Mutation of purD and purF genes further attenuates Brucella abortus strain RB51.

Abstract:

:In the present study, transposon mutagenesis was used to further attenuate Brucella abortus RB51 vaccine strain. Two purD and purF mutants were constructed, characterized and evaluated for attenuation via intracellular survival in murine macrophage-like RAW264.7 and HeLa cells, and by clearance in BALB/c mice. The purD and purF mutants showed significantly decreased intracellular survival, and complementation of these mutants with intact copies of purD or purF genes of RB51 strain was able to restore these defects. In addition, the pur mutants presented significantly lowered persistence in mice. Immunization with purD and purF mutants protected mice against a challenge with the virulent B. abortus strain 544 at a level similar to that of the parent RB51. These data suggest that genes encoding the early stages of purine biosynthesis (purD and purF) are required for intracellular survival and virulence of B. abortus.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Truong QL,Cho Y,Barate AK,Kim S,Watarai M,Hahn TW

doi

10.1016/j.micpath.2014.12.003

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

1-7

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(14)00197-1

journal_volume

79

pub_type

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