Abstract：
:A trans-acting, positive effector of invasion plasmid antigen (Ipa) synthesis has been identified and mapped on the pWR100 invasion plasmid of Shigella flexneri serotype 5 (strain M90T-W). Recombinant plasmids carrying this regulatory gene, designated ipaR, were found to restore full virulence to a non-invasive ipaR::Tn5 insertion mutant [M90T-W(pHS1042)] that had lost the ability to synthesize four Ipa antigens (IpaA, 70 kDa; IpaB, 62 kDa; IpaC, 42 kDa; and IpaD, 37 kDa). Genetic mapping of the ipaR gene positioned the locus on a 2.6 kb PstI-AccI fragment contained within a larger 8.0 kb EcoRI molecule that also encoded IpaD, IpaA, and two small proteins (27 kDa and 28 kDa). The trans regulatory effect of the ipaR product on ipaB, ipaC, ipaD, and ipaA expression was demonstrated by transforming compatible ipaBC, ipaDA, ipaR and ipaDAR plasmid recombinants, in various combinations, into M90T-A3, an isogenic invasion plasmid mutant of M90T-W that contained a deletion of the pWR100 ipaBCDA and ipaR loci; such transformants produced wild type levels of the IpaB, IpaC, IpaD and IpaA antigens only in the presence of IpaR+ plasmids. DNA sequence analysis of the ipaR region established that the intiation codon for ipaR is 459 bp from the 3'-end of the ipaA gene and that ipaR encodes a 309 amino acid residue protein. An interesting feature of the IpaR polypeptide was its strong sequence homology with the bacteriophage P1 partition protein ParB, consisting of a 42.8% amino acid identity over a 278 residue section of the aligned proteins.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Buysse JM,Venkatesan M,Mills JA,Oaks EVdoi
10.1016/0882-4010(90)90047-tsubject
Has Abstractpub_date
1990-03-01 00:00:00pages
197-211issue
3eissn
0882-4010issn
1096-1208pii
0882-4010(90)90047-Tjournal_volume
8pub_type
杂志文章abstract：:This research focused on green engineering and characterization of silver (PcAgNPs) and copper nanoparticles (PcCuNPs) using Prosopis cineraria (Pc) leaf extract prepared by using microwave irradiation. We studied their enhanced antimicrobial activity on human pathogens as well as cytotoxicity on breast cancer cells (...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.02.019
更新日期：2017-04-01 00:00:00
abstract：:Heat-stable enterotoxins (ST) activate guanylyl cyclase in T84 cells, rapidly and specifically. Activation is monitored by cGMP production and occurs at lower concentrations of ST than required for eliciting fluid accumulation in suckling mice. Atrial natriuretic factor (ANF) neither activates guanylyl cyclase nor mod...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(92)90055-s
更新日期：1992-03-01 00:00:00
abstract：BACKGROUND:It is generally considered that HLA-Ⅱ genes contribute to the Helicobacter pylori (Hp) infection and disease development process. AIMS:To perform a meta-analysis to explore the relationship between HLA-Ⅱgene polymorphism and host susceptibility to Hp infection. METHODS:Relevant cohort studies, case-control...
journal_title：Microbial pathogenesis
pub_type： 杂志文章,meta分析
doi：10.1016/j.micpath.2015.03.011
更新日期：2015-05-01 00:00:00
abstract：:Three bacterial isolates were identified from infected potato tubers showing soft and blackleg like symptoms as well as one isolate from infected pear tree showing crown gall symptom. Conventional and molecular identification proved that bacterial isolates belonging to Pectobacterium carotovorum subsp. carotovorum, Pe...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.05.032
更新日期：2018-08-01 00:00:00
abstract：:Activation of human gamma delta T cells by culture supernatants of virulent and avirulent mycobacteria was examined. The stimulatory potential of mycobacteria was influenced by the type of culture media and independent from their virulence. Activation of gamma delta T cells by phagocytes infected with viable virulent ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1997.0187
更新日期：1998-03-01 00:00:00
abstract：:In our previous study, complete protection was observed in rabbit immunized with 1 × 1010 CFU of live attenuated VCUSM21P vaccine against challenge with 1 × 109 CFU Vibrio cholerae O139. In the present study, we investigated whether the vaccines can effectively protect immunized animals from any pathologic changes usi...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.103964
更新日期：2020-03-01 00:00:00
abstract：:Salmonella serovars were examined for the presence of fim gene sequences using specific DNA probes. All strains, regardless of their ability to express surface-associated fimbriae, retain a considerable amount of DNA homologous to the gene probes used. The phenotypically nonfimbriae FIRN and non-FIRN strains of S. typ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(91)90115-q
更新日期：1991-06-01 00:00:00
abstract：:Recent advancements highlight the important role of gut microbiome in human health. However, a variety of endogenous and exogenous factors, such as genes, foods, drugs, environmental pollutions, oxidative stress, etc., may interfere with the gut microbiome in vivo and increase risks of digestive system diseases, cardi...
