Abstract：
:The virulence of Staphylococcus aureus opsonized with an antiserum raised against a recombinant fibronectin-binding protein (FnBP) was compared with homologous, non-opsonized bacteria (treated with pre-immune serum) in a mouse mastitis model. Virulence was evaluated comparing the number of bacteria removed from the infected mammary glands and according to the type of lesions produced. The average number of bacteria recovered from the mammary glands inoculated with S. aureus opsonized with FnBP-antiserum was significantly lower (up to 10(7) cfu/ml) than the average number of bacteria recovered after inoculation with non-opsonized bacteria (up to 10(10) cfu/ml). Gross examination of infected mammary glands showed that 65% of glands infected with opsonized bacteria developed low grade/or had no pathological changes, and 35% developed severe mastitis whereas, 75% of glands inoculated with non-opsonized bacteria developed severe mastitis and 25% low grade mastitis or had no pathological changes. According to the histopathological examination eight out of 10 glands inoculated with opsonized bacteria produced disseminated focal necrosis or had no pathological changes and two glands produced non reactive necrotic lesions. In contrast, only three out of 10 glands inoculated with non-opsonized homologous bacteria developed disseminated focal necrosis and had no pathological changes while seven glands developed total necrosis.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Mamo W,Jonsson P,Müller HPdoi
10.1006/mpat.1995.0044subject
Has Abstractpub_date
1995-07-01 00:00:00pages
49-55issue
1eissn
0882-4010issn
1096-1208pii
S0882-4010(85)70044-7journal_volume
19pub_type
杂志文章abstract：:In vivo studies are crucial decision-maker step in order to translate in vitro data to an applied therapy. Considering this we describe a simple method that analyzes and quantifies biofilm formation inside the Galleria mellonella larvae. Toothbrush bristles were employed as an abiotic surface to mimic a medical device...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103756
更新日期：2019-12-01 00:00:00
abstract：:Janthinobacterium sp. B9-8, isolated from low temperature-sewage in Xinjiang, China, is capable of producing violacein, a promising antibiotic. Here we report the genome sequence of B9-8, which consist of 4,726,850 bp with a G + C content of 48.72%. The violacein biosynthesis gene cluster vioABCDE was identified and a...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.01.003
更新日期：2019-03-01 00:00:00
abstract：:CpG DNA is a potent activator of the innate immune system. Here the protective effects of CpG DNA are assessed against the facultative intracellular pathogen Francisella tularensis. Dosing of mice with CpG DNA provided protection against disease caused by F. tularensis subsp. holarctica live vaccine strain (LVS) but d...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2013.04.013
更新日期：2013-10-01 00:00:00
abstract：:This work illustrates a new role for the membranotropic peptide gH625 and its derivative gH625-GCGKKK in impairing formation of polymicrobial biofilms. Mixed biofilms composed of Candida and bacterial species cause frequently infections and failure of medical silicone devices and also show a major drug resistance than...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.09.027
更新日期：2018-12-01 00:00:00
abstract：:Yersina enterocolitica-like species have not been extensively studied regarding its pathogenic potential. This work aimed to assess the pathogenic potential of some Y. enterocolitica-like strains by evaluating the presence of virulence-related genes by PCR and their ability to adhere to and invade Caco-2 and HEp-2 cel...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.01.008
更新日期：2017-03-01 00:00:00
abstract：:Pyelonephritis induces an inflammatory process in the renal parenchyma, which may occur as a result of excessive reactive nitrogen intermediates (RNI) and reactive oxygen species (ROS) and/or impaired antioxidant capacity. In the present investigation, contribution of free radicals to the development of acute pyelonep...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2008.08.003
更新日期：2008-11-01 00:00:00
abstract：:Toxoplasma gondii is one of the most successful intracellular protozoan parasites in the world, which can infect most warm-blooded animals including foxes in the world and cause toxoplasmosis. This is the first meta-analysis to assess the overall prevalence and potential risk factors of T. gondii among foxes in the wo...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104699
更新日期：2020-12-28 00:00:00
