Abstract:
:Phenotypic serum resistance of gonococci in urethral exudates is due to sialylation of lipopolysaccharide (LPS) by host cytidine 5'-monophospho-N-acetyl neuraminic acid (CMP-NANA). A surface structure was visible on gonococci [strain BS4 (agar)] that had been stained with ruthenium red after incubation with CMP-NANA. This structure was not visible after neuraminidase treatment, which released sialic acid but not LPS. The LPS profiles of strain BS4 (agar) had another in vivo-selected strain Gc40 (variant D1), were similar. A monoclonal antibody (mAb) which recognises epitope C on the LPS of 'capsulated' gonococci was shown by immunoblotting to react with several LPS components, including one of about 4.5 kDa which contains the probable site of sialylation by CMP-NANA. The reactions with the mAb were not affected by growing the strains with CMP-NANA nor by neuraminidase treatment of the sialylated LPS. The mAb also gold-labelled the surface of both strains before and after treatment with CMP-NANA. These data indicate that sialylation by CMP-NANA and staining with ruthenium red renders more visible the surface LPS which, sometimes in the past, has been seen as a 'capsule'.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Fox AJ,Curry A,Jones DM,Demarco de Hormaeche R,Parsons NJ,Cole JA,Smith Hdoi
10.1016/0882-4010(91)90050-ksubject
Has Abstractpub_date
1991-09-01 00:00:00pages
199-210issue
3eissn
0882-4010issn
1096-1208pii
0882-4010(91)90050-Kjournal_volume
11pub_type
杂志文章abstract::The genus Aeromonas comprises more than thirty Gram-negative bacterial species which mostly act as opportunistic microorganisms. These bacteria are distributed naturally in diverse aquatic ecosystems, where they are easily isolated from animals such as fish and crustaceans. A capacity for adaptation also makes Aeromon...
journal_title:Microbial pathogenesis
pub_type: 杂志文章,评审
doi:10.1016/j.micpath.2019.02.036
更新日期:2019-05-01 00:00:00
abstract::Vibrio cholerae, the agent of severe diarrheal disease cholera, is known to form biofilm to persist in the environmental and the host,s intestines. The bacteria execute a complex regulatory pathway producing virulence factors that allow colonization and cause disease in response to environmental signals in the intesti...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104197
更新日期:2020-07-01 00:00:00
abstract::Acanthamoeba are free-living, harmless organisms, however, given the opportunity and the appropriate conditions, they can cause painful, sight-threatening as well as fatal infections and, thus, are considered opportunistic pathogens. Acanthamoeba infections have become increasingly important in the past few years due ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章,评审
doi:10.1016/s0882-4010(03)00061-5
更新日期:2003-06-01 00:00:00
abstract::Janthinobacterium sp. B9-8, isolated from low temperature-sewage in Xinjiang, China, is capable of producing violacein, a promising antibiotic. Here we report the genome sequence of B9-8, which consist of 4,726,850 bp with a G + C content of 48.72%. The violacein biosynthesis gene cluster vioABCDE was identified and a...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.01.003
更新日期:2019-03-01 00:00:00
abstract::Our previous studies have shown that the adhesive ability of Enterococcus faecalis is dependent on the strain and is further modified by growth in serum. The data reported here demonstrate that E. faecalis adherence is mediated by carbohydrate residues present on the bacterial cell surface. Some of these (D-mannose an...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/0882-4010(91)90036-a
更新日期:1991-12-01 00:00:00
abstract::A virus inhibitor found in gastric secretions and in extracts from gastrointestinal tissues is described. The inhibitor shares a number of characteristics with the recently described contact-blocking virus inhibitor (CVI), which is produced by unstimulated cells in culture, and occurs naturally in some body fluids. Th...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/0882-4010(86)90048-3
更新日期:1986-06-01 00:00:00
abstract::Essential oils (EOs) obtained from aromatic plants are rich in natural components with interesting antimicrobial effects. The aim of this study was to evaluate the chemical composition of EOs extracted from Origanum majorana (OM-EO), Mentha suaveolens (MS-EO), Rosmarinus officinalis (RO-EO), Salvia officinalis (SO-EO)...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104510
更新日期:2020-12-01 00:00:00
abstract::Recent studies based on sequence divergence in the ospC gene have identified limited subpopulations of B. burgdorferi associated with invasive human disease. Spirochetes with certain OspC types never cause human disease, while some others cause local infection at the primary skin site but do not hematogenously dissemi...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/s0882-4010(03)00152-9