journal_title：Microbial pathogenesis
pub_type： 杂志文章,评审
doi：10.1016/j.micpath.2017.02.024
更新日期：2017-04-01 00:00:00
abstract：BACKGROUND:Bacterial adhesins play an important role in the bacterial attachment and colonization. The aim of this study was comparison of adhesin genes expression in the planktonic and biofilm mode of growth among ESBL-non-producers isolates of K. oxytoca and effect of imipenem. MATERIALS AND METHODS:A total of eight...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103558
更新日期：2019-09-01 00:00:00
abstract：:Mycobacterium tuberculosis genome encodes several high and low molecular mass penicillin binding proteins. One such low molecular mass protein is DacB2 encoded by open reading frame Rv2911 of M. tuberculosis which is predicted to play a role in peptidoglycan synthesis. In this study we have tried to gain an insight in...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2011.11.003
更新日期：2012-02-01 00:00:00
abstract：:Molecular prevalence of nine putative virulence factors in two more prevalent Brucella species in Iranian patients and livestock was investigated. During five years (2010-2015), 120 human and animal specimens were collected from three geographical areas of Iran. All samples were cultured in blood culture media and sub...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.03.007
更新日期：2017-04-01 00:00:00
abstract：:The Aeromonas genus has several virulence factors associated with the development of diseases in aquatic organisms, leading to losses in aquaculture. One of these factors is the flagella's formation which allows the biofilm's formation that provides the microorganisms a greater pathogenicity, greater protection to cer...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.11.044
更新日期：2019-02-01 00:00:00
abstract：:The present study investigated the possible correlation between carriage of the virulence genes esp and fsrb, production of hemolysin and gelatinase and biofilm formation in human vs. animal enterococcal isolates. A collection of 219 enterococcal isolates recovered from clinical and fecal surveillance samples of hospi...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2012.03.003
更新日期：2012-06-01 00:00:00
abstract：:A total of 54 positive blood aerobic cultures of suspected blood sepsis in neonates were purified, diagnosed and tested for antibiotic susceptibility. Six bacterial pathogens of Escherichia coli (10 isolates), Klebsiella pneumoniae (10 isolates), Haemophilus influenzae (4 isolates), Pseudomonas aeruginosa (3 isolates)...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104447
更新日期：2020-11-01 00:00:00
abstract：:The cAMP content of Trypanosoma brucei increases in parallel with ascending mammalian parasitemia to very high levels just before differentiation of the long-slender to the short-stumpy bloodstream form. Because expression of myc oncogenes is required for vertebrate cells to interpret proliferation signals and decline...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(89)90110-1
更新日期：1989-07-01 00:00:00
abstract：:Rcs phosphorelay system is a two-component signal transduction system, which can regulate the transcription of capsule polysaccharide and biofilm related genes in Enterobacteriaceae. In this study, microarray technology was used to investigate the overall genes regulated by RcsA, RcsB, and RcsAB and the regulation mec...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.06.036
更新日期：2018-10-01 00:00:00
abstract：:Diseases caused by bacteria are today one of the great problems of present time. Access to over-the-counter medication, the indiscriminate use of antibacterials and high rates of hospitalization have contributed to the increase in cases. This picture has led to the search for new alternative drugs. Thus, secondary met...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.12.058
更新日期：2018-02-01 00:00:00
abstract：:We previously demonstrated that incubation of E. coli in normal human serum (NHS) resulted in the release of a finite fraction (approximately 30%) of LPS from the bacterial outer membrane. In experiments reported here, we examined factors which may enhance or diminish the capacity of NHS to mediate this limited LPS re...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(88)90068-x
更新日期：1988-03-01 00:00:00
