Anti-quorum sensing and antibiofilm activities of Blastobotrys parvus PPR3 against Pseudomonas aeruginosa PAO1.

Abstract:

:The bacterial cell communication also termed as Quorum sensing (QS) system was involved in the expression of several virulence traits during Pseudomonas infection. The attenuating of this bacterial cell communication system is an attractive approach for the management of bacterial infections without the complication of resistance development. In this respect, the marine environment has gained significant attention due to its biodiversity and as a source of novel bioactive compounds. The present study aimed to screening effective QS inhibitors from marine associated fungal species for QS inhibitors. Twelve morphologically distinct fungal isolates were isolated from the wood of Avicennia marina from marine ecosystem. The anti-QS potential of fungal crude extract from was investigated in biosensor strain and test bacterium, Chromobacterium violaceum and Pseudomonas aeruginosa PAO1, respectively. Promising anti-QS activity was observed in the crude extract of one of the fungal isolate and identified by molecular characterization using internal transcribed spacer (ITS) region as Blastobotrys parvus PPR3. The anti-virulence and antibiofilm effects of ethyl acetate fractions from PPR3 against P. aeruginosa PAO1 were evaluated. The fungal metabolites responsible for the anti-QS activity of fungal crude extract was identified using gas chromatography-mass spectrometry (GC-MS). Furthermore, molecular docking studies were performed to understand the interaction of bioactive compounds with as receptors of P. aeruginosa PAO1. The crude extract of PPR3 showed reduction in different virulence traits of P. aeruginosa PAO1 such as production of pyocyanin, elastase, protease, chitinase, swimming and swarming motility, biofilm formation, exopolysaccharide production and alginate production at different sub-MIC concentrations. Interaction of bioactive metabolites with LasR and RhlR receptors of P. aeruginosa PAO1 was reported. The findings of the present study suggested that metabolites of B. parvus PPR3 interfere with QS system of P. aeruginosa PAO1 and alters the production of virulence factors.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Parasuraman P,Devadatha B,Sarma VV,Ranganathan S,Ampasala DR,Siddhardha B

doi

10.1016/j.micpath.2019.103811

subject

Has Abstract

pub_date

2020-01-01 00:00:00

pages

103811

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(19)30631-X

journal_volume

138

pub_type

杂志文章
  • Chemical composition, antioxidant, anticholinesterase, antimicrobial and antibiofilm activities of essential oil and methanolic extract of Anthemis stiparum subsp. sabulicola (Pomel) Oberpr.

    abstract::Anthemis species are traditionally used to treat infectious and inflammatory processes, among others clinical disturbances. In the current study, the chemical composition, the total phenolic and flavonoid contents, the antioxidant, anticholinesterase, antimicrobial, and antibiofilm activities of Anthemis stiparum subs...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.04.033

    authors: Chemsa AE,Zellagui A,Öztürk M,Erol E,Ceylan O,Duru ME,Lahouel M

    更新日期:2018-06-01 00:00:00

  • Inhibition of quorum sensing-controlled biofilm formation in Pseudomonas aeruginosa by quorum-sensing inhibitors.

    abstract::Antimicrobial therapy against extensively drug-resistant (XDR) P. aeruginosa biofilms is less efficient compared to the treatment of equal bacterial counts of free-floating planktonic cells, which has become a serious threat in hospital environment. P. aeruginosa regulate their cooperative activities and physiological...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2017.08.017

    authors: Kalaiarasan E,Thirumalaswamy K,Harish BN,Gnanasambandam V,Sali VK,John J

    更新日期:2017-10-01 00:00:00

  • Presence of F-like OriT base-pair sequence on the virulence plasmids of Salmonella serovars Gallinarum, Enteritidis, and Typhimurium, but absent in those of Choleraesuis and Dublin.

