当前位置： SCI文献检索 > MICROBIAL PATHOGENESIS期刊下所有文献 > Structural basis for isoniazid resistance in KatG double mutants of Mycobacterium tuberculosis.

Structural basis for isoniazid resistance in KatG double mutants of Mycobacterium tuberculosis.

Abstract：

:The failure of drugs for effective treatment against infectious diseases can be attributed to resistant forms of causative agents. The evasive nature of Mycobacterium tuberculosis is partly associated to its physical features, such as having a thick cell wall and incorporation of beneficial mutations leading to drug resistance. The pro drug Isoniazid (INH) interacts with an enzyme catalase peroxidase to get converted into its active form and upon activation stops the cell wall synthesis thus killing the Mycobacterium. The most common mutation i.e. S315T leads to high degree of drug resistance by virtue of its position in the active site. Here, we have characterized the prominent attributes of two double mutant isolates S315 T/D194G and S315T/M624V which are multi drug resistant and extremely drug resistant, respectively. Protein models were generated using the crystal structure which were then subjected to energy minimization and long term molecular dynamics simulations. Further, computational analysis showed decreasing ability of INH binding to the mutants in order of: Native > S315T/D194G > S315T/M624V. Also, a trend was observed that as the docking score and binding area decreased, there was a significant increase in the distortion of the 3D geometry of the mutants as observed by PCA analysis.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Rabha A,Singh A,Grover S,Kumari A,Pandey B,Grover A

doi

10.1016/j.micpath.2019.02.003

subject

Has Abstract

pub_date

2019-04-01 00:00:00

pages

152-160

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(18)31495-5

journal_volume

129

pub_type

杂志文章

相关文献

MICROBIAL PATHOGENESIS文献大全
  • Prevalence of active tuberculosis infection in transplant recipients: A systematic review and meta-analysis.

    abstract：INTRODUCTION:Tuberculosis (TB) is considered as a serious complication of organ transplant; therefore, the detection and appropriate treatment of active TB infection is highly recommended for the reduction of mortality in the future. The aim of this review was to conduct a systematic review and meta-analysis assessing ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.micpath.2019.103894

    authors： Mamishi S,Pourakbari B,Moradzadeh M,van Leeuwen WB,Mahmoudi S

    更新日期：2020-02-01 00:00:00

  • Experimental infection of golden hamsters with Guama virus (Peribunyaviridae, Orthobunyavirus).

    abstract：:The Guama virus (GMAV) is a member of Peribunyaviridae family, Orthobunyavirus genus. Several strains of the virus were isolated in South and Central Americas from several hosts, such as humans, wild animals, including nonhuman primates, wild rodents and mosquitoes as well as mice used as sentinels. The virus is able ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.103627

    authors： Matos GC,Ferreira MS,Martins Filho AJ,Amador Neto OP,Campos VM,Lima MLG,Rodrigues JCP,Ribeiro ACDS,Freitas MNO,Silva FA,Chiang JO,Casseb LMN,Sousa JR,Quaresma JAS,Martins LC,Vasconcelos PFDC,Carvalho VL

    更新日期：2019-10-01 00:00:00

  • Occurrence of virulence factors in Cronobacter sakazakii and Cronobacter malonaticus originated from clinical samples.

    abstract：BACKGROUND:Cronobacter spp. are Gram-negative, facultative-anaerobic, non-spore forming, enteric coliform bacteria, which belongs to the Enterobacteriaceae family. Cronobacter spp. are opportunistic pathogens that have brought rare but life-threatening infections such as meningitis, necrotizing enterocolitis and bloods...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.12.011

    authors： Holý O,Cruz-Córdova A,Xicohtencatl-Cortes J,Hochel I,Parra-Flores J,Petrželová J,Fačevicová K,Forsythe S,Alsonosi A

    更新日期：2019-02-01 00:00:00

  • Chlamydia pneumoniae infection in IL-10 knock out mice: accelerated clearance but severe pulmonary inflammatory response.

