Effect of porphyrins and host iron transport proteins on outer membrane protein expression in Porphyromonas (Bacteroides) gingivalis: identification of a novel 26 kDa hemin-repressible surface protein.

Abstract:

:Porphyromonas gingivalis is capable of in vitro growth when iron sources are either complexed to hemin or host iron transport proteins, or exist in an inorganic form. This study examined the effect of these iron sources on outer membrane protein (OMP) expression in P. gingivalis W50. Hemin (iron) starved P. gingivalis was transferred into growth medium containing hemin, hemoglobin, hemin-saturated human serum albumin, hemin-free human serum albumin, transferrin, lactoferrin, or inorganic iron. Surface proteins were identified by 125I-labeling and resolved by SDS-PAGE and autoradiography. When grown under hemin starved conditions, P. gingivalis W50 and related strains expressed a major 26 kDa OMP, as revealed by 125I-autoradiography. Autoradiographic analysis demonstrated the absence of this 26 kDa OMP from the P. gingivalis surface in hemin-containing environments. Growth of P. gingivalis W50 in the presence of host iron transport proteins (hemin-free) or inorganic iron resulted in surface expression of a 26 kDa OMP. The presence of protoporphyrin IX or substitution of hemin-associated iron with zinc, resulted in continued surface expression of the 26 kDa OMP, indicating that repressibility of this OMP required the coordination of iron to the protoporphyrin IX molecule (i.e. hemin). A survey of 125I-labeled OMPs from several hemin starved P. gingivalis and related strains, demonstrated that a hemin-repressible 26 kDa OMP occurred only in P. gingivalis. We report here a newly described 26 kDa hemin-regulated surface protein occurring in several strains of P. gingivalis which is expressed on the cell surface in hemin starved conditions and is lost from the cell surface in response to an environment containing iron coordinated specifically to protoporphyrin IX (i.e. hemin).

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Bramanti TE,Holt SC

doi

10.1016/0882-4010(92)90032-j

subject

Has Abstract

pub_date

1992-07-01 00:00:00

pages

61-73

issue

1

eissn

0882-4010

issn

1096-1208

pii

0882-4010(92)90032-J

journal_volume

13

pub_type

杂志文章
  • Recombinant truncated E protein as a new vaccine candidate against nontypeable H. influenzae: Its expression and immunogenic evaluation.

    abstract::Protein E (PE) is a conserved entity observed in both nontypeable Haemophilus influenzae (NTHi) and encapsulated H. influenzae. This is a small surface lipoprotein, consisting of only 160 amino acids, involved in the adhesion of H. influenzae to various types of epithelial cells. A 384-bp-long fragment from NTHi PE wa...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2017.07.025

    authors: Behrouzi A,Bouzari S,Vaziri F,Fateh A,Afrough P,Vijeh Motlagh AD,Siadat SD

    更新日期:2017-09-01 00:00:00

  • Species and strain specificity of Lactobacillus probiotics effect on weight regulation.

    abstract::Certain strains of Lactobacillus appear to have a reproducible effect on weight as a weight-gain effect in lean humans and animals or a weight-loss effect in overweight/obese humans and animals. These results are completely sufficient to capture the attention of the scientific community to assess the effect on the wei...

    journal_title:Microbial pathogenesis

    pub_type: 评论,信件

    doi:10.1016/j.micpath.2012.09.013

    authors: Million M,Raoult D

    更新日期:2013-02-01 00:00:00

  • Evidence for group A-related M protein genes in human but not animal-associated group G streptococcal pathogens.

    abstract::Group G streptococci have on their surface antiphagocytic M protein-like antigens. To determine if these organisms have genes similar to the M protein genes of Streptococcus pyogenes (group A), DNA from independent group G isolates of human and animal origin were tested for homology to probes representing sequences en...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(87)90004-0

    authors: Simpson WJ,Robbins JC,Cleary PP

    更新日期:1987-11-01 00:00:00

  • The genus Aeromonas: A general approach.

