Abstract：
:Oropharyngeal candidiasis is a frequent opportunistic infection associated with immunocompromised hosts. Candida albicans is the principal species responsible for this infection. Production of interleukin-8 (IL-8), by oral epithelial cells can be expected to play a major role in the recruitment and activation of professional phagocytes at the infected site. The purpose of this study was to determine whether C. albicans triggers secretion of IL-8 by oral epithelial cells in vitro and investigate mechanisms of host cell-fungal interactions that trigger such responses. Oral epithelial cell lines (SCC4, SCC15, and OKF6/TERT-2) as well as primary gingival epithelial cells were used. Epithelial cells were cocultured with C. albicans, strains SC5314, ATCC28366 or ATCC32077, for 24-48 hr, and supernatants were analyzed for IL-8 content by ELISA. A germination-deficient mutant (efg1/efg1 cph1/cph1), otherwise isogenic to strain SC5314, was used to assess the requirement for germination in triggering IL-8 responses. In order to ascertain whether direct contact of yeast with host cells is required to trigger cytokine production, epithelial cells were separated from yeast using cell culture inserts. To test whether IL-8 secretion is dependent on IL-1alpha activity, epithelial cells were challenged with viable C. albicans in the presence or absence of neutralizing anti-IL-1alpha antibody or IL-1ra, and IL-8 secretion was measured in the supernatants. All cell lines and primary cultures responded to C. albicans with an increase in IL-8 secretion. IL-8 responses were contact-dependent, strain-specific, required yeast viability and germination into hyphae, and were in part autoregulated by IL-1alpha.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Dongari-Bagtzoglou A,Kashleva Hdoi
10.1016/s0882-4010(03)00004-4subject
Has Abstractpub_date
2003-04-01 00:00:00pages
169-77issue
4eissn
0882-4010issn
1096-1208pii
S0882401003000044journal_volume
34pub_type
杂志文章abstract：:Human leishmaniasis is a spectral disease that includes asymptomatic self-resolving infection, localized skin lesions, and progressive visceral leishmaniasis. With some overlap, visceral and cutaneous leishmaniasis are usually caused by different species of Leishmania. This review focuses on host responses to infectio...
journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
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abstract：:The genetic control of mouse susceptibility to Chlamydia psittaci was investigated after intravenous inoculation of 2 x 10(5) plaque-forming units (pfu). Splenic counts 6 days after inoculation gave the level of susceptibility. Results from inoculation of mice from H-2 congenic strains with three different genetic bac...
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journal_title：Microbial pathogenesis
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更新日期：2000-05-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2008.02.004
更新日期：2008-07-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104543
更新日期：2020-12-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(92)90034-l
更新日期：1992-07-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103625
更新日期：2019-10-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章,评审
doi：10.1016/j.micpath.2016.10.028
更新日期：2016-12-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2010.05.002
更新日期：2010-09-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章,评审
doi：10.1006/mpat.1994.1066
更新日期：1994-10-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.05.051
更新日期：2018-08-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2011.03.004
更新日期：2011-07-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2016.11.023
更新日期：2017-01-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104460
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2005.11.003
更新日期：2006-02-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(92)90049-t
更新日期：1992-04-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.08.035
更新日期：2017-10-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2010.06.006
更新日期：2010-12-01 00:00:00
abstract：:Uropathogenic Escherichia coli (UPEC) comprise a heterogeneous group of strains. In a previous epidemiological survey performed on 230 UPEC isolates, five virulence profiles were described, each one defined by the presence of some virulence determinants and by the absence of others. Phylogenetic groups and antibiotic ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2014.11.002
更新日期：2014-12-01 00:00:00
abstract：:Photobacterium damselae subsp. damselae has been known as an opportunistic pathogen in fish and mammals. Human infectious cases are often very serious and occasionally fatal. We previously reportedtwo fatal cases caused by this subspecies where the patients developed multiple organ failure within 20-36 h after the ons...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2008.04.008
更新日期：2008-08-01 00:00:00
abstract：:In recent year, propagation and resistance of pathogenic microorganisms (bacteria, fungi and virals) to common antimicrobial agents has led to serious health and food problems. Today, nanotechnology science and nanoparticles (NPs) have been identified as a new approach to deal with this problem because of their inhere...
journal_title：Microbial pathogenesis
pub_type： 杂志文章,评审
doi：10.1016/j.micpath.2018.08.008
更新日期：2018-10-01 00:00:00
abstract：OBJECTIVE:Prompt genotyping of Mycobacterium tuberculosis (M. tuberculosis) is crucial for improving molecular epidemiological investigation of tuberculosis (TB). METHODS:We performed a retrospective study to evaluate the use of 24 loci MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number of tandem-re...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2016.12.025
更新日期：2017-02-01 00:00:00
abstract：:A specific mistranslation mechanism for the replication of an infectious protein is described. The feedback mechanism requires the infectious agent to induce concerted frameshifts during the translation of a cellular gene. Each module of the tandem repeat region of the gene encoding the prion protein (PrP) associated ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章,评审
doi：10.1016/0882-4010(89)90098-3
更新日期：1989-04-01 00:00:00