abstract：:Quorum sensing (QS), a cell-to-cell communication mechanism present in many bacterial species plays a key role in regulating the virulence factor and biofilm formation in many pathogens, which causes severe public health risk. Therefore, interfering with QS mechanism offers an attractive strategy to combat bacterial i...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.01.015
更新日期：2017-03-01 00:00:00
abstract：:The objective of this paper was to evaluate NTPDase and 5'-nucleotidase activities in platelets of bovine with and without spleen and infected by Anaplasma marginale. Our results demonstrate that infection along with splenectomy is able of inducing a profile of cellular protection, which showed an increase in the degr...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2016.02.019
更新日期：2016-06-01 00:00:00
abstract：:Staphylococcus aureus is a bacterial pathogen causing approximately 80% of all cases of human osteomyelitis. This bacterium can adhere to and become internalized by osteoblasts and previous studies indicate that osteoblasts are active in the internalization process. In the current study, we examined the roles of micro...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1999.0272
更新日期：1999-06-01 00:00:00
abstract：:Mycobacterium tuberculosis (MTB) the causative organism of tuberculosis can remain dormant as a non-culturable organism, reactivate and cause disease in man and animals. There is a need for proof of viability of such organisms in order to understand the process of reactivation. PCR for bacterial DNA cannot distinguish...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.2000.0353
更新日期：2000-06-01 00:00:00
abstract：:Brucellosis, a disease caused by the gram-negative bacterium Brucella spp., is a widespread zoonosis that inflicts important animal and human health problems, especially in developing countries. One of the hallmarks of Brucella infection is its capacity to establish a chronic infection, characteristic that depends on ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2014.01.003
更新日期：2014-02-01 00:00:00
abstract：BACKGROUND:Coxiella burnetii and non-C. burnetii bacteria or endosymbiotic Coxiella-like were reported in various tick species. We aimed to detect C. burnetii within soft tick species, Argas persicus and Alveonasus canestrinii. METHODS:Argasid ticks were collected from different counties of Lorestan province, west of ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103902
更新日期：2020-02-01 00:00:00
abstract：:Escherichia coli O157:H7 is a food-borne pathogen that can cause hemorrhagic colitis and, occasionally, hemolytic uremic syndrome, a sequela of infection that can result in renal failure and death. Here we sought to model the pathogenesis of orally-administered E. coli O157:H7 in BALB/c mice with an intact intestinal ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2010.01.003
更新日期：2010-03-01 00:00:00
abstract：:The addition of maltose to the growth media of Actinobacillus pleuropneumonia (serotype 1) resulted in the induction of an outer membrane protein (OMP) with a molecular mass of 42 kDa. This protein had porin-like properties in that it was peptidoglycan-associated and was resistant to proteolysis by trypsin. A pleuropn...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(89)90084-3
更新日期：1989-06-01 00:00:00
abstract：:Naturally derived compounds represent a potential source of pharmacologically active drugs able to contrast different diseases, including gastric cancer, a multifactorial disease, in which the important role played by H. pylori infection has been demonstrated. Carexanes, stilbene derivatives, isolated from plants of t...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.05.007
更新日期：2017-07-01 00:00:00
abstract：:Most strains of Salmonella enterica subspecies enterica serotype typhimurium (S. typhimurium) naturally harbour a virulence plasmid which carries the salmonella plasmid virulence (spv) genes. However, isolates belonging to certain phage types are generally found without the plasmid. We have utilized a self-transferabl...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2004.02.005
更新日期：2004-06-01 00:00:00
abstract：:A trans-acting, positive effector of invasion plasmid antigen (Ipa) synthesis has been identified and mapped on the pWR100 invasion plasmid of Shigella flexneri serotype 5 (strain M90T-W). Recombinant plasmids carrying this regulatory gene, designated ipaR, were found to restore full virulence to a non-invasive ipaR::...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(90)90047-t
更新日期：1990-03-01 00:00:00
abstract：:Our previous studies have shown that the adhesive ability of Enterococcus faecalis is dependent on the strain and is further modified by growth in serum. The data reported here demonstrate that E. faecalis adherence is mediated by carbohydrate residues present on the bacterial cell surface. Some of these (D-mannose an...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(91)90036-a