    abstract：:In interleukin-10 knock out (IL-10 KO) mice, accelerated clearance of pulmonary Chlamydia pneumoniae infection was observed. On the other hand, the histopathological changes in lung tissue were more pronounced in IL-10 KO mice at all time points after infection and repeated infection than in the wild type mice. Both e...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2008.02.004

    authors： Penttilä T,Haveri A,Tammiruusu A,Vuola JM,Lahesmaa R,Puolakkainen M

    更新日期：2008-07-01 00:00:00

  • Cervicovaginal microbiota dysbiosis correlates with HPV persistent infection.

    abstract：:HPV persistent infection is a main event leading to the development of cervical intraepithelial neoplasia and cervical cancer. Earlier to distinguish HPV persistent and transient infection is meaningful but the methods are limited. This study used 16S rDNA sequencing to determine the cervicovaginal microbiota of HPV p...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104617

    authors： Qingqing B,Jie Z,Songben Q,Juan C,Lei Z,Mu X

    更新日期：2020-11-15 00:00:00

  • Presence of F-like OriT base-pair sequence on the virulence plasmids of Salmonella serovars Gallinarum, Enteritidis, and Typhimurium, but absent in those of Choleraesuis and Dublin.

    abstract：:The 90 kb virulence plasmids of Salmonella biosers Gallinarum and Pullorum (expressed as serovar Gallinarum) are non-conjugative. They were, however, found to be readily mobilized by IncFI and IncFIV plasmids, but not by conjugative IncA, IncB = C, IncH, IncL and IncM R plasmids. No virulence plasmids of serovars Chol...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1994.1048

    authors： Ou JT,Lin MY,Chao HL

    更新日期：1994-07-01 00:00:00

  • Deletion and overexpression studies on DacB2, a putative low molecular mass penicillin binding protein from Mycobacterium tuberculosis H(37)Rv.

    abstract：:Mycobacterium tuberculosis genome encodes several high and low molecular mass penicillin binding proteins. One such low molecular mass protein is DacB2 encoded by open reading frame Rv2911 of M. tuberculosis which is predicted to play a role in peptidoglycan synthesis. In this study we have tried to gain an insight in...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2011.11.003

    authors： Bourai N,Jacobs WR Jr,Narayanan S

    更新日期：2012-02-01 00:00:00

  • Reactivity of Haemophilus influenzae type b anti-pili antibodies.

    abstract：:The reactivity of anti-pilus antibodies to native and denatured Haemophilus influenzae b (Hib) pili was studied using rabbit serum prepared against piliated H. influenzae b strain M43 (p+) and adsorbed with its non-piliated variant, strain M42 (p-). The specificity of the adsorbed serum for Hib pili was documented by ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(89)90049-1

    authors： Gilsdorf JR,Forney LJ,LiPuma JJ

    更新日期：1989-10-01 00:00:00

  • Plant growth promoting and antifungal asset of indigenous rhizobacteria secluded from saffron (Crocus sativus L.) rhizosphere.

    abstract：:Saffron (Crocus sativus L.) is an important plant in medicine. The Kashmir Valley (J&K, India) is one of the world's largest and finest saffron producing regions. However, over the past decade, there has been a strong declining trend in saffron production in this area. Plant Growth Promoting Rhizobacteria (PGPR) are f...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2021.104734

    authors： Rasool A,Imran Mir M,Zulfajri M,Hanafiah MM,Azeem Unnisa S,Mahboob M

    更新日期：2021-01-08 00:00:00

  • Characterization of a DNA region containing 5'-(CAAT)(n)-3' DNA sequences involved in lipooligosaccharide biosynthesis in Haemophilus somnus.

    abstract：:Repetitive tetranucleotide sequences of 5'-(CAAT)(n)-3' have been identified at the 5' end of an open reading frame (ORF) named lob1 from Haemophilus somnus strain 738. Based on sequence analysis, lob1 has 59% DNA homology to lex2B, which is involved in lipooligosaccharide (LOS) biosynthesis in H. influenzae. We now r...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1999.0351

    authors： McQuiston JH,McQuiston JR,Cox AD,Wu Y,Boyle SM,Inzana TJ

    更新日期：2000-05-01 00:00:00

  • Characterization of non-aflatoxigenic strains of Aspergillus flavus as potential biocontrol agent for the management of aflatoxin contamination in groundnut.

