Expression and mutagenesis of recombinant cholera toxin A subunit.

abstract：AIMS:The main objective of the present study was to assess and compare the safety and inhibitory efficacy of Lactobacillus acidophilus against cholera toxin and shigatoxin production by measuring CTX-B and Stx1 expression level in Caco-2 cells exposed to Vibrio cholerae (as a non-invasive small intestine pathogens and ...

journal_title：Microbial pathogenesis

authors： Alamdary SZ,Bakhshi B

更新日期：2020-12-01 00:00:00

abstract：:Endometritis is commonly occurred in dairy cows after calving and results in a great deal of property damage. Although numerous studies have been performed to find the therapeutic agents for endometritis, the incidence of this disease remains high. Short-chain fatty acids (SCFAs), the major metabolic products of anaer...

journal_title：Microbial pathogenesis

authors： Guo J,Wang Y,Jiang P,Yao H,Zhao C,Hu X,Cao Y,Zhang N,Fu Y,Shen H

更新日期：2019-12-01 00:00:00

abstract：:Acinetobacter baumannii potential to form biofilm and exhibit multiple antibiotic resistances may be responsible in its survival in hospital environment. Accordingly, our study was aimed to determine the correlation between ability of biofilm formation and the frequency of biofilm related genes with antibiotic resista...

journal_title：Microbial pathogenesis

authors： Bardbari AM,Arabestani MR,Karami M,Keramat F,Alikhani MY,Bagheri KP

更新日期：2017-07-01 00:00:00

abstract：:In the present work two key regulator proteins, monomeric MipZ of Caulobacter vibrioides (similar to Pseudomonas aeruginosa) and Pyruvate kinase of Staphylococcus aureus were docked with curcumin, the wonder molecule from the spice turmeric and structures of its twelve analogues were designed, synthesized and tested i...

journal_title：Microbial pathogenesis

authors： Shrivash MK,Mishra S,UpmaNarain,Pandey J,Misra K

更新日期：2018-10-01 00:00:00

abstract：:In this study we generated and characterized a series of monoclonal antibodies (mAbs) against GII.6 norovirus (NoV) virus like particles (VLPs). Mice were immunized with purified GII.6 NoV VLPs and peptide-bovine serum albumin (BSA) conjugates with the peptide sequence (31 aa) derived from the trypsin cleavage region....

journal_title：Microbial pathogenesis

authors： Qiu S,Zheng L,Qin C,Yin X,Ma J,Liu J,Yang Z,Li C,Wang Y,Wang M,Qi Y,Huo Y

更新日期：2020-02-26 00:00:00

abstract：:TLR4 is a membrane sensor for lipopolysaccharide (LPS), a major cell wall component of gram-negative bacteria. In this study, we investigated the role of TLR4 on innate immune responses in immune cells against Acinetobacter baumannii. Bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) were isolated fr...

journal_title：Microbial pathogenesis

authors： Kim CH,Jeong YJ,Lee J,Jeon SJ,Park SR,Kang MJ,Park JH,Park JH

更新日期：2013-01-01 00:00:00

abstract：:Escherichia coli heat-stable enterotoxin Ip (STIp) is a typical extracellular toxin consisting of 18 amino acid residues synthesized as a precursor of pre (amino acid residues 1 to 19), pro (amino acid residues 20 to 54), and mature (amino acid residues 55 to 72) regions. STIp synthesized in the cytoplasm must cross t...

journal_title：Microbial pathogenesis

authors： Yamanaka H,Nomura T,Fujii Y,Okamoto K

更新日期：1998-09-01 00:00:00

abstract：:The closely related Shiga toxins, Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2), can bind to Gb3 receptors. However, Stx2-producing enterohemorrhagic Escherichia coli (EHEC) strains are more commonly associated with serious human disease (viz., hemolytic-uremic syndrome) than Stx1-producing strains. To clarify the rel...

journal_title：Microbial pathogenesis

authors： Shimizu T,Sato T,Kawakami S,Ohta T,Noda M,Hamabata T

更新日期：2007-08-01 00:00:00

abstract：:Acute lung injury (ALI) is considered as an uncontrolled inflammatory response that can leads to acute respiratory distress syndrome (ARDS), which limits the therapeutic strategies. Ginsenosides Rb1 (Rb1), an active ingredient obtained from Panax ginseng, possesses a broad range of pharmacological and medicinal proper...

journal_title：Microbial pathogenesis

authors： Shaukat A,Yang C,Yang Y,Guo YF,Jiang K,Guo S,Liu J,Zhang T,Zhao G,Ma X,Wu Z,Zhou Q,Akhtar M,Zahoor A,Umar T,Shaukat I,Hanif S,Rajput SA,Hassan M,Mehmood K,Hua Z,Xiaoyan W,Nannan Y,Deng G

更新日期：2020-06-01 00:00:00

abstract：:NorA, NorB, and NorC are efflux proteins in the Nor family that regulate the secretion of fluoroquinolones, and MgrA/NorR is a transcription factor of the Nor family. Overexpression of Nor family proteins provides fluoroquinolone resistance in Staphylococcus aureus. However, in coagulase-negative staphylococci (CNS), ...

