Evaluation of phytochemical analysis and antimicrobial activities Allium essential oil against the growth of some microbial pathogens.

Abstract:

:In this experimental study, the Allium essential oil (AHEO) was extracted through the hydrodistillation method. AHEO components were identified through gas chromatography/mass spectrometry (GC/MS), and its antioxidant properties and total phenolic content were examined through the methods of 2,2-diphenyl-1-picrylhydrazyl (DPPH), ß-carotene/linoleic acid inhibition and Folin-Ciocalteu, respectively. The antimicrobial effect of AHEO was evaluated on Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Streptococcus pyogenes, and Candida albicans through the methods of well diffusion, disk diffusion, minimum inhibitory concentration, and minimum bactericidal/fungicidal concentration. Phytochemical constituents (alkaloids, tannins, saponins, flavone and glycosides) were evaluated. 5-chloroorcylaldehyde with a percentage of 45.6% was the major compound of AHEO. Increasing concentration of AHEO had a significant effect (p ≤ 0.05) on inhibition zone diameter. The MICs of the AHEO varied from 0.25 mg/ml to 2 mg/ml. The MBCs/MFCs of the AHEO varied from 0.25 mg/ml to 4 mg/ml. The results of phytochemical screening of AHEO showed the existence of alkaloids, tannins, saponins, flavone and glycosides. There was also little difference between disk diffusion and well diffusion methods, and the data was well distributed throughout the X and Y components.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Alizadeh Behbahani B,Imani Fooladi AA

doi

10.1016/j.micpath.2017.11.055

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

299-303

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(17)31539-5

journal_volume

114

pub_type

杂志文章
  • Impaired resistance to infection does not increase the virulence of Salmonella htrA live vaccines for mice.

    abstract::We have described a new class of live attenuated salmonella vaccines harbouring lesions in htrA, a stress protein gene previously. The virulence and invasiveness of Salmonella htrA mutants was investigated in three models of increased susceptibility to Salmonella infection. These included BALB/c mice, either given sub...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(92)90049-t

    authors: Strahan K,Chatfield SN,Tite J,Dougan G,Hormaeche CE

    更新日期:1992-04-01 00:00:00

  • Binding of Haemophilus ducreyi to extracellular matrix proteins.

    abstract::A collection of Haemophilus ducreyi isolates were screened for the ability to bind to fibrinogen, fibronectin, collagen, gelatin and laminin by a particle agglutination test using latex beads coated with the individual proteins. Thirteen of 21 isolates reacted with all five extracellular matrix proteins. Binding of or...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(92)90034-l

    authors: Abeck D,Johnson AP,Mensing H

    更新日期:1992-07-01 00:00:00

  • Effect of Clostridium perfringens type C on TLR4/MyD88/NF-κB signaling pathway in piglet small intestines.

    abstract::Clostridium perfringens (C. perfringens), a Gram-positive bacterium, is one of the main causing piglet diarrhea, which leads serious economic loss in the world swine industries. Generally, the innate immune response plays a critical role in host defense against pathogen invasion. TLR4, a member of the TLR (Toll-like r...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2019.103567

    authors: Shi H,Huang X,Yan Z,Yang Q,Wang P,Li S,Sun W,Gun S

    更新日期:2019-10-01 00:00:00

  • Regulation of NF-κB/MAPK signaling pathway attenuates the acute lung inflammation in Klebsiella pneumonia rats by mollugin treatment.

    abstract::Present study evaluates the protective effect of mollugin against Klebsiella pneumonia (KP) and also postulates the possible mechanism of its action. Klebsiella pneumoniae (2.4 × 108 CFU/ml) was used for the induction of KP. PMNs and WBC count was determined in the blood and bronchoalveolar lavage fluid (BALF) of Kleb...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2019.05.007

    authors: Zhang Y,Zhou S,Zhou J,Wang D,Zhou T

    更新日期:2019-07-01 00:00:00

  • Chlamydia pneumoniae facilitates monocyte adhesion to endothelial and smooth muscle cells.

