当前位置： SCI文献检索 > MICROBIAL PATHOGENESIS期刊下所有文献 > Treponema denticola invasion into human gingival epithelial cells.

Treponema denticola invasion into human gingival epithelial cells.

Abstract：

:Host cell invasion is important for periodontal pathogens in evading host defenses and spreading into deeper areas of the periodontal tissue. Treponema denticola has been implicated in a number of potentially pathogenic processes, including periodontal tissue penetration. Here we tested the ability of T. denticola strains to invade human gingival epithelial cells (HGEC). After 2 h infection, intracellular location of T. denticola cells was confirmed by confocal laser scanning microscopy (CLSM). Results from an antibiotic protection assay following [(3)H]uridine labeling indicated that invasion efficiency reached a maximum at 2 h after infection. Internalized T. denticola cells were still observed in HGEC at 24 h by CLSM. A dentilisin deficient mutant exhibited significantly decreased invasion (p < 0.05) compared with the wild-type strain. In inhibition assays, phenylmethylsulfonyl fluoride and metabolic inhibitors such as methyl-β-cyclodextrin and staurosporine significantly reduced T. denticola invasion. Under CLSM, T. denticola colocalized with GM-1 ganglioside-containing membrane microdomains in a cholesterol-dependent manner. These results indicated that T. denticola has the ability to invade into and survive within HGECs. Dentilisin activity of T. denticola and lipid rafts on HGEC appear to play important roles in this process.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Inagaki S,Kimizuka R,Kokubu E,Saito A,Ishihara K

doi

10.1016/j.micpath.2016.01.010

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

104-11

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(16)00008-5

journal_volume

94

pub_type

杂志文章

相关文献

MICROBIAL PATHOGENESIS文献大全
  • Detection of feline haemoplasma species in experimental infections by in-situ hybridisation.

    abstract：:The aim of this study was to use fluorescence in-situ hybridisation (FISH) to search for the tissues and cell types important in survival and persistence of Mycoplasma haemofelis, "Candidatus Mycoplasma haemominutum" or "Candidatus Mycoplasma turicensis" in infected cats. A 16S rDNA probe for each species was applied ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2010.09.003

    authors： Peters IR,Helps CR,Willi B,Hofmann-Lehmann R,Gruffydd-Jones TJ,Day MJ,Tasker S

    更新日期：2011-02-01 00:00:00

  • Antibacterial studies of hydroxyspiro[indoline-3,9-xanthene]trione against spiro[indoline3,9-xanthene]trione and their use as acetyl and butyrylcholinesterase inhibitors.

    abstract：:Xanthene derivatives are well known for their effective biological activities. In search of effective antibacterial agents, the spiro[indoline3,9-xanthene]-trione (A) and hydroxy-spiro[indoline-3,9-xanthene]-trione (B), were synthesized and tested for in vitro antibacterial activity against Staphylococcus aureus and E...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.03.002

    authors： Barmak A,Niknam K,Mohebbi G,Pournabi H

    更新日期：2019-05-01 00:00:00

  • The role of O1-antigen in the adhesion to uroepithelial cells of Klebsiella pneumoniae grown in urine.

    abstract：:We obtained mutants devoid of the O1-antigen, the capsular polysaccharide (K antigen) or both from Klebsiella pneumoniae clinical isolates (urinary infection). These mutants were grown in urine, and their ability to fimbriate and to adhere were studied. Mutants lacking the O1-antigen, independently of the other surfac...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1997.0132

    authors： Merino S,Rubires X,Aguilar A,Tomás JM

    更新日期：1997-07-01 00:00:00

  • In vitro studies of epithelium-associated crystallization caused by uropathogens during urinary calculi development.

