Abstract:
:We recently reported behavioral improvements following intrastriatal transplantation of cryopreserved cultured human neuroteratocarcinoma-derived cells (hNT neurons) in rats with cerebral ischemia induced by occlusion of the middle cerebral artery. In the present study, the viability and survival of hNT neurons were evaluated immediately prior to the transplantation surgery and at 3 months post-transplantation in ischemic rats. Cryopreserved hNT neurons were routinely thawed, and trypan blue exclusion viability counts revealed 52-95% viable hNT neurons before transplantation. Monthly behavioral tests, starting at 1 month and extending to 3 months post-transplantation, revealed that ischemic animals that were intrastriatally transplanted with hNT neurons (approximately 40000) and treated with an immunosuppressive drug displayed normalization of asymmetrical motor behavior compared with ischemic animals that received medium alone. Within-subject comparisons of cell viability and subsequent behavioral changes revealed that a high cell viability just prior to transplantation surgery correlated highly with a robust and sustained functional improvement in the transplant recipient. Furthermore, histological analysis of grafted brains revealed a positive correlation between number of surviving hNT neurons and degree of functional recovery. In concert with similar reports on fetal tissue transplantation, we conclude that high cell viability is an important criterion for successful transplantation of cryopreserved neurons derived from cell lines to enhance graft-induced functional effects.
journal_name
Neuroreportjournal_title
Neuroreportauthors
Borlongan CV,Saporta S,Poulos SG,Othberg A,Sanberg PRdoi
10.1097/00001756-199808240-00028subject
Has Abstractpub_date
1998-08-24 00:00:00pages
2837-42issue
12eissn
0959-4965issn
1473-558Xjournal_volume
9pub_type
杂志文章相关文献
NEUROREPORT文献大全abstract::The role of the medial septum in suppressing paradoxical sleep and promoting slow wave sleep was suggested on the basis of neurotoxic lesion studies. However, these conclusions need to be substantiated with further experiments, including chemical stimulation studies. In this report, the medial septum was stimulated in...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0b013e3283533692
更新日期:2012-05-09 00:00:00
abstract::Voltage-gated sodium channels consist of a pore-containing alpha-subunit and one or more auxiliary beta-subunits, which may modulate channel function. We previously demonstrated that sodium channel SNS/PN3 alpha-subunits were decreased in human sensory cell bodies after spinal root avulsion injury, and accumulated at ...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200103050-00012
更新日期:2001-03-05 00:00:00
abstract::Diabetic peripheral neuropathy is the most common complication of diabetes mellitus and leads to sensory symptoms, including diabetic neuropathic pain (DNP). DNP is a major public health problem because it has a considerable impact on life quality of diabetes mellitus patients. Therefore, development of novel effectiv...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000001138
更新日期:2018-12-05 00:00:00
abstract::We screened proteins for interaction with presenilin (PS) 1, and cloned the full-length cDNA of human delta-catenin, which encoded 1225 amino acids. Yeast two-hybrid assay, GST binding assay and immunoprecipitation demonstrated that delta-catenin interacted with a hydrophilic loop region in the endoproteolytic C-termi...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199902250-00022
更新日期:1999-02-25 00:00:00
abstract::The aim of this study was to show that Cl(-)-dependent excitotoxicity, with its characteristic cell swelling, involves actual water influx into the intracellular compartment. Taking advantage of the Ca2+ omission paradigm of Cl(-)-dependent excitotoxicity, in the chick embryonic neural retina ex vivo, which is associa...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200011270-00037
更新日期:2000-11-27 00:00:00
abstract::We examined the effect of stimulus interruption on dual component field EPSPs in the hippocampal CA1 region. Resuming test stimulation at 0.1 Hz after 10-60 min silent periods led to an increase of the response followed by a decline, involving AMPA and NMDA components to a similar extent. Similar changes were seen whe...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199904060-00005
更新日期:1999-04-06 00:00:00
abstract::In isolated neurons of Helix pomatia, a two-microelectrode voltage clamp technique was used to study the effect of Fe3+ on voltage-gated potassium currents: a low-threshold fast-inactivating current (I(A)) and a high threshold slow-inactivating current with calcium-dependent (I(C)) and calcium-independent (I(DR)) comp...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0b013e3282c48c1c
更新日期:2007-08-27 00:00:00