    abstract::The 90 kb virulence plasmids of Salmonella biosers Gallinarum and Pullorum (expressed as serovar Gallinarum) are non-conjugative. They were, however, found to be readily mobilized by IncFI and IncFIV plasmids, but not by conjugative IncA, IncB = C, IncH, IncL and IncM R plasmids. No virulence plasmids of serovars Chol...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1994.1048

    authors: Ou JT,Lin MY,Chao HL

    更新日期:1994-07-01 00:00:00

  • Influence of experimental Anaplasma marginale infection and splenectomy on NTPDase and 5'nucleotidase activities in platelets of cattle.

    abstract::The objective of this paper was to evaluate NTPDase and 5'-nucleotidase activities in platelets of bovine with and without spleen and infected by Anaplasma marginale. Our results demonstrate that infection along with splenectomy is able of inducing a profile of cellular protection, which showed an increase in the degr...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2016.02.019

    authors: Doyle RL,Oliveira CB,França RT,Doleski PH,Souza VC,Leal DBR,Martins JR,Lopes STA,Machado G,Da Silva AS,Andrade CM

    更新日期:2016-06-01 00:00:00

  • Nucleotide sequence variations within the lipopolysaccharide biosynthesis gene gseA (Kdo transferase) among the Chlamydia trachomatis serovars.

    abstract::The gene gseA, involved in the expression of the genus-specific epitope of chlamydial lipopolysaccharide (LPS), was analyzed by temperature gradient gel electrophoresis (TGGE) to visualize nucleotide sequence variations among the 15 serovars of Chlamydia trachomatis. Sequence analysis showed that the TGGE melting-prof...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1994.1055

    authors: Mamat U,Löbau S,Persson K,Brade H

    更新日期:1994-08-01 00:00:00

  • Acinetobacter nosocomialis secretes outer membrane vesicles that induce epithelial cell death and host inflammatory responses.

    abstract::Acinetobacter nosocomialis is an important nosocomial pathogen that causes a variety of opportunistic infections; however, pathogenesis of this microorganism has not yet been characterized. The aim of this study was to investigate the secretion of outer membrane vesicles (OMVs) from A. nosocomialis and to determine th...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2015.03.012

    authors: Nho JS,Jun SH,Oh MH,Park TI,Choi CW,Kim SI,Choi CH,Lee JC

    更新日期:2015-04-01 00:00:00

  • Microbial balance in the intestinal microbiota and its association with diabetes, obesity and allergic disease.

    abstract::Recent studies have been considered to symbiotic interactions of the human gastrointestinal microbiota and human lifestyle-related disorders. The human gastrointestinal microbiota continuously stimulates the immune system against opportunistic and pathogen bacteria from infancy. Changes in gastrointestinal microbiota ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审

    doi:10.1016/j.micpath.2018.11.031

    authors: Gholizadeh P,Mahallei M,Pormohammad A,Varshochi M,Ganbarov K,Zeinalzadeh E,Yousefi B,Bastami M,Tanomand A,Mahmood SS,Yousefi M,Asgharzadeh M,Kafil HS

    更新日期:2019-02-01 00:00:00

  • Langerhans cells (CD1a and CD207), dermal dendrocytes (FXIIIa) and plasmacytoid dendritic cells (CD123) in skin lesions of leprosy patients.

    abstract::The clinical course of infection with Mycobacterium leprae varies widely and depends on the pattern of the host immune response. Dendritic cells play an important role in the activation of the innate and adaptive immune system and seem to be essential for the development of the disease. To analyze the presence of epid...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2015.11.013

    authors: Hirai KE,Aarão TL,Silva LM,de Sousa JR,de Souza J,Dias LB Jr,Carneiro FR,Fuzii HT,Quaresma JA

    更新日期:2016-02-01 00:00:00

  • Genetic diversity and virulence characteristics of Staphylococcus aureus isolates from cases of bovine mastitis.

    abstract::Staphylococcus aureus is one of the major bacterial mastitis pathogens with significant effects on animal and human health. Some studies showed that S. aureus strains that infect different host species are genetically distinct, although most strains can infect a wide range of host species. However, there are no clearl...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104171

    authors: Vaughn JM,Abdi RD,Gillespie BE,Kerro Dego O

    更新日期:2020-07-01 00:00:00

  • Local transient induction of inflammatory cytokines after intranasal administration of recombinant Bordetella pertussis.

