Reactivity of Haemophilus influenzae type b anti-pili antibodies.

Abstract:

:The reactivity of anti-pilus antibodies to native and denatured Haemophilus influenzae b (Hib) pili was studied using rabbit serum prepared against piliated H. influenzae b strain M43 (p+) and adsorbed with its non-piliated variant, strain M42 (p-). The specificity of the adsorbed serum for Hib pili was documented by immunogold electron microscopy and by immunoprecipitation, which revealed the 24 kDa pilin band from strain M43 (p+) that was not seen on strain M42 (p-). In immunodot assays, the anti-pilus antibodies reacted with the native pili present on the outer membrane of strain M43 (p+), but on Western blot assay using denatured outer membranes, the anti-pilus antibodies did not react with the 24 kDa pilin subunit. These data demonstrate that the anti-pilus antibodies in the adsorbed serum recognize conformational epitopes that depend on the tertiary or quaternary structure of Hib pili.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Gilsdorf JR,Forney LJ,LiPuma JJ

doi

10.1016/0882-4010(89)90049-1

subject

Has Abstract

pub_date

1989-10-01 00:00:00

pages

311-6

issue

4

eissn

0882-4010

issn

1096-1208

pii

0882-4010(89)90049-1

journal_volume

7

pub_type

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