Abstract：
:Establishment of pulmonary Mycobacterium tuberculosis infection requires evasion of host innate defenses. In the lung alveoli, epithelial cells naturally resist uptake by the inhaled bacilli while macrophages patrol the epithelial surface and phagocytose foreign microbes. Alveolar microvascular endothelial cells, however, have not been examined as a potential point of direct interaction with the bacilli. It has been shown with other bacterial and viral lung pathogens that the lung endothelial cells are not only a point of interaction, but a source for intracellular replication and chronic infection by the pathogen. To investigate if endothelial cells are susceptible to M. tuberculosis infection, we examined attachment, internalization, and intracellular replication of M. tuberculosis bacilli in an immortalized human lung microvascular endothelial cell line (HULEC). By 6 h post-infection, 12% of infecting bacilli were associated with the HULEC monolayer cells. This was twice the association observed following a similar infection with cells from a human foreskin microvascular endothelial cell line (HMEC-1). As measured by survival after the addition of a high extracellular concentration of the aminoglycoside amikacin, approximately one-third of the associated bacilli were internalized and unavailable to the drug in both cell lines. Using electron microscopy, large numbers of bacilli were visible in the vacuoles of HULEC cells after 48 h post-infection; the presence of bacterial septa between adjacent mycobacteria suggests intracellular replication. These in vitro findings support the hypothesis that lung endothelial cells have the potential to participate in in vivo lung infections.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Mehta PK,Karls RK,White EH,Ades EW,Quinn FDdoi
10.1016/j.micpath.2006.05.002subject
Has Abstractpub_date
2006-08-01 00:00:00pages
119-24issue
2-3eissn
0882-4010issn
1096-1208pii
S0882-4010(06)00076-3journal_volume
41pub_type
杂志文章abstract：:High-affinity binding sites for P-fimbriated and for 075X-positive Escherichia coli were located in the human kidney. Frozen sections of normal human kidney were double-stained first with fluorochrome-labelled bacteria and then with fluorochrome-labelled nephron site-specific lectins or antibodies. The P-fimbriate rec...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(86)90019-7
更新日期：1986-04-01 00:00:00
abstract：:Naturally derived compounds represent a potential source of pharmacologically active drugs able to contrast different diseases, including gastric cancer, a multifactorial disease, in which the important role played by H. pylori infection has been demonstrated. Carexanes, stilbene derivatives, isolated from plants of t...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.05.007
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abstract：:β-1,3-glucan plays a role in non-albicans Candida species biofilm formation and survival of biofilm Candida to stresses. In this study, we evaluated the antibiofilm activity of β-1,3-glucanase, which can degrade poly-β(1 → 3)-glucose of non-albicans Candida species biofilms, on single and mixed species biofilm of non-...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.10.057
更新日期：2017-12-01 00:00:00
abstract：:Toxoplasma species are obligate intracellular protozoan which are responsible for induction of several forms of Toxoplasmosis in humans. The mechanisms responsible for the progression of the prolonged forms of Toxoplasmosis and associated pathologies are yet to be identified. However, previous studies proposed that im...
journal_title：Microbial pathogenesis
pub_type： 杂志文章,评审
doi：10.1016/j.micpath.2014.03.006
更新日期：2014-04-01 00:00:00
abstract：OBJECTIVE:Prompt genotyping of Mycobacterium tuberculosis (M. tuberculosis) is crucial for improving molecular epidemiological investigation of tuberculosis (TB). METHODS:We performed a retrospective study to evaluate the use of 24 loci MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number of tandem-re...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2016.12.025
更新日期：2017-02-01 00:00:00
abstract：:An mdaB mutant strain in a quinone reductase (MdaB) of Helicobacter hepaticus type strain ATCC51449 was constructed by insertional mutagenesis, and the MdaB protein was purified and compared to the Helicobacter pylori enzyme. While wild type H. hepaticus cells could tolerate 6% O(2) for growth, the mdaB strain was cle...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2006.08.005
更新日期：2008-02-01 00:00:00
abstract：:Protein E (PE) is a conserved entity observed in both nontypeable Haemophilus influenzae (NTHi) and encapsulated H. influenzae. This is a small surface lipoprotein, consisting of only 160 amino acids, involved in the adhesion of H. influenzae to various types of epithelial cells. A 384-bp-long fragment from NTHi PE wa...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.07.025
更新日期：2017-09-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(91)90045-c
更新日期：1991-09-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(87)90097-0
更新日期：1987-09-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2013.11.001
更新日期：2014-01-01 00:00:00
abstract：:Antimicrobial therapy against extensively drug-resistant (XDR) P. aeruginosa biofilms is less efficient compared to the treatment of equal bacterial counts of free-floating planktonic cells, which has become a serious threat in hospital environment. P. aeruginosa regulate their cooperative activities and physiological...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.08.017
更新日期：2017-10-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1997.0174
更新日期：1998-02-01 00:00:00
abstract：:Moritella viscosa is the aetiological agent of winter-ulcer disease in farmed salmonids in the North Atlantic. Previously, two major (typical and variant) genetic clades have been demonstrated within this bacterial species, one of which is almost solely related to disease in Atlantic salmon (Salmo salar). In the prese...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2014.09.014
更新日期：2014-12-01 00:00:00
abstract：:Meningococci can be classified by the presence of serotype and subtype antigens on the class 2/3 and class 1 outer membrane proteins, respectively. A typing system employing monoclonal antibodies and a WCE for detection of such antigens enabled us to sero- and/or subtype 255 out of 268 group B meningococci from 19 cou...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(88)90045-9