    abstract：:Infectious urinary stones account for about 10% of all urinary stones. In 50% of cases urolithiasis is a recurrent illness, which can lead to the loss of a kidney if not properly treated. One of the reasons for recurrence of the disease may be the ability of bacteria to invade urothelial cells, persist in the host cel...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2014.04.007

    authors： Torzewska A,Budzyńska A,Białczak-Kokot M,Różalski A

    更新日期：2014-06-01 00:00:00

  • Antibacterial and antibiofilm activities of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) against periodontopathic bacteria.

    abstract：:Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are two major omega-3 polyunsaturated fatty acids (n-3 PUFAs) with antimicrobial properties. In this study, we evaluated the potential antibacterial and antibiofilm activities of DHA and EPA against two periodontal pathogens, Porphyromonas gingivalis (P. gingi...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2016.08.025

    authors： Sun M,Zhou Z,Dong J,Zhang J,Xia Y,Shu R

    更新日期：2016-10-01 00:00:00

  • Elevated neutrophil, macrophage and dendritic cell numbers characterize immune cell populations in mice chronically infected with Salmonella.

    abstract：:The present study characterizes immune cell populations in mice chronically infected with Salmonella. Mice were characterized as chronically infected based on persistently high titers of Salmonella-reactive immunoglobulins in the serum >6 months after a single oral dose of S. enterica serovar Typhimurium. These mice h...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2006.03.004

    authors： Johansson C,Ingman M,Jo Wick M

    更新日期：2006-08-01 00:00:00

  • Lack of association between hemolysin production and acute inflammation in human urinary tract infection.

    abstract：:Hemolysins are cytolytic proteins which have been extensively characterized at the molecular level, however, their in vivo functions remain unclear. This study analyzed the association of hemolysin production with the inflammatory response in patients with urinary tract infection (UTI). Infants and children with their...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1993.1045

    authors： Connell H,de Man P,Jodal U,Lincoln K,Svanborg C

    更新日期：1993-06-01 00:00:00

  • Comprehensive analysis of the phylogeny and extracellular proteases in genus Vibrio strain.

    abstract：:As one of the dominant bacteria in the ocean, Vibrio play important roles in maintaining the aquatic ecosystem. In this study, we studied the phylogenetic relationships of 32 Vibrio based on the 16S rRNA genes sequences and utilized substrate immersing zymography method to detect the trend of protease production and c...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.03.018

    authors： Liu D,Wu C,Wu R,Huang J,Liao B,Lei M,Zhang Y,He H

    更新日期：2019-06-01 00:00:00

  • Venom-derived peptide Mastoparan-1 eradicates planktonic and biofilm-embedded methicillin-resistant Staphylococcus aureus isolates.

    abstract：:During the past decade, cationic antimicrobial peptides (CAPs) have gained particular interest among researchers, since they often display broad-spectrum antimicrobial activity and low possibility of resistance emergence. This study aimed to investigate in vitro effectiveness of Mastoparan-1 (MP-1), a tetradecapeptide...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.04.008

    authors： Memariani H,Memariani M,Pourmand MR

    更新日期：2018-06-01 00:00:00

  • Comparison of the pulmonary response against lethal and non-lethal intranasal challenges with two different pneumococcal strains.

    abstract：:The differences between the immune response elicited during a self-limiting and a life-threatening lung infection with Streptococcus pneumoniae was analyzed in a mouse model of intranasal challenge using two different pneumococcal strains. M10, a serotype 11A strain, induced an early response within the first 12h afte...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2009.05.005

    authors： Ferreira DM,Moreno AT,Cianciarullo AM,Ho PL,Oliveira ML,Miyaji EN

    更新日期：2009-09-01 00:00:00

  • Hyperexpression of type III secretion system of Salmonella Typhi linked to a higher cytotoxic effect to monocyte-derived macrophages by activating inflammasome.

    abstract：:Inflammasome activation is an important host response to infectious diseases, but the difference in inflammasome activation between typhoid fever and non-typhoidal Salmonella infection has been rarely studied. To determine whether inflammasome activation in macrophages after S. Typhi and S. Typhimurium infection is di...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104222

    authors： Lin HH,Chen HL,Janapatla RP,Chen CL,Chiu CH

    更新日期：2020-09-01 00:00:00

  • Enterococcus faecalis internalization in human umbilical vein endothelial cells (HUVEC).

