Abstract：
:During the past decade, cationic antimicrobial peptides (CAPs) have gained particular interest among researchers, since they often display broad-spectrum antimicrobial activity and low possibility of resistance emergence. This study aimed to investigate in vitro effectiveness of Mastoparan-1 (MP-1), a tetradecapeptide CAP from hornet venom, against methicillin-resistant Staphylococcus aureus (MRSA) isolates. MP-1 had a high propensity to form alpha-helix based on structural predictions. MP-1 was found to possess strong antimicrobial activities and weak cytotoxic effects. Multiple treatments of MRSA with MP-1 at sub-lethal dose did not induce resistance. At 4 × minimum bactericidal concentration (MBC), MP-1 eradicated bacteria within 60 min, whereas vancomycin was unable to eradicate MRSA even after 480 min of exposure, highlighting rapid bactericidal kinetics of MP-1. Treatment of bacteria with 2 × MBC of MP-1 caused a time-dependent increase in orange/red fluorescence intensity. Compared with vancomycin, MP-1 significantly reduced biofilm formation and diminished both biofilm biomass and viability of biofilm-embedded bacteria in a concentration-dependent manner. Taken together, the current data reveal not only that MP-1 is a potent bactericidal and antibiofilm agent, but also that it is less likely to invoke antimicrobial resistance, reinforcing further studies concerning the therapeutic applications of MP-1.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Memariani H,Memariani M,Pourmand MRdoi
10.1016/j.micpath.2018.04.008subject
Has Abstractpub_date
2018-06-01 00:00:00pages
72-80eissn
0882-4010issn
1096-1208pii
S0882-4010(17)31713-8journal_volume
119pub_type
杂志文章abstract：:Enterococci are opportunistic pathogens known to cause numerous clinical infections and complications in humans. Adhesin-mediated binding to extracellular matrix (ECM) proteins of the host is thought to be a crucial step in the pathogenesis of these bacterial infections. Adhesin of collagen from Enterococcus faecalis ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2007.03.003
更新日期：2007-08-01 00:00:00
abstract：:ADP-ribosylating protein exotoxins from Vibrio cholerae (CT) and Escherichia coli (LT-I) share two short regions of sequence similarity with Bordetella pertussis toxin (PT). Previous studies have indicated that substitution of arginine for lysine 7 within the first region of CT drastically decreases ADP ribosyltransfe...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1994.1079
更新日期：1994-11-01 00:00:00
abstract：:The 90 kb virulence plasmids of Salmonella biosers Gallinarum and Pullorum (expressed as serovar Gallinarum) are non-conjugative. They were, however, found to be readily mobilized by IncFI and IncFIV plasmids, but not by conjugative IncA, IncB = C, IncH, IncL and IncM R plasmids. No virulence plasmids of serovars Chol...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1994.1048
更新日期：1994-07-01 00:00:00
abstract：:Inflammatory cytokines have been described to play a critical role in the orientation and amplification of the IgA immune response. In this study, we show that the intranasal administration of a Bordetella pertussis strain expressing the protective antigen glutathione-S-transferase of Schistosoma mansoni (Sm28GST) ind...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1996.0130
更新日期：1997-05-01 00:00:00
abstract：:Pneumocystis infection leads to a life threatening pneumonia in susceptible individuals. While depletion or dysfunction of CD4+T cells is a key determinant of susceptibility to Pneumocystis, the host response that leads to resolution of infection or lung injury is less well understood. We had previously shown that mic...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2005.10.001
更新日期：2006-01-01 00:00:00
abstract：:Mycoplasma pneumoniae (M. pneumoniae) is a small bacterium characterized by the absence of cell wall. It is a human pathogen causing upper and lower respiratory infections, both in adults and children. However, it is also considered to be implicated in the pathogenesis of several types of extra-respiratory diseases, i...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103863
更新日期：2020-02-01 00:00:00
abstract：:Rhoptry proteins (ROPs) play a significant role in various stages of Toxoplasma gondii (T. gondii) life cycle, being critical for both invasion and intracellular survival. ROP38 is a key manipulator of host gene expression and has a function in tachyzoite to bradyzoite conversion. In this study, we've employed various...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104488
更新日期：2020-12-01 00:00:00