    abstract::Chlamydia pneumoniae has been linked to atherosclerotic heart disease. However, there is a limited knowledge by which C. pneumoniae gain access to atheromatous lesions. The adhesion of C. pneumoniae -infected circulatory component(s) to endothelium and smooth muscle cells represents the first step in an inflammatory r...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.2000.0420

    authors: Kaul R,Wenman WM

    更新日期:2001-03-01 00:00:00

  • Identification of putative virulence-associated genes among Haemophilus parasuis strains and the virulence difference of different serovars.

    abstract::This study was aimed at determining virulence-associated genes among Haemophilus parasuis (H. parasuis) strains, and supplying for the Kielstein-Rapp-Gabrielson serotyping scheme. The subtractive fragments, obtained through suppression subtractive hybridization and reverse Southern blot hybridization, were found to en...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2014.10.001

    authors: Yu J,Wu J,Zhang Y,Du Y,Peng J,Chen L,Sun W,Cong X,Xu S,Shi J,Li J,Huang B,Zhu X,Wang J

    更新日期:2014-12-01 00:00:00

  • Visualizing fungal infections in living mice using bioluminescent pathogenic Candida albicans strains transformed with the firefly luciferase gene.

    abstract::Animal studies with Candida albicans have provided models for understanding fungal virulence and antifungal drug development. To non-invasively monitor long-term Candida murine infections, clinical isolates were stably transformed with a codon-optimized luciferase gene to constitutively express luciferase. Chronic sys...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2005.11.003

    authors: Doyle TC,Nawotka KA,Kawahara CB,Francis KP,Contag PR

    更新日期:2006-02-01 00:00:00

  • Pathophysiology of peste des petits ruminants in sheep (Dorper & Kajli) and goats (Boer & Beetal).

    abstract::Peste des petits ruminants (PPR), an economically important viral transboundary disease of small ruminants is not only prevalent in Pakistan but also in other countries where people rely on agriculture and animal products. The present study was aimed at describing the pathology and antigen localization in natural PPR ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.02.009

    authors: Khan A,Saleemi MK,Ali F,Abubakar M,Hussain R,Abbas RZ,Khan IA

    更新日期:2018-04-01 00:00:00

  • Green engineered biomolecule-capped silver and copper nanohybrids using Prosopis cineraria leaf extract: Enhanced antibacterial activity against microbial pathogens of public health relevance and cytotoxicity on human breast cancer cells (MCF-7).

    abstract::This research focused on green engineering and characterization of silver (PcAgNPs) and copper nanoparticles (PcCuNPs) using Prosopis cineraria (Pc) leaf extract prepared by using microwave irradiation. We studied their enhanced antimicrobial activity on human pathogens as well as cytotoxicity on breast cancer cells (...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2017.02.019

    authors: Jinu U,Gomathi M,Saiqa I,Geetha N,Benelli G,Venkatachalam P

    更新日期:2017-04-01 00:00:00

  • Characterization of a DNA region containing 5'-(CAAT)(n)-3' DNA sequences involved in lipooligosaccharide biosynthesis in Haemophilus somnus.

    abstract::Repetitive tetranucleotide sequences of 5'-(CAAT)(n)-3' have been identified at the 5' end of an open reading frame (ORF) named lob1 from Haemophilus somnus strain 738. Based on sequence analysis, lob1 has 59% DNA homology to lex2B, which is involved in lipooligosaccharide (LOS) biosynthesis in H. influenzae. We now r...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1999.0351

    authors: McQuiston JH,McQuiston JR,Cox AD,Wu Y,Boyle SM,Inzana TJ

    更新日期:2000-05-01 00:00:00

  • Deletions of chromosomal regions coding for fimbriae and hemolysins occur in vitro and in vivo in various extraintestinal Escherichia coli isolates.