    abstract::Inflammatory cytokines have been described to play a critical role in the orientation and amplification of the IgA immune response. In this study, we show that the intranasal administration of a Bordetella pertussis strain expressing the protective antigen glutathione-S-transferase of Schistosoma mansoni (Sm28GST) ind...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1996.0130

    authors: Remoué F,Poulain-Godefroy O,Mielcarek N,Pierce R,Capron A,Locht C,Riveau G

    更新日期:1997-05-01 00:00:00

  • The emergence of a novel recombinant porcine reproductive and respiratory syndrome virus with an amino acid insertion in GP5 protein.

    abstract::As an economic devastating virus, porcine reproductive and respiratory syndrome virus (PRRSV) has spread globally, and seriously hinders the healthy development of the swine industry worldwide. In recent years, however, recombinant PRRSV strains are continuously emerging, resulting in the death of a large number of pi...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104573

    authors: Song Z,Zhang Q,Chen Y,Shen H,Yang G,Jiang P,Chen JL,Lin L

    更新日期:2020-12-01 00:00:00

  • The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins.

    abstract::Opportunistic infections, due to disease-related immunosuppression, constitute the major cause of death in American visceral leishmaniasis (AVL). Sera from these patients (AVL sera) non-specifically inhibit the in vitro proliferative response of normal human lymphocytes to lectins or antigens. In the present work, the...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2006.02.005

    authors: Soares NM,Ferraz TP,Nascimento EG,Carvalho EM,Pontes-de-Carvalho L

    更新日期:2006-06-01 00:00:00

  • Brucella abortus siderophore 2,3-dihydroxybenzoic acid (DHBA) facilitates intracellular survival of the bacteria.

    abstract::Siderophores are low molecular weight molecules that allow bacteria to acquire iron from host cell proteins. 2,3-dihydroxybenzoic acid (DHBA) is the only known siderophore produced by the intracellular pathogen Brucella abortus. Here its role in virulence was assessed by evaluating the ability of a mutant with a disru...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.2002.0500

    authors: Parent MA,Bellaire BH,Murphy EA,Roop RM 2nd,Elzer PH,Baldwin CL

    更新日期:2002-05-01 00:00:00

  • Interleukin-10 polymorphisms and susceptibility to ARV associated hepatotoxicity.

    abstract::Interleukin-10 (IL-10) is an anti-inflammatory cytokine associated with the inhibition of HIV replication. IL-10 polymorphisms were found to be linked to drug-induced hepatotoxicity. Hence we examined the prevalence of IL-10 (-819C/T,-1082A/G) polymorphisms in a total of 165 HIV patients which included 34 patients wit...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2019.103544

    authors: Singh H,Samani D,Nain S,Dhole TN

    更新日期:2019-08-01 00:00:00

  • A new subtype of a natural viral inhibitor (CVI) that is stable in the gastrointestinal tract.

    abstract::A virus inhibitor found in gastric secretions and in extracts from gastrointestinal tissues is described. The inhibitor shares a number of characteristics with the recently described contact-blocking virus inhibitor (CVI), which is produced by unstimulated cells in culture, and occurs naturally in some body fluids. Th...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(86)90048-3

    authors: Baron JL,Li JL,McKerlie ML,Shabot JM,Coppenhaver DH

    更新日期:1986-06-01 00:00:00

  • Elucidation of genetic relatedness of Magnaporthe grisea, an incitent of pearl millet blast disease by molecular markers associated with virulence of host differential cultivars.

    abstract::In recent years, blast disease caused by Magnaporthe grisea, an ascomycete fungus is becoming a serious threat to pearl millet crop in India and worldwide. Due to the increase in virulent races of pathogen, blast disease management strategies seemed to be very limited. Hence, unraveling the occurrence of blast isolate...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104533

    authors: Adhikari S,Joshi SM,Athoni BK,Patil PV,Jogaiah S

    更新日期:2020-12-01 00:00:00

  • Expression of Vibrio cholerae zonula occludens toxin and analysis of its subcellular localization.

