Abstract：
:The ica operon and aap gene are important factors for Staphylococcus epidermidis biofilm formation. However, we found 15 out of 101 S. epidermidis strains isolated from patients had both the ica operon and the aap gene in the genome but could not form biofilms (ica(+)aap(+)/BF(-) isolates). Compared with standard strain RP62A, the 15 ica(+)aap(+)/BF(-) isolates had similar growth curves and initial attachment abilities, but had much lower apparent transcription levels of the icaA gene and significantly less production of polysaccharide intercellular adhesion (PIA). Furthermore, the expression of accumulation-associated protein in ica(+)aap(+)/BF(-) isolates was much weaker than in RP62A. The mRNA levels of icaADBC transcription-related regulatory genes, including icaR, sarA, rsbU, srrA, arlRS and luxS, were measured in the 15 ica(+)aap(+)/BF(-) clinical isolates. The mRNA levels of arlR and rsbU in all of the ica(+)aap(+)/BF(-) isolates were lower than in RP62A at 4 h. At 10 h, 14/15 of the isolates showed lower mRNA levels of arlR and rsbU than shown by RP62A. However, expression of sarA, luxS, srrA and icaR varied in different ica(+)aap(+)/BF(-) isolates. To further investigate the role of arlRS in biofilm formation, we analyzed icaA, sarA and rsbU transcription, PIA synthesis, Aap expression and biofilm formation in an arlRS deletion mutant of S. epidermidis strain 1457 and all were much less than in the wild type strain. This is consistent with the hypothesis that ArlRS may play an important role in regulating biofilm formation by the ica(+)aap(+)/BF(-)S. epidermidis clinical isolates and operate via both ica-dependent and Aap-dependent pathways.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Wu Y,Liu J,Jiang J,Hu J,Xu T,Wang J,Qu Ddoi
10.1016/j.micpath.2014.09.013subject
Has Abstractpub_date
2014-11-01 00:00:00pages
89-98eissn
0882-4010issn
1096-1208pii
S0882-4010(14)00145-4journal_volume
76pub_type
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journal_title：Microbial pathogenesis
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doi：10.1016/j.micpath.2007.08.001
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103811
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journal_title：Microbial pathogenesis
pub_type： 杂志文章,评审
doi：10.1016/j.micpath.2016.10.028
更新日期：2016-12-01 00:00:00
abstract：:The bacterial phylum Synergistetes consists of Gram-negative anaerobes. Oral Synergistetes are divided in two main clusters, namely A and B. Increasing evidence demonstrates their involvement in etiology of oral infections, including apical periodontitis. This condition causes bone loss around the apex of the tooth, s...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2014.05.001
更新日期：2014-08-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.01.017
更新日期：2018-03-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2003.10.004
更新日期：2004-03-01 00:00:00
abstract：BACKGROUND:Brucella is a Gram-negative and facultative intracellular organism that causes brucellosis, a common zoonotic disease. Over 500,000 people are annually affected by brucellosis. Brucella is highly infectious through inhalation route; for this reason it is used for biological warfare aims. This study aimed to ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.04.050
更新日期：2018-08-01 00:00:00
abstract：:In order to characterize the toxicity of the hemorrhagic toxin of Clostridium sporogenes isolated from the rabbit with antibiotic-associated hemorrhagic diarrhoea, we studied the toxin productivity of C. sporogenes cultured in various media and the toxic effect of the partially purified preparation of the culture supe...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1996.0088
更新日期：1997-01-01 00:00:00
abstract：:The prominent and phenotypically variable immunogenic protein of Trichomonas vaginalis, termed P270, is present in all isolates. Most, if not all, patients make antibody to the DREGRD epitope contained in the 333 bp tandemly repeating element (TRE). The complete sequence of p270 of a fresh clinical isolate was recentl...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1999.0281
更新日期：1999-08-01 00:00:00
abstract：:The Aeromonas genus has several virulence factors associated with the development of diseases in aquatic organisms, leading to losses in aquaculture. One of these factors is the flagella's formation which allows the biofilm's formation that provides the microorganisms a greater pathogenicity, greater protection to cer...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.11.044
更新日期：2019-02-01 00:00:00
abstract：:This work illustrates a new role for the membranotropic peptide gH625 and its derivative gH625-GCGKKK in impairing formation of polymicrobial biofilms. Mixed biofilms composed of Candida and bacterial species cause frequently infections and failure of medical silicone devices and also show a major drug resistance than...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
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journal_title：Microbial pathogenesis
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doi：10.1006/mpat.2000.0435
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2016.01.010
更新日期：2016-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.micpath.2020.103986
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.04.025
更新日期：2019-06-01 00:00:00
abstract：:Quorum sensing (QS) is cell-cell communication mechanism mediated by signaling molecules known as autoinducers (AIs) that lead to differential gene expression. Salmonella is unable to synthesize the AI-1 acyl homoserine lactone (AHL), but is able to recognize AHLs produced by other microorganisms through SdiA protein....
journal_title：Microbial pathogenesis
pub_type： 杂志文章
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更新日期：2017-01-01 00:00:00
abstract：:In this experimental study, the Allium essential oil (AHEO) was extracted through the hydrodistillation method. AHEO components were identified through gas chromatography/mass spectrometry (GC/MS), and its antioxidant properties and total phenolic content were examined through the methods of 2,2-diphenyl-1-picrylhydra...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.11.055
更新日期：2018-01-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(91)90045-c
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abstract：:Salmonella spp. have been investigated as live vaccine vectors because they are heat stable and can elicit humoral, cellular, and secretory immune responses. However, the expression of some foreign antigens is toxic to bacterial vectors. We therefore studied an approach for the controlled expression of antigen in Salm...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1993.1060
更新日期：1993-08-01 00:00:00
abstract：:Edwardsiella tarda, an important fish pathogenic bacterium, could utilize type III secretion system (T3SS) to transfer multiple effector proteins into host cells during infection. EseG was identified to be an E. tarda T3SS effector, which could be injected by T3SS into non-phagocytic cells. Since E. tarda is a faculta...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2016.05.008
更新日期：2016-08-01 00:00:00