journal_title：Microbial pathogenesis

authors： Juárez-Verdayes MA,Parra-Ortega B,Hernández-Rodríguez C,Betanzos-Cabrera G,Rodríguez-Martínez S,Cancino-Diaz ME,Cancino-Diaz JC

更新日期：2012-06-01 00:00:00

abstract：:Bacterial extracellular vesicles (EVs) have come forth into notice as possible important agent to mediate host-pathogen interactions. In this scientific research, the authors have tried to find out the effect of EVs derived from Lactobacillus rhamnosus GG (LDEVs) on the apoptosis induction in HepG2 cell line. The EVs ...

journal_title：Microbial pathogenesis

authors： Behzadi E,Mahmoodzadeh Hosseini H,Imani Fooladi AA

更新日期：2017-09-01 00:00:00

abstract：:Two highly passaged laboratory strains of human cytomegalovirus (HCMV), AD169 and Towne, were tested for their ability to infect and replicate in THP-1 myelomonocytic cells differentiated with 12-O-tetradecanoylphorbol-13-acetate (TPA). TPA treatment of human THP-1 cells increased the number of cells that expressed HC...

journal_title：Microbial pathogenesis

authors： Turtinen LW,Seufzer BJ

更新日期：1994-05-01 00:00:00

abstract：:A trans-acting, positive effector of invasion plasmid antigen (Ipa) synthesis has been identified and mapped on the pWR100 invasion plasmid of Shigella flexneri serotype 5 (strain M90T-W). Recombinant plasmids carrying this regulatory gene, designated ipaR, were found to restore full virulence to a non-invasive ipaR::...

journal_title：Microbial pathogenesis

authors： Buysse JM,Venkatesan M,Mills JA,Oaks EV

更新日期：1990-03-01 00:00:00

abstract：:Multidrug-resistant Acinetobacter baumannii isolates cause critical problems in health-care environments. AdeABC is a resistance-nodulation-cell division (RND)-type multidrug efflux pump conferring resistance to clinically essential antibiotics in A. baumannii, such as ciprofloxacin. This study aimed to target adeB ge...

journal_title：Microbial pathogenesis

authors： Abdi SN,Ghotaslou R,Asgharzadeh M,Mehramouz B,Hasani A,Baghi HB,Tanomand A,Narenji H,Yousefi B,Gholizadeh P,Yousefi M,Bastami M,Ganbarov K,Samadi Kafil H

更新日期：2020-02-01 00:00:00

abstract：:The accumulation of inflammatory cells in the peritoneal cavity of C57BL/6 mice was examined following intraperitoneal injection of Entamoeba histolytica trophozoites. Two different strains of E. histolytica were used: a virulent strain (IP:0682:1) and a non-virulent strain (DKB). Injection of 10(6) trophozoites of ei...

journal_title：Microbial pathogenesis

authors： Ghadirian E,Stevenson MM

更新日期：1987-09-01 00:00:00

abstract：:Indole is a metabolite of tryptophan that can be synthesized by various bacteria. In the present study, production of indole by Vibrio splendidus Vs was determined using Kovac's reagent, and m/z was further determined by HPLC-MS. Extracellular indole reached a maximum concentration of 160 μM, when OD600 of V. splendid...

journal_title：Microbial pathogenesis

authors： Zhang S,Zhang W,Liu N,Song T,Liu H,Zhao X,Xu W,Li C

更新日期：2017-10-01 00:00:00

abstract：:The re-emerging field of phage therapy is the potential biocontrol agents for the transfer of virulence factor and to kill their bacterial hosts. In this study, the lytic Vibrio vulnificus phages were studied to provide a better understanding of phage-host interactions and development of phage therapy. Four new V. vul...

journal_title：Microbial pathogenesis

authors： Srinivasan P,Ramasamy P

更新日期：2017-10-01 00:00:00

abstract：:Escherichia coli heat-stable enterotoxin b (STb) causes severe diarrhoea in weaning piglets. STb most probably has to bind to intestinal epithelial cells in order to achieve its effect. Using biotinylated biologically active STb, we developed a semi-quantitative binding assay using indirect fluorescence microscopy. We...

journal_title：Microbial pathogenesis

authors： Rousset E,Harel J,Dubreuil JD

更新日期：1998-05-01 00:00:00

abstract：:Today, there is an increasing emphasis on recombinant vaccines to eliminate the side effects of conventional vaccines such as whole-cell bacteria. Query fever is an emerging disease that causes irreparable complications for both humans and domestic animals. The cause of this disease is Coxiella burnetii, a gram-negati...

journal_title：Microbial pathogenesis

authors： Jaydari A,Forouharmehr A,Nazifi N

更新日期：2019-01-01 00:00:00

abstract：:Mycobacterium ulcerans, a decaying Mycobacterium marinum derivative is responsible for Buruli ulcer, a notifiable non-contagious disabling infection highly prevalent in some West African countries. Aquatic environments are suspected to host M. ulcerans, however, the exact reservoirs remain unknown. While M. marinum wa...

