Development of TaqMan assays for the quantitative detection of Fusarium avenaceum/Fusarium tricinctum and Fusarium poae esyn1 genotypes from cereal grain.

abstract：:Agrobacterium tumefaciens strain SCUEC1 is a nicotine-degrading bacterium, which has been recently isolated from the tobacco waste-contaminated field soil. However, the mechanism for nicotine degradation in this strain remains unclear. Here, we analyze the function and biological properties of the agnH gene in the str...

journal_title：FEMS microbiology letters

authors： Xia Z,Yu M,Yao J,Feng Z,Li D,Liu T,Cheng G,He D,Li X

更新日期：2020-02-01 00:00:00

abstract：:In several bacterial species that show natural transformation, dprA has been described as a competence gene. The DprA protein has been suggested to be involved in the protection of incoming DNA. However, members of the dprA gene family (also called smf) can be detected in virtually all bacterial species, which suggest...

journal_title：FEMS microbiology letters

authors： Smeets LC,Becker SC,Barcak GJ,Vandenbroucke-Grauls CM,Bitter W,Goosen N

更新日期：2006-10-01 00:00:00

abstract：:The need for improved rapid diagnostic tests for tuberculosis disease has prompted interest in the volatile organic compounds (VOCs) emitted by Mycobacterium tuberculosis complex bacteria. We have investigated VOCs emitted by Mycobacterium bovis BCG grown on Lowenstein-Jensen media using selected ion flow tube mass sp...

journal_title：FEMS microbiology letters

authors： McNerney R,Mallard K,Okolo PI,Turner C

更新日期：2012-03-01 00:00:00

abstract：:As shown by both bioassay and high-performance liquid chromatographic (HPLC) analysis, penicillin G production by Aspergillus nidulans is subject to regulation by the pH of the growth medium. Penicillin titres were highest at alkaline pH and in strains carrying mutations in the regulatory gene pacC which mimics the ef...

journal_title：FEMS microbiology letters

authors： Shah AJ,Tilburn J,Adlard MW,Arst HN Jr

更新日期：1991-01-15 00:00:00

abstract：:In haloarchaea, a cluster of three genes is localized directly adjacent to the major replication origin, and, hence, the encoded proteins were annotated as 'origin-associated proteins' (Oap). However, prior to this study, no experimental data were available for these conserved hypothetical proteins. Bioinformatic anal...

journal_title：FEMS microbiology letters

authors： Wolters M,Borst A,Pfeiffer F,Soppa J

更新日期：2019-11-01 00:00:00

abstract：:The xylanase gene of Streptomyces flavogriseus was cloned in pUC8 plasmid and expressed in Escherichia coli lysogenic for lambda cI857. lambda-Induced lysis of E. coli at 42 degrees C allowed efficient release of cloned enzyme activity in extracellular environment. The xylanase gene was located in the 0.8-kb HindIII f...

journal_title：FEMS microbiology letters

authors： Srivastava R,Ali SS,Srivastava BS

更新日期：1991-03-01 00:00:00

abstract：:Cells of the pel1 mutant of Saccharomyces cerevisiae were found to contain an extremely low content of cardiolipin, a decreased level of phosphatidylcholine and an increased level of phosphatidylinositol. Disruption of the PEL1 gene in cells containing a null mutation in the CHO1 gene was lethal. Despite its putative ...

journal_title：FEMS microbiology letters

authors： Janitor M,Obernauerová M,Kohlwein SD,Subík J

更新日期：1996-06-15 00:00:00

abstract：:The effect of Listeria monocytogenes infection on the cellular level of transcription factor NF-kappa B in the human epithelia-like cell line Caco-2 was investigated. Infection with L. monocytogenes or treatment with lipoteichoic acid induced the formation of three NF-kappa B-like DNA-protein complexes C1, C2, and C3,...

journal_title：FEMS microbiology letters

authors： Hauf N,Goebel W,Fiedler F,Kuhn M

更新日期：1999-09-01 00:00:00

abstract：:A cold shock domain (CSD)-containing protein, CspD, of molecular mass ~7.28 kDa in a psychrotolerant Antarctic Janthinobacterium sp. Ant5-2 (ATCC BAA-2154) exhibited constitutive expression at 37, 22, 15, 4 and -1°C. The cspD gene encoding the CspD protein of Ant5-2 was cloned, sequenced and analyzed. The deduced prot...

