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Quantitation of H. pylori cytotoxin mRNA by real-time RT-PCR shows a wide expression range that does not correlate with promoter sequences.

Abstract：

:Up to 28-fold differences in vacA expression in Helicobacter pylori strains grown in vitro were demonstrated by real time quantitative RT-PCR. These large differences in expression were unrelated to putative -35 and -10 motifs or to other untranslated sequences upstream of the ATG start site. The lack of correlation between promoter sequences and the vacA expression levels suggest the potential existence of a bacterial strain-specific factor, as earlier proposed by others on the basis of reporter gene fusions.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Ayala G,Chihu L,Perales G,Fierros-Zárate G,Hansen LM,Solnick JV,Sánchez J

doi

10.1016/j.micpath.2004.06.003

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

163-7

issue

3

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(04)00075-0

journal_volume

37

pub_type

杂志文章

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