    abstract::Fimbrial adhesins and hemolysins contribute to pathogenicity of extraintestinal Escherichia coli isolates causing urinary tract infections (UTI), sepsis and new born meningitis (NBM). Using gene cloning techniques and pulse field electrophoresis in combination with Southern hybridizations it was demonstrated that the ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(90)90048-u

    authors: Hacker J,Bender L,Ott M,Wingender J,Lund B,Marre R,Goebel W

    更新日期:1990-03-01 00:00:00

  • Apoptosis induced by bacterial pathogens.

    abstract::Programmed cell death is part of normal development and homeostasis. Apoptosis induced by bacteria appears to contribute to infectious diseases. Some bacteria produce toxins to kill host cells by the same pathway, apoptosis, through different mechanisms including pore formation, protein synthesis inhibition or adenyla...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审

    doi:10.1006/mpat.1994.1066

    authors: Chen Y,Zychlinsky A

    更新日期:1994-10-01 00:00:00

  • Opa (protein II) influences gonococcal organization in colonies, surface appearance, size and attachment to human fallopian tube tissues.

    abstract::Opa-expressing variants of Neisseria gonorrhoeae strain F62-SF and an Opa- variant, all non-piliated, were examined for differences in the interaction of the bacteria within colonies and in attachment to and damage of human fallopian tube mucosa. Expression of certain Opas was associated with the formation of transpar...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(90)90037-q

    authors: Dekker NP,Lammel CJ,Mandrell RE,Brooks GF

    更新日期:1990-07-01 00:00:00

  • Laying hens fed mycotoxin-contaminated feed produced by Fusarium fungi (T-2 toxin and fumonisin B1) and Saccharomyces cerevisiae lysate: Impacts on poultry health, productive efficiency, and egg quality.

    abstract::Mycotoxins represent substantial challenges to the farming industry. These include toxins produced by Fusarium fungi, particularly trichothecenes (toxin T-2) and fumonisin (FB1). In the present study, we determined the effects of addition on Saccharomyces cerevisiae lysate (SCL) added to feed contaminated with T-2 and...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104517

    authors: Dazuk V,Boiago MM,Rolim G,Paravisi A,Copetti PM,Bissacotti BF,Morsch VM,Vedovatto M,Gazoni FL,Matte F,Gloria EM,Da Silva AS

    更新日期:2020-12-01 00:00:00

  • Human endogenous retrovirus env genes: Potential blood biomarkers in lung cancer.

    abstract::Lung cancer, the leading cause of cancer mortality, needs urgent development of newly qualified diagnostic and therapeutic biomarkers. Recently, Human Endogenous Retroviruses (HERVs) have been introduced for cancer diagnosis. In this case-control study, we have collected blood samples from 60 lung cancer patients and ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2017.12.040

    authors: Zare M,Mostafaei S,Ahmadi A,Azimzadeh Jamalkandi S,Abedini A,Esfahani-Monfared Z,Dorostkar R,Saadati M

    更新日期:2018-02-01 00:00:00

  • Inhibitory effects of thymol on the cytotoxicity and inflammatory responses induced by Staphylococcus aureus extracellular vesicles in cultured keratinocytes.

    abstract::Staphylococcus aureus extracellular vesicles (EVs) deliver effector molecules to host cells and induce host cell pathology. This study investigated the disruption of S. aureus EVs by thymol along with its inhibitory effects on the cytotoxicity and inflammatory responses induced by EVs derived from two different S. aur...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2019.103603

    authors: Kwon HI,Jeong NH,Kim SY,Kim MH,Son JH,Jun SH,Kim S,Jeon H,Kang SC,Kim SH,Lee JC

    更新日期:2019-09-01 00:00:00

  • Need for TolC, an Escherichia coli outer membrane protein, in the secretion of heat-stable enterotoxin I across the outer membrane.