    abstract：:In the present study, nine non-aflatoxigenic Aspergillus flavus strains were evaluated for their potential to reduce A. flavus infection and aflatoxin contamination in groundnut. Genetic characterization of these strains revealed six different deletion patterns (A-F) for thirteen examined genes from the aflatoxin bios...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2016.11.007

    authors： Hulikunte Mallikarjunaiah N,Jayapala N,Puttaswamy H,Siddapura Ramachandrappa N

    更新日期：2017-01-01 00:00:00

  • Alteration of gene expression in human middle ear epithelial cells induced by influenza A virus and its implication for the pathogenesis of otitis media.

    abstract：:Influenza A virus infection plays a significant role in the pathogenesis of Streptococcus pneumoniae-induced acute otitis media in children. An understanding of how influenza A virus modulates host cellular responses is critically important in efforts to explore the molecular mechanisms of this synergism. We used micr...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2004.06.012

    authors： Tong HH,Long JP,Li D,DeMaria TF

    更新日期：2004-10-01 00:00:00

  • Expression and sequence diversity of the complement regulating outer surface protein E in Borrelia afzelii vs. Borrelia garinii in patients with erythema migrans or neuroborreliosis.

    abstract：:Outer surface protein E (OspE) is a complement factor H-binding virulence factor of borrelial subspecies. It is usually absent from in vitro grown Borrelia garinii, although in vivo B. garinii causes neuroborreliosis (NB). We analyzed the presence and sequence spectrum of the ospE genes in vivo in Borrelia spirochetes...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2010.06.006

    authors： Panelius J,Ranki A,Meri T,Seppälä I,Meri S

    更新日期：2010-12-01 00:00:00

  • Complete genome sequence of Janthinobacterium sp. B9-8, a violacein-producing bacterium isolated from low-temperature sewage.

    abstract：:Janthinobacterium sp. B9-8, isolated from low temperature-sewage in Xinjiang, China, is capable of producing violacein, a promising antibiotic. Here we report the genome sequence of B9-8, which consist of 4,726,850 bp with a G + C content of 48.72%. The violacein biosynthesis gene cluster vioABCDE was identified and a...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.01.003

    authors： Xu X,Tian L,Zhang S,Jiang L,Zhang Z,Huang H

    更新日期：2019-03-01 00:00:00

  • Need for TolC, an Escherichia coli outer membrane protein, in the secretion of heat-stable enterotoxin I across the outer membrane.

    abstract：:Escherichia coli heat-stable enterotoxin Ip (STIp) is a typical extracellular toxin consisting of 18 amino acid residues synthesized as a precursor of pre (amino acid residues 1 to 19), pro (amino acid residues 20 to 54), and mature (amino acid residues 55 to 72) regions. STIp synthesized in the cytoplasm must cross t...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1998.0211

    authors： Yamanaka H,Nomura T,Fujii Y,Okamoto K

    更新日期：1998-09-01 00:00:00

  • Enhanced production of α-amylase by Penicillium chrysogenum in liquid culture by modifying the process parameters.

    abstract：:In this paper, we have assessed the role of changing physicochemical parameters and substrate types on the production of α-amylase enzyme from Penicillium chrysogenum, with a view to determining the optimal conditions required for its maximum production. The findings of this research revealed that, at pH 6 using linse...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2015.07.016

    authors： Dar GH,Kamili AN,Nazir R,Bandh SA,Jan TR,Chishti MZ

    更新日期：2015-11-01 00:00:00

  • Assessment of antigenic supplementation of bovine purified protein derivative for diagnosis of subclinical infection with Mycobacterium bovis in cattle.

    abstract：:Bovine tuberculosis (bTB) is usually diagnosed in vivo and ex vivo on the basis of delayed hypersensitivity reactions with a complex pool of antigens named bovine tuberculin (PPDB). The IFN-γ release assay (IGRA) for bTB is a blood-based assay that improves detection of infected cattle at early stages that escape skin...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.05.012

    authors： Alvarez AH,Gutiérrez-Ortega A,Gómez-Entzin V,Pérez-Mayorga G,Naranjo-Bastién J,González-Martínez V,Milián-Suazo F,Martínez-Velázquez M,Herrera-Rodríguez S,Hinojoza-Loza E

