Abstract:
:To determine if RecA plays a role in the virulence of Brucella abortus, a B. abortus RecA mutant was constructed and its survival was examined in mice. The recA gene was cloned from a B. abortus genomic DNA library by complementation of an Escherichia coli recA mutant in the presence of methyl methanesulfonate (MMS). The nucleotide sequence of recA was determined and the deduced protein sequence possesses extensive conservation with other RecA proteins of Gram-negative bacteria. A deletion plasmid was constructed in a suicide vector by deleting a segment of recA and inserting a kanamycin resistance gene. The deletion plasmid was introduced into B. abortus strain 2308, a virulent strain, by electroporation. Replacement of recA with the kanamycin resistance fragment was confirmed by Southern blot analysis. The RecA mutant was more sensitive than the parental strain to killing by MMS. When administered intraperitoneally to BALB/c mice, numbers of bacteria per spleen were consistently lower in animals infected with the RecA mutant than with the parental strain. However, both the RecA mutant and parental strain persisted in mice through 100 days post-infection. These results indicate that RecA is not crucial for persistence of B. abortus in mice.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Tatum FM,Morfitt DC,Halling SMdoi
10.1006/mpat.1993.1018subject
Has Abstractpub_date
1993-03-01 00:00:00pages
177-85issue
3eissn
0882-4010issn
1096-1208pii
S0882-4010(83)71018-1journal_volume
14pub_type
杂志文章abstract::Respiratory infections caused by nontypeable Haemophilus influenzae (NTHi) are a major medical problem. Evidence suggests that the ability to form biofilms on mucosal surfaces may play a role in NTHi pathogenesis. However, the factors that contribute to NTHi biofilm cohesion remain largely unknown. In this study we in...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2009.01.004
更新日期:2009-04-01 00:00:00
abstract:BACKGROUND:The presence of the human lung microbiota has been demonstrated in patients with different lung diseases, mainly in sputum samples. However, for study of the alveolar microbiota, a bronchoalveolar lavage (BAL) sample represents the lower respiratory tract (LRT) environment. It is currently unknown whether th...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.103851
更新日期:2020-02-01 00:00:00
abstract::Endometritis is commonly occurred in dairy cows after calving and results in a great deal of property damage. Although numerous studies have been performed to find the therapeutic agents for endometritis, the incidence of this disease remains high. Short-chain fatty acids (SCFAs), the major metabolic products of anaer...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.103792
更新日期:2019-12-01 00:00:00
abstract::Staphylococcus aureus is one of the major bacterial mastitis pathogens with significant effects on animal and human health. Some studies showed that S. aureus strains that infect different host species are genetically distinct, although most strains can infect a wide range of host species. However, there are no clearl...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104171
更新日期:2020-07-01 00:00:00
abstract::At present, there is not any available accepted vaccine for prevention of Toxoplasma gondii (T. gondii) in human and animals. We conducted literature search through English (Google Scholar, PubMed, Science Direct, Scopus, EBSCO, ISI Web of Science) scientific paper databases to find the best vaccine candidates against...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.11.003
更新日期:2019-01-01 00:00:00
abstract::Anthemis species are traditionally used to treat infectious and inflammatory processes, among others clinical disturbances. In the current study, the chemical composition, the total phenolic and flavonoid contents, the antioxidant, anticholinesterase, antimicrobial, and antibiofilm activities of Anthemis stiparum subs...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.04.033
更新日期:2018-06-01 00:00:00
abstract::The non-fimbrial adhesin NFA-4 from uropathogenic Escherichia coli O7:K98:H6 mediates the agglutination of human red cells (RBC), notably of blood group MM. The adhesin can be separated from the bacteria by heat extraction and was purified to homogeneity by ammonium sulphate precipitation and anion exchange chromatogr...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/0882-4010(89)90077-6
更新日期:1989-05-01 00:00:00
abstract::The changes in the cell surface carbohydrates of the eustachian tube (ET) and middle ear subsequent to the intranasal (i.n.) inoculation of Streptococcus pneumoniae (Spn) type 6A were studied in the chinchilla model of otitis media (OM) using a lectin histochemical technique with six different lectins (SNA, WGA, Succ ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1994.1043