journal_title：Microbial pathogenesis

authors： Bouam A,Ghigo E,Drancourt M

更新日期：2018-01-01 00:00:00

abstract：:Protein D, having a glycerol-3-phosphodiester phosphodiesterase activity, is found at the surface of all Haemophilus influenzae strains and is a possible virulence factor. In the present study, the involvement of protein D in the entry of NTHi into human monocytic cells is reported. Primary monocytes and the monocytic...

journal_title：Microbial pathogenesis

authors： Ahrén IL,Janson H,Forsgren A,Riesbeck K

更新日期：2001-09-01 00:00:00

abstract：:Carbohydrate analyses of whole-spore extracts have confirmed the presence of rhamnose in the spore of the fully virulent Ames strain of Bacillus anthracis. A gene cluster containing loci with high homology to the rhamnose biosynthetic genes, rmlACBD, was identified within the B. anthracis chromosome. The first gene of...

journal_title：Microbial pathogenesis

authors： Bozue JA,Parthasarathy N,Phillips LR,Cote CK,Fellows PF,Mendelson I,Shafferman A,Friedlander AM

更新日期：2005-01-01 00:00:00

abstract：:We aimed to identify key pathways to further explore the molecular mechanism underlying pediatric pneumonia using differential pathway network which integrated protein-protein interactions (PPI) data and pathway information. PPI data and pathway information were obtained from STRING and Reactome database, respectively...

journal_title：Microbial pathogenesis

authors： Yang JB,Luo R,Yan Y,Chen Y

更新日期：2016-12-01 00:00:00

abstract：:Vibrio cholerae elaborates zonula occludens toxin (Zot), a protein that increases the permeability of small intestinal mucosa by opening intercellular tight junctions. The zot gene is located, together with the genes encoding CT and Ace enterotoxins, within the genome of V. cholerae filamentous phage CTXsmall ef, Cyri...

journal_title：Microbial pathogenesis

authors： Uzzau S,Cappuccinelli P,Fasano A

更新日期：1999-12-01 00:00:00

abstract：:In this study an attempt to address the effects of immunosuppression on pathogenesis of peste des petits ruminants (PPR) virus infection was undertaken. Cyclophosphamide and dexamethasone were used to immunosuppress the animals. The drug treated animals exhibited severe leukopaenia and lymphopaenia; one of the indicat...

journal_title：Microbial pathogenesis

authors： Jagtap SP,Rajak KK,Garg UK,Sen A,Bhanuprakash V,Sudhakar SB,Balamurugan V,Patel A,Ahuja A,Singh RK,Vanamayya PR

更新日期：2012-04-01 00:00:00

abstract：:Crohn's disease (CD) is characterized by a pathogenic intestinal inflammation mediated by a Th1-skewed immune system and a dysregulated intestinal mucosal community. In this study, we investigated the role of bacteria on the activation and function of monocytes, and its association with CD pathogenesis. To this end, f...

journal_title：Microbial pathogenesis

authors： Gu J,Liu G,Xing J,Song H,Wang Z

更新日期：2018-08-01 00:00:00

abstract：:The high incidence of bacterial respiratory infections has led to a focus on evaluating the human respiratory microbiome. Studies based on culture-based and molecular methods have shown an increase in the bacterial community that includes the bacterial phyla Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteri...

journal_title：Microbial pathogenesis

authors： Maleki A,Zamirnasta M,Taherikalani M,Pakzad I,Mohammadi J,Krutova M,Kouhsari E,Sadeghifard N

更新日期：2020-06-01 00:00:00

abstract：:The oral cavity is regarded as a relevant site for Staphylococcus aureus colonization. However, characterization of virulence mechanisms of oral S. aureus remains to be uncovered. In this study, twenty one S. aureus strains isolated from the oral cavity of Tunisian patients were screened for adherence, invasion and cy...

journal_title：Microbial pathogenesis

authors： Merghni A,Ben Nejma M,Helali I,Hentati H,Bongiovanni A,Lafont F,Aouni M,Mastouri M

更新日期：2015-09-01 00:00:00

abstract：:An inbred strain of the southern platyfish, Xiphophorus maculatus, was used as a host for Aeromonas hydrophila and Yersinia ruckeri infections. The infections were initiated by holding the platyfish in inoculation baths containing dilutions of virulent A. hydrophila or Y. ruckeri strains. Inoculating the platyfish in ...

journal_title：Microbial pathogenesis

authors： Kawula TH,Lelivelt MJ,Orndorff PE

更新日期：1996-02-01 00:00:00

abstract：:The genus Aeromonas comprises more than thirty Gram-negative bacterial species which mostly act as opportunistic microorganisms. These bacteria are distributed naturally in diverse aquatic ecosystems, where they are easily isolated from animals such as fish and crustaceans. A capacity for adaptation also makes Aeromon...

journal_title：Microbial pathogenesis

authors： Gonçalves Pessoa RB,de Oliveira WF,Marques DSC,Dos Santos Correia MT,de Carvalho EVMM,Coelho LCBB

更新日期：2019-05-01 00:00:00