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The interaction of Escherichia coli with normal human serum: factors affecting the capacity of serum to mediate lipopolysaccharide release.

Abstract：

:We previously demonstrated that incubation of E. coli in normal human serum (NHS) resulted in the release of a finite fraction (approximately 30%) of LPS from the bacterial outer membrane. In experiments reported here, we examined factors which may enhance or diminish the capacity of NHS to mediate this limited LPS release. Both the susceptibility to serum killing and LPS release were dependent on growth phase. Optimal killing and release coincided with the midlogarithmic growth phase. The composition of LPS subunits in the outer membrane appeared to influence serum-mediated LPS release. Serum treated E. coli enriched for Rc-chemotype LPS released less LPS from their outer membrane than the wildtype 'smooth' bacteria during exponential growth. LPS fractions released by NHS or EDTA appeared to a large degree to overlap, suggesting that NHS-mediated LPS release may involve the action of a serum chelator. A serum-resistant mutant failed to release LPS in either NHS or EDTA. This latter observation suggests that LPS release may be a relevant event in serum killing. We did not detect any modulation of LPS release when E. coli were pre-incubated with a series of antibiotics prior to treatment with NHS.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Tesh VL,Morrison DC

doi

10.1016/0882-4010(88)90068-x

subject

Has Abstract

pub_date

1988-03-01 00:00:00

pages

175-87

issue

3

eissn

0882-4010

issn

1096-1208

pii

0882-4010(88)90068-X

journal_volume

4

pub_type

杂志文章

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