Abstract:
:Initial Enterococcus faecalis-endothelial cell molecular interactions which lead to enterococci associating in the host endothelial tissue, colonizing it and proliferating there can be assessed using in vitro models. Cultured human umbilical vein endothelial cells (HUVEC) have been used to study other Gram-positive bacteria-cell interactions; however, few studies have been aimed at establishing the relationship of E. faecalis with endothelial cells. The aggregation substance (AS) family of adhesins represents an E. faecalis virulence factor which has been implicated in endocarditis severity and bacterial persistence. The Asc10 protein (a member of this family) promotes bacterium-bacterium aggregation and bacterium-host cell binding. Evaluating Asc10 role in bacterial internalization by cultured enterocytes has shown that this adhesin facilitates E. faecalis endocytosis by HT-29 cells. A few eukaryotic cell structural components, such as cytoskeletal proteins, have been involved in E. faecalis entry into cell-lines; it is thus relevant to determine whether Asc10, as well as microtubules and actin microfilaments, play a role in E. faecalis internalization by cultured endothelial cells. The role of Asc10 and cytoskeleton proteins in E. faecalis ability to enter HUVEC was assessed in the present study, as well as cell apoptosis induction by enterococcal internalization by HUVEC; the data indicated increased cell apoptosis and that cytoskeleton components were partially involved in E. faecalis entry to endothelial cells, thereby suggesting that E. faecalis Asc10 protein would not be a critical factor for bacterial entry to cultured HUVEC.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Millán D,Chiriboga C,Patarroyo MA,Fontanilla MRdoi
10.1016/j.micpath.2012.11.007subject
Has Abstractpub_date
2013-04-01 00:00:00pages
62-9eissn
0882-4010issn
1096-1208pii
S0882-4010(12)00199-4journal_volume
57pub_type
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1999.0320
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journal_title:Microbial pathogenesis
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doi:10.1016/0882-4010(92)90049-t
更新日期:1992-04-01 00:00:00
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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更新日期:2013-12-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章,评审
doi:10.1016/j.micpath.2014.03.006
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1998.0265
更新日期:1999-04-01 00:00:00
abstract::Like most microorganisms, Neisseria gonorrhoeae alters gene expression in response to iron availability. The ferric uptake regulator Fur has been shown to be involved in controlling this response, but the extent of this involvement remains unknown. It is known that in addition to working directly to repress gene expre...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2008.12.003
更新日期:2009-03-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1994.1055
更新日期:1994-08-01 00:00:00
abstract::Two highly passaged laboratory strains of human cytomegalovirus (HCMV), AD169 and Towne, were tested for their ability to infect and replicate in THP-1 myelomonocytic cells differentiated with 12-O-tetradecanoylphorbol-13-acetate (TPA). TPA treatment of human THP-1 cells increased the number of cells that expressed HC...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1994.1037
更新日期:1994-05-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1999.0272
更新日期:1999-06-01 00:00:00
abstract::Pasteurella multocida capsular type A can cause a pulmonary infection, leading to serious pecuniary losses in cattle. The heterogeneity of infection outcome of P. multocida strains showing different virulence may be related to divergent expression of virulence genes. In this study, we compared the transcriptional resp...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.103911
更新日期:2020-03-01 00:00:00
abstract::Animals nurtured with a high-concentrate diet for a more extended period can cause subacute ruminal acidosis (SARA). In this study, twelve mid-lactating goats were separated into two groups (n = 6): a high concentrate diet (HC) control and a high concentrate with buffer (HCB) treatment group. Rumen fistula was install...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.05.045
更新日期:2018-09-01 00:00:00
abstract:BACKGROUND:Since the pre-transplant status affects the renal transplantation success and ultimately the survival rate, identifying the probable risk factors that increase the chance of BK virus replication in end-stage renal disease patients can be included in proposing proper surveillance guidelines during pre and pos...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104558
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abstract::In this study experimental mouse model for Chlamydia pneumoniae infection was used to elucidate the nature of immune response developing during primary and secondary infection. First we examined the mononuclear cells from different lymphoid organs in BALB/c mice during C. pneumoniae infection and detected a strong lym...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2009.01.006
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journal_title:Microbial pathogenesis
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doi:10.1006/mpat.1998.0211
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abstract::Moritella viscosa is the aetiological agent of winter-ulcer disease in farmed salmonids in the North Atlantic. Previously, two major (typical and variant) genetic clades have been demonstrated within this bacterial species, one of which is almost solely related to disease in Atlantic salmon (Salmo salar). In the prese...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2014.09.014
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journal_title:Microbial pathogenesis
pub_type: 杂志文章,评审
doi:10.1016/j.micpath.2018.08.008
更新日期:2018-10-01 00:00:00
abstract::The Sm-like protein Hfq has been implicated in the regulation of sigmaS-dependent and sigmaS-independent genes in E. coli and in the regulation of virulence factors in both, Yersinia enterocolitica and Brucella abortus. Here, we have studied the effect of Hfq on virulence and stress response of Pseudomonas aeruginosa ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/s0882-4010(03)00149-9
更新日期:2003-11-01 00:00:00
abstract::Toxoplasma gondii is one of the most successful intracellular protozoan parasites in the world, which can infect most warm-blooded animals including foxes in the world and cause toxoplasmosis. This is the first meta-analysis to assess the overall prevalence and potential risk factors of T. gondii among foxes in the wo...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104699
更新日期:2020-12-28 00:00:00
abstract::Acute lung injury (ALI) is considered as an uncontrolled inflammatory response that can leads to acute respiratory distress syndrome (ARDS), which limits the therapeutic strategies. Ginsenosides Rb1 (Rb1), an active ingredient obtained from Panax ginseng, possesses a broad range of pharmacological and medicinal proper...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104109
更新日期:2020-06-01 00:00:00
abstract::An inbred strain of the southern platyfish, Xiphophorus maculatus, was used as a host for Aeromonas hydrophila and Yersinia ruckeri infections. The infections were initiated by holding the platyfish in inoculation baths containing dilutions of virulent A. hydrophila or Y. ruckeri strains. Inoculating the platyfish in ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1996.0011
更新日期:1996-02-01 00:00:00
abstract::Influenza virus is responsible for significant morbidity and mortality worldwide. Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is the major cause of death in influenza virus infected patients. Recent studies indicated that active glucagon like peptide-1 (GLP-1) encoded by glucagon (GCG) gene exer...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104645
更新日期:2020-12-05 00:00:00
abstract::Pneumocystis infection leads to a life threatening pneumonia in susceptible individuals. While depletion or dysfunction of CD4+T cells is a key determinant of susceptibility to Pneumocystis, the host response that leads to resolution of infection or lung injury is less well understood. We had previously shown that mic...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2005.10.001
更新日期:2006-01-01 00:00:00
abstract::The genus Aeromonas comprises more than thirty Gram-negative bacterial species which mostly act as opportunistic microorganisms. These bacteria are distributed naturally in diverse aquatic ecosystems, where they are easily isolated from animals such as fish and crustaceans. A capacity for adaptation also makes Aeromon...
journal_title:Microbial pathogenesis
pub_type: 杂志文章,评审
doi:10.1016/j.micpath.2019.02.036
更新日期:2019-05-01 00:00:00