journal_title：FEMS microbiology letters

authors： Mojib N,Andersen DT,Bej AK

更新日期：2011-06-01 00:00:00

abstract：:The gene encoding the second enzyme of the 2C-methyl-D-erythritol 4-phosphate (MEP) pathway for isopentenyl diphosphate biosynthesis, 1-deoxy-D-xylulose 5-phosphate (DXP) reductoisomerase, was cloned and sequenced from Zymomonas mobilis. The deduced amino acid sequence showed the highest identity (48.2%) to the DXP re...

journal_title：FEMS microbiology letters

authors： Grolle S,Bringer-Meyer S,Sahm H

更新日期：2000-10-01 00:00:00

abstract：:Enterobacter cloacae CHE, a clinical strain with overproduced cephalosporinase was found to be highly resistant to the new cephalosporins, cefepime and cefpirome (MICs> or =128 microg ml(-1)). The strain was isolated from a child previously treated with cefepime. The catalytic efficiency of the purified enzyme with th...

journal_title：FEMS microbiology letters

authors： Barnaud G,Labia R,Raskine L,Sanson-Le Pors MJ,Philippon A,Arlet G

更新日期：2001-02-20 00:00:00

abstract：:The enterotoxigenic Escherichia coli (ETEC) strain Ec2173, causing post weaning diarrhoea in swine, harbours six plasmids ranging from 13 to 200 kb in size. The heat stable toxin genes sta, stb and a tetracycline resistance gene were located on a self conjugative 120-kb plasmid, called pTC. In the cloned ColE1 type or...

journal_title：FEMS microbiology letters

authors： Olasz F,Fekete PZ,Blum-Oehler G,Boldogkoi Z,Nagy B

更新日期：2005-03-15 00:00:00

abstract：:Tachyzoites of Toxoplasma gondii multiply within the parasitophorous vacuole (PV) until the lysis of the host cell. This study was undertaken to evaluate the effect of hydroxyurea (a specific drug that arrests cell division at G1/S phase) on the multiplication of T. gondii tachyzoites in infected Vero cells. Infected ...

journal_title：FEMS microbiology letters

authors： de Melo EJ,Mayerhoffer RO,de Souza W

更新日期：2000-04-01 00:00:00

abstract：:Ammonia-induced injury was investigated in pure cultures of Escherichia coli and Enterobacter aerogenes, and in natural coliform populations obtained from the oligotrophic Luxapallila and the eutrophic Sunflower Rivers in northern Mississippi. Pure cultures were affected by ammonia exposure as indicated by changes in ...

journal_title：FEMS microbiology letters

authors： Naundorf G,Aumen NG

更新日期：1989-11-01 00:00:00

abstract：:Autophagy, a eukaryotic cellular activity leading to the degradation of cellular components, serves as a defense mechanism against facultative intracellular bacteria as well as a growth niche for the obligate intracellular bacterium Coxiella burnetii. We here demonstrate that the obligate intracellular bacterial patho...

journal_title：FEMS microbiology letters

authors： Pachikara N,Zhang H,Pan Z,Jin S,Fan H

更新日期：2009-03-01 00:00:00

abstract：:We analyzed gel electrophoretic banding patterns of penicillin-binding proteins (PBPs) of 16 type strains of coagulase-negative staphylococci (CNS). S. epidermidis, S. haemolyticus, S. saprophyticus, S. hominis, S. xylosus, S. simulans, S. warneri, S. capitis, S. saccharolyticus, S. auricularis, S. caseolyticus, S. ga...

journal_title：FEMS microbiology letters

authors： Kanda K,Hiramatsu K,Yokota T

更新日期：1990-10-01 00:00:00

abstract：:Hemerythrins are oxygen-binding proteins found in the body fluids and tissues of certain invertebrates. Oxygen is bound at a nonheme iron centre consisting of two oxo-bridged iron atoms bound to a characteristic set of conserved histidine: aspartate and glutamate residues with the motifs H-HxxxE-HxxxH-HxxxxD. It has r...

journal_title：FEMS microbiology letters

authors： French CE,Bell JM,Ward FB

更新日期：2008-02-01 00:00:00

abstract：:The distribution of the antiseptic-resistance genes qacE and qacE delta 1 was studied in a large number of Gram-negative bacteria by a method that included the polymerase chain reaction (PCR). A total of 117 strains of Gram-negative bacteria, isolated from clinical or environmental sources, was used in this analysis. ...

journal_title：FEMS microbiology letters

authors： Kazama H,Hamashima H,Sasatsu M,Arai T

更新日期：1998-02-15 00:00:00

abstract：:Most Moraxella catarrhalis isolates express a highly-conserved outer membrane protein of 453 residues designated OMPCD, which has been previously shown to mediate binding to A549 human lung cells. Here, it is reported that two distinct domains of the M. catarrhalis strain O35E OMPCD protein specify adherence. Truncate...