    abstract::Vibrio cholerae elaborates zonula occludens toxin (Zot), a protein that increases the permeability of small intestinal mucosa by opening intercellular tight junctions. The zot gene is located, together with the genes encoding CT and Ace enterotoxins, within the genome of V. cholerae filamentous phage CTXsmall ef, Cyri...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1999.0312

    authors: Uzzau S,Cappuccinelli P,Fasano A

    更新日期:1999-12-01 00:00:00

  • The Trichomonas vaginalis phenotypically varying P270 immunogen is highly conserved except for numbers of repeated elements.

    abstract::The prominent and phenotypically variable immunogenic protein of Trichomonas vaginalis, termed P270, is present in all isolates. Most, if not all, patients make antibody to the DREGRD epitope contained in the 333 bp tandemly repeating element (TRE). The complete sequence of p270 of a fresh clinical isolate was recentl...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1999.0281

    authors: Musatovova O,Alderete JF

    更新日期:1999-08-01 00:00:00

  • Evidence for group A-related M protein genes in human but not animal-associated group G streptococcal pathogens.

    abstract::Group G streptococci have on their surface antiphagocytic M protein-like antigens. To determine if these organisms have genes similar to the M protein genes of Streptococcus pyogenes (group A), DNA from independent group G isolates of human and animal origin were tested for homology to probes representing sequences en...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(87)90004-0

    authors: Simpson WJ,Robbins JC,Cleary PP

    更新日期:1987-11-01 00:00:00

  • Integrons in uropathogenic Escherichia coli and their relationship with phylogeny and virulence.

    abstract::Uropathogenic Escherichia coli (UPEC) comprise a heterogeneous group of strains. In a previous epidemiological survey performed on 230 UPEC isolates, five virulence profiles were described, each one defined by the presence of some virulence determinants and by the absence of others. Phylogenetic groups and antibiotic ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2014.11.002

    authors: Poey ME,Laviña M

    更新日期:2014-12-01 00:00:00

  • Reduced virulence of a hfq mutant of Pseudomonas aeruginosa O1.

    abstract::The Sm-like protein Hfq has been implicated in the regulation of sigmaS-dependent and sigmaS-independent genes in E. coli and in the regulation of virulence factors in both, Yersinia enterocolitica and Brucella abortus. Here, we have studied the effect of Hfq on virulence and stress response of Pseudomonas aeruginosa ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/s0882-4010(03)00149-9

    authors: Sonnleitner E,Hagens S,Rosenau F,Wilhelm S,Habel A,Jäger KE,Bläsi U

    更新日期:2003-11-01 00:00:00

  • Occurrence of virulence factors in Cronobacter sakazakii and Cronobacter malonaticus originated from clinical samples.

    abstract:BACKGROUND:Cronobacter spp. are Gram-negative, facultative-anaerobic, non-spore forming, enteric coliform bacteria, which belongs to the Enterobacteriaceae family. Cronobacter spp. are opportunistic pathogens that have brought rare but life-threatening infections such as meningitis, necrotizing enterocolitis and bloods...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.12.011

    authors: Holý O,Cruz-Córdova A,Xicohtencatl-Cortes J,Hochel I,Parra-Flores J,Petrželová J,Fačevicová K,Forsythe S,Alsonosi A

    更新日期:2019-02-01 00:00:00

  • Study of serum bactericidal and splenic activity of Total-OMP- CagA combination from Brucella abortus and Helicobacter pylori in BALB/c mouse model.

    abstract:BACKGROUND:Brucella is a Gram-negative and facultative intracellular organism that causes brucellosis, a common zoonotic disease. Over 500,000 people are annually affected by brucellosis. Brucella is highly infectious through inhalation route; for this reason it is used for biological warfare aims. This study aimed to ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.04.050

    authors: Abadi AH,Mahdavi M,Khaledi A,Esmaeili SA,Esmaeili D,Sahebkar A

    更新日期:2018-08-01 00:00:00

  • Gut microbiome-based medical methodologies for early-stage disease prevention.