    更新日期：2017-07-01 00:00:00

  • Inhibition of TLR8 mediated signaling promotes BCG induced apoptosis in THP-1 cells.

    abstract：:Apoptosis was considered as one of the important host defense mechanisms against mycobacteria infection. In macrophage, the main target cell of Mycobacterium tuberculosis, apoptosis after infection could help kill the bacillus inside and process the antigens for further presentation and proper immune response. Here, w...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2015.11.028

    authors： Tang J,Zhan L,Qin C

    更新日期：2016-04-01 00:00:00

  • Inflammatory response induced by Helicobacter pylori infection in lung.

    abstract：:Helicobacter pylori is a microorganism that in the last years has been associated with extragastric disorders such as respiratory diseases, however, its impact on lung is partially understood. The aim of this work was to study infection impact of H. pylori on the inflammatory markers expression at the pulmonary level ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104103

    authors： Arismendi Sosa AC,Salinas Ibáñez AG,Pérez Chaca MV,Penissi AB,Gómez NN,Vega AE

    更新日期：2020-02-26 00:00:00

  • Mutation of csk, encoding the C-terminal Src kinase, reduces Pseudomonas aeruginosa internalization by mammalian cells and enhances bacterial cytotoxicity.

    abstract：:Clinical isolates of Pseudomonas aeruginosa are either invasive or cytotoxic towards mammalian epithelial cells, endothelial cells, and macrophages. Invasion requires host cell actin cytoskeleton function, and ExsA-regulated proteins of P. aeruginosa that inhibit invasion (ExoS and ExoT) can disrupt the cytoskeleton. ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.2002.0521

    authors： Evans DJ,Kuo TC,Kwong M,Van R,Fleiszig SM

    更新日期：2002-09-01 00:00:00

  • Alveolar microbiota profile in patients with human pulmonary tuberculosis and interstitial pneumonia.

    abstract：BACKGROUND:The presence of the human lung microbiota has been demonstrated in patients with different lung diseases, mainly in sputum samples. However, for study of the alveolar microbiota, a bronchoalveolar lavage (BAL) sample represents the lower respiratory tract (LRT) environment. It is currently unknown whether th...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.103851

    authors： Vázquez-Pérez JA,Carrillo CO,Iñiguez-García MA,Romero-Espinoza I,Márquez-García JE,Falcón LI,Torres M,Herrera MT

    更新日期：2020-02-01 00:00:00

  • Protective immunity in mouse salmonellosis: comparison of smooth and rough live and killed vaccines.

    abstract：:Protective immunity against Salmonella infection was studied in a mouse model. To study specificity of protection we used smooth (O-4,5,12) and rough vaccines; live and killed vaccines of both types were compared. The protection was assessed by enumerating the number of bacteria in the livers, and by following surviva...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(89)90007-7

    authors： Muotiala A,Hovi M,Mäkelä PH

    更新日期：1989-01-01 00:00:00

  • Is Toxoplasma gondii a potential risk factor for Alzheimer's disease? A systematic review and meta-analysis.

    abstract：:Toxoplasmosis is a major public health concern due to neurotropic nature and role in the development of mental and behavioral disorders. Alzheimer's disease (AD) is an important nervous disease that results in the reduction of the amount of beta-amyloid plaque deposition and irreversible loss of neurons in the brain. ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.micpath.2019.103751

    authors： Nayeri Chegeni T,Sarvi S,Moosazadeh M,Sharif M,Aghayan SA,Amouei A,Hosseininejad Z,Daryani A

    更新日期：2019-12-01 00:00:00

  • Molecular prevalence of putative virulence-associated genes in Brucella melitensis and Brucella abortus isolates from human and livestock specimens in Iran.

