Myricetin inhibits the type III secretion system of Salmonella enterica serovar typhimurium by downregulating the Salmonella pathogenic island I gene regulatory pathway.


:Based on the in-depth study of type III secretion systems (T3SS) in pathogenic bacteria, approaches targeting T3SS have become new alternative strategies to combat drug-resistant bacterial infections. As an important food-borne pathogen, Salmonella enterica serovar Typhimurium (S. Typhimurium) injects effector proteins into host cells through the T3SS to disrupt cell signaling and host responses. In this study, myricetin was screened for its ability to block the translocation function of effector proteins (SipA/SipB) using cell biology and molecular biology methods. It exerted strong effects on inhibiting the expression of Salmonella pathogenicity island 1 (SPI-1)-associated effector proteins without affecting S. Typhimurium growth and thus prevented S. Typhimurium from invading HeLa cells and ultimately inhibited S. Typhimurium-mediated cell damage. In an animal experiment, myricetin comprehensively protected mice from death and pathological damage. A further analysis of the mechanism of action showed that myricetin interfered with the regulatory network of SPI-1-related genes, resulting in a significant decrease in the levels of key effector proteins, and thus inhibited T3SS-mediated virulence. In summary, this study provides a solution for clinical resistance to S. Typhimurium infection and potential candidate compounds. Myricetin, a potential T3SS inhibitor, possesses effective biological activity and exerts protective effects in vitro and in vivo. Myricetin will likely be developed as a novel type of antibiotic targeting S. Typhimurium infections in the future.


Microb Pathog


Microbial pathogenesis


Lv Q,Lv Y,Dou X,Wassy SL,Jia G,Wei L,Yu Q,Deng X,Zhang C,Wang J




Has Abstract


2021-01-05 00:00:00












  • Inflammatory response induced by Helicobacter pylori infection in lung.

    abstract::Helicobacter pylori is a microorganism that in the last years has been associated with extragastric disorders such as respiratory diseases, however, its impact on lung is partially understood. The aim of this work was to study infection impact of H. pylori on the inflammatory markers expression at the pulmonary level ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Arismendi Sosa AC,Salinas Ibáñez AG,Pérez Chaca MV,Penissi AB,Gómez NN,Vega AE

    更新日期:2020-02-26 00:00:00

  • Detection of antibodies against Aggregatibacter actinomycetemcomitans in serum and saliva through ELISA in periodontally healthy individuals and individuals with chronic periodontitis.

    abstract:BACKGROUND:Periodontitis is a persistent polymicrobial infection, which leads to chronic inflammation in the tooth supporting tissues. Aggregatibacter actinomycetemcomitans are normal commensals of oral cavity but are low in number in periodontally healthy subjects. They are one of the major pathogens aetiologically li...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Gadekar NB,Hosmani JV,Bhat KG,Kotrashetti VS,Nayak RS,Babji DV,Pattanshetty SM,Joshi VM,Bansode RA

    更新日期:2018-12-01 00:00:00

  • Impact of QseBC system in c-di-GMP-dependent quorum sensing regulatory network in a clinical isolate SSU of Aeromonas hydrophila.

    abstract::Our earlier studies showed that AhyRI- (AI-1) and LuxS-based (AI-2) quorum sensing (QS) systems were positive and negative regulators of virulence, respectively, in a diarrheal isolate SSU of Aeromonas hydrophila. Recently, we demonstrated that deletion of the QseBC two-component signal transduction system (AI-3 QS in...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Kozlova EV,Khajanchi BK,Popov VL,Wen J,Chopra AK

    更新日期:2012-09-01 00:00:00

  • Association between HLA-Ⅱgene polymorphism and Helicobacter pylori infection in Asian and European population: A meta-analysis.

    abstract:BACKGROUND:It is generally considered that HLA-Ⅱ genes contribute to the Helicobacter pylori (Hp) infection and disease development process. AIMS:To perform a meta-analysis to explore the relationship between HLA-Ⅱgene polymorphism and host susceptibility to Hp infection. METHODS:Relevant cohort studies, case-control...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,meta分析


    authors: Wang J,Zhang Q,Liu Y,Han J,Ma X,Luo Y,Liang Y,Zhang L,Hu Y

    更新日期:2015-05-01 00:00:00

  • Alteration of gene expression in human middle ear epithelial cells induced by influenza A virus and its implication for the pathogenesis of otitis media.

    abstract::Influenza A virus infection plays a significant role in the pathogenesis of Streptococcus pneumoniae-induced acute otitis media in children. An understanding of how influenza A virus modulates host cellular responses is critically important in efforts to explore the molecular mechanisms of this synergism. We used micr...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Tong HH,Long JP,Li D,DeMaria TF

    更新日期:2004-10-01 00:00:00

  • Identification of Synergistetes in endodontic infections.

    abstract::The bacterial phylum Synergistetes consists of Gram-negative anaerobes. Oral Synergistetes are divided in two main clusters, namely A and B. Increasing evidence demonstrates their involvement in etiology of oral infections, including apical periodontitis. This condition causes bone loss around the apex of the tooth, s...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Fernandes Cdo C,Rechenberg DK,Zehnder M,Belibasakis GN

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  • Structure, function and regulation of the thermostable direct hemolysin (TDH) in pandemic Vibrio parahaemolyticus.

