Abstract：
:Carbohydrate analyses of whole-spore extracts have confirmed the presence of rhamnose in the spore of the fully virulent Ames strain of Bacillus anthracis. A gene cluster containing loci with high homology to the rhamnose biosynthetic genes, rmlACBD, was identified within the B. anthracis chromosome. The first gene of this cluster, rmlA, was inactivated by forming a merodiploid cointegrate using an internal fragment of the gene within the Ames strain of B. anthracis to construct the mutant strain Ames-JAB1. Carbohydrate analysis of spores from this mutant demonstrated the loss of rhamnose. When assaying for spore infection of macrophages, we detected a significant decrease in the recovery with the Ames-JAB1 strain compared to the recovery with the Ames wild-type strain. When pre-treating macrophages with cytochalasin-D, spores of the mutant were further hindered in recovery, indicating that the spores were not able to bind as well to the macrophages. However, in guinea pigs challenge experiments, no difference in virulence was observed between the mutant and wild-type strains. These results suggest that the incorporation of rhamnose into the spore coat of B. anthracis is required for optimal interaction with macrophages but is not required for full virulence in this animal model.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Bozue JA,Parthasarathy N,Phillips LR,Cote CK,Fellows PF,Mendelson I,Shafferman A,Friedlander AMdoi
10.1016/j.micpath.2004.10.001subject
Has Abstractpub_date
2005-01-01 00:00:00pages
1-12issue
1eissn
0882-4010issn
1096-1208pii
S0882-4010(04)00143-3journal_volume
38pub_type
杂志文章abstract：:Lipopolysaccharide (LPS) is a major virulence factor of Gram-negative bacteria playing a major role in stimulating protective immune response in mammalian host. However, in many gram-negative bacterial infections, LPS also elicits immunopathology by inducing excessive inflammatory changes. P. multocida (Pm), a gram-ne...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.04.030
更新日期：2018-06-01 00:00:00
abstract：:Along with robust immunogenicity, an ideal vaccine candidate should be able to produce a long lasting protection. In this regard, the frequency of memory B-cells is possibly an important factor in memory B-cell persistency and duration of immunological memory. On this basis, binding domains of tetanus toxin (HcT), bot...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.12.003
更新日期：2019-02-01 00:00:00
abstract：:At present, there is not any available accepted vaccine for prevention of Toxoplasma gondii (T. gondii) in human and animals. We conducted literature search through English (Google Scholar, PubMed, Science Direct, Scopus, EBSCO, ISI Web of Science) scientific paper databases to find the best vaccine candidates against...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.11.003
更新日期：2019-01-01 00:00:00
abstract：:Intravenous inoculation of Swiss mice with S. aureus leads to severe synovial joint tissue swelling along with prominent T lymphocyte infiltrate with associated inflammation in synovial tissue. Cytokines released from macrophages such as TNF-α, IL-1β and IL-6 the main players that precede cartilage and bone destructio...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.10.033
更新日期：2017-12-01 00:00:00
abstract：:Iron is an environmental signal which regulates the coordinate expression of genes associated with virulence in many pathogenic bacteria. In response to iron-deprivation, lysogenic Corynebacterium diphtheriae C7 (beta) synthesizes and secretes diphtheria toxin and siderophore and induces a high-affinity iron uptake sy...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(90)90015-i
更新日期：1990-10-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Bacterial vaginosis (BV) is the most common vaginal infection in women of reproductive age. It shifts the paradigms of the vagina from healthy, beneficial microbiota to facultative and strict anaerobes. BV remains one of the most arduous and controversial challenges in modern-day clinical micr...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104588
更新日期：2020-12-01 00:00:00
abstract：:Thanks to the progress and decreasing costs in genome sequencing technologies, more than 250,000 bacterial genomes are currently available in public databases, covering most, if not all, of the major human-associated phylogenetic groups of these microorganisms, pathogenic or not. In addition, for many of them, sequenc...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104275
更新日期：2020-12-01 00:00:00