    abstract::The genus Aeromonas comprises more than thirty Gram-negative bacterial species which mostly act as opportunistic microorganisms. These bacteria are distributed naturally in diverse aquatic ecosystems, where they are easily isolated from animals such as fish and crustaceans. A capacity for adaptation also makes Aeromon...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审

    doi:10.1016/j.micpath.2019.02.036

    authors: Gonçalves Pessoa RB,de Oliveira WF,Marques DSC,Dos Santos Correia MT,de Carvalho EVMM,Coelho LCBB

    更新日期:2019-05-01 00:00:00

  • Visfatin regulates the production of lipopolysaccharide-induced inflammatory cytokines through p38 signaling in murine macrophages.

    abstract::Visfatin plays an important role in regulation of inflammatory cytokines. However, the role of visfatin under bacterial stress condition is not fully explored yet. Therefore, the present study was conducted for the better understanding of the regulation mechanism of visfatin on the production of inflammatory cytokines...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.02.002

    authors: Wu XT,Yang Z,Ansari AR,Xiao K,Pang XX,Luo Y,Song H

    更新日期:2018-04-01 00:00:00

  • Evaluation of antibacterial and antioxidant potential of the zinc oxide nanoparticles synthesized by aqueous and polyol method.

    abstract::In this paper, we have reported the synthesis, characterization, and evaluation of antimicrobial and antioxidant potential of monodispersed Zinc Oxide (ZnO) nanoparticles synthesized by the room temperature precipitation (aqueous phase) and polyol method (organic phase). ZnO nanoparticle synthesized by both the method...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.03.048

    authors: Soren S,Kumar S,Mishra S,Jena PK,Verma SK,Parhi P

    更新日期:2018-06-01 00:00:00

  • Alteration of gene expression in human middle ear epithelial cells induced by influenza A virus and its implication for the pathogenesis of otitis media.

    abstract::Influenza A virus infection plays a significant role in the pathogenesis of Streptococcus pneumoniae-induced acute otitis media in children. An understanding of how influenza A virus modulates host cellular responses is critically important in efforts to explore the molecular mechanisms of this synergism. We used micr...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2004.06.012

    authors: Tong HH,Long JP,Li D,DeMaria TF

    更新日期:2004-10-01 00:00:00

  • Structural deformation in pathogenic bacteria cells caused by marine fungal metabolites: An in vitro investigation.

    abstract::Over the past 50 years, fungal natural products have revolutionized medicine, yielding drugs which have enormous therapeutic potential. The aim of this study was to investigate the probable effect of marine fungal natural products on various skin pathogens. Initially, seventy natural extracts obtained from 35 differen...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104248

    authors: Agrawal S,Barrow CJ,Deshmukh SK

    更新日期:2020-09-01 00:00:00

  • Quantitation of H. pylori cytotoxin mRNA by real-time RT-PCR shows a wide expression range that does not correlate with promoter sequences.

    abstract::Up to 28-fold differences in vacA expression in Helicobacter pylori strains grown in vitro were demonstrated by real time quantitative RT-PCR. These large differences in expression were unrelated to putative -35 and -10 motifs or to other untranslated sequences upstream of the ATG start site. The lack of correlation b...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2004.06.003

    authors: Ayala G,Chihu L,Perales G,Fierros-Zárate G,Hansen LM,Solnick JV,Sánchez J

    更新日期:2004-09-01 00:00:00

  • Location of adhesion sites for P-fimbriated and for 075X-positive Escherichia coli in the human kidney.

    abstract::High-affinity binding sites for P-fimbriated and for 075X-positive Escherichia coli were located in the human kidney. Frozen sections of normal human kidney were double-stained first with fluorochrome-labelled bacteria and then with fluorochrome-labelled nephron site-specific lectins or antibodies. The P-fimbriate rec...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(86)90019-7

    authors: Nowicki B,Holthöfer H,Saraneva T,Rhen M,Väisänen-Rhen V,Korhonen TK

    更新日期:1986-04-01 00:00:00

  • Cloning and molecular analysis of genes encoding two immunodominant antigens of Ehrlichia risticii.