    abstract::Recent advancements highlight the important role of gut microbiome in human health. However, a variety of endogenous and exogenous factors, such as genes, foods, drugs, environmental pollutions, oxidative stress, etc., may interfere with the gut microbiome in vivo and increase risks of digestive system diseases, cardi...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审

    doi:10.1016/j.micpath.2017.02.024

    authors: Wang JZ,Du WT,Xu YL,Cheng SZ,Liu ZJ

    更新日期:2017-04-01 00:00:00

  • Pesticide degrading natural multidrug resistance bacterial flora.

    abstract::Multidrug-resistant (MDR) bacteria are a growing threat to humans across the world. Antibiotic resistance is a global problem that has developed through continuous antibiotic use, combinatorial antibiotic use, pesticide-antibiotic cross-resistance, and horizontal gene transfer, as well as various other modes. Pesticid...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审

    doi:10.1016/j.micpath.2017.12.013

    authors: Rangasamy K,Athiappan M,Devarajan N,Samykannu G,Parray JA,Aruljothi KN,Shameem N,Alqarawi AA,Hashem A,Abd Allah EF

    更新日期:2018-01-01 00:00:00

  • Protein D expression promotes the adherence and internalization of non-typeable Haemophilus influenzae into human monocytic cells.

    abstract::Protein D, having a glycerol-3-phosphodiester phosphodiesterase activity, is found at the surface of all Haemophilus influenzae strains and is a possible virulence factor. In the present study, the involvement of protein D in the entry of NTHi into human monocytic cells is reported. Primary monocytes and the monocytic...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.2001.0456

    authors: Ahrén IL,Janson H,Forsgren A,Riesbeck K

    更新日期:2001-09-01 00:00:00

  • Role of the two-component regulatory system arlRS in ica operon and aap positive but non-biofilm-forming Staphylococcus epidermidis isolates from hospitalized patients.

    abstract::The ica operon and aap gene are important factors for Staphylococcus epidermidis biofilm formation. However, we found 15 out of 101 S. epidermidis strains isolated from patients had both the ica operon and the aap gene in the genome but could not form biofilms (ica(+)aap(+)/BF(-) isolates). Compared with standard stra...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2014.09.013

    authors: Wu Y,Liu J,Jiang J,Hu J,Xu T,Wang J,Qu D

    更新日期:2014-11-01 00:00:00

  • Differential pathway network analysis used to identify key pathways associated with pediatric pneumonia.

    abstract::We aimed to identify key pathways to further explore the molecular mechanism underlying pediatric pneumonia using differential pathway network which integrated protein-protein interactions (PPI) data and pathway information. PPI data and pathway information were obtained from STRING and Reactome database, respectively...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2016.10.023

    authors: Yang JB,Luo R,Yan Y,Chen Y

    更新日期:2016-12-01 00:00:00

  • Visfatin regulates the production of lipopolysaccharide-induced inflammatory cytokines through p38 signaling in murine macrophages.

    abstract::Visfatin plays an important role in regulation of inflammatory cytokines. However, the role of visfatin under bacterial stress condition is not fully explored yet. Therefore, the present study was conducted for the better understanding of the regulation mechanism of visfatin on the production of inflammatory cytokines...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.02.002

    authors: Wu XT,Yang Z,Ansari AR,Xiao K,Pang XX,Luo Y,Song H

    更新日期:2018-04-01 00:00:00

  • Discrimination between apo and iron-loaded forms of transferrin by transferrin binding protein B and its N-terminal subfragment.

    abstract::Many pathogens of the Pasteurellaceae and Neisseriaceae possess a surface receptor that binds transferrin (Tf) as an initial step in an iron acquisition process. This receptor is comprised of two proteins, transferrin binding protein A (TbpA) and transferrin binding protein B (TbpB). Since the ability to recognize the...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1998.0226

    authors: Retzer MD,Yu Rh,Zhang Y,Gonzalez GC,Schryvers AB

    更新日期:1998-10-01 00:00:00