abstract：:Salmonella serovars were examined for the presence of fim gene sequences using specific DNA probes. All strains, regardless of their ability to express surface-associated fimbriae, retain a considerable amount of DNA homologous to the gene probes used. The phenotypically nonfimbriae FIRN and non-FIRN strains of S. typ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(91)90115-q
更新日期：1991-06-01 00:00:00
abstract：:The gene (ystB) encoding the novel subtype of the heat-stable enterotoxin (Y-STb) was cloned from the chromosome of a clinical isolate of Yersinia enterocolitica 84-50 (serotype O:5, biotype 1A) and the nucleotide sequence was determined. The ystB contained 216 base pairs that encoded a protein of 71 amino acid residu...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1997.0146
更新日期：1997-10-01 00:00:00
abstract：:A total of 36 Escherichia coli urinary tract isolates (UTI) of serotype O6, with different combinations of capsule (K) and flagellin (H) antigens, were analysed according to the outer membrane pattern (OMP), serum resistance properties, mannose-resistant hemagglutination using various types of erythrocytes, and also f...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(92)90048-s
更新日期：1992-04-01 00:00:00
abstract：:The oligopeptide permease (Opp) cassette, an oligopeptide transport system belongs to the superfamily of ATP-binding cassette (ABC) transporter, is widely distributed in bacteria, including Streptococcus suis (S. suis). It is encoded by the opp operon containing oppA, oppB, oppC, oppD, and oppF. In addition to the upt...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.03.061
更新日期：2018-05-01 00:00:00
abstract：:Brucellosis, a disease caused by the gram-negative bacterium Brucella spp., is a widespread zoonosis that inflicts important animal and human health problems, especially in developing countries. One of the hallmarks of Brucella infection is its capacity to establish a chronic infection, characteristic that depends on ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2014.01.003
更新日期：2014-02-01 00:00:00
abstract：:Actinobacillus pleuropneumoniae is the pathogen of porcine contagious pleuropneumonia causing great economic losses globally. LuxS is known to be involved in the interspecies quorum sensing by mediating the production of AI-2 signal molecule and play important roles in regulating various behaviors of bacteria. In this...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2008.05.008
更新日期：2008-09-01 00:00:00
abstract：:The aim of this study is to evaluate antidiarrheal activity of SKB_Gutbiotic against Castor oil and E.coli induced diarrhea in Swiss albino mice and Sprague Dawley rats. In present study three doses of SKB_Gutbiotic were tested against castor oil induced diarrhea in mice. Its effect on co-administration with l-arginin...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104078
更新日期：2020-06-01 00:00:00
abstract：:The emergence of extensively drug-resistant (XDR) Acinetobacter baumannii strains and the limited number of efficacious antibiotics demonstrate an urgent need to develop novel agents to treat infections caused by this dangerous pathogen. To find antimicrobial peptides against A. baumannii growing either in planktonic ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.05.051
更新日期：2018-08-01 00:00:00
abstract：:Bovine mastitis is one of the most costly and prevalent disease affecting dairy cows worldwide. It was reported that Staphylococcus aureus could internalize into bovine mammary epithelial cells (bMEC) and induce mastitis. Some short chain fatty acids (SCFA) have shown to suppress S. aureus invasion into bMEC and regul...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.03.030
更新日期：2017-06-01 00:00:00
abstract：:In Egypt, although the Trypanosoma evansi has been reported frequently among domestic animals, there is no published data on T. evansi in horses. Therefore, this study aimed to assess the prevalence and characterization of T. evansi in three governorates by examining blood samples from 40 local camels, 35 imported cam...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.07.017
更新日期：2018-10-01 00:00:00
abstract：:Natural habitats of opportunistic fungal pathogens are outside of the host; therefore, it is critically important to understand their ecology and routes of transmission. In this study, we investigated the presence of human pathogenic opportunistic fungi in lake water and incidence of fungal infections in associated po...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2016.01.022
更新日期：2016-04-01 00:00:00
abstract：:Protein E (PE) is a conserved entity observed in both nontypeable Haemophilus influenzae (NTHi) and encapsulated H. influenzae. This is a small surface lipoprotein, consisting of only 160 amino acids, involved in the adhesion of H. influenzae to various types of epithelial cells. A 384-bp-long fragment from NTHi PE wa...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.07.025
