Abstract:
:In this experimental study, the Allium essential oil (AHEO) was extracted through the hydrodistillation method. AHEO components were identified through gas chromatography/mass spectrometry (GC/MS), and its antioxidant properties and total phenolic content were examined through the methods of 2,2-diphenyl-1-picrylhydrazyl (DPPH), ß-carotene/linoleic acid inhibition and Folin-Ciocalteu, respectively. The antimicrobial effect of AHEO was evaluated on Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Streptococcus pyogenes, and Candida albicans through the methods of well diffusion, disk diffusion, minimum inhibitory concentration, and minimum bactericidal/fungicidal concentration. Phytochemical constituents (alkaloids, tannins, saponins, flavone and glycosides) were evaluated. 5-chloroorcylaldehyde with a percentage of 45.6% was the major compound of AHEO. Increasing concentration of AHEO had a significant effect (p ≤ 0.05) on inhibition zone diameter. The MICs of the AHEO varied from 0.25 mg/ml to 2 mg/ml. The MBCs/MFCs of the AHEO varied from 0.25 mg/ml to 4 mg/ml. The results of phytochemical screening of AHEO showed the existence of alkaloids, tannins, saponins, flavone and glycosides. There was also little difference between disk diffusion and well diffusion methods, and the data was well distributed throughout the X and Y components.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Alizadeh Behbahani B,Imani Fooladi AAdoi
10.1016/j.micpath.2017.11.055subject
Has Abstractpub_date
2018-01-01 00:00:00pages
299-303eissn
0882-4010issn
1096-1208pii
S0882-4010(17)31539-5journal_volume
114pub_type
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