Purification and some properties of a Vero toxin 2 (Shiga-like toxin II) variant (SLT-IIva) of Escherichia coli isolated from infantile diarrhea.

Abstract:

:A variant of Vero toxin 2 (VT2), or Shiga-like toxin II (SLT-II) was purified from a cloned strain of Escherichia coli carrying a gene that encodes SLT-IIva, which is most closely related to SLT-IIv (VT2vp, VTe) in its nucleotide sequence. The purified SLT-IIva showed a slight difference from the purified SLT-IIv (VT2vp) in mobility on polyacrylamide gel disc electrophoresis and SDS-polyacrylamide gel slab electrophoresis and in pI, but both showed a similar potency in biological activities. Ouchterlony double diffusion test revealed that the purified SLT-IIva and SLT-IIv (VT2vp) formed a spur against anti-SLT-IIva and anti-SLT-IIv (VT2vp) antisera.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Ohmura M,Cao C,Karasawa T,Okuda J,Kurazono H,Gannon VP,Gyles CL,Takeda Y

doi

10.1006/mpat.1993.1089

subject

Has Abstract

pub_date

1993-11-01 00:00:00

pages

399-405

issue

5

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(83)71089-2

journal_volume

15

pub_type

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