    abstract：:Molecular prevalence of nine putative virulence factors in two more prevalent Brucella species in Iranian patients and livestock was investigated. During five years (2010-2015), 120 human and animal specimens were collected from three geographical areas of Iran. All samples were cultured in blood culture media and sub...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.03.007

    authors： Hashemifar I,Yadegar A,Jazi FM,Amirmozafari N

    更新日期：2017-04-01 00:00:00

  • Chlamydia pneumoniae facilitates monocyte adhesion to endothelial and smooth muscle cells.

    abstract：:Chlamydia pneumoniae has been linked to atherosclerotic heart disease. However, there is a limited knowledge by which C. pneumoniae gain access to atheromatous lesions. The adhesion of C. pneumoniae -infected circulatory component(s) to endothelium and smooth muscle cells represents the first step in an inflammatory r...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.2000.0420

    authors： Kaul R,Wenman WM

    更新日期：2001-03-01 00:00:00

  • Zero valent silver nanoparticles capped with capsaicinoids containing Capsicum annuum extract, exert potent anti-biofilm effect on food borne pathogen Staphylococcus aureus and curtail planktonic growth on a zebrafish infection model.

    abstract：:Food plants Hungarian wax pepper (HWP) and Green Bell pepper (GBP), belonging to Capsicum annuum were utilized for biogenic fabrication of zero valent, nano-silver (AgNPs) through a photo-mediation procedure. In the bacterial strains evaluated, HWP/GBP AgNPs demonstrated effective bacteriostatic and bactericidal effec...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.08.053

    authors： Lotha R,Shamprasad BR,Sundaramoorthy NS,Ganapathy R,Nagarajan S,Sivasubramanian A

    更新日期：2018-11-01 00:00:00

  • Changes in ovine erythrocyte morphology due to sphingomyelin degradation by Corynebacterium pseudotuberculosis phospholipase D.

    abstract：:Corynebacterium pseudotuberculosis produces a sphingomyelin-specific phospholipase D exotoxin that is a major determinant in the pathogenesis of caseous lymphadenitis. We assessed the changes induced in the morphology and sphingomyelin concentration of ovine erythrocytes after incubation with broth culture filtrates o...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(90)90080-a

    authors： Brogden KA,Engen RL,Songer JG,Gallagher J

    更新日期：1990-02-01 00:00:00

  • The effect of improved formulation of chitosan-alginate microcapsules of Bifidobacteria on serum lipid profiles in mice.

    abstract：BACKGROUND:Probiotics have been associated with many beneficial effects in human digestive physiology. The aim of this study was to evaluate the effect of improved formulation of chitosan-alginate microcapsules of Bifidobacterium strains on serum triglycerides, cholesterol, HDL, and LDL in mice. METHODS:Five approved ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104585

    authors： Lohrasbi V,Abdi M,Asadi A,Rohani M,Esghaei M,Talebi M,Amirmozafari N

    更新日期：2020-12-01 00:00:00

  • Systematic expression profiling analysis mines dys-regulated modules in active tuberculosis based on re-weighted protein-protein interaction network and attract algorithm.

    abstract：:About 90% of tuberculosis (TB) patients latently infected with Mycobacterium tuberculosis (Mtb) show no symptoms, yet have a 10% chance in lifetime to progress active TB. Nevertheless, current diagnosis approaches need improvement in efficiency and sensitivity. The objective of this work was to detect potential signat...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.03.013

    authors： Sun Y,Weng Y,Zhang Y,Yan X,Guo L,Wang J,Song X,Yuan Y,Chang FY,Wang CL

    更新日期：2017-06-01 00:00:00

  • Immunopathogenesis of infection with the visceralizing Leishmania species.

    abstract：:Human leishmaniasis is a spectral disease that includes asymptomatic self-resolving infection, localized skin lesions, and progressive visceral leishmaniasis. With some overlap, visceral and cutaneous leishmaniasis are usually caused by different species of Leishmania. This review focuses on host responses to infectio...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,评审

    doi：10.1016/j.micpath.2004.11.002

    authors： Wilson ME,Jeronimo SM,Pearson RD

    更新日期：2005-04-01 00:00:00