    abstract::Escherichia coli heat-stable enterotoxin Ip (STIp) is a typical extracellular toxin consisting of 18 amino acid residues synthesized as a precursor of pre (amino acid residues 1 to 19), pro (amino acid residues 20 to 54), and mature (amino acid residues 55 to 72) regions. STIp synthesized in the cytoplasm must cross t...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1998.0211

    authors: Yamanaka H,Nomura T,Fujii Y,Okamoto K

    更新日期:1998-09-01 00:00:00

  • Monoclonal antibodies recognizing the Enterococcus faecalis collagen-binding MSCRAMM Ace: conditional expression and binding analysis.

    abstract::Enterococci are opportunistic pathogens known to cause numerous clinical infections and complications in humans. Adhesin-mediated binding to extracellular matrix (ECM) proteins of the host is thought to be a crucial step in the pathogenesis of these bacterial infections. Adhesin of collagen from Enterococcus faecalis ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2007.03.003

    authors: Hall AE,Gorovits EL,Syribeys PJ,Domanski PJ,Ames BR,Chang CY,Vernachio JH,Patti JM,Hutchins JT

    更新日期:2007-08-01 00:00:00

  • Essential role of the glucosyltransferase activity in Clostridium difficile toxin-induced secretion of TNF-alpha by macrophages.

    abstract::Clostridium difficile causes serious and potentially fatal inflammatory diseases of the colon. Two large protein toxins, TcdA and TcdB, have been clearly implicated in pathogenesis. The goal of this study was to determine whether the glucosyltransferase activity of the toxins is critical for the induction of tumor nec...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2009.03.002

    authors: Sun X,He X,Tzipori S,Gerhard R,Feng H

    更新日期:2009-06-01 00:00:00

  • Identification of a Burkholderia mallei polysaccharide gene cluster by subtractive hybridization and demonstration that the encoded capsule is an essential virulence determinant.

    abstract::Little is known about the virulence factors of Burkholderia mallei, the etiologic agent of glanders. We employed subtractive hybridization to identify genetic determinants present in B. mallei but not in Burkholderia thailandensis, a non-pathogenic soil microbe. Three subtractive hybridization products were mapped to ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.2000.0430

    authors: DeShazer D,Waag DM,Fritz DL,Woods DE

    更新日期:2001-05-01 00:00:00

  • Indole enhances acid resistance in Escherichia coli.

    abstract::As a stationary-phase signal, indole is secreted in large quantities by Escherichia coli on enriched media and has been shown to control several genes; however, its impact on acid resistance remains to be studied in detail. Real-time quantitative reverse transcription-polymerase chain reaction analysis revealed that i...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2010.05.002

    authors: Hirakawa H,Hayashi-Nishino M,Yamaguchi A,Nishino K

    更新日期:2010-09-01 00:00:00

  • Influence of mycobacterial virulence and culture condition on gamma delta T cell activation.

    abstract::Activation of human gamma delta T cells by culture supernatants of virulent and avirulent mycobacteria was examined. The stimulatory potential of mycobacteria was influenced by the type of culture media and independent from their virulence. Activation of gamma delta T cells by phagocytes infected with viable virulent ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1997.0187

    authors: Schoel B,Kaufmann SH

    更新日期:1998-03-01 00:00:00

  • Mannosylated lipoarabinomannan balances apoptosis and inflammatory state in mycobacteria-infected and uninfected bystander macrophages.

    abstract::To assess the role of mannosylated lipoarabinomannan (ManLAM) in the inflammatory and apoptotic response of mycobacteria-infected and uninfected, bystander cells we applied a mouse macrophage model of infection with avirulent strains--Mycobacterium bovis BCG, Mycobacterium tuberculosis (MTB) H37Ra and compared with a ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2011.03.004

    authors: Wojtas B,Fijalkowska B,Wlodarczyk A,Schollenberger A,Niemialtowski M,Hamasur B,Pawlowski A,Krzyzowska M

    更新日期:2011-07-01 00:00:00

  • GroEL PCR- RFLP - An efficient tool to discriminate closely related pathogenic Vibrio species.