abstract：:The ica operon and aap gene are important factors for Staphylococcus epidermidis biofilm formation. However, we found 15 out of 101 S. epidermidis strains isolated from patients had both the ica operon and the aap gene in the genome but could not form biofilms (ica(+)aap(+)/BF(-) isolates). Compared with standard stra...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2014.09.013
更新日期：2014-11-01 00:00:00
abstract：:A total of 54 positive blood aerobic cultures of suspected blood sepsis in neonates were purified, diagnosed and tested for antibiotic susceptibility. Six bacterial pathogens of Escherichia coli (10 isolates), Klebsiella pneumoniae (10 isolates), Haemophilus influenzae (4 isolates), Pseudomonas aeruginosa (3 isolates)...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104447
更新日期：2020-11-01 00:00:00
abstract：:Toxoplasmosis is a major public health concern due to neurotropic nature and role in the development of mental and behavioral disorders. Alzheimer's disease (AD) is an important nervous disease that results in the reduction of the amount of beta-amyloid plaque deposition and irreversible loss of neurons in the brain. ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章,meta分析
doi：10.1016/j.micpath.2019.103751
更新日期：2019-12-01 00:00:00
abstract：:To assess the role of mannosylated lipoarabinomannan (ManLAM) in the inflammatory and apoptotic response of mycobacteria-infected and uninfected, bystander cells we applied a mouse macrophage model of infection with avirulent strains--Mycobacterium bovis BCG, Mycobacterium tuberculosis (MTB) H37Ra and compared with a ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2011.03.004
更新日期：2011-07-01 00:00:00
abstract：:The enteropathogenic potential of 32 Vibrio cholerae O1 isolates that do not produce cholera toxin was examined in the orally inoculated, sealed adult mouse model. Live cultures (2 x 10(10) cfu/ml) of 7/16 clinical and 6/16 environmental isolates produced a positive intestinal fluid accumulation (FA) ratio that reache...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(92)90008-c
更新日期：1992-06-01 00:00:00
abstract：:The extracellular hemolysin (AHH1) gene of Aeromonas hydrophila ATCC7966 was cloned into Charomid9-28 in Escherichia coli DH1, and its complete nucleotide sequence determined. Escherichia coli carrying this gene expressed an extracellular heat-labile hemolysin for rabbit red blood cells. The minimum size of the coding...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(91)90049-g
更新日期：1991-09-01 00:00:00
abstract：:New strategies are required to improve the efficacy of drugs and to treat the emerging microbial resistance. An effective strategy is to combine drugs with metal nanoparticles for the control of microbial infections and resistance. Keeping in view this fact, we developed a facile and eco-friendly protocol for the synt...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2016.08.031
更新日期：2016-10-01 00:00:00
abstract：:Ehrlichia risticii, the causative agent of Potomac horse fever, is an obligate intracellular rickettsial organism. To understand the role of 55 and 51 kilodalton immunodominant antigens of E. risticii in strain variation, their genes from the 25-D and 90-12 strains were cloned, sequenced, and expressed in E. coli. Seq...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1998.0208
更新日期：1998-06-01 00:00:00
abstract：BACKGROUND:Tuberculosis (TB) remains a major public health problem. SH3RF1 and SH3RF2 are candidate genes with multiple single-nucleotide polymorphisms (SNPs) that have the potential to participate in Mycobacterium infection via activation of the JNK signaling pathway. In this case-control study, we aimed to investigat...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104567
更新日期：2020-10-28 00:00:00
abstract：:Streptokinase (SK), a heterogeneous plasminogen (Pg) activator protein secreted by groups A, C and G streptococci (GAS/GCS/GGS) is a virulence factor composed of three structural domains; SKα/SKβ/SKγ. Phylogenetic analysis of the major variable region of SKβ (sk-V1; nucleotides 448-791; 343bp) which classifies the SK ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103862
更新日期：2020-02-01 00:00:00
abstract：:Fimbrial adhesins and hemolysins contribute to pathogenicity of extraintestinal Escherichia coli isolates causing urinary tract infections (UTI), sepsis and new born meningitis (NBM). Using gene cloning techniques and pulse field electrophoresis in combination with Southern hybridizations it was demonstrated that the ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(90)90048-u
更新日期：1990-03-01 00:00:00
abstract：:The involvement of nitric oxide (NO) in host defense and cytoprotective functions in murine salmonellosis has been reported. Salmonella mutants with the altered sigma factor RpoS (sigmaS) are less virulent and are susceptible to various stresses. This study investigated the role of the rpoS gene of Salmonella enterica...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2005.11.007