更新日期：1988-01-01 00:00:00
abstract：:In this study an attempt to address the effects of immunosuppression on pathogenesis of peste des petits ruminants (PPR) virus infection was undertaken. Cyclophosphamide and dexamethasone were used to immunosuppress the animals. The drug treated animals exhibited severe leukopaenia and lymphopaenia; one of the indicat...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2012.01.003
更新日期：2012-04-01 00:00:00
abstract：:Hap, an auto-transporter protein, is an antigenically conserved adhesion protein which is present on both typeable and nontypeable Haemophilus influenzae. This protein has central role in bacterial attachment to respiratory tract epithelial cells. A 1000bp C-terminal fragment of Hap passenger domain (HapS) from nontyp...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2016.06.033
更新日期：2016-09-01 00:00:00
abstract：:Interleukin-10 (IL-10) is an anti-inflammatory cytokine associated with the inhibition of HIV replication. IL-10 polymorphisms were found to be linked to drug-induced hepatotoxicity. Hence we examined the prevalence of IL-10 (-819C/T,-1082A/G) polymorphisms in a total of 165 HIV patients which included 34 patients wit...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103544
更新日期：2019-08-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2012.05.008
更新日期：2012-09-01 00:00:00
abstract：:Coccidiosis, caused by various species of genus Eimeria, is a major parasitic disease in chicken. The increasing resistance of these parasites to currently used anticoccidial drugs has stimulated the search for new methods of control. As part of this effort, a study was designed to see the anticoccidial effect of diff...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.01.053
更新日期：2017-03-01 00:00:00
abstract：:The first occurrences and dissemination of resistant microorganisms led to the inefficacy of many antibiotics, available in the market nowadays, therefore, the search for new substances with antimicrobial activity from natural sources has gained a great importance. The purpose of this work is to evaluate the antibacte...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.01.028
更新日期：2017-03-01 00:00:00
abstract：:In this study we compared the host response to Listeria monocytogenes in 129 REJ mice with listeria-resistant (C57Bl/6j) and susceptible (Balb/c) mouse strains. In all experiments mice were inoculated by the i.p. route. 129 REJ mice and Balb/c mice were sensitive to listeriosis whilst C57Bl/6j mice were relatively res...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1995.0032
更新日期：1995-05-01 00:00:00
abstract：:Salmonella enterica serovar Pullorum (S. Pullorum) is a worldwide poultry pathogen of considerable economic importance, particularly in those countries with a developing poultry industry. A variety of genes that affect S. Pullorum colonization in chickens had been identified. 2',3'-cyclic phosphodiesterase (cpdB) is t...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2015.01.002
更新日期：2015-03-01 00:00:00
abstract：:Mycoplasma pneumoniae (M. pneumoniae) is a small bacterium characterized by the absence of cell wall. It is a human pathogen causing upper and lower respiratory infections, both in adults and children. However, it is also considered to be implicated in the pathogenesis of several types of extra-respiratory diseases, i...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103863
更新日期：2020-02-01 00:00:00
abstract：:Medicinal plants are often the only therapeutic resource for many communities and ethnic groups. Cordia verbenacea DC., "Erva-baleeira," is one of the species of plants currently used to produce a phytotherapeutic product extracted from its leaves. The present study aimed to establish its chemical profile, antibacteri...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2016.03.009
更新日期：2016-06-01 00:00:00
abstract：:Viral myocarditis (VMC) is a type of inflammation affecting myocardial cells caused by viral infection and has been an important cause of dilated cardiomyopathy (DCM) worldwide. Type B3 coxsackievirus (CVB3), a non-enveloped positive-strand RNA virus of the Enterovirus genus, is one of most common agent of viral myoca...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2020.104169
更新日期：2020-07-01 00:00:00
abstract：BACKGROUND:Brucella is a Gram-negative and facultative intracellular organism that causes brucellosis, a common zoonotic disease. Over 500,000 people are annually affected by brucellosis. Brucella is highly infectious through inhalation route; for this reason it is used for biological warfare aims. This study aimed to ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.04.050
更新日期：2018-08-01 00:00:00
abstract：:In Salmonella enterica serovar Typhimurium, MgtC and PagC are positively regulated by the PhoP-PhoQ two-component system, which is activated under magnesium deprivation. Both MgtC and PagC are of unknown function but have been involved in intramacrophage survival. We have found that the amount of PagC is lowered in a ...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2008.06.001
更新日期：2008-09-01 00:00:00
abstract：:As a typical Gram-positive microorganism, S. aureus was recognized as common foodborne pathogenic bacteria in food industry. To study their individuality and pathogenicity mechanism, thirty-three Staphylococci strains were applied to the investigation with the identification of MRSA by PCR targeting on S. aureus speci...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2018.09.031
更新日期：2018-12-01 00:00:00
abstract：:It has been shown that N-linked high mannose type oligosaccharides competitively inhibits attachment to and infectivity of chlamydiae in HeLa cells. To further study whether mannose moieties are involved in the infectivity of chlamydiae, the susceptibility of mannose-receptor negative J774A and positive J774E mouse ma...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.2001.0479
更新日期：2002-01-01 00:00:00
abstract：INTRODUCTION:Tuberculosis (TB) is considered as a serious complication for organ transplant recipients; therefore, the detection and appropriate treatment of latent infection are recommended for preventing active TB infections in the future. The objective of this review is to conduct a systematic review and meta-analys...
journal_title：Microbial pathogenesis
pub_type： 杂志文章,meta分析
doi：10.1016/j.micpath.2018.09.040
更新日期：2018-12-01 00:00:00