    abstract::Vibrio sp. are autochthonous to marine and estuarine waters. Several species of Vibrio are pathogens. It is of utmost importance to detect and discriminate the Vibrio sp. that are often involved in food and water borne infections. Since 16S rRNA based identification has limited utility in differentiating the closely r...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2017.02.029

    authors: Silvester R,Alexander D,Antony AC,Hatha M

    更新日期:2017-04-01 00:00:00

  • Rhodomyrtone decreases Staphylococcus aureus SigB activity during exponentially growing phase and inhibits haemolytic activity within membrane vesicles.

    abstract::Sigma factor B (SigB) controls the expression of Staphylococcus aureus genes including virulence factors and plays a role in the bacterial secretion system through membrane vesicle production. Inhibition of SigB could attenuate SigB dependent virulence and secretion system. The objective of this study was to determine...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.12.019

    authors: Mitsuwan W,Jiménez-Munguía I,Visutthi M,Sianglum W,REIPI\/GEIH Study Group.,Rodríguez-Ortega MJ,Voravuthikunchai SP

    更新日期:2019-03-01 00:00:00

  • Transcript analysis of nrrF, a Fur repressed sRNA of Neisseria gonorrhoeae.

    abstract::Like most microorganisms, Neisseria gonorrhoeae alters gene expression in response to iron availability. The ferric uptake regulator Fur has been shown to be involved in controlling this response, but the extent of this involvement remains unknown. It is known that in addition to working directly to repress gene expre...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2008.12.003

    authors: Ducey TF,Jackson L,Orvis J,Dyer DW

    更新日期:2009-03-01 00:00:00

  • Characterization of classic and atypical serogroup O:11 Aeromonas: evidence that the surface array protein is not directly involved in mouse pathogenicity.

    abstract::A number of different techniques were used to analyse classic and atypical serogroup O:11 Aeromonas isolates. Five of seven atypical O:11, S layer-negative strains lacking a homogeneous LPS side-chain pattern exhibited varying degrees of mouse pathogenecity. One virulent atypical strain (AH-77) synthesized a surface a...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(91)90067-k

    authors: Kokka RP,Vedros NA,Janda JM

    更新日期:1991-01-01 00:00:00

  • Brucella abortus siderophore 2,3-dihydroxybenzoic acid (DHBA) facilitates intracellular survival of the bacteria.

    abstract::Siderophores are low molecular weight molecules that allow bacteria to acquire iron from host cell proteins. 2,3-dihydroxybenzoic acid (DHBA) is the only known siderophore produced by the intracellular pathogen Brucella abortus. Here its role in virulence was assessed by evaluating the ability of a mutant with a disru...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.2002.0500

    authors: Parent MA,Bellaire BH,Murphy EA,Roop RM 2nd,Elzer PH,Baldwin CL

    更新日期:2002-05-01 00:00:00

  • Comparison of the pulmonary response against lethal and non-lethal intranasal challenges with two different pneumococcal strains.

    abstract::The differences between the immune response elicited during a self-limiting and a life-threatening lung infection with Streptococcus pneumoniae was analyzed in a mouse model of intranasal challenge using two different pneumococcal strains. M10, a serotype 11A strain, induced an early response within the first 12h afte...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2009.05.005

    authors: Ferreira DM,Moreno AT,Cianciarullo AM,Ho PL,Oliveira ML,Miyaji EN

    更新日期:2009-09-01 00:00:00

  • Occurrence of virulence factors in Cronobacter sakazakii and Cronobacter malonaticus originated from clinical samples.

    abstract:BACKGROUND:Cronobacter spp. are Gram-negative, facultative-anaerobic, non-spore forming, enteric coliform bacteria, which belongs to the Enterobacteriaceae family. Cronobacter spp. are opportunistic pathogens that have brought rare but life-threatening infections such as meningitis, necrotizing enterocolitis and bloods...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.12.011

    authors: Holý O,Cruz-Córdova A,Xicohtencatl-Cortes J,Hochel I,Parra-Flores J,Petrželová J,Fačevicová K,Forsythe S,Alsonosi A

    更新日期:2019-02-01 00:00:00