    abstract：:Initial Enterococcus faecalis-endothelial cell molecular interactions which lead to enterococci associating in the host endothelial tissue, colonizing it and proliferating there can be assessed using in vitro models. Cultured human umbilical vein endothelial cells (HUVEC) have been used to study other Gram-positive ba...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2012.11.007

    authors： Millán D,Chiriboga C,Patarroyo MA,Fontanilla MR

    更新日期：2013-04-01 00:00:00

  • Differences in biofilm formation and virulence factors between clinical and fecal enterococcal isolates of human and animal origin.

    abstract：:The present study investigated the possible correlation between carriage of the virulence genes esp and fsrb, production of hemolysin and gelatinase and biofilm formation in human vs. animal enterococcal isolates. A collection of 219 enterococcal isolates recovered from clinical and fecal surveillance samples of hospi...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2012.03.003

    authors： Tsikrikonis G,Maniatis AN,Labrou M,Ntokou E,Michail G,Daponte A,Stathopoulos C,Tsakris A,Pournaras S

    更新日期：2012-06-01 00:00:00

  • Treatment with 3'-deoxyadenosine and deoxycoformycin in mice infected by Trypanosoma cruzi and its side effect on purinergic enzymes.

    abstract：:The aim of this study was to evaluate the efficacy of 3'-deoxyadenosine and deoxycoformycin combination in the treatment of mice infected by T. cruzi, as well as to verify the influence of the treatment on purinergic enzymes. Heart and serum samples were collected from 60 mice (30 infected and 30 uninfected) at day 12...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.10.030

    authors： do Carmo GM,Doleski PH,de Sá MF,Grando TH,Azevedo MI,Manzoni AG,Leal DBR,Gressler LT,Henker LC,Mendes RE,Baldissera MD,Monteiro SG,Stefani LM,Da Silva AS

    更新日期：2017-12-01 00:00:00

  • Dendrimer-enabled transformation of Chlamydia trachomatis.

    abstract：:Lack of a system for genetic manipulation of Chlamydia trachomatis has been a key challenge to advancing understanding the molecular genetic basis of virulence for this bacterial pathogen. We developed a non-viral, dendrimer-enabled system for transformation of this organism and used it to characterize the effects of ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2013.08.003

    authors： Kannan RM,Gérard HC,Mishra MK,Mao G,Wang S,Hali M,Whittum-Hudson JA,Hudson AP

    更新日期：2013-12-01 00:00:00

  • Alteration of gene expression in human middle ear epithelial cells induced by influenza A virus and its implication for the pathogenesis of otitis media.

    abstract：:Influenza A virus infection plays a significant role in the pathogenesis of Streptococcus pneumoniae-induced acute otitis media in children. An understanding of how influenza A virus modulates host cellular responses is critically important in efforts to explore the molecular mechanisms of this synergism. We used micr...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2004.06.012

    authors： Tong HH,Long JP,Li D,DeMaria TF

    更新日期：2004-10-01 00:00:00

  • GroEL PCR- RFLP - An efficient tool to discriminate closely related pathogenic Vibrio species.

    abstract：:Vibrio sp. are autochthonous to marine and estuarine waters. Several species of Vibrio are pathogens. It is of utmost importance to detect and discriminate the Vibrio sp. that are often involved in food and water borne infections. Since 16S rRNA based identification has limited utility in differentiating the closely r...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.02.029

    authors： Silvester R,Alexander D,Antony AC,Hatha M

    更新日期：2017-04-01 00:00:00

  • Effect of immunosuppression on pathogenesis of peste des petits ruminants (PPR) virus infection in goats.

