当前位置： SCI文献检索 > MICROBIAL PATHOGENESIS期刊下所有文献 > Characterization of a DNA region containing 5'-(CAAT)(n)-3' DNA sequences involved in lipooligosaccharide biosynthesis in Haemophilus somnus.

Characterization of a DNA region containing 5'-(CAAT)(n)-3' DNA sequences involved in lipooligosaccharide biosynthesis in Haemophilus somnus.

Abstract：

:Repetitive tetranucleotide sequences of 5'-(CAAT)(n)-3' have been identified at the 5' end of an open reading frame (ORF) named lob1 from Haemophilus somnus strain 738. Based on sequence analysis, lob1 has 59% DNA homology to lex2B, which is involved in lipooligosaccharide (LOS) biosynthesis in H. influenzae. We now report that the number of 5'-CAAT-3' repeats in lob1 varied from 31-35, but that 94% of colonies contained 33 repeats of 5'-CAAT-3' downstream of two potential start codons, as determined by DNA sequence analysis of the 5'-CAAT-3' region from individual colonies. If transcription began with the start codon closest to the 5'-CAAT-3' repeats, a protein of 34.5 kDa would be encoded when 33 repeats were present. However, we could not establish a correlation between the number of 5'-CAAT-3' repeats in lob1 with a specific LOS electrophoretic profile or reactivity with two LOS monoclonal antibodies, indicating multiple genes control LOS phase variation in H. somnus. Complementation of strain 129Pt with lob1 containing 33 5 '-CAAT-3' repeats in shuttle vector pLS88 resulted in transformants 129Pt(pLSlob1-33A) and 129Pt(pLSlob1-33B), both of which demonstrated the same altered LOS electrophoretic profile. Unlike strain 129Pt, both transformants underwent limited LOS phase variation, which correlated with variation in the number of 5'-CAAT-3' repeats in pLSlob1-33. Nanoelectrospray-mass spectrometry of O-deacylated LOS indicated that transformant 129Pt(pLSlob1-33A) LOS was composed of a different distribution of glycoforms than LOS of the parent strain. The ratio of glucose to galactose changed from 1:2 in strain 129Pt LOS to 2:1 in transformant 129Pt(pLSlob1-33A) LOS, as determined by gas chromatography-mass spectrometry. Nuclear magnetic resonance spectroscopy confirmed and extended these observations. Transformant 129Pt(pLSlob1-33A) was constitutively more reactive in colony immunoblotting to polyclonal antiserum made to purified strain 738 LOS, and was more susceptible to complement-mediated killing in the presence of anti-738 LOS serum than parent strain 129Pt. Based on these results, Lob1 appears to be a phase variable galactosyl transferase involved in LOS biosynthesis in H. somnus.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

McQuiston JH,McQuiston JR,Cox AD,Wu Y,Boyle SM,Inzana TJ

doi

10.1006/mpat.1999.0351

subject

Has Abstract

pub_date

2000-05-01 00:00:00

pages

301-12

issue

5

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(99)90351-0

journal_volume

28

pub_type

杂志文章

相关文献

MICROBIAL PATHOGENESIS文献大全
  • Strategies and advancements in human microbiome description and the importance of culturomics.

    abstract：:The human microbiota gained a big interest among the scientific community with numerous studies being performed to better understand its role in health and diseases. Even with all the success achieved in studying the bacterial populations at the different body sites and its interaction among each other and with the ho...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104460

    authors： Bilen M

    更新日期：2020-12-01 00:00:00

  • The interaction of Escherichia coli with normal human serum: factors affecting the capacity of serum to mediate lipopolysaccharide release.

    abstract：:We previously demonstrated that incubation of E. coli in normal human serum (NHS) resulted in the release of a finite fraction (approximately 30%) of LPS from the bacterial outer membrane. In experiments reported here, we examined factors which may enhance or diminish the capacity of NHS to mediate this limited LPS re...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(88)90068-x

    authors： Tesh VL,Morrison DC

    更新日期：1988-03-01 00:00:00

  • Ginsenoside Rb 1: A novel therapeutic agent in Staphylococcusaureus-induced Acute Lung Injury with special reference to Oxidative stress and Apoptosis.