更新日期:1994-06-01 00:00:00
abstract::Fast emerging antibiotic resistance in pathogens requires special attention for strengthening the reservoir of antimicrobial compounds. In view of this, several peptides with known antimicrobial activities have been reported to enhance the efficacy of antibiotics against multidrug resistant (MDR) pathogens. In the pre...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.08.023
更新日期:2018-11-01 00:00:00
abstract::Staphylococcus aureus (S. aureus) is a zoonotic bacterium and is among the most important pathogens causing bacterial foodborne diseases. In recent years, disease caused by antibiotic-resistant S. aureus is a serious clinical problem that poses a great threat to public health. In this study, we examined the drug-resis...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.103549
更新日期:2019-08-01 00:00:00
abstract::A trans-acting, positive effector of invasion plasmid antigen (Ipa) synthesis has been identified and mapped on the pWR100 invasion plasmid of Shigella flexneri serotype 5 (strain M90T-W). Recombinant plasmids carrying this regulatory gene, designated ipaR, were found to restore full virulence to a non-invasive ipaR::...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/0882-4010(90)90047-t
更新日期:1990-03-01 00:00:00
abstract::Establishment of pulmonary Mycobacterium tuberculosis infection requires evasion of host innate defenses. In the lung alveoli, epithelial cells naturally resist uptake by the inhaled bacilli while macrophages patrol the epithelial surface and phagocytose foreign microbes. Alveolar microvascular endothelial cells, howe...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2006.05.002
更新日期:2006-08-01 00:00:00
abstract::Rhoptry proteins (ROPs) play a significant role in various stages of Toxoplasma gondii (T. gondii) life cycle, being critical for both invasion and intracellular survival. ROP38 is a key manipulator of host gene expression and has a function in tachyzoite to bradyzoite conversion. In this study, we've employed various...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104488
更新日期:2020-12-01 00:00:00
abstract::Staphylococcus aureus small-colony variants (SCVs) and biofilms are linked to chronic infections. It is known that the presence of aminoglycoside antibiotics may contribute to the emergence of SCVs and it is thought that molecular mechanisms are involved in the ability of S. aureus to adopt this phenotype. No study ha...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2009.10.003
更新日期:2010-01-01 00:00:00
abstract::Medicinal plants are often the only therapeutic resource for many communities and ethnic groups. Cordia verbenacea DC., "Erva-baleeira," is one of the species of plants currently used to produce a phytotherapeutic product extracted from its leaves. The present study aimed to establish its chemical profile, antibacteri...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2016.03.009
更新日期:2016-06-01 00:00:00
abstract::Food plants Hungarian wax pepper (HWP) and Green Bell pepper (GBP), belonging to Capsicum annuum were utilized for biogenic fabrication of zero valent, nano-silver (AgNPs) through a photo-mediation procedure. In the bacterial strains evaluated, HWP/GBP AgNPs demonstrated effective bacteriostatic and bactericidal effec...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.08.053
更新日期:2018-11-01 00:00:00
abstract::This study aims to investigate whether IL-10 regulate decidual Treg cells apoptosis to reverse the abnormal pregnancy outcomes with Toxoplasma gondii (T. gondii) infection. Recombinant mouse IL-10 (rIL-10) treatment and IL-10 deficiency (IL-10(-/-)) abnormal pregnancy animal models with T. gondii infection were establ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2015.10.002
更新日期:2015-12-01 00:00:00
abstract::Salmonella are causes of livestock, poultry, and other animal diseases but they also have the potential to infect people. Currently, antibiotics are the first choice for treatment of Salmonella infections. Thus, the utility of phage has become the research focus for scientists for several reasons. There are efficient,...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.10.042
更新日期:2019-01-01 00:00:00
abstract::Chlamydia persistence is a viable but non-replicative stage, induced by several sub-lethal stressor agents, including beta-lactam antibiotics. So far, no data about the connection between doxycycline and chlamydial persistence has been described in literature. We investigated the ability of doxycycline to induce C. tr...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104347
