Abstract:
:Our earlier studies showed that AhyRI- (AI-1) and LuxS-based (AI-2) quorum sensing (QS) systems were positive and negative regulators of virulence, respectively, in a diarrheal isolate SSU of Aeromonas hydrophila. Recently, we demonstrated that deletion of the QseBC two-component signal transduction system (AI-3 QS in enterohemorrhagic Escherichia coli) also led to an attenuation of A. hydrophila in a septicemic mouse model of infection, and that interplay exists between AI-1, AI-2, and the second-messenger cyclic-di-guanosine monophosphate (c-di-GMP) in modulating bacterial virulence. To further explore a network connection between all of the three QS systems in A. hydrophila SSU and their cross talk with c-di-GMP, we overproduced a protein with a GGDEF domain, which increases c-di-GMP levels in bacteria, and studied phenotypes and transcriptional profiling of genes involved in biofilm formation and motility of the wild-type (WT) A. hydrophila and its ΔqseB mutant. Over-expression of the GGDEF domain-encoding gene (aha0701h) resulted in a significantly reduced motility of the WT A. hydrophila similar to that of the ΔqseB mutant. While enhanced protease production was noted in WT A. hydrophila that had increased c-di-GMP, no enzymatic activity was detected in the ΔqseB mutant overexpressing the aha0701h gene. Likewise, denser biofilm formation was noted for WT bacteria when c-di-GMP was overproduced compared to its respective control; however, overproduction of c-di-GMP in the ΔqseB mutant led to reduced biofilm formation, a finding similar to that noted for the parental A. hydrophila strain. These effects on bacterial motility and biofilm formation in the ΔqseB mutant or the mutant with increased c-di-GMP were correlated with altered levels of fleN and vpsT genes. While we noted transcript levels of qseB and qseC genes to be increased in the ahyRI mutant, down-regulation of the ahyR and ahyI genes was observed in the ΔqseB mutant, which correlated with decreased protease activity. Finally, an enhanced virulence of WT A. hydrophila with increased c-di-GMP was noted in a mouse model when compared to findings in the parental strain with vector alone. Overall, we conclude that cross talk between AI-1 and QseBC systems exists in A. hydrophila SSU, and c-di-GMP modulation on QseBC system is dependent on the expression of the AI-1 system.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Kozlova EV,Khajanchi BK,Popov VL,Wen J,Chopra AKdoi
10.1016/j.micpath.2012.05.008subject
Has Abstractpub_date
2012-09-01 00:00:00pages
115-24issue
3-4eissn
0882-4010issn
1096-1208pii
S0882-4010(12)00111-8journal_volume
53pub_type
杂志文章abstract:BACKGROUND:Tuberculosis (TB) remains a major public health problem. SH3RF1 and SH3RF2 are candidate genes with multiple single-nucleotide polymorphisms (SNPs) that have the potential to participate in Mycobacterium infection via activation of the JNK signaling pathway. In this case-control study, we aimed to investigat...
journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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abstract::Capnocytophaga canimorsus is a part of healthy oral flora of dogs and cats. However, when it is transmitted to human subjects via animal bites or scratches, it can cause severe complications like endocarditis or even lethal septic shock, especially in immunocompromised persons. In this study, we performed the first wh...
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abstract::During the past decade, cationic antimicrobial peptides (CAPs) have gained particular interest among researchers, since they often display broad-spectrum antimicrobial activity and low possibility of resistance emergence. This study aimed to investigate in vitro effectiveness of Mastoparan-1 (MP-1), a tetradecapeptide...
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journal_title:Microbial pathogenesis
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更新日期:1992-06-01 00:00:00
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更新日期:2018-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.micpath.2016.02.021
更新日期:2016-06-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2014.05.001
更新日期:2014-08-01 00:00:00
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doi:10.1016/j.micpath.2014.04.007
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pub_type: 杂志文章,评审
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更新日期:2017-02-01 00:00:00
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更新日期:2020-11-15 00:00:00
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journal_title:Microbial pathogenesis
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更新日期:2016-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0882-4010(89)90110-1
更新日期:1989-07-01 00:00:00
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更新日期:2020-01-01 00:00:00
abstract::Klebsiella pneumoniae is one of the most important agents of community-acquired urinary tract infection (CA-UTI). In addition to extended-spectrum β-lactamases (ESBLs), a number of virulence factors have been shown to play an important role in the pathogenesis of K. pneumoniae, including capsule, siderophores, and adh...
journal_title:Microbial pathogenesis
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doi:10.1016/j.micpath.2016.10.002
更新日期:2016-11-01 00:00:00
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journal_title:Microbial pathogenesis
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abstract:BACKGROUND:Since the pre-transplant status affects the renal transplantation success and ultimately the survival rate, identifying the probable risk factors that increase the chance of BK virus replication in end-stage renal disease patients can be included in proposing proper surveillance guidelines during pre and pos...
journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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