    abstract：:Acute lung injury (ALI) is considered as an uncontrolled inflammatory response that can leads to acute respiratory distress syndrome (ARDS), which limits the therapeutic strategies. Ginsenosides Rb1 (Rb1), an active ingredient obtained from Panax ginseng, possesses a broad range of pharmacological and medicinal proper...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104109

    authors： Shaukat A,Yang C,Yang Y,Guo YF,Jiang K,Guo S,Liu J,Zhang T,Zhao G,Ma X,Wu Z,Zhou Q,Akhtar M,Zahoor A,Umar T,Shaukat I,Hanif S,Rajput SA,Hassan M,Mehmood K,Hua Z,Xiaoyan W,Nannan Y,Deng G

    更新日期：2020-06-01 00:00:00

  • Quantitation of H. pylori cytotoxin mRNA by real-time RT-PCR shows a wide expression range that does not correlate with promoter sequences.

    abstract：:Up to 28-fold differences in vacA expression in Helicobacter pylori strains grown in vitro were demonstrated by real time quantitative RT-PCR. These large differences in expression were unrelated to putative -35 and -10 motifs or to other untranslated sequences upstream of the ATG start site. The lack of correlation b...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2004.06.003

    authors： Ayala G,Chihu L,Perales G,Fierros-Zárate G,Hansen LM,Solnick JV,Sánchez J

    更新日期：2004-09-01 00:00:00

  • Biofilm inhibitory effect of chlorhexidine conjugated gold nanoparticles against Klebsiella pneumoniae.

    abstract：:Klebsiella pneumoniae (K. pneumoniae) is one of the major pathogen associated with nosocomial infections, especially catheter associated urinary tract infections which involved biofilm formation. This study was designed to evaluate the antibiofilm efficacy of gold nanoparticle conjugated with chlorhexidine (Au-CHX) ag...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2016.06.016

    authors： Ahmed A,Khan AK,Anwar A,Ali SA,Shah MR

    更新日期：2016-09-01 00:00:00

  • Genetic analysis of the plasmid region controlling virulence in Yersinia enterocolitica 0:9 by Mini-Mu insertions and lac gene fusions.

    abstract：:Enteropathogenic strains of Yersinia enterocolitica harbor a virulence plasmid (pYVe plasmid) of 70 kilobases (kb) which specifies, at 37 degrees C, a calcium requirement for growth, autoagglutinability, resistance to the bactericidal activity of human serum, and the expression of outer membrane proteins (OMP). Some m...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(86)90067-7

    authors： Cornelis G,Sory MP,Laroche Y,Derclaye I

    更新日期：1986-08-01 00:00:00

  • Complete genome sequence of Bacillus licheniformis BL-010.

    abstract：:The biodegradation of Aflatoxin B1 (AFB1) is an industry of increasing importance. Bacillus licheniformis BL-010 was isolated from the aflatoxin contaminated corn feed storage, and was shown to degrade AFB1 efficiently. Here we present the complete genome sequence of BL-010, the genome comprises 4,287,714 bp in a circ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.03.037

    authors： He S,Feng K,Ding T,Huang K,Yan H,Liu X,Zhang Z

    更新日期：2018-05-01 00:00:00

  • Geniposide reduces Staphylococcus aureus internalization into bovine mammary epithelial cells by inhibiting NF-κB activation.

    abstract：:Staphylococcus aureus (S.aureus), a Gram-positive organism, is a frequent cause of subclinical mastitis. Geniposide, an iridoid glucoside isolated from the fruits of Gardenia jasminoides, has been reported to exhibit antibacterial and anti-inflammatory properties. The ability of S. aureus internalizing into bovine mam...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.10.002

    authors： Wei Z,Su K,Jiang P,Shi M,Wang J,Xie G

    更新日期：2018-12-01 00:00:00

  • Nanoparticles and their antimicrobial properties against pathogens including bacteria, fungi, parasites and viruses.