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    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审


    authors: Cai Q,Zhang Y

    更新日期:2018-10-01 00:00:00

  • IL-10 regulate decidual Tregs apoptosis contributing to the abnormal pregnancy with Toxoplasma gondii infection.

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    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Lao K,Zhao M,Li Z,Liu X,Zhang H,Jiang Y,Wang Y,Hu X

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  • Characterization of outer membrane and secreted proteins of Leptospira interrogans serovar pomona.

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    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Zuerner RL,Knudtson W,Bolin CA,Trueba G

    更新日期:1991-04-01 00:00:00

  • Pros and cons of direct genotyping on tuberculosis clinical samples.

    abstract:OBJECTIVE:Prompt genotyping of Mycobacterium tuberculosis (M. tuberculosis) is crucial for improving molecular epidemiological investigation of tuberculosis (TB). METHODS:We performed a retrospective study to evaluate the use of 24 loci MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number of tandem-re...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Sadegh H,Kargarpour Kamakoli M,Farmanfarmaei G,Masoumi M,Abdolrahimi F,Fateh A,Ebrahimzadeh N,Rahimi Jamnani F,Vaziri F,Siadat SD

    更新日期:2017-02-01 00:00:00

  • Epigallocatechin gallate can attenuate human alveolar epithelial cell injury induced by alpha-haemolysin.

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    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Zhou Y,Chen C,Pan J,Deng X,Wang J

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  • In vitro anti-Candida activity of selective serotonin reuptake inhibitors against fluconazole-resistant strains and their activity against biofilm-forming isolates.

    abstract::Recent research has shown broad antifungal activity of the classic antidepressants selective serotonin reuptake inhibitors (SSRIs). This fact, combined with the increased cross-resistance frequency of the genre Candida regarding the main treatment today, fluconazole, requires the development of novel therapeutic strat...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


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  • Opa (protein II) influences gonococcal organization in colonies, surface appearance, size and attachment to human fallopian tube tissues.

    abstract::Opa-expressing variants of Neisseria gonorrhoeae strain F62-SF and an Opa- variant, all non-piliated, were examined for differences in the interaction of the bacteria within colonies and in attachment to and damage of human fallopian tube mucosa. Expression of certain Opas was associated with the formation of transpar...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Dekker NP,Lammel CJ,Mandrell RE,Brooks GF

    更新日期:1990-07-01 00:00:00

  • Protective immunity in mouse salmonellosis: comparison of smooth and rough live and killed vaccines.

    abstract::Protective immunity against Salmonella infection was studied in a mouse model. To study specificity of protection we used smooth (O-4,5,12) and rough vaccines; live and killed vaccines of both types were compared. The protection was assessed by enumerating the number of bacteria in the livers, and by following surviva...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Muotiala A,Hovi M,Mäkelä PH

    更新日期:1989-01-01 00:00:00

  • Antioxidant and immunostimulatory effect of potential probiotic Lactobacillus paraplantarum SC61 isolated from Korean traditional fermented food, jangajji.

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    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Son SH,Yang SJ,Jeon HL,Yu HS,Lee NK,Park YS,Paik HD

    更新日期:2018-12-01 00:00:00

  • Preparation and evaluation of antibacterial potential of Pithecellobium dulce root extract against Gram positive and Gram negative bacteria.

    abstract::In the present study hexane, benzene, ethyl acetate and ethanol extracts of Pithecellobium dulce root were prepared using soxhlet extractor. The extracts were evaluated for antibacterial activity against one Gram positive (Staphylococcus aureus) and three Gram negative (Acetobacter aceti, Acetobacter aceti, Klebsiella...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Bhat MA,Malik RA,Prakash P,Lone AM

    更新日期:2018-03-01 00:00:00

  • In vitro studies of epithelium-associated crystallization caused by uropathogens during urinary calculi development.

    abstract::Infectious urinary stones account for about 10% of all urinary stones. In 50% of cases urolithiasis is a recurrent illness, which can lead to the loss of a kidney if not properly treated. One of the reasons for recurrence of the disease may be the ability of bacteria to invade urothelial cells, persist in the host cel...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Torzewska A,Budzyńska A,Białczak-Kokot M,Różalski A

    更新日期:2014-06-01 00:00:00

  • Deletions of chromosomal regions coding for fimbriae and hemolysins occur in vitro and in vivo in various extraintestinal Escherichia coli isolates.