    abstract::Ehrlichia risticii, the causative agent of Potomac horse fever, is an obligate intracellular rickettsial organism. To understand the role of 55 and 51 kilodalton immunodominant antigens of E. risticii in strain variation, their genes from the 25-D and 90-12 strains were cloned, sequenced, and expressed in E. coli. Seq...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1998.0208

    authors: Vemulapalli R,Biswas B,Dutta SK

    更新日期:1998-06-01 00:00:00

  • Nutritional interference for phenotypic biofilm quantification in Aeromonas spp. isolates containing the fla gene.

    abstract::The Aeromonas genus has several virulence factors associated with the development of diseases in aquatic organisms, leading to losses in aquaculture. One of these factors is the flagella's formation which allows the biofilm's formation that provides the microorganisms a greater pathogenicity, greater protection to cer...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.11.044

    authors: Freire NB,Magalhães TC,Nunes Soares RA,da Costa MM,Gouveia GV

    更新日期:2019-02-01 00:00:00

  • Mice sublethally infected with Francisella novicida U112 develop only marginal protective immunity against systemic or aerosol challenge with virulent type A or B strains of F. tularensis.

    abstract::The current study determined the ability of Francisella novicida to act as a live vaccine against the much more virulent, but closely related pathogen, Francisella tularensis. Live attenuated strains of the latter are effective vaccines against human tularemia. However, the molecular cause of their attenuation remains...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2004.04.005

    authors: Shen H,Chen W,Conlan JW

    更新日期:2004-08-01 00:00:00

  • Host-directed therapies to combat tuberculosis and associated non-communicable diseases.

    abstract::Mycobacterium tuberculosis (Mtb) has coevolved with a human host to evade and exploit the immune system in multiple ways. Mtb is an enormously successful human pathogen that can remain undetected in hosts for decades without causing clinical disease. While tuberculosis (TB) represents a perfect prototype of host-patho...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审

    doi:10.1016/j.micpath.2019.03.003

    authors: Baindara P

    更新日期:2019-05-01 00:00:00

  • Molecular and biochemical mechanisms of Pasteurella haemolytica leukotoxin-induced cell death.

    abstract::Pasteurella haemolytica leukotoxin (LKT) is a member of the RTX family of pore-forming toxins that kill bovine immune cells. Several studies have suggested that RTX toxins kill target cells by the induction of apoptosis. In the present study, BL3 bovine leukaemia cells were exposed to LKT and assessed by molecular and...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1998.0236

    authors: Wang JF,Kieba IR,Korostoff J,Guo TL,Yamaguchi N,Rozmiarek H,Billings PC,Shenker BJ,Lally ET

    更新日期:1998-12-01 00:00:00

  • Identification of 60 secreted proteins for Mycoplasma bovis with secretome assay.

    abstract::Mycoplasma bovis is a risky pathogen mainly responsible for pneumonia and mastitis in cattle. Up to date, its pathogenesis is not clear. Since secreted proteins have a tricky role in M. bovis pathogenesis, this study was designed to systematically reveal M. bovis secretome and potential role in virulence of the pathog...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104135

    authors: Zubair M,Muhamed SA,Khan FA,Zhao G,Menghwar H,Faisal M,Zhang H,Zhu X,Rasheed MA,Chen Y,Marawan MA,Chen H,Guo A

    更新日期:2020-06-01 00:00:00

  • Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining.

    abstract::Limited deep studies are available in the field of early stages of pathogenesis of Newcastle disease virus (NDV) infection and tissue tropism of NDV. In this study, 24 specific pathogen free (SPF) chickens of white leghorn breed were infected with Newcastle disease (ND) by intranasal administration of 10⁵ 50% EID50/0....