    abstract：:In recent year, propagation and resistance of pathogenic microorganisms (bacteria, fungi and virals) to common antimicrobial agents has led to serious health and food problems. Today, nanotechnology science and nanoparticles (NPs) have been identified as a new approach to deal with this problem because of their inhere...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,评审

    doi：10.1016/j.micpath.2018.08.008

    authors： Khezerlou A,Alizadeh-Sani M,Azizi-Lalabadi M,Ehsani A

    更新日期：2018-10-01 00:00:00

  • First whole genome sequencing of Russian isolate of Capnocytophaga canimorsus, opportunistic pathogen causing lethal sepsis.

    abstract：:Capnocytophaga canimorsus is a part of healthy oral flora of dogs and cats. However, when it is transmitted to human subjects via animal bites or scratches, it can cause severe complications like endocarditis or even lethal septic shock, especially in immunocompromised persons. In this study, we performed the first wh...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.10.020

    authors： Shelenkov AA,Karan LS,Mihaylova YV,Yanushevich YG,Shipulin GA,Shagin DA

    更新日期：2018-12-01 00:00:00

  • Preparation and evaluation of antibacterial potential of Pithecellobium dulce root extract against Gram positive and Gram negative bacteria.

    abstract：:In the present study hexane, benzene, ethyl acetate and ethanol extracts of Pithecellobium dulce root were prepared using soxhlet extractor. The extracts were evaluated for antibacterial activity against one Gram positive (Staphylococcus aureus) and three Gram negative (Acetobacter aceti, Acetobacter aceti, Klebsiella...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.01.013

    authors： Bhat MA,Malik RA,Prakash P,Lone AM

    更新日期：2018-03-01 00:00:00

  • Elevated neutrophil, macrophage and dendritic cell numbers characterize immune cell populations in mice chronically infected with Salmonella.

    abstract：:The present study characterizes immune cell populations in mice chronically infected with Salmonella. Mice were characterized as chronically infected based on persistently high titers of Salmonella-reactive immunoglobulins in the serum >6 months after a single oral dose of S. enterica serovar Typhimurium. These mice h...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2006.03.004

    authors： Johansson C,Ingman M,Jo Wick M

    更新日期：2006-08-01 00:00:00

  • Nucleotide sequence and expression of an extracellular hemolysin gene of Aeromonas hydrophila.

    abstract：:The extracellular hemolysin (AHH1) gene of Aeromonas hydrophila ATCC7966 was cloned into Charomid9-28 in Escherichia coli DH1, and its complete nucleotide sequence determined. Escherichia coli carrying this gene expressed an extracellular heat-labile hemolysin for rabbit red blood cells. The minimum size of the coding...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(91)90049-g

    authors： Hirono I,Aoki T

    更新日期：1991-09-01 00:00:00

  • Alternative method in Galleria mellonella larvae to study biofilm infection and treatment.

    abstract：:In vivo studies are crucial decision-maker step in order to translate in vitro data to an applied therapy. Considering this we describe a simple method that analyzes and quantifies biofilm formation inside the Galleria mellonella larvae. Toothbrush bristles were employed as an abiotic surface to mimic a medical device...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.103756

    authors： Campos-Silva R,Brust FR,Trentin DS,Macedo AJ

    更新日期：2019-12-01 00:00:00

  • Clinical relevance of Helicobacter pylori virulence factors in Iranian patients with gastrointestinal diseases.

    abstract：:Helicobacter pylori (H. pylori) usually colonizes the gastric mucosa of more than 50% of the human population, causing an infection that may appear in early childhood and can persist for life. H. pylori is suggested as the main cause of peptic ulcer and chronic gastritis. It is also associated with gastric cancer. Its...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,评审

    doi：10.1016/j.micpath.2016.09.016

    authors： Bagheri N,Azadegan-Dehkordi F,Rafieian-Kopaei M,Rahimian G,Asadi-Samani M,Shirzad H

    更新日期：2016-11-01 00:00:00

  • Characterization of classic and atypical serogroup O:11 Aeromonas: evidence that the surface array protein is not directly involved in mouse pathogenicity.

    abstract：:A number of different techniques were used to analyse classic and atypical serogroup O:11 Aeromonas isolates. Five of seven atypical O:11, S layer-negative strains lacking a homogeneous LPS side-chain pattern exhibited varying degrees of mouse pathogenecity. One virulent atypical strain (AH-77) synthesized a surface a...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(91)90067-k

    authors： Kokka RP,Vedros NA,Janda JM

    更新日期：1991-01-01 00:00:00

  • Macrophage--Mycobacterium tuberculosis interactions: role of complement receptor 3.

