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Identification of 60 secreted proteins for Mycoplasma bovis with secretome assay.

Abstract：

:Mycoplasma bovis is a risky pathogen mainly responsible for pneumonia and mastitis in cattle. Up to date, its pathogenesis is not clear. Since secreted proteins have a tricky role in M. bovis pathogenesis, this study was designed to systematically reveal M. bovis secretome and potential role in virulence of the pathogen. By using bioinformatics tools, a total of 246 secreted proteins were predicted based on M. bovis genome. Among them, 14 were classical, 154 non-classical and 78 both pathways. Then by using 2-dimensional gel electrophoresis (2-DE) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF- MS), 169 proteins were revealed. Of them, 60 were predicted to be secreted including 3 classical, 43 non-classical, and 14 both classical and non-classical. Further 8 proteins (MbovP0038, MbovP0338, MbovP0341, MbovP0520, MbovP0581, MbovP0674, MbovP0693, MbovP0845) were predicted to be virulence-related factors with VFDB. In addition, MbovP0581 (ABC transporter protein) was validated experimentally as secreted in nature and highly immunogenic reacting with sera of cattle experimentally infected with M. bovis. In conclusion, this study might be a crucial step towards a better understanding of pathogenesis and leading to the development of novel diagnostic marker and potent vaccine against M. bovis.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Zubair M,Muhamed SA,Khan FA,Zhao G,Menghwar H,Faisal M,Zhang H,Zhu X,Rasheed MA,Chen Y,Marawan MA,Chen H,Guo A

doi

10.1016/j.micpath.2020.104135

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

104135

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(20)30422-8

journal_volume

143

pub_type

杂志文章

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