更新日期：2006-03-01 00:00:00
abstract：:Meningococci can be classified by the presence of serotype and subtype antigens on the class 2/3 and class 1 outer membrane proteins, respectively. A typing system employing monoclonal antibodies and a WCE for detection of such antigens enabled us to sero- and/or subtype 255 out of 268 group B meningococci from 19 cou...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(88)90045-9
更新日期：1988-01-01 00:00:00
abstract：:Anthrax lethal factor (LF), a Zn(2+)-dependent metalloprotease, is a key virulence component of anthrax toxin. Here, we used proteolytic assay-based screening to identify novel LF inhibitors from a naturally extracted chemical library. The screening identified four compounds that inhibited in vitro proteolytic activit...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2012.04.004
更新日期：2012-08-01 00:00:00
abstract：:In this study, the effects of water, ethanol, methanol and glycerin at five levels (0, 31.25, 83.33, 125 and 250 ml) were investigated on the efficiency of mangrove leaf extraction using mixture optimal design. The antimicrobial effect of the extracts on Streptococcus pneumoniae, Enterococcus faecium and Klebsiella pn...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.12.004
更新日期：2018-01-01 00:00:00
abstract：:Staphylococcus aureus expresses many Microbial Surface Recognizing Adhesive Matrix Molecules (MSCRAMM's) to recognize host extracellular matrix (ECM) molecules to initiate colonization. The MSCRAMM, fibronectin binding protein A (FnBPA), is an important adhesin for S. aureus infection. FnBPA also binds with fibrinogen...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104239
更新日期：2020-09-01 00:00:00
abstract：:Histophilus somni (H. somni) is a gram-negative bacterial pathogen that causes respiratory, reproductive, and central nervous system disease in cattle. The hallmark of systemic H. somni infection is diffused vasculitis that can lead to an acute central nervous system disease known as thrombotic meningoencephalitis (TM...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2009.06.001
更新日期：2009-09-01 00:00:00
abstract：:At present, there is not any available accepted vaccine for prevention of Toxoplasma gondii (T. gondii) in human and animals. We conducted literature search through English (Google Scholar, PubMed, Science Direct, Scopus, EBSCO, ISI Web of Science) scientific paper databases to find the best vaccine candidates against...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.11.003
更新日期：2019-01-01 00:00:00
abstract：:Edwardsiella tarda poses a threat to human health and has resulted in enormous economic losses in aquaculture. Low temperatures are usually applied to contain the growth of this microorganism. In this study, stable isotope labelling by amino acids in cell culture (SILAC) was used to conduct comparative proteomic quant...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.09.006
更新日期：2018-12-01 00:00:00
abstract：:The emergence of extensive antibiotics resistant bacteria increased the demands for finding out new sources of antimicrobial agents. Marine niches were reported to be rich in many competent producers of significant bioactive compounds. On the course of screening program for new antimicrobials, a Bacillus strain was is...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.08.014
更新日期：2018-11-01 00:00:00
abstract：:Molecular-genetic properties of classical biotype Vibrio cholerae strains that caused the Asiatic cholera outbreak in 1942 in Russia have been investigated for the first time. Being characterized by high-level production of cholera toxin and toxin-coregulated adhesion pili both of which are the major virulence factors...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2003.10.004
更新日期：2004-03-01 00:00:00
abstract：BACKGROUND:Since the pre-transplant status affects the renal transplantation success and ultimately the survival rate, identifying the probable risk factors that increase the chance of BK virus replication in end-stage renal disease patients can be included in proposing proper surveillance guidelines during pre and pos...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104558
更新日期：2020-12-01 00:00:00
abstract：:The first occurrences and dissemination of resistant microorganisms led to the inefficacy of many antibiotics, available in the market nowadays, therefore, the search for new substances with antimicrobial activity from natural sources has gained a great importance. The purpose of this work is to evaluate the antibacte...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.01.028
更新日期：2017-03-01 00:00:00