journal_title：FEMS microbiology letters

authors： Akimana C,Lafontaine ER

更新日期：2007-06-01 00:00:00

abstract：:The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid diff...

journal_title：FEMS microbiology letters

authors： Steinthorsdottir V,Fridriksdottir V,Gunnarsson E,Andrésson OS

更新日期：1995-08-01 00:00:00

abstract：:The number and incidence of Collinsella aerofaciens in the human intestine are the highest among Gram-positive non-spore-forming bacilli. Identification of this species is very difficult and requires considerable time. A PCR-based identification system using C. aerofaciens-specific primers is described. Using this PCR...

journal_title：FEMS microbiology letters

authors： Kageyama A,Sakamoto M,Benno Y

更新日期：2000-02-01 00:00:00

abstract：:The enzymic interconversion of Escherichia coli isocitrate dehydrogenase (ICDH) between the catalytically active and inactive forms is mediated through the activities of ICDH-kinase/phosphatase in response to changes in the metabolic environment. In this study, the use of mutant strains devoid of isocitrate lyase (ace...

journal_title：FEMS microbiology letters

authors： el-Mansi EM

更新日期：1998-09-15 00:00:00

abstract：:We have isolated the abc1 gene from the fission yeast Schizosaccharomyces pombe. Sequence analysis suggests that the Abc1 protein is a member of the ABC superfamily of transporters and is composed of two structurally homologous halves, each consisting of a hydrophobic region of six transmembrane domains and a hydrophi...

journal_title：FEMS microbiology letters

authors： Christensen PU,Davis K,Nielsen O,Davey J

更新日期：1997-02-01 00:00:00

abstract：:A gene fusion between the Saccharomyces cerevisiae actin gene promoter and the cDNA of the Fusarium solani f. sp. pisi pelA gene has been constructed. This expression cassette has been introduced into the industrial wine yeast strain T73. The resulting recombinant strain is able to secrete active PELA enzyme into the ...

journal_title：FEMS microbiology letters

authors： González-Candelas L,Cortell A,Ramon D

更新日期：1995-03-01 00:00:00

abstract：:Pyruvate, the key regulator in connection of a variety of metabolic pathways, influences transcription of the Escherichia coli genome through controlling the activity of two pyruvate-sensing two-component systems (TCSs), BtsSR and PyrSR. Previously, we identified the whole set of regulatory targets of PyrSR with low-a...

journal_title：FEMS microbiology letters

authors： Ogasawara H,Ishizuka T,Yamaji K,Kato Y,Shimada T,Ishihama A

更新日期：2019-12-01 00:00:00

abstract：:The cea-kil operon of the ColE1 plasmid is negatively regulated by the LexA-repressor and therefore, it is under the control of SOS regulation. We constructed a gene fusion between the cea and lacZ genes. Expression of the translational fusion can be easily detected by monitoring the levels of beta-galactosidase. Sinc...

journal_title：FEMS microbiology letters

authors： Schumann W,Songür N,Miltenburger HG

更新日期：1991-07-01 00:00:00

abstract：:The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhim...

journal_title：FEMS microbiology letters

authors： Liao H,Jiang L,Zhang R

更新日期：2018-02-01 00:00:00

abstract：:Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera in poultry, hemorrhagic septicemia in cattle, atropic rhinitis in swine, and snuffles in rabbits. The differentially expressed gene profile of P. multocida in infected rabbit livers was identified and compared with t...

journal_title：FEMS microbiology letters

authors： Guo D,Lu Y,Zhang A,Liu J,Yuan D,Jiang Q,Lin H,Si C,Qu L

更新日期：2012-06-01 00:00:00

abstract：:Divergicin 750, a bacteriocin produced by Carnobacterium divergens 750, preferentially inhibited the growth of strains of Carnobacterium and Enterococcus. Selected strains of Listeria monocytogenes and Clostridium perfringens were also inhibited. The bacteriocin was purified to homogeneity by ammonium sulfate precipit...

journal_title：FEMS microbiology letters

authors： Holck A,Axelsson L,Schillinger U

更新日期：1996-02-15 00:00:00

abstract：:In this report we describe the development of a highly stringent and dually regulated promoter system for Shigella flexneri. Dual regulation was provided by utilizing a promoter susceptible to control by the bacteriophage P1 temperature-sensitive C1 repressor that in turn was under the transcriptional control of LacI....

journal_title：FEMS microbiology letters

authors： Schofield DA,Westwater C,Dolan JW,Norris JS,Schmidt MG

更新日期：2002-10-08 00:00:00