    abstract：:In this study an attempt to address the effects of immunosuppression on pathogenesis of peste des petits ruminants (PPR) virus infection was undertaken. Cyclophosphamide and dexamethasone were used to immunosuppress the animals. The drug treated animals exhibited severe leukopaenia and lymphopaenia; one of the indicat...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2012.01.003

    authors： Jagtap SP,Rajak KK,Garg UK,Sen A,Bhanuprakash V,Sudhakar SB,Balamurugan V,Patel A,Ahuja A,Singh RK,Vanamayya PR

    更新日期：2012-04-01 00:00:00

  • Bioinformatics of excretory/secretory proteins of Toxoplasma gondii strain ME49.

    abstract：:Toxoplasma gondii is an obligate intracellular parasite that is globally distributed and can infect almost all warm-blooded animals, including humans. While vaccines are not available for prophylaxis, there are limited therapeutic options which often do not result in eradication of parasites from patients. ME49 is a c...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.103951

    authors： Ma Z,Mutashar Alhameed AM,Kaminga AC,Lu B,Li X,Zhang J,Wu X

    更新日期：2020-03-01 00:00:00

  • Quantification of Staphylococcus aureus cell surface adhesins using flow cytometry.

    abstract：:The initiation of many infectious diseases involves specific adhesion of bacteria to host tissue proteins and carbohydrates. Staphylococcus aureus is known to bind specifically to several proteins in the extracellular matrix (ECM). We report the quantification of the collagen and fibronectin adhesin densities on the s...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.2000.0399

    authors： Mohamed N,Visai L,Speziale P,Ross JM

    更新日期：2000-12-01 00:00:00

  • Analyzing the adhesion mechanism of FnBPA, a surface adhesin from Staphylococcus aureus on its interaction with nanoparticle.

    abstract：:Staphylococcus aureus expresses many Microbial Surface Recognizing Adhesive Matrix Molecules (MSCRAMM's) to recognize host extracellular matrix (ECM) molecules to initiate colonization. The MSCRAMM, fibronectin binding protein A (FnBPA), is an important adhesin for S. aureus infection. FnBPA also binds with fibrinogen...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104239

    authors： Ponnuvel S,Sankar S,Ponnuraj K

    更新日期：2020-09-01 00:00:00

  • Virtual screening of plant compounds and nonsteroidal anti-inflammatory drugs for inhibition of quorum sensing and biofilm formation in Salmonella.

    abstract：:Salmonella belongs to the Enterobacteriaceae family which is widely distributed in the environment due to its adaptive capacity to stress conditions. In addition, Salmonella is able to perform a type of cell-to-cell communication called quorum sensing, which leads to differential gene expression. The quorum sensing sy...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.05.014

    authors： Almeida FA,Vargas ELG,Carneiro DG,Pinto UM,Vanetti MCD

    更新日期：2018-08-01 00:00:00

  • Anti-quorum sensing and antibiofilm activities of Blastobotrys parvus PPR3 against Pseudomonas aeruginosa PAO1.

    abstract：:The bacterial cell communication also termed as Quorum sensing (QS) system was involved in the expression of several virulence traits during Pseudomonas infection. The attenuating of this bacterial cell communication system is an attractive approach for the management of bacterial infections without the complication o...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.103811

    authors： Parasuraman P,Devadatha B,Sarma VV,Ranganathan S,Ampasala DR,Siddhardha B

    更新日期：2020-01-01 00:00:00

  • Immune response of a commercial vaccine against Leptospira interrogans: Antibodies and cytokine levels.

    abstract：:Good vaccines should confer protection against specific pathogens in experimental and field conditions. However, some commercial vaccines are not capable to confer protection to animals, being inefficient in a bovine vaccine program. In this sense, the aim of this study was to evaluate the antibody levels involved in ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.10.042

    authors： Fávero JF,Fritzen A,Lovato LT,Martins P,Baldissera MD,Stefani LM,Da Silva AS

    更新日期：2018-01-01 00:00:00

  • Discrimination between apo and iron-loaded forms of transferrin by transferrin binding protein B and its N-terminal subfragment.

