Abstract:
:A common feature of many chlamydial infections is that they are often asymptomatic and may persist for long periods of time if left untreated. In addition to the well recognized lytic stage of the chlamydial developmental cycle, evidence is now emerging to support a persistent phase in the cycle in which the reticulate bodies are morphologically abnormal, viable but non-infectious and presumably also have altered gene expression patterns. We used an RT-PCR approach to study the differential levels of gene transcription for 14 genes (16SrRNA, ompA, ompB, omcB, 76 kDa, gseA, pmp1, gltX, hsp60, yaeT, pyk, nlpD, Cpn0585, Cpn1046) between normal and IFN-gamma treated Chlamydia pneumoniae cell cultures. Even though the level of morphologically abnormal reticulate bodies in our IFN-gamma treated cultures was low (approximately 10% morphologically discernible, although presumably a larger percentage were in the persistent state but not yet morphologically altered) we identified five genes (ompA, ompB, pyk, nlpD, Cpn0585) that were clearly upregulated when compared to normal cultures. This gene transcript profile may be characteristic of a general stress state in Chlamydia, induced by IFN-gamma treatment in this case, but perhaps more widely induced in other in vitro and in vivo situations.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Mathews S,George C,Flegg C,Stenzel D,Timms Pdoi
10.1006/mpat.2000.0435subject
Has Abstractpub_date
2001-06-01 00:00:00pages
337-45issue
6eissn
0882-4010issn
1096-1208pii
S0882-4010(00)90435-2journal_volume
30pub_type
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2004.02.005
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2009.08.005
更新日期:2009-11-01 00:00:00
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doi:10.1016/j.micpath.2011.07.007
更新日期:2011-11-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
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journal_title:Microbial pathogenesis
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doi:10.1006/mpat.1999.0312
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pub_type: 杂志文章
doi:10.1016/j.micpath.2017.02.042
更新日期:2017-04-01 00:00:00
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doi:10.1016/j.micpath.2020.104517
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2017.09.047
更新日期:2017-11-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104558
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1994.1048
更新日期:1994-07-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章,评审
doi:10.1016/j.micpath.2020.104620
更新日期:2020-11-16 00:00:00
abstract::A novel N-acyl substituted indole-linked benzimidazoles and naphthoimidazoles were synthesized. Their chemical structures were confirmed using spectroscopic tools including 1H NMR, 13C NMR and CHN-elemental analyses. Anti inflammatory activity for all target compounds was evaluated in-vitro. The synthesized compounds ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2017.12.021
更新日期:2018-01-01 00:00:00