reported previously, and forms homodimers. TraJ binds DNA containing P(Y)in vivo as demonstrated using a chromatin-immunoprecipitatio...

journal_title：FEMS microbiology letters

authors： Rodriguez-Maillard JM,Arutyunov D,Frost LS

更新日期：2010-09-01 00:00:00

abstract：:An Acremonium chrysogenum strain improvement program based on the transformation with cephalosporin biosynthetic genes was carried out to enhance cephalosporin C production. Best results were obtained with cefEF and cefG genes, selecting transformants with increased cephalosporin C production and lower accumulation of...

journal_title：FEMS microbiology letters

authors： Rodríguez-Sáiz M,Lembo M,Bertetti L,Muraca R,Velasco J,Malcangi A,de la Fuente JL,Barredo JL

更新日期：2004-06-01 00:00:00

abstract：:The core metabolism of microorganisms has a major influence on the hydrogen isotopic composition of their fatty acids. Heterotrophic microorganisms produce fatty acids with a deuterium to hydrogen (D/H) ratio either slightly depleted or enriched in D compared to the growth water, while photo- and chemoautotrophic micr...

journal_title：FEMS microbiology letters

authors： Heinzelmann SM,Chivall D,M'Boule D,Sinke-Schoen D,Villanueva L,Damsté JS,Schouten S,van der Meer MT

更新日期：2015-05-01 00:00:00

abstract：:Beauveria bassiana is an insect pathogenic fungus widely used as a biocontrol agent to infect and control insect pests. The conidium initiates pathogenesis and mediates disease transmission, however, little is known about genetic elements that control conidiation. Here, the cloning and characterization of a regulatory...

journal_title：FEMS microbiology letters

authors： Fang W,Scully LR,Zhang L,Pei Y,Bidochka MJ

更新日期：2008-02-01 00:00:00

abstract：:Bacillus subtilis possesses two isogenes encoding glutamate racemases, the poly-gamma-glutamate synthesis-linking Glr enzyme and the YrpC isozyme, and produces abundant amounts of the Glr enzyme. The YrpC isozyme, but not the Glr enzyme, was found to influence the activity of DNA gyrase, as did the MurI-type glutamate...

journal_title：FEMS microbiology letters

authors： Ashiuchi M,Kuwana E,Komatsu K,Soda K,Misono H

更新日期：2003-06-27 00:00:00

abstract：:An enzyme linked immune sorbent assay (ELISA) was developed to measure bacterial surface lipoteichoic acid (LTA). Numerous strains of oral streptococci belonging to the 'viridans' group were examined on three separate occasions. The results show that, under these cultural conditions, oral streptococci do not normally ...

journal_title：FEMS microbiology letters

authors： Hogg SD,Manning JE

更新日期：1989-04-01 00:00:00

abstract：:Transcription of the sigA and sigB genes of Brevibacterium lactofermentum, encoding the principal sigma factors of RNA polymerase, has been investigated by Northern blot and primer extension analysis. Northern hybridizations revealed that sigA is transcribed as a monocistronic mRNA of 1.7 kb and sigB gives two transcr...

journal_title：FEMS microbiology letters

authors： Oguiza JA,Marcos AT,Martín JF

更新日期：1997-08-01 00:00:00

abstract：:To clarify the cause of the supersusceptibility of Branhamella catarrhalis to macrolide antibiotics, which are well-known to be inactive to most Gram-negative bacteria, we determined its cell surface hydrophobicity by the partition experiment between water and hydrocarbons. Its cell surface was found to be markedly mo...

journal_title：FEMS microbiology letters

authors： Gotoh N,Tanaka S,Nishino T

更新日期：1989-05-01 00:00:00

abstract：:The study of exopolysaccharide production by heterofermentative sourdough lactic acid bacteria has shown that Weissella strains isolated from sourdoughs produce linear dextrans containing α-(1→6) glucose residues with few α-(1→3) linkages from sucrose. In this study, several dextran-producing strains, Weissella cibari...

journal_title：FEMS microbiology letters

authors： Bounaix MS,Robert H,Gabriel V,Morel S,Remaud-Siméon M,Gabriel B,Fontagné-Faucher C

更新日期：2010-10-01 00:00:00

abstract：:parD and chpA are homologous conditional killer systems of plasmid and chromosomal origin, respectively, encoding a killer protein (Kid and ChpAK) and an antidote (Kis and ChpAI). Here it is shown that these systems can functionally interact. A multicopy chpA recombinant partially complements two mutations in the anti...

journal_title：FEMS microbiology letters

authors： Santos-Sierra S,Giraldo R,Díaz-Orejas R

更新日期：1997-07-01 00:00:00

abstract：:Pseudomonas syringae pv. tomato DC3000, a plant pathogenic gram-negative bacterium, employs the type III secretion system (T3SS) to cause disease in tomato and Arabidopsis and to induce the hypersensitive response in nonhost plants. The expression of T3SS is regulated by the HrpL extracytoplasmic sigma factor. Express...

journal_title：FEMS microbiology letters

authors： Jovanovic M,Lawton E,Schumacher J,Buck M

更新日期：2014-07-01 00:00:00

abstract：:The synthesis of inducible biodegradative threonine dehydratase of Escherichia coli increased several-fold in the presence of the DNA gyrase inhibitors, nalidixic acid and coumermycin. Temperature-sensitive gyrB mutants expressed higher levels of dehydratase as compared to an isogenic gyrB+ strain. Immunoblotting expe...

journal_title：FEMS microbiology letters

authors： Sumantran VN,Tranguch AJ,Datta P

更新日期：1989-11-01 00:00:00