更新日期:2020-10-01 00:00:00
abstract::Group A streptococci demonstrate a number of distinct ways to interact with the human fibrinolytic system to acquire unregulatable cell-surface enzymatic activity. Interactions between bacteria, fibrinogen, streptokinase and plasminogen resulted in acquisition of cell-associated enzymatic activity that can lyse fibrin...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/s0882-4010(95)90013-6
更新日期:1995-03-01 00:00:00
abstract::An inbred strain of the southern platyfish, Xiphophorus maculatus, was used as a host for Aeromonas hydrophila and Yersinia ruckeri infections. The infections were initiated by holding the platyfish in inoculation baths containing dilutions of virulent A. hydrophila or Y. ruckeri strains. Inoculating the platyfish in ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1996.0011
更新日期:1996-02-01 00:00:00
abstract::This study aimed to investigate antibiotic resistance and putative virulence factors among Cronobacter sakazakii isolated from powdered infant formula and other sources. The following 9 cultures (CR1-9) were collected from our culture collection: C. sakazakii and 3 Cronobacter species: C. sakazakii ATCC® 29544™, C. mu...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.103665
更新日期:2019-11-01 00:00:00
abstract::Group B Streptococcus (GBS) is an important pathogen and is associated with sepsis and meningitis in neonates and infants. An ex vivo model that facilitates observations of GBS interactions with multiple host cell types over time was used to study its pathogenicity. GBS infections were associated with profound reducti...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2013.04.008
更新日期:2013-08-01 00:00:00
abstract:UNLABELLED:The outcome of Helicobacter pylori infection has been related to specific virulence-associated bacterial genotypes. The vacuolating cytotoxin (vacA), cagA gene, oipA and babA2 gene are important virulence factor involving gastric diseases. The objective of this study was to assess the relationship between vi...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2015.01.008
更新日期:2015-03-01 00:00:00
abstract::As one of the dominant bacteria in the ocean, Vibrio play important roles in maintaining the aquatic ecosystem. In this study, we studied the phylogenetic relationships of 32 Vibrio based on the 16S rRNA genes sequences and utilized substrate immersing zymography method to detect the trend of protease production and c...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.03.018
更新日期:2019-06-01 00:00:00
abstract::Infections with the obligate intracellular bacterium Chlamydia trachomatis are characterized by avoidance of fusion between chlamydia-containing endosomes and lysosomes, bacterial persistence and development of post-infectious sequelae. In this report we show that C. trachomatis induces apoptosis in McCoy and HeLa cel...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.2001.0460
更新日期:2001-10-01 00:00:00
abstract::The present study investigated the possible correlation between carriage of the virulence genes esp and fsrb, production of hemolysin and gelatinase and biofilm formation in human vs. animal enterococcal isolates. A collection of 219 enterococcal isolates recovered from clinical and fecal surveillance samples of hospi...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2012.03.003
更新日期:2012-06-01 00:00:00
abstract::Murine gammaherpesvirus 72 (MHV-72) is a virus of wild rodents and serves as a convenient small animal model to understand the pathogenesis of Epstein-Barr virus (EBV) and human herpesvirus 8 (HHV8) infection. In laboratory mice MHV-72 causes an acute infection of lung epithelial cells and establishes the latency in B...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.2001.0441
更新日期:2001-08-01 00:00:00
abstract::In this experimental study, the Allium essential oil (AHEO) was extracted through the hydrodistillation method. AHEO components were identified through gas chromatography/mass spectrometry (GC/MS), and its antioxidant properties and total phenolic content were examined through the methods of 2,2-diphenyl-1-picrylhydra...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2017.11.055
更新日期:2018-01-01 00:00:00
abstract::Ehrlichia risticii, the causative agent of Potomac horse fever, is an obligate intracellular rickettsial organism. To understand the role of 55 and 51 kilodalton immunodominant antigens of E. risticii in strain variation, their genes from the 25-D and 90-12 strains were cloned, sequenced, and expressed in E. coli. Seq...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1998.0208
更新日期:1998-06-01 00:00:00