更新日期:2003-12-01 00:00:00
abstract::The Sm-like protein Hfq has been implicated in the regulation of sigmaS-dependent and sigmaS-independent genes in E. coli and in the regulation of virulence factors in both, Yersinia enterocolitica and Brucella abortus. Here, we have studied the effect of Hfq on virulence and stress response of Pseudomonas aeruginosa ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/s0882-4010(03)00149-9
更新日期:2003-11-01 00:00:00
abstract::Rhoptry proteins (ROPs) play a significant role in various stages of Toxoplasma gondii (T. gondii) life cycle, being critical for both invasion and intracellular survival. ROP38 is a key manipulator of host gene expression and has a function in tachyzoite to bradyzoite conversion. In this study, we've employed various...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104488
更新日期:2020-12-01 00:00:00
abstract::Biosynthesis of nanoparticles by fermented plants using microbes is an eco-friendly and cost-effective process. In this study, we used the fungus Aspergillus orayzae for the fermentation process. The aqueous extract of fermented Lupin (AEFL) possesses the ability to reduce selenium ion in the presence of gamma rays ev...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.06.013
更新日期:2018-09-01 00:00:00
abstract::Good vaccines should confer protection against specific pathogens in experimental and field conditions. However, some commercial vaccines are not capable to confer protection to animals, being inefficient in a bovine vaccine program. In this sense, the aim of this study was to evaluate the antibody levels involved in ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2017.10.042
更新日期:2018-01-01 00:00:00
abstract::The initiation of many infectious diseases involves specific adhesion of bacteria to host tissue proteins and carbohydrates. Staphylococcus aureus is known to bind specifically to several proteins in the extracellular matrix (ECM). We report the quantification of the collagen and fibronectin adhesin densities on the s...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.2000.0399
更新日期:2000-12-01 00:00:00
abstract::Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are two major omega-3 polyunsaturated fatty acids (n-3 PUFAs) with antimicrobial properties. In this study, we evaluated the potential antibacterial and antibiofilm activities of DHA and EPA against two periodontal pathogens, Porphyromonas gingivalis (P. gingi...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2016.08.025
更新日期:2016-10-01 00:00:00
abstract::A diarrheal isolate SSU of Aeromonas hydrophila produces a cytotoxic enterotoxin (Act) with cytotoxic, enterotoxic, and hemolytic activities. Our laboratory has characterized from the above Aeromonas strain, in addition to Act, the type 3- and T6-secretion systems and their effectors, as well as the genes shown to mod...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2008.08.007
更新日期:2008-11-01 00:00:00
abstract:BACKGROUND:Obesity is associated with alteration of the gut microbiota. In order to clarify the effect of Lactobacillus-containing probiotics (LCP) on weight we performed a meta-analysis of clinical studies and experimental models. We intended to assess effects by Lactobacillus species. METHODS:A broad search with no ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章,meta分析
doi:10.1016/j.micpath.2012.05.007
更新日期:2012-08-01 00:00:00
abstract::Bacillus anthracis secretes three major components, which assemble into two bipartite toxins: lethal toxin (LT), composed of lethal factor (LF) and protective antigen (PA) and edema toxin (ET), composed of edema factor (EF) and PA. EF is a potent calmodulin-dependent adenylate cyclase, which is internalized into the t...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2011.10.002
更新日期:2012-01-01 00:00:00
abstract::E. coli infections in avian species have become an economic threat to the poultry industry worldwide. Several factors have been associated with the virulence of E. coli in avian hosts, but no specific virulence gene has been identified as being entirely responsible for the pathogenicity of avian pathogenic E. coli (AP...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2008.08.005
更新日期:2008-11-01 00:00:00
abstract::Mycobacterium tuberculosis (Mtb) has coevolved with a human host to evade and exploit the immune system in multiple ways. Mtb is an enormously successful human pathogen that can remain undetected in hosts for decades without causing clinical disease. While tuberculosis (TB) represents a perfect prototype of host-patho...
