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Nutritional interference for phenotypic biofilm quantification in Aeromonas spp. isolates containing the fla gene.

Abstract：

:The Aeromonas genus has several virulence factors associated with the development of diseases in aquatic organisms, leading to losses in aquaculture. One of these factors is the flagella's formation which allows the biofilm's formation that provides the microorganisms a greater pathogenicity, greater protection to certain substances such as antibiotics. The aim of the study was to verify the presence of the fla gene, related to biofilm production in isolates of Aeromonas spp. from fishes and also to determine the best quantification condition of phenotypic biofilm production in vitro. Polymerase Chain Reactions were performed to obtain the amplification of the region comprising the fla gene. To determine the best condition for the production biofilm, the microplate adhesion test was carried out under different concentrations of TSB broth and it combined with glucose. Of the 43 isolates of Aeromonas spp. analyzed, 28 were positive for the fla gene and, in the quantification of the biofilm, all these were able to form biofilm in the TSB broth without dilution and without addition of glucose, being this the best condition tested. It was observed that the isolates of Aeromonas spp. analyzed have potential for biofilm formation, and hence potential for virulence.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Freire NB,Magalhães TC,Nunes Soares RA,da Costa MM,Gouveia GV

doi

10.1016/j.micpath.2018.11.044

subject

Has Abstract

pub_date

2019-02-01 00:00:00

pages

198-201

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(17)31462-6

journal_volume

127

pub_type

杂志文章

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