Abstract:
:A variant of Vero toxin 2 (VT2), or Shiga-like toxin II (SLT-II) was purified from a cloned strain of Escherichia coli carrying a gene that encodes SLT-IIva, which is most closely related to SLT-IIv (VT2vp, VTe) in its nucleotide sequence. The purified SLT-IIva showed a slight difference from the purified SLT-IIv (VT2vp) in mobility on polyacrylamide gel disc electrophoresis and SDS-polyacrylamide gel slab electrophoresis and in pI, but both showed a similar potency in biological activities. Ouchterlony double diffusion test revealed that the purified SLT-IIva and SLT-IIv (VT2vp) formed a spur against anti-SLT-IIva and anti-SLT-IIv (VT2vp) antisera.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Ohmura M,Cao C,Karasawa T,Okuda J,Kurazono H,Gannon VP,Gyles CL,Takeda Ydoi
10.1006/mpat.1993.1089subject
Has Abstractpub_date
1993-11-01 00:00:00pages
399-405issue
5eissn
0882-4010issn
1096-1208pii
S0882-4010(83)71089-2journal_volume
15pub_type
杂志文章abstract::Diagnosis of toxoplasmosis is an important issue, especially in at-risk patients. The molecular methods showed a promising future for such diagnosis; however, the method itself and the target sequence to be detected is an important part of accurate detection of the infection. The aim of the present study was to evalua...
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journal_title:Microbial pathogenesis
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abstract:BACKGROUND:Tuberculosis (TB) remains a major public health problem. SH3RF1 and SH3RF2 are candidate genes with multiple single-nucleotide polymorphisms (SNPs) that have the potential to participate in Mycobacterium infection via activation of the JNK signaling pathway. In this case-control study, we aimed to investigat...
journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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更新日期:2018-10-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
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journal_title:Microbial pathogenesis
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doi:10.1016/j.micpath.2019.103693
更新日期:2019-11-01 00:00:00
abstract::Saffron (Crocus sativus L.) is an important plant in medicine. The Kashmir Valley (J&K, India) is one of the world's largest and finest saffron producing regions. However, over the past decade, there has been a strong declining trend in saffron production in this area. Plant Growth Promoting Rhizobacteria (PGPR) are f...
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journal_title:Microbial pathogenesis
pub_type: 杂志文章,meta分析
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更新日期:2012-08-01 00:00:00
abstract:OBJECTIVE:DNA vaccine has emerged as a promising approach with potential for Tuberculosis (TB) prevention in adults. However, the mechanism behind DNA vaccines is still largely unknown. MATERIALS AND METHODS:Utilizing the CRISPR/Cas9 technique, we engineered Ag85A mutated dendritic cells (Ag85A-M-DCs) in which the Ag8...
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journal_title:Microbial pathogenesis
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abstract::In the present study, we synthesized and characterized a probiotic Bacillus licheniformis cell free extract (BLCFE) coated silver nanoparticles (BLCFE-AgNPs). These BLCFE-AgNPs were characterized by UV-visible spectrophotometer, XRD, EDX, FTIR, TEM and AFM. A strong surface plasmon resonance centered at 422 nm in UV-v...
journal_title:Microbial pathogenesis
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doi:10.1016/j.micpath.2016.01.014
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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更新日期:2019-08-01 00:00:00
abstract::As one of the dominant bacteria in the ocean, Vibrio play important roles in maintaining the aquatic ecosystem. In this study, we studied the phylogenetic relationships of 32 Vibrio based on the 16S rRNA genes sequences and utilized substrate immersing zymography method to detect the trend of protease production and c...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.03.018
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abstract::Opportunistic infections, due to disease-related immunosuppression, constitute the major cause of death in American visceral leishmaniasis (AVL). Sera from these patients (AVL sera) non-specifically inhibit the in vitro proliferative response of normal human lymphocytes to lectins or antigens. In the present work, the...
journal_title:Microbial pathogenesis
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更新日期:2006-06-01 00:00:00