    abstract：:Tuberculosis is the leading infectious disease in the world. Mycobacterium tuberculosis, the causal agent of this disease, invades macrophages and can replicate inside them. Because invasion of macrophages is a critical step for establishing a mycobacterial infection, there is much interest in understanding the mechan...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,评审

    doi：10.1016/s0882-4010(03)00099-8

    authors： Velasco-Velázquez MA,Barrera D,González-Arenas A,Rosales C,Agramonte-Hevia J

    更新日期：2003-09-01 00:00:00

  • The correlation between intestinal dysbiosis and the development of ankylosing spondylitis.

    abstract：:The pathogenesis and development of ankylosing spondylitis (AS) is concealed and complicated. In recent years, alterations in gut microbiota of AS patients have been largely investigated, although the underlying mechanisms remain unclear. This article reviews the recent studies on changes of gut microbiota in AS patie...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,评审

    doi：10.1016/j.micpath.2019.04.038

    authors： Zhang L,Hu Y,Xu Y,Li P,Ma H,Li X,Li M

    更新日期：2019-07-01 00:00:00

  • The emergence of a novel recombinant porcine reproductive and respiratory syndrome virus with an amino acid insertion in GP5 protein.

    abstract：:As an economic devastating virus, porcine reproductive and respiratory syndrome virus (PRRSV) has spread globally, and seriously hinders the healthy development of the swine industry worldwide. In recent years, however, recombinant PRRSV strains are continuously emerging, resulting in the death of a large number of pi...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104573

    authors： Song Z,Zhang Q,Chen Y,Shen H,Yang G,Jiang P,Chen JL,Lin L

    更新日期：2020-12-01 00:00:00

  • Structure, function and regulation of the thermostable direct hemolysin (TDH) in pandemic Vibrio parahaemolyticus.

    abstract：:Vibrio parahaemolyticus is a leading cause of seafood-associated bacterial gastroenteritis. The pathogen produces the thermostable direct hemolysin (TDH), which is the sole cause of the Kanagawa phenomenon (KP), a special β-type haemolysis in the Wagatsuma agar. TDH also exerts several other biological activities, the...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,评审

    doi：10.1016/j.micpath.2018.07.021

    authors： Cai Q,Zhang Y

    更新日期：2018-10-01 00:00:00

  • Comparative genomic analyses of two novel qnrVC6 carrying multidrug-resistant Pseudomonas. spp strains.

    abstract：OBJECTIVES:This study focused on the comparative genomic analyses of two qnrVC6 carrying Pseudomonas spp. strains which might give us insights on the similarity and difference in the genomic contexts of qnrVC6 gene. METHODS:Comparative genomic analyses of the novel qnrVC6 carrying Pseudomonas spp. genomes with emphasi...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.07.026

    authors： Liu J,Yang L,Li L,Li B,Chen D,Xu Z

    更新日期：2018-10-01 00:00:00

  • The response regulator ArcA enhances biofilm formation in the vpsT manner under the anaerobic condition in Vibrio cholerae.

    abstract：:Vibrio cholerae, the agent of severe diarrheal disease cholera, is known to form biofilm to persist in the environmental and the host,s intestines. The bacteria execute a complex regulatory pathway producing virulence factors that allow colonization and cause disease in response to environmental signals in the intesti...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104197

    authors： Xi D,Yang S,Liu Q,Li Y,Li Y,Yan J,Wang X,Ning K,Cao B

    更新日期：2020-07-01 00:00:00

  • In vitro studies of epithelium-associated crystallization caused by uropathogens during urinary calculi development.

    abstract：:Infectious urinary stones account for about 10% of all urinary stones. In 50% of cases urolithiasis is a recurrent illness, which can lead to the loss of a kidney if not properly treated. One of the reasons for recurrence of the disease may be the ability of bacteria to invade urothelial cells, persist in the host cel...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2014.04.007

    authors： Torzewska A,Budzyńska A,Białczak-Kokot M,Różalski A

    更新日期：2014-06-01 00:00:00

  • Evidence for group A-related M protein genes in human but not animal-associated group G streptococcal pathogens.