更新日期：2017-09-01 00:00:00
abstract：:Host cell invasion is important for periodontal pathogens in evading host defenses and spreading into deeper areas of the periodontal tissue. Treponema denticola has been implicated in a number of potentially pathogenic processes, including periodontal tissue penetration. Here we tested the ability of T. denticola str...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2016.01.010
更新日期：2016-05-01 00:00:00
abstract：OBJECTIVE:DNA vaccine has emerged as a promising approach with potential for Tuberculosis (TB) prevention in adults. However, the mechanism behind DNA vaccines is still largely unknown. MATERIALS AND METHODS:Utilizing the CRISPR/Cas9 technique, we engineered Ag85A mutated dendritic cells (Ag85A-M-DCs) in which the Ag8...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104487
更新日期：2020-12-01 00:00:00
abstract：:Monocyte chemoattractant protein-1 (MCP-1), a chemoattractant for monocytes, is presumed to play a pivotal role in the recruitment and accumulation of monocytes in various diseases including pulmonary infections. We examined here whether or not Pseudomonas nitrite reductase (PNR), a recently identified IL-8 inducer in...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1999.0320
更新日期：2000-01-01 00:00:00
abstract：INTRODUCTION:Tuberculosis (TB) is considered as a serious complication for organ transplant recipients; therefore, the detection and appropriate treatment of latent infection are recommended for preventing active TB infections in the future. The objective of this review is to conduct a systematic review and meta-analys...
journal_title：Microbial pathogenesis
pub_type： 杂志文章,meta分析
doi：10.1016/j.micpath.2018.09.040
更新日期：2018-12-01 00:00:00
abstract：:Recent studies based on sequence divergence in the ospC gene have identified limited subpopulations of B. burgdorferi associated with invasive human disease. Spirochetes with certain OspC types never cause human disease, while some others cause local infection at the primary skin site but do not hematogenously dissemi...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/s0882-4010(03)00152-9
更新日期：2003-12-01 00:00:00
abstract：:The effect of recombinant Brugia malayi pepsin inhibitor (rBm33) on human monocytes/macrophages has been examined using THP-1 cells. THP-1 cells stimulated with rBm33 showed enhanced levels of expression of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6) and diminished levels of IL-12, iNOS and anti-inflammatory cytok...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2012.03.009
更新日期：2012-07-01 00:00:00
abstract：:The 190 kDa outer membrane protein of Rickettsia rickettsii is a major immunodominant protective antigen which contains 13 tandem nearly identical repeating sequences. We have identified a deletion in the 190 kDa antigen gene of R. rickettsii strain Smith maintained in the laboratory. The deletion occurred within the ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1996.0005
更新日期：1996-01-01 00:00:00
abstract：:Poultry meat is a major vector for Campylobacter jejuni foodborne illness. Since C. jejuni is microaerophillic, the cell counts gradually decreased during distribution and display under aerobic condition. However, if the pathogen can resist to aerobic condition, it may cause serious problem in food safety. This study ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.05.020
更新日期：2019-09-01 00:00:00
abstract：:Enteropathogenic strains of Yersinia enterocolitica harbor a virulence plasmid (pYVe plasmid) of 70 kilobases (kb) which specifies, at 37 degrees C, a calcium requirement for growth, autoagglutinability, resistance to the bactericidal activity of human serum, and the expression of outer membrane proteins (OMP). Some m...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(86)90067-7
更新日期：1986-08-01 00:00:00
abstract：:Good vaccines should confer protection against specific pathogens in experimental and field conditions. However, some commercial vaccines are not capable to confer protection to animals, being inefficient in a bovine vaccine program. In this sense, the aim of this study was to evaluate the antibody levels involved in ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.10.042
更新日期：2018-01-01 00:00:00
abstract：:Pneumocystis infection leads to a life threatening pneumonia in susceptible individuals. While depletion or dysfunction of CD4+T cells is a key determinant of susceptibility to Pneumocystis, the host response that leads to resolution of infection or lung injury is less well understood. We had previously shown that mic...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2005.10.001
更新日期：2006-01-01 00:00:00