更新日期：1991-12-01 00:00:00
abstract：:Influence of both recombinant interferon gamma (r-IFN-gamma) and monoclonal anti-IFN-gamma on murine Salmonella typhimurium infection was studied in vivo. As a challenge we used either a virulent or an avirulent strain of S. typhimurium. The avirulent strain is unable to replicate in the mouse spleen. The effect of IF...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(90)90077-4
更新日期：1990-02-01 00:00:00
abstract：:Three coagulase-negative Staphylococcus species (CNS) (Staphylococcus epidermidis, Staphylococcus xylosus and Staphylococcus hyicus), from the milk of cows with mastitis, were used to evaluate adherence to and internalization by bovine mammary epithelial cells, and to investigate involvement of host cell signal transd...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.2001.0465
更新日期：2001-11-01 00:00:00
abstract：:We report the study of a predicted outer-membrane leptospiral protein encoded by the gene lic11207. This protein is conserved in several pathogenic leptospiral strains but is absent in the saprophyte Leptospira biflexa. This putative outer-membrane protein has a domain of unknown function (DUF) 1565 found in several p...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2012.10.002
更新日期：2013-03-01 00:00:00
abstract：:A phage lambda mediated transduction system was devised to facilitate molecular analysis of Vibrio cholerae. A lamB expression plasmid, pAMH62 was introduced into Vibrio cholerae by conjugation. The resulting V. cholerae derivatives harboring pAMH62 produced substantial amounts of the LamB protein. This protein was pr...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(86)90053-7
更新日期：1986-06-01 00:00:00
abstract：:Outer surface protein E (OspE) is a complement factor H-binding virulence factor of borrelial subspecies. It is usually absent from in vitro grown Borrelia garinii, although in vivo B. garinii causes neuroborreliosis (NB). We analyzed the presence and sequence spectrum of the ospE genes in vivo in Borrelia spirochetes...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2010.06.006
更新日期：2010-12-01 00:00:00
abstract：INTRODUCTION:Tuberculosis (TB) is considered as a serious complication for organ transplant recipients; therefore, the detection and appropriate treatment of latent infection are recommended for preventing active TB infections in the future. The objective of this review is to conduct a systematic review and meta-analys...
journal_title：Microbial pathogenesis
pub_type： 杂志文章,meta分析
doi：10.1016/j.micpath.2018.09.040
更新日期：2018-12-01 00:00:00
abstract：:The present study was aimed to assess the prevalence and efficiency of techniques for the diagnosis of bovine tuberculosis (bTB) including enzyme-linked immunosorbent assay (ELISA), Gamma interferon assay (IFN-γ) and polymerase chain reaction (PCR) in comparison to skin tuberculin test and culture technique. A total o...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103574
更新日期：2019-09-01 00:00:00
abstract：:Heat-stable enterotoxins (ST) activate guanylyl cyclase in T84 cells, rapidly and specifically. Activation is monitored by cGMP production and occurs at lower concentrations of ST than required for eliciting fluid accumulation in suckling mice. Atrial natriuretic factor (ANF) neither activates guanylyl cyclase nor mod...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(92)90055-s
更新日期：1992-03-01 00:00:00
abstract：:The hemA mutation reduces the virulence of Salmonella typhimurium for mice by at least 10(7)-fold, as measured by change in LD50. The hemA mutation does not appear to affect killing of salmonella in mice. The salmonella with the hemA mutation persist in the spleen and liver for 2 to 3 weeks following intravenous injec...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(91)90033-7
更新日期：1991-10-01 00:00:00
abstract：BACKGROUND:Since the high morbidity and mortality of candidemia among cancer patients, the epidemiology has been underlined. In recent years, Candida species genotyping has been established, which could provide detail characteristics of epidemiology and has been underscored for candidemia preventing strategies. METHOD...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2015.03.005
更新日期：2015-04-01 00:00:00
abstract：:Pathogenic yersiniae undergo an established low calcium response (LCR) at 37 degrees C in Ca2+-deficient media characterized by restricted growth with synthesis of Lcr plasmid-encoded virulence functions. The latter include outer membrane peptides (Yops) known to undergo Pst plasmid-mediated post-translational degrada...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(89)90070-3
更新日期：1989-03-01 00:00:00