    abstract：:Group G streptococci have on their surface antiphagocytic M protein-like antigens. To determine if these organisms have genes similar to the M protein genes of Streptococcus pyogenes (group A), DNA from independent group G isolates of human and animal origin were tested for homology to probes representing sequences en...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(87)90004-0

    authors： Simpson WJ,Robbins JC,Cleary PP

    更新日期：1987-11-01 00:00:00

  • Characterization of LIC11207, a novel leptospiral protein that is recognized by human convalescent sera and prevents apoptosis of polymorphonuclear leukocytes.

    abstract：:We report the study of a predicted outer-membrane leptospiral protein encoded by the gene lic11207. This protein is conserved in several pathogenic leptospiral strains but is absent in the saprophyte Leptospira biflexa. This putative outer-membrane protein has a domain of unknown function (DUF) 1565 found in several p...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2012.10.002

    authors： Pretre G,Lapponi MJ,Atzingen MV,Schattner M,Nascimento AL,Gómez RM

    更新日期：2013-03-01 00:00:00

  • myc-related proteins and DNA sequences in Trypanosoma brucei.

    abstract：:The cAMP content of Trypanosoma brucei increases in parallel with ascending mammalian parasitemia to very high levels just before differentiation of the long-slender to the short-stumpy bloodstream form. Because expression of myc oncogenes is required for vertebrate cells to interpret proliferation signals and decline...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(89)90110-1

    authors： Davis CE,Colmerauer ME,Kim CH,Matthews B,Guiney DG

    更新日期：1989-07-01 00:00:00

  • Phylogenetic analysis and molecular characteristics of 17 porcine reproductive and respiratory syndrome virus isolates in Southern China from 2010 to 2011.

    abstract：:We analyzed the complete genomic sequences of 17 porcine reproductive and respiratory syndrome virus (PRRSV) isolates from Southern China obtained between 2010 and 2011 and found that four of seven isolates from 2011 were closely related to the JXA1-R strain (vaccine virus of JXA1). This close relationship between fie...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2013.09.005

    authors： Zhang M,Xie J,Sun L,Cao Z,Gu H,Deng S,Chen Y,Cao Z,Tang F,Su S,Zhang G

    更新日期：2013-12-01 00:00:00

  • Identification and expression of nor efflux family genes in Staphylococcus epidermidis that act against gatifloxacin.

    abstract：:NorA, NorB, and NorC are efflux proteins in the Nor family that regulate the secretion of fluoroquinolones, and MgrA/NorR is a transcription factor of the Nor family. Overexpression of Nor family proteins provides fluoroquinolone resistance in Staphylococcus aureus. However, in coagulase-negative staphylococci (CNS), ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2012.03.001

    authors： Juárez-Verdayes MA,Parra-Ortega B,Hernández-Rodríguez C,Betanzos-Cabrera G,Rodríguez-Martínez S,Cancino-Diaz ME,Cancino-Diaz JC

    更新日期：2012-06-01 00:00:00

  • Preparation of porcine enterotoxigenic Escherichia coli (ETEC) ghosts and immunogenic analysis in a mouse model.

    abstract：:Enterotoxignenic Escherichia coli (ETEC)-associated colibacillosis causes high levels of morbidity and mortality in neonatal piglets. Vaccination is among effective strategy to fight against ETEC-related diseases. Bacterial ghosts (BGs) are empty bacterial envelopes, which substain subtle antigenic comformation in bac...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.11.015

    authors： Ran X,Chen X,Wang S,Chang C,Wen X,Zhai J,Ni H

    更新日期：2019-01-01 00:00:00

  • Identification of a maltose-inducible major outer membrane protein in Actinobacillus (Haemophilus) pleuropneumoniae.

    abstract：:The addition of maltose to the growth media of Actinobacillus pleuropneumonia (serotype 1) resulted in the induction of an outer membrane protein (OMP) with a molecular mass of 42 kDa. This protein had porin-like properties in that it was peptidoglycan-associated and was resistant to proteolysis by trypsin. A pleuropn...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(89)90084-3

    authors： Deneer HG,Potter AA

    更新日期：1989-06-01 00:00:00