Abstract：
:Enterococci are opportunistic pathogens known to cause numerous clinical infections and complications in humans. Adhesin-mediated binding to extracellular matrix (ECM) proteins of the host is thought to be a crucial step in the pathogenesis of these bacterial infections. Adhesin of collagen from Enterococcus faecalis (Ace) is a cell-wall anchored protein of E. faecalis that has been shown to be important for bacterial binding to the ECM. In this report, we characterize the conditions for Ace expression and demonstrate Ace binding to mammalian epithelial and endothelial cells as well as to collagens found in the ECM. To further characterize Ace expression and function, we report the generation of a panel of monoclonal antibodies (mAbs) directed against this important E. faecalis virulence factor. Through the use of multiple in vitro assays, surface plasmon resonance and flow cytometry, we have characterized this panel of mAbs which may prove to be not only beneficial in studies that address the precise biological role of adhesion of E. faecalis, but may also serve as beneficial therapeutic agents against E. faecalis infections.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Hall AE,Gorovits EL,Syribeys PJ,Domanski PJ,Ames BR,Chang CY,Vernachio JH,Patti JM,Hutchins JTdoi
10.1016/j.micpath.2007.03.003subject
Has Abstractpub_date
2007-08-01 00:00:00pages
55-66issue
2-3eissn
0882-4010issn
1096-1208pii
S0882-4010(07)00034-4journal_volume
43pub_type
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journal_title：Microbial pathogenesis
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doi：10.1016/j.micpath.2017.11.023
更新日期：2017-12-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1994.1023
更新日期：1994-03-01 00:00:00
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doi：10.1006/mpat.1999.0272
更新日期：1999-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.micpath.2016.11.023
更新日期：2017-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.micpath.2016.12.025
更新日期：2017-02-01 00:00:00
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更新日期：2008-11-01 00:00:00
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
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更新日期：1998-10-01 00:00:00
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更新日期：2014-12-01 00:00:00
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更新日期：1989-06-01 00:00:00