    abstract：:Many pathogens of the Pasteurellaceae and Neisseriaceae possess a surface receptor that binds transferrin (Tf) as an initial step in an iron acquisition process. This receptor is comprised of two proteins, transferrin binding protein A (TbpA) and transferrin binding protein B (TbpB). Since the ability to recognize the...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1998.0226

    authors： Retzer MD,Yu Rh,Zhang Y,Gonzalez GC,Schryvers AB

    更新日期：1998-10-01 00:00:00

  • Experimental infection of golden hamsters with Guama virus (Peribunyaviridae, Orthobunyavirus).

    abstract：:The Guama virus (GMAV) is a member of Peribunyaviridae family, Orthobunyavirus genus. Several strains of the virus were isolated in South and Central Americas from several hosts, such as humans, wild animals, including nonhuman primates, wild rodents and mosquitoes as well as mice used as sentinels. The virus is able ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.103627

    authors： Matos GC,Ferreira MS,Martins Filho AJ,Amador Neto OP,Campos VM,Lima MLG,Rodrigues JCP,Ribeiro ACDS,Freitas MNO,Silva FA,Chiang JO,Casseb LMN,Sousa JR,Quaresma JAS,Martins LC,Vasconcelos PFDC,Carvalho VL

    更新日期：2019-10-01 00:00:00

  • Genome-wide identification of genes regulated by RcsA, RcsB, and RcsAB phosphorelay regulators in Klebsiella pneumoniae NTUH-K2044.

    abstract：:Rcs phosphorelay system is a two-component signal transduction system, which can regulate the transcription of capsule polysaccharide and biofilm related genes in Enterobacteriaceae. In this study, microarray technology was used to investigate the overall genes regulated by RcsA, RcsB, and RcsAB and the regulation mec...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.06.036

    authors： Su K,Zhou X,Luo M,Xu X,Liu P,Li X,Xue J,Chen S,Xu W,Li Y,Qiu J

    更新日期：2018-10-01 00:00:00

  • Salmonella Typhimurium induces cloacitis-like symptomsin zebrafish larvae.

    abstract：:Pathogenic Salmonella strains have a set of virulence factors allowing them to generate systemic infections and damage in a variety of hosts. Among these factors, bacterial proteins secreted by specialized systems are used to penetrate the host's intestinal mucosa, through the invasion and destruction of specialized e...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.04.010

    authors： Varas M,Ortíz-Severín J,Marcoleta AE,Díaz-Pascual F,Allende ML,Santiviago CA,Chávez FP

    更新日期：2017-06-01 00:00:00

  • The 95ΔG mutation in the 5'untranslated region of the norA gene increases efflux activity in Staphylococcus epidermidis isolates.

    abstract：:In the Staphylococcus aureus ATCC25923 strain, the flqB mutation in the 5'untranslated region (5'UTR) of the norA gene causes increased norA mRNA expression and high efflux activity (HEA). The involvement of the norA gene 5'UTR in HEA has not been explored in S. epidermidis; therefore, we examined the function of this...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2016.12.021

    authors： García-Gómez E,Jaso-Vera ME,Juárez-Verdayes MA,Alcántar-Curiel MD,Zenteno JC,Betanzos-Cabrera G,Peralta H,Rodríguez-Martínez S,Cancino-Díaz ME,Jan-Roblero J,Cancino-Diaz JC

    更新日期：2017-02-01 00:00:00

  • Impaired resistance to infection does not increase the virulence of Salmonella htrA live vaccines for mice.

    abstract：:We have described a new class of live attenuated salmonella vaccines harbouring lesions in htrA, a stress protein gene previously. The virulence and invasiveness of Salmonella htrA mutants was investigated in three models of increased susceptibility to Salmonella infection. These included BALB/c mice, either given sub...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(92)90049-t

    authors： Strahan K,Chatfield SN,Tite J,Dougan G,Hormaeche CE

    更新日期：1992-04-01 00:00:00