abstract::Interleukin-6 (IL-6) is a neurotrophic cytokine, however, its direct effect on nerve regeneration has not been well characterized. We therefore examined the effect of IL-6 on neurite regeneration using the rat dorsal root ganglion. IL-6 significantly enhanced neurite regeneration from transected nerve terminals. We al...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200104170-00043
更新日期:2001-04-17 00:00:00
abstract::The tetrodotoxin-resistant voltage-gated sodium channel Nav 1.8 is expressed only in nociceptive sensory neurons. This channel has been proposed to contribute significantly to the sensitization of primary sensory neurons after injury. We have studied the nociceptive behaviours of mice carrying a null mutation in the N...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200110080-00019
更新日期:2001-10-08 00:00:00
abstract::Somatostatin sst2(a) receptor was observed, by immunofluorescence, in ependymal cells and in tanycytes of the wall of the ventricle and the hypothalamic recess of the male rat median eminence. Strong immunoreactivity for the receptor protein was observed in lateral tanycytes (alpha-type) while a moderate signal was se...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200107030-00007
更新日期:2001-07-03 00:00:00
abstract::Increased oxidative stress in the brain due to chronic ethanol consumption is known to result in a number of neurodegenerative changes. This study was designed to test whether dietary supplementation of grape polyphenols (GP) can offer protection to the neurodegenerative changes resulting from chronic ethanol consumpt...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199901180-00018
更新日期:1999-01-18 00:00:00
abstract::This study compared the visuospatial asymmetries in children with dyslexia and healthy children by using the manual line bisection task, and investigated the processing of spatial context with a 'local' cueing paradigm consisting of geometric symbols placed on the extremities of the lines. The performance between heal...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0b013e328342d2df
更新日期:2011-01-26 00:00:00
abstract::Cultured cerebellar granule neurons were used to examine the hypothesis that NMDA or KCl-dependent Ca2+ influx activates a signaling cascade mediating transcription, translation and assembly of neurofilaments. The data indicate that 25 mM KCl and 140 microM NMDA induce neurofilament protein, mRNA, and assembly in neur...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200311140-00013
更新日期:2003-11-14 00:00:00
abstract::The primate pulvinar connects with the entire array of known visual areas and is postulated to play a role in selective visual attention. Recently, five separate neurochemical subdivisions of a region termed the inferior pulvinar (PI) complex were identified in monkeys. In the present study, similar histochemical proc...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199912160-00002
更新日期:1999-12-16 00:00:00
abstract::We obtained visual response latencies and response durations following visual stimulation of neurones recorded in the lateral geniculate nucleus (LGN), areas 17, 18, 19, the posteromedial lateral suprasylvian sulcus (PMLS), and area 7 of the cat. We found an increasing lengthening of the mean response latencies, consi...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199404000-00010
更新日期:1994-04-14 00:00:00
abstract::N- and C-terminal substance P (SP) fragments increase striatal dopamine outflow at nanomolar concentrations. This contrasts with their low affinity for NK1 receptors. To explore this discrepancy, we investigated the interaction of SP and SP fragments with NK1 sites in fresh striatal slices, the same model used in the ...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199907130-00038
更新日期:1999-07-13 00:00:00
abstract::DJ-1, originally identified as an oncogene product, is involved in multiple processes, including cellular transformation, the oxidative stress response, and transcriptional regulation. We had previously found that DJ-1 was closely associated with the proliferation and differentiation of cultured neuronal stem cells. I...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000000956
更新日期:2018-03-07 00:00:00
abstract::The event-related potential technique was used to investigate the time course and scalp-potential topography for the numerical distance effect in a number-matching task. Twenty undergraduates judged whether a number matched or did not match another number presented 1.5 s earlier. Compared with number pairs with a 'sma...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/01.wnr.0000221840.12632.9f
更新日期:2006-07-17 00:00:00
abstract::The present study examined cerebral representations of Japanese long and short vowel categories with near-infrared spectroscopy (NIRS) by measuring the hemodynamic changes. Results showed that NIRS could capture phoneme-specific information. The left side of the auditory area showed large hemodynamic changes only for ...