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2019.02.017

    authors: Hussein EA,Hair-Bejo M,Omar AR,Arshad SS,Hani H,Balakrishnan KN,Yakubu Y,Saeed MI,Aini I

    更新日期:2019-04-01 00:00:00

  • Complete genome sequence of Bacillus licheniformis BL-010.

    abstract::The biodegradation of Aflatoxin B1 (AFB1) is an industry of increasing importance. Bacillus licheniformis BL-010 was isolated from the aflatoxin contaminated corn feed storage, and was shown to degrade AFB1 efficiently. Here we present the complete genome sequence of BL-010, the genome comprises 4,287,714 bp in a circ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.03.037

    authors: He S,Feng K,Ding T,Huang K,Yan H,Liu X,Zhang Z

    更新日期:2018-05-01 00:00:00

  • Genetic diversity and virulence characteristics of Staphylococcus aureus isolates from cases of bovine mastitis.

    abstract::Staphylococcus aureus is one of the major bacterial mastitis pathogens with significant effects on animal and human health. Some studies showed that S. aureus strains that infect different host species are genetically distinct, although most strains can infect a wide range of host species. However, there are no clearl...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104171

    authors: Vaughn JM,Abdi RD,Gillespie BE,Kerro Dego O

    更新日期:2020-07-01 00:00:00

  • The role of Listeria monocytogenes cell wall surface anchor protein LapB in virulence, adherence, and intracellular replication.

    abstract::Lmof2365_2117 is a Listeria monocytogenes putative cell wall surface anchor protein with a conserved domain found in collagen binding proteins. We constructed a deletion mutation in lmof2365_2117 in serotype 4b strain F2365, evaluated its virulence, and determined its ability to adhere and invade colonic epithelial ce...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2015.12.012

    authors: Reddy S,Akgul A,Karsi A,Abdelhamed H,Wills RW,Lawrence ML

    更新日期:2016-03-01 00:00:00

  • Antitumor effect of exopolysaccharide produced by Bacillus mycoides.

    abstract::Recently, microbial exopolysaccharides (EPSs) have offered very large field for medical applications owing to their bioactive characteristics. This study aimed to obtain antitumor EPS and to optimize its production using different optimization approaches. Eighty EPSs-producing bacteria were obtained from mud samples. ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2019.103947

    authors: Farag MMS,Moghannem SAM,Shehabeldine AM,Azab MS

    更新日期:2020-03-01 00:00:00

  • Quantification of Staphylococcus aureus cell surface adhesins using flow cytometry.

    abstract::The initiation of many infectious diseases involves specific adhesion of bacteria to host tissue proteins and carbohydrates. Staphylococcus aureus is known to bind specifically to several proteins in the extracellular matrix (ECM). We report the quantification of the collagen and fibronectin adhesin densities on the s...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.2000.0399

    authors: Mohamed N,Visai L,Speziale P,Ross JM

    更新日期:2000-12-01 00:00:00

  • Broad-spectrum quorum sensing and biofilm inhibition by green tea against gram-negative pathogenic bacteria: Deciphering the role of phytocompounds through molecular modelling.

    abstract::The emerging prevalence of multidrug-resistance in Gram-negative pathogens, due to conventional antimicrobial therapeutics, has led the researchers to emphasize on development of alternative novel strategies to suppress the bacterial virulence and pathogenicity through inhibition of quorum sensing (QS) and biofilms. Q...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.11.030

    authors: Qais FA,Khan MS,Ahmad I

    更新日期:2019-01-01 00:00:00

  • Isolation and characterization of the non-fimbrial adhesin NFA-4 from uropathogenic Escherichia coli O7:K98:H6.

