Activation of FNR-dependent transcription by iron: an in vitro switch for FNR.

Abstract:

:FNR is an iron-binding transcriptional regulator of Escherichia coli which controls the expression of target genes in response to anaerobiosis. The mechanism used by FNR to sense and respond to anaerobiosis is unknown but it is thought to involve iron. In vitro transcription analyses have shown that iron-deficient FNR is unable to activate transcription from the FF-melR promoter, but activity could be restored by preincubation with Fe2+ and beta-mercaptoethanol. The reactivation of FNR was prevented and reversed by chelating agents, and this reactivation thus provides an artificial in vitro switch for FNR-dependent transcriptional activation.

journal_name

FEMS Microbiol Lett

authors

Green J,Guest JR

doi

10.1111/j.1574-6968.1993.tb06517.x

subject

Has Abstract

pub_date

1993-10-15 00:00:00

pages

219-22

issue

2

eissn

0378-1097

issn

1574-6968

pii

0378-1097(93)90272-4

journal_volume

113

pub_type

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