journal_title:Neuroreport
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00001756-200204160-00009
更新日期:2002-04-16 00:00:00
abstract::Latest reports suggest the involvement of insulin in modulating memory. A few published in-vitro studies favor the antidementia effect of insulin. Thus, the present study aimed to evaluate the prophylactic role of insulin and its combination with glucose and its possible mechanism(s) in an aluminum chloride (AlCl3)-in...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000000799
更新日期:2017-06-14 00:00:00
abstract::Net-like structures surrounding several types of neurones contain glycoconjugates which are detectable by lectins specific for N-acetylgalactosamine. Wisteria floribunda agglutinin (WFA) was introduced as a further marker for the visualization of such perineuronal nets, which were also revealed in regions of the rat b...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199210000-00012
更新日期:1992-10-01 00:00:00
abstract::To determine whether physical match between studied and tested items influences blood flow increases in the hippocampal formation associated with recognition memory, positron emission tomography (PET) was used to measure changes in regional cerebral blood flow while healthy volunteers made old/new judgements about lin...
journal_title:Neuroreport
pub_type: 临床试验,杂志文章
doi:10.1097/00001756-199712220-00028
更新日期:1997-12-22 00:00:00
abstract::While dopamine mechanisms play a crucial role in cocaine-taking behavior, the contribution of endogenous opioid systems is less clear. We assessed the effects of opioid receptor blockade by naloxone (1 mg/kg, s.c.) on the daily performance and subsequent initiation of cocaine self-administration in trained rats. Nalox...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200302100-00019
更新日期:2003-02-10 00:00:00
abstract::Thyroid hormones, which play an important role in the development and regeneration of the nervous system, require the presence of specific nuclear T3 receptors (NT3R). In this study we provide evidence that NT3R expression by Schwann cells was up-regulated in response to a loss of axonal contact in vitro and in vivo. ...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199311180-00010
更新日期:1993-11-18 00:00:00
abstract::Postulating that central IL-1 is involved in the pathogenesis of cancer anorexia we measured IL-1 alpha in cerebrospinal fluid (CSF) from anorectic tumor bearing (TB) rats and non-tumor bearing controls, and correlated their CSF IL-1 alpha with food intake and tumor weight. Food intake in controls was significantly hi...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199503270-00011
更新日期:1995-03-27 00:00:00
abstract::The expression pattern of metabotropic glutamate receptor 1alpha (mGluR1alpha) was immunohistochemically investigated in substantia nigra dopaminergic neurons of the macaque monkey. In normal monkeys, mGluR1alpha immunoreactivity was weakly observed in the dorsal tier of the substantia nigra pars compacta (SNc-d) wher...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/01.wnr.0000074344.81633.e4
更新日期:2003-05-23 00:00:00
abstract::Bulbospinal C1 neurons are sympathoexcitatory and excited by substance P. However the substance P receptor (NK1 receptor), has been reported to be absent from the somata of C1 neurons. In this study, using double and triple labelling immunofluorescence and retrograde tracing, we provide evidence that the NK1 receptor ...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200112040-00012
更新日期:2001-12-04 00:00:00
abstract::Event-related potentials were recorded in healthy volunteers to test the accuracy of the human brain to extract, preattentively, auditory abstract rules. The abstract rule was determined by the frequency relationship between two pure tones forming a pair. The standard pairs had identical tone frequency, whereas the de...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200502280-00020
更新日期:2005-02-28 00:00:00
abstract::In situ hybridization and Northern blotting were used to study the expression of brain-derived neurotrophic factor (BDNF) mRNA in the rat brain following photochemical stroke. A focal thrombotic lesion of the sensorimotor cortex was produced by intravenously injecting the light-sensitive dye rose bengal and exposing t...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199206000-00004
更新日期:1992-06-01 00:00:00
abstract:OBJECTIVE:Cervical spondylotic myelopathy is regarded as a chronic, special incomplete spinal cord injury, so the sensory components transmitted to thalamus decreased after distal spinal cord injury, which lead the disturbance of thalamus-cortex circuits, which might explain the alterations of clinical function of cerv...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000001346
更新日期:2020-03-25 00:00:00