Abstract:
OBJECTIVE:Prompt genotyping of Mycobacterium tuberculosis (M. tuberculosis) is crucial for improving molecular epidemiological investigation of tuberculosis (TB). METHODS:We performed a retrospective study to evaluate the use of 24 loci MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number of tandem-repeat) directly on 135 clinical samples from 84 TB patients. RESULTS:There was a direct correlation between genotyping on clinical samples by MIRU-VNTR and bacterial load (P = 0.001). VNTR loci were amplified successfully for 41.5% of the clinical samples (19-24 loci), 32.6% (13-18 loci), 23.7% (7-12 loci) and 2.2% (1-6 loci). Loci of 2401, 577, 2996 and 154 had the highest power to show the mixed strains infection in clinical samples. CONCLUSIONS:Direct MIRU-VNTR is partially successful in complete genotyping of M. tuberculosis strains. On the other hand, detection of polyclonal infection is undoubtedly reliable based on the direct MIRU-VNTR.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Sadegh H,Kargarpour Kamakoli M,Farmanfarmaei G,Masoumi M,Abdolrahimi F,Fateh A,Ebrahimzadeh N,Rahimi Jamnani F,Vaziri F,Siadat SDdoi
10.1016/j.micpath.2016.12.025subject
Has Abstractpub_date
2017-02-01 00:00:00pages
135-138eissn
0882-4010issn
1096-1208pii
S0882-4010(16)30622-2journal_volume
103pub_type
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journal_title:Microbial pathogenesis
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