Abstract：
:Many virulence genes have been reported to play important roles in Helicobacter pylori pathogenesis. However the detailed mechanisms of many of them have not been completely clear. In this study, we found gene hp0169, encoding a putative collagenase (HpPrtC), was involved in pathogenesis of H. pylori. Recombinant HpPrtC shows activities to both native and heat-denatured collagens. This result indicated that HpPrtC may act as a virulence factor to help the bacterium colonize in their host stomach by degrading surrounding collagens. hp0169 was deleted by homologous recombination to study its function in bacterium-host cell interaction. For the pathogenic functions on the host cells, the hp0169 mutant exhibits no significant changes on inducing apoptosis of GES-1 cells. However, the viability and proliferation rate of GES-1 cells infected with mutant strain were higher than the cells infected with wild-type strain. These results indicated that except for its collagenolytic activity, HpPrtC might participate in H. pylori pathogenesis through an additional pathway. Functional studies on hp0169 involved in pathogenesis would shed light on deep understanding of the pathogenic mechanism of H. pylori.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Zhao H,Ji X,Chen X,Li J,Zhang Y,Du Z,Zhang Y,Li Bdoi
10.1016/j.micpath.2017.01.039subject
Has Abstractpub_date
2017-03-01 00:00:00pages
225-231eissn
0882-4010issn
1096-1208pii
S0882-4010(16)30801-4journal_volume
104pub_type
杂志文章abstract：:In mice, nitric oxide (NO) production by inducible NO synthase (iNOS), is a component of the control of Brucella infection. In humans, the involvement of iNOS in infection is still a matter of debate. Based on in vitro experiments, it was recently postulated that in humans, Brucella infection tends to become chronic b...
journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
pub_type： 杂志文章,评审
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2016.10.002
更新日期：2016-11-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.07.021
更新日期：2017-09-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章,评审
doi：10.1016/j.micpath.2018.07.021
更新日期：2018-10-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2011.03.004
更新日期：2011-07-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2017.09.034
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2019.01.003
更新日期：2019-03-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1998.0226
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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更新日期：2020-12-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2015.12.012
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2015.11.013
更新日期：2016-02-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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更新日期：2016-09-01 00:00:00
abstract：:Carbohydrate analyses of whole-spore extracts have confirmed the presence of rhamnose in the spore of the fully virulent Ames strain of Bacillus anthracis. A gene cluster containing loci with high homology to the rhamnose biosynthetic genes, rmlACBD, was identified within the B. anthracis chromosome. The first gene of...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2004.10.001
更新日期：2005-01-01 00:00:00
abstract：:Staphylococcus aureus is a bacterial pathogen causing approximately 80% of all cases of human osteomyelitis. This bacterium can adhere to and become internalized by osteoblasts and previous studies indicate that osteoblasts are active in the internalization process. In the current study, we examined the roles of micro...
journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1999.0272
更新日期：1999-06-01 00:00:00