    abstract::The non-fimbrial adhesin NFA-4 from uropathogenic Escherichia coli O7:K98:H6 mediates the agglutination of human red cells (RBC), notably of blood group MM. The adhesin can be separated from the bacteria by heat extraction and was purified to homogeneity by ammonium sulphate precipitation and anion exchange chromatogr...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(89)90077-6

    authors: Hoschützky H,Nimmich W,Lottspeich F,Jann K

    更新日期:1989-05-01 00:00:00

  • Reactivity of Haemophilus influenzae type b anti-pili antibodies.

    abstract::The reactivity of anti-pilus antibodies to native and denatured Haemophilus influenzae b (Hib) pili was studied using rabbit serum prepared against piliated H. influenzae b strain M43 (p+) and adsorbed with its non-piliated variant, strain M42 (p-). The specificity of the adsorbed serum for Hib pili was documented by ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(89)90049-1

    authors: Gilsdorf JR,Forney LJ,LiPuma JJ

    更新日期:1989-10-01 00:00:00

  • Induction of monocyte chemoattractant protein-1 (MCP-1) production by Pseudomonas nitrite reductase in human pulmonary type II epithelial-like cells.

    abstract::Monocyte chemoattractant protein-1 (MCP-1), a chemoattractant for monocytes, is presumed to play a pivotal role in the recruitment and accumulation of monocytes in various diseases including pulmonary infections. We examined here whether or not Pseudomonas nitrite reductase (PNR), a recently identified IL-8 inducer in...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1999.0320

    authors: Sar B,Oishi K,Wada A,Hirayama T,Matsushima K,Nagatake T

    更新日期:2000-01-01 00:00:00

  • Study of serum bactericidal and splenic activity of Total-OMP- CagA combination from Brucella abortus and Helicobacter pylori in BALB/c mouse model.

    abstract:BACKGROUND:Brucella is a Gram-negative and facultative intracellular organism that causes brucellosis, a common zoonotic disease. Over 500,000 people are annually affected by brucellosis. Brucella is highly infectious through inhalation route; for this reason it is used for biological warfare aims. This study aimed to ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.04.050

    authors: Abadi AH,Mahdavi M,Khaledi A,Esmaeili SA,Esmaeili D,Sahebkar A

    更新日期:2018-08-01 00:00:00

  • Characterization of potential lytic bacteriophage against Vibrio alginolyticus and its therapeutic implications on biofilm dispersal.

    abstract::Vibrio alginolyticus is a leading cause of vibriosis, presenting opportunistic infections to humans associated with raw seafood contamination. At present, phage therapy that acts as an alternative sanitizing agent is explored for targeting V. alginolyticus. The study outcome revealed that the phage VP01 with its extre...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2016.10.017

    authors: Sasikala D,Srinivasan P

    更新日期:2016-12-01 00:00:00

  • Myricetin inhibits the type III secretion system of Salmonella enterica serovar typhimurium by downregulating the Salmonella pathogenic island I gene regulatory pathway.

    abstract::Based on the in-depth study of type III secretion systems (T3SS) in pathogenic bacteria, approaches targeting T3SS have become new alternative strategies to combat drug-resistant bacterial infections. As an important food-borne pathogen, Salmonella enterica serovar Typhimurium (S. Typhimurium) injects effector protein...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104695

    authors: Lv Q,Lv Y,Dou X,Wassy SL,Jia G,Wei L,Yu Q,Deng X,Zhang C,Wang J

    更新日期:2021-01-05 00:00:00

  • Production and characterization of monoclonal antibodies against GII.6 norovirus virus-like particles.

    abstract::In this study we generated and characterized a series of monoclonal antibodies (mAbs) against GII.6 norovirus (NoV) virus like particles (VLPs). Mice were immunized with purified GII.6 NoV VLPs and peptide-bovine serum albumin (BSA) conjugates with the peptide sequence (31 aa) derived from the trypsin cleavage region....

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104100

    authors: Qiu S,Zheng L,Qin C,Yin X,Ma J,Liu J,Yang Z,Li C,Wang Y,Wang M,Qi Y,Huo Y

    更新日期:2020-02-26 00:00:00