abstract：:Staphylococcus aureus is a bacterial pathogen causing approximately 80% of all cases of human osteomyelitis. This bacterium can adhere to and become internalized by osteoblasts and previous studies indicate that osteoblasts are active in the internalization process. In the current study, we examined the roles of micro...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1999.0272
更新日期：1999-06-01 00:00:00
abstract：:Biosynthesis of nanoparticles by fermented plants using microbes is an eco-friendly and cost-effective process. In this study, we used the fungus Aspergillus orayzae for the fermentation process. The aqueous extract of fermented Lupin (AEFL) possesses the ability to reduce selenium ion in the presence of gamma rays ev...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.06.013
更新日期：2018-09-01 00:00:00
abstract：BACKGROUND:Malaria is a public health concern that leads to about a million deaths worldwide every year. Malaria is caused by the genus Plasmodium, which includes P. falciparum, P. vivax, P. malariae, and P. ovale. Molecular phylogeny is essential to better recognition the evolution of the genus Plasmodium genus and de...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103782
更新日期：2019-12-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2012.10.002
更新日期：2013-03-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(88)90083-6
更新日期：1988-07-01 00:00:00
abstract：:Salmonella pathogenicity island 2 (SPI2) encodes a type III secretion system (TTSS) necessary for bacterial survival and replication in intracellular environment of host cells. SPI2 genes are transcribed preferentially after Salmonella enters the host cells. Transcriptional regulation of ssaG encoding the component of...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2006.03.005
更新日期：2006-07-01 00:00:00
abstract：:This study was design to evaluate the physiological properties of bacteriophage-insensitive Klebsiella pneumoniae (BIKP) mutants in association with the antibiotic cross-resistance, β-lactamase activity, and gene expression. Klebsiella pneumoniae ATCC 23357(KPWT), ciprofloxacin-induced antibiotic-resistant K. pneumoni...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103625
更新日期：2019-10-01 00:00:00
abstract：:The effects of depleting CD4+ and CD8+ T cells on macrophage recruitment have been analyzed for bronchoalveolar lavage (BAL) populations from mice with primary or secondary influenza pneumonia. Macrophages were characterized by both the capacity to engulf latex particles and the expression of mRNA for a 65 kD heat sho...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1993.1008
更新日期：1993-01-01 00:00:00
abstract：:Bacillus anthracis secretes three major components, which assemble into two bipartite toxins: lethal toxin (LT), composed of lethal factor (LF) and protective antigen (PA) and edema toxin (ET), composed of edema factor (EF) and PA. EF is a potent calmodulin-dependent adenylate cyclase, which is internalized into the t...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2011.10.002
更新日期：2012-01-01 00:00:00
abstract：:YopM is a type III secretion effector from Yersinia which contributes to pathogenicity but whose action still remains unclear. It is an acidic, leucine-rich repeats (LRR) containing protein which migrates to the nucleus of target cells in spite of the fact that it does not contain any classical nuclear localization si...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2003.12.006
更新日期：2004-05-01 00:00:00
abstract：:The corticosteroid-treated animal is well established as an experimental model for the study of Pneumocystis carinii pneumonitis (PCP). Latent or acquired infection with P. carinii in the murine lung progresses to fatal pneumonitis when the host is profoundly immunocompromized. In this study the effects of five immuno...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.2000.0374
更新日期：2000-09-01 00:00:00
abstract：OBJECTIVE:Pseudomonas aeruginosa, an opportunistic pathogen, can cause serious health problems and produces several virulence factors. The most important of these factors is biofilm. Many studies suggest administration of new generation of antibiotics, as P. aeruginosa biofilm has developed high resistance to antimicro...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.03.055
更新日期：2018-05-01 00:00:00
