Selective permissiveness of TPA differentiated THP-1 myelomonocytic cells for human cytomegalovirus strains AD169 and Towne.

Abstract:

:Two highly passaged laboratory strains of human cytomegalovirus (HCMV), AD169 and Towne, were tested for their ability to infect and replicate in THP-1 myelomonocytic cells differentiated with 12-O-tetradecanoylphorbol-13-acetate (TPA). TPA treatment of human THP-1 cells increased the number of cells that expressed HCMV immediate early (IE1) antigen from 0.06% prior to TPA treatment to 12% following cell differentiation. The Towne but not the AD169 strain replicated in differentiated THP-1 cells as determined by HCMV DNA replication and infectious virus production. Major early (HCMVTRL4) mRNA was present in both the abortive and productive THP-1 cell infections.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Turtinen LW,Seufzer BJ

doi

10.1006/mpat.1994.1037

subject

Has Abstract

pub_date

1994-05-01 00:00:00

pages

373-8

issue

5

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(84)71037-0

journal_volume

16

pub_type

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