journal_title:Microbial pathogenesis
pub_type: 杂志文章,评审
doi:10.1016/j.micpath.2019.03.003
更新日期:2019-05-01 00:00:00
abstract::Principle component analysis (PCA) was employed to examine the effect of the exerted treatments on the beef shelf life as well as discovering the correlations between the studied responses. Considering the variability of the dimensions of the responses, correlation coefficients were applied to form the matrix and extr...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2017.02.013
更新日期:2017-04-01 00:00:00
abstract::As an economic devastating virus, porcine reproductive and respiratory syndrome virus (PRRSV) has spread globally, and seriously hinders the healthy development of the swine industry worldwide. In recent years, however, recombinant PRRSV strains are continuously emerging, resulting in the death of a large number of pi...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104573
更新日期:2020-12-01 00:00:00
abstract::T lymphocyte-mediated immunity is important for resistance to Francisella tularensis. To characterize the specificity of this immunity, we used membrane proteins and two lipopolysaccharide (LPS) preparations. Both membrane proteins were heat-modifiable, as indicated by their migration in sodium dodecyl sulphate polyac...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/0882-4010(89)90021-1
更新日期:1989-12-01 00:00:00
abstract::Coccidiosis, caused by various species of genus Eimeria, is a major parasitic disease in chicken. The increasing resistance of these parasites to currently used anticoccidial drugs has stimulated the search for new methods of control. As part of this effort, a study was designed to see the anticoccidial effect of diff...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2017.01.053
更新日期:2017-03-01 00:00:00
abstract::The virulence of Staphylococcus aureus opsonized with an antiserum raised against a recombinant fibronectin-binding protein (FnBP) was compared with homologous, non-opsonized bacteria (treated with pre-immune serum) in a mouse mastitis model. Virulence was evaluated comparing the number of bacteria removed from the in...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1995.0044
更新日期:1995-07-01 00:00:00
abstract::A collection of Haemophilus ducreyi isolates were screened for the ability to bind to fibrinogen, fibronectin, collagen, gelatin and laminin by a particle agglutination test using latex beads coated with the individual proteins. Thirteen of 21 isolates reacted with all five extracellular matrix proteins. Binding of or...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/0882-4010(92)90034-l
更新日期:1992-07-01 00:00:00
abstract::Streptokinase (SK), a heterogeneous plasminogen (Pg) activator protein secreted by groups A, C and G streptococci (GAS/GCS/GGS) is a virulence factor composed of three structural domains; SKα/SKβ/SKγ. Phylogenetic analysis of the major variable region of SKβ (sk-V1; nucleotides 448-791; 343bp) which classifies the SK ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.103862
更新日期:2020-02-01 00:00:00
abstract::The rare minnow, Gobiocypris rarus, is small experimental fish proven to be sensitive to Grass Carp Reovirus (GCRV) infection. In present study we established a new cell (GrE) from eggs of G. rarus. GrE cells grew well at 28 °C in M199 medium containing 10% fetal bovine serum, and has been subcultured for over 70 pass...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.103715
更新日期:2019-11-01 00:00:00
abstract::Genital serovariants of Chlamydia trachomatis establish infection by attachment, entry and multiplication within human endometrial epithelial cells. In previous studies, a chlamydial recombinant Escherichia coli was identified which exhibited a specific adherent phenotype to endometrial epithelial cells closely resemb...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/s0882-4010(95)90281-3
更新日期:1995-10-01 00:00:00
abstract::We have recently shown that a mouse lung infection model resulting in acute pneumonia could be used for evaluating the protective immunity induced by mucosal vaccines against Vibrio cholerae. In order to gain insight and better understanding of the pathogenicity of V. cholerae infection, we identified and compared pro...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2015.07.002
更新日期:2015-09-01 00:00:00
abstract::This study was designed to screen for SCCmec types and to characterize the attachment site (attB) and universal insertion site (orfX) of SCCmec in a collection of 27 isolates (n = 11) methicillin resistant S. aureus and (n = 16) methicillin susceptible S. aureus isolates in Malaysia. Screening of SCCmec types and char...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.07.033
更新日期:2018-10-01 00:00:00