Abstract:
:Porcine reproductive and respiratory syndrome (PRRS), characterized by respiratory disorders in piglets and reproductive failure in sows, is still the great threat of swine industry. Recently, Emergence of the novel NADC30-like PRRS viruses (PRRSVs) has caused widespread outbreaks of PRRS. To investigate the epidemic characteristics of PRRSVs in Central China since 2014, 6372 clinical serum samples were tested by ELISA, 250 tissue samples were tested by RT-PCR, and among these, 30 ORF5 and 17 Nsp2 genes sequences were analyzed. Phylogenetic tree based on ORF5 revealed that, 17 isolates were clustered into subgroup 1, represented by the NADC30. And for the Nsp2, The strains which had a discontinuous 131-amino-acid deletion in Nsp2, called NADC30-like strains, were clustered into subgroup 2. Our data suggested that the NADC30-like PRRSV strains spread quickly and are now circulating and prevalent in Central China as well as the classical HP-PRRSV strains. In addition, amino acid variation analysis of GP5 revealed that the amino acid sequences of NADC30-like PRRSV strains underwent rapid evolution and contained extensive amino acid substitutions in important motifs, such as potential neutralization epitope and the N-glycosylation sites. In summary, our data would provide a large amount of detailed information on molecular variation and genetic diversity of PRRSV in central China.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Wang LJ,Xie W,Chen XX,Qiao S,Zhao M,Gu Y,Zhao BL,Zhang Gdoi
10.1016/j.micpath.2017.05.021subject
Has Abstractpub_date
2017-08-01 00:00:00pages
20-28eissn
0882-4010issn
1096-1208pii
S0882-4010(16)30375-8journal_volume
109pub_type
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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更新日期:2014-11-01 00:00:00
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journal_title:Microbial pathogenesis
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更新日期:2018-01-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1998.0208
更新日期:1998-06-01 00:00:00
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doi:10.1016/j.micpath.2003.08.006
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journal_title:Microbial pathogenesis
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doi:10.1006/mpat.1996.0019
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journal_title:Microbial pathogenesis
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abstract::We have described a new class of live attenuated salmonella vaccines harbouring lesions in htrA, a stress protein gene previously. The virulence and invasiveness of Salmonella htrA mutants was investigated in three models of increased susceptibility to Salmonella infection. These included BALB/c mice, either given sub...
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doi:10.1016/0882-4010(92)90049-t
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