Abstract:
:Dendritic cells (DCs) are key linkages between innate immunity and acquired immunity. The antigens that promote the functions of DCs might be the effective candidates of novel vaccine. In this research, the ability of ubiquitin-conjugating enzyme (UCE), a recognized common antigens among chicken Eimeria species, to stimulate DCs of chickens were evaluated. We cloned UCE gene from Eimeria maxima (EmUCE), and its protein expression was confirmed by SDS-PAGE and western-blot. Immunofluorescence assay confirmed the binding of rEmUCE on the surface of chicken splenic-derived DCs (ChSP-DCs). Flow cytometric analysis showed that rEmUCE-treated ChSP-DCs increased MHCII, CD1.1, CD11c, CD80, and CD86 phenotypes. qRT-PCR indicated that transcript levels of maturation markers CCL5, CCR7, and CD83 in ChSP-DCs were upregulated in response to rEmUCE. Following rEmUCE treatment, chSP-DCs activated TLR signaling and inhibited Wnt signaling. Moreover, rEmUCE promoted DC-mediated T-cell proliferation in DC/T-cell co-incubation. Interestingly, CD3+/CD4+ T-cells were significantly enhanced when rEmUCE-treated chSP-DCs were co-incubated with T-cells. Cytokine secretion pattern of rEmUCE-stimulated ChSP-DCs revealed that the production of IL-12 and IFN-γ was increased whereas IL-10 and TGF-β were unchanged. Likewise, the co-incubation of ChSP-DCs with T-cells indicated increased production of IFN-γ but not IL-4. Collectively, rEmUCE could polarize DCs to immunogenic phenotype and shift the immune cells towards Th1 response. Our observations provide valuable insight for future research aimed at vaccine development against avian coccidiosis.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Lakho SA,Haseeb M,Huang J,Hasan MW,Naqvi MA,Zhou Z,Song X,Yan R,Xu L,Li Xdoi
10.1016/j.micpath.2020.104162subject
Has Abstractpub_date
2020-06-01 00:00:00pages
104162eissn
0882-4010issn
1096-1208pii
S0882-4010(20)30449-6journal_volume
143pub_type
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journal_title:Microbial pathogenesis
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doi:10.1006/mpat.1998.0269
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更新日期:2017-04-01 00:00:00
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doi:10.1016/j.micpath.2015.11.013
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doi:10.1016/j.micpath.2019.02.006
更新日期:2019-04-01 00:00:00
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