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    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Hacker J,Bender L,Ott M,Wingender J,Lund B,Marre R,Goebel W

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  • South Indian medicinal plants can combat deadly viruses along with COVID-19? - A review.

    abstract::SARS-CoV-2 is a causative agent of Coronavirus disease-19 (COVID-19), which is considered as a fatal disease for public health apprehension worldwide. This pathogenic virus can present everywhere. As it is a virus it can extend easily and cause severe illness to humans. Hence, an efficient international attentiveness ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Divya M,Vijayakumar S,Chen J,Vaseeharan B,Durán-Lara EF

    更新日期:2020-11-01 00:00:00

  • Pathophysiology of peste des petits ruminants in sheep (Dorper & Kajli) and goats (Boer & Beetal).

    abstract::Peste des petits ruminants (PPR), an economically important viral transboundary disease of small ruminants is not only prevalent in Pakistan but also in other countries where people rely on agriculture and animal products. The present study was aimed at describing the pathology and antigen localization in natural PPR ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Khan A,Saleemi MK,Ali F,Abubakar M,Hussain R,Abbas RZ,Khan IA

    更新日期:2018-04-01 00:00:00

  • A systematic review of Toxoplasma gondii antigens to find the best vaccine candidates for immunization.

    abstract::At present, there is not any available accepted vaccine for prevention of Toxoplasma gondii (T. gondii) in human and animals. We conducted literature search through English (Google Scholar, PubMed, Science Direct, Scopus, EBSCO, ISI Web of Science) scientific paper databases to find the best vaccine candidates against...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Rezaei F,Sarvi S,Sharif M,Hejazi SH,Pagheh AS,Aghayan SA,Daryani A

    更新日期:2019-01-01 00:00:00

  • First report of novel genetic array aacA4-blaIMP-25-oxa30-catB3 and identification of novel metallo-β-lactamase gene blaIMP25: A Retrospective Study of antibiotic resistance surveillance on Psuedomonas aeruginosa in Guangzhou of South China, 2003-2007.

    abstract::Carbapenem, imipenem and meropenem have been broadly prescribed contributing to the global occurrence and prevalence of carbapenem resistance in Psuedomonas aeruginosa, and the associated resistance genotypes remains clinically significant. A retrospective surveillance had been conducted on 499 P. aeruginosa isolates ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Yu G,Wen W,Peters BM,Liu J,Ye C,Che Y,Liu J,Cao K,Xu Z,Shirtliff ME

    更新日期:2016-06-01 00:00:00

  • Mycoplasma pneumoniae-related hepatitis in children.

    abstract::Mycoplasma pneumoniae (M. pneumoniae) is a small bacterium characterized by the absence of cell wall. It is a human pathogen causing upper and lower respiratory infections, both in adults and children. However, it is also considered to be implicated in the pathogenesis of several types of extra-respiratory diseases, i...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Poddighe D

    更新日期:2020-02-01 00:00:00

  • Local transient induction of inflammatory cytokines after intranasal administration of recombinant Bordetella pertussis.

    abstract::Inflammatory cytokines have been described to play a critical role in the orientation and amplification of the IgA immune response. In this study, we show that the intranasal administration of a Bordetella pertussis strain expressing the protective antigen glutathione-S-transferase of Schistosoma mansoni (Sm28GST) ind...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


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    更新日期:1997-05-01 00:00:00

  • Dendrimer-enabled transformation of Chlamydia trachomatis.

    abstract::Lack of a system for genetic manipulation of Chlamydia trachomatis has been a key challenge to advancing understanding the molecular genetic basis of virulence for this bacterial pathogen. We developed a non-viral, dendrimer-enabled system for transformation of this organism and used it to characterize the effects of ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Kannan RM,Gérard HC,Mishra MK,Mao G,Wang S,Hali M,Whittum-Hudson JA,Hudson AP

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  • Acinetobacter nosocomialis secretes outer membrane vesicles that induce epithelial cell death and host inflammatory responses.

    abstract::Acinetobacter nosocomialis is an important nosocomial pathogen that causes a variety of opportunistic infections; however, pathogenesis of this microorganism has not yet been characterized. The aim of this study was to investigate the secretion of outer membrane vesicles (OMVs) from A. nosocomialis and to determine th...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Nho JS,Jun SH,Oh MH,Park TI,Choi CW,Kim SI,Choi CH,Lee JC

    更新日期:2015-04-01 00:00:00

  • Transcriptional regulation of Yersinia pestis biofilm formation.

    abstract::Yersinia pestis, the causative agent of plague, is transmitted primarily by infected fleas in nature. Y. pestis can produce biofilms that block flea's proventriculus and promote flea-borne transmission. Transcriptional regulation of Y. pestis biofilm formation plays an important role in the response to complex changes...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审


    authors: Liu L,Zheng S

    更新日期:2019-06-01 00:00:00

  • A hemA mutation renders Salmonella typhimurium avirulent in mice, yet capable of eliciting protection against intravenous infection with S. typhimurium.

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    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Benjamin WH Jr,Hall P,Briles DE

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  • Functional analysis of pcpme6 from oomycete plant pathogen Phytophthora capsici.

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    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Feng B,Li P,Wang H,Zhang X

    更新日期:2010-07-01 00:00:00

  • The cold shock family gene cspD3 is involved in the pathogenicity of Ralstonia solanacearum CQPS-1 to tobacco.

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    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Liu Y,Tan X,Cheng H,Gong J,Zhang Y,Wang D,Ding W

    更新日期:2020-02-20 00:00:00