Functional study of gene hp0169 in Helicobacter pylori pathogenesis.


:Many virulence genes have been reported to play important roles in Helicobacter pylori pathogenesis. However the detailed mechanisms of many of them have not been completely clear. In this study, we found gene hp0169, encoding a putative collagenase (HpPrtC), was involved in pathogenesis of H. pylori. Recombinant HpPrtC shows activities to both native and heat-denatured collagens. This result indicated that HpPrtC may act as a virulence factor to help the bacterium colonize in their host stomach by degrading surrounding collagens. hp0169 was deleted by homologous recombination to study its function in bacterium-host cell interaction. For the pathogenic functions on the host cells, the hp0169 mutant exhibits no significant changes on inducing apoptosis of GES-1 cells. However, the viability and proliferation rate of GES-1 cells infected with mutant strain were higher than the cells infected with wild-type strain. These results indicated that except for its collagenolytic activity, HpPrtC might participate in H. pylori pathogenesis through an additional pathway. Functional studies on hp0169 involved in pathogenesis would shed light on deep understanding of the pathogenic mechanism of H. pylori.


Microb Pathog


Microbial pathogenesis


Zhao H,Ji X,Chen X,Li J,Zhang Y,Du Z,Zhang Y,Li B




Has Abstract


2017-03-01 00:00:00












  • Identification of a maltose-inducible major outer membrane protein in Actinobacillus (Haemophilus) pleuropneumoniae.

    abstract::The addition of maltose to the growth media of Actinobacillus pleuropneumonia (serotype 1) resulted in the induction of an outer membrane protein (OMP) with a molecular mass of 42 kDa. This protein had porin-like properties in that it was peptidoglycan-associated and was resistant to proteolysis by trypsin. A pleuropn...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Deneer HG,Potter AA

    更新日期:1989-06-01 00:00:00

  • Intracellular signalling and cytoskeletal rearrangement involved in Yersinia pestis plasminogen activator (Pla) mediated HeLa cell invasion.

    abstract::Yersinia pestis, the etiologic agent of plague is a highly invasive organism being able to invade non-phagocytic epithelial cells. Its plasminogen activator (Pla), encoded by the pPCP1 plasmid plays a pivotal role in internalisation of bacteria by HeLa cells. The aim of this study was to analyse the intracellular sign...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Benedek O,Nagy G,Emody L

    更新日期:2004-07-01 00:00:00

  • Biofilm inhibitory effect of chlorhexidine conjugated gold nanoparticles against Klebsiella pneumoniae.

    abstract::Klebsiella pneumoniae (K. pneumoniae) is one of the major pathogen associated with nosocomial infections, especially catheter associated urinary tract infections which involved biofilm formation. This study was designed to evaluate the antibiofilm efficacy of gold nanoparticle conjugated with chlorhexidine (Au-CHX) ag...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Ahmed A,Khan AK,Anwar A,Ali SA,Shah MR

    更新日期:2016-09-01 00:00:00

  • Comparative meta-analysis of the effect of Lactobacillus species on weight gain in humans and animals.

    abstract:BACKGROUND:Obesity is associated with alteration of the gut microbiota. In order to clarify the effect of Lactobacillus-containing probiotics (LCP) on weight we performed a meta-analysis of clinical studies and experimental models. We intended to assess effects by Lactobacillus species. METHODS:A broad search with no ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,meta分析


    authors: Million M,Angelakis E,Paul M,Armougom F,Leibovici L,Raoult D

    更新日期:2012-08-01 00:00:00

  • Alterations in metabolic pathways in gastric epithelial cells infected with Helicobacter pylori.

    abstract::Helicobacter pylori (H. pylori), which is a spiral-shaped Gram-negative microaerobic bacterium, is a causative pathogen. The entry of H. pylori into gastric epithelial cells involves various host signal transduction events, and its virulence factors can also cause a variety of biological responses. In this study, AGS ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Matsunaga S,Nishiumi S,Tagawa R,Yoshida M

    更新日期:2018-11-01 00:00:00

  • Antimicrobial and antibiofilm activity of the benzoquinone oncocalyxone A.

    abstract::Resistance to antimicrobials is a challenging issue that complicates the treatment of infections caused by bacteria and fungi, thus requiring new therapeutic options. Oncocalyxone A, a benzoquinone obtained from Auxemma oncocalyx (Allem) Taub has several biological effects; however, there is no data on its antimicrobi...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: da Silva RE,Ribeiro FOS,de Carvalho AMA,Daboit TC,Marinho-Filho JDB,Matos TS,Pessoa ODL,de Souza de Almeida Leite JR,de Araújo AR,Dos Santos Soares MJ

    更新日期:2020-12-01 00:00:00

  • The genus Aeromonas: A general approach.

    abstract::The genus Aeromonas comprises more than thirty Gram-negative bacterial species which mostly act as opportunistic microorganisms. These bacteria are distributed naturally in diverse aquatic ecosystems, where they are easily isolated from animals such as fish and crustaceans. A capacity for adaptation also makes Aeromon...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审


    authors: Gonçalves Pessoa RB,de Oliveira WF,Marques DSC,Dos Santos Correia MT,de Carvalho EVMM,Coelho LCBB

    更新日期:2019-05-01 00:00:00

  • Immunopathogenesis of infection with the visceralizing Leishmania species.

    abstract::Human leishmaniasis is a spectral disease that includes asymptomatic self-resolving infection, localized skin lesions, and progressive visceral leishmaniasis. With some overlap, visceral and cutaneous leishmaniasis are usually caused by different species of Leishmania. This review focuses on host responses to infectio...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审


    authors: Wilson ME,Jeronimo SM,Pearson RD

    更新日期:2005-04-01 00:00:00

  • Isolation and characterization of a broad-spectrum phage of multiple drug resistant Salmonella and its therapeutic utility in mice.

    abstract::Salmonella are causes of livestock, poultry, and other animal diseases but they also have the potential to infect people. Currently, antibiotics are the first choice for treatment of Salmonella infections. Thus, the utility of phage has become the research focus for scientists for several reasons. There are efficient,...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Tang F,Zhang P,Zhang Q,Xue F,Ren J,Sun J,Qu Z,Zhuge X,Li D,Wang J,Jiang M,Dai J

    更新日期:2019-01-01 00:00:00

  • Nutritional interference for phenotypic biofilm quantification in Aeromonas spp. isolates containing the fla gene.

    abstract::The Aeromonas genus has several virulence factors associated with the development of diseases in aquatic organisms, leading to losses in aquaculture. One of these factors is the flagella's formation which allows the biofilm's formation that provides the microorganisms a greater pathogenicity, greater protection to cer...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Freire NB,Magalhães TC,Nunes Soares RA,da Costa MM,Gouveia GV

    更新日期:2019-02-01 00:00:00

  • Antibacterial mechanisms of cinnamon and its constituents: A review.

    abstract:BACKGROUND:In the current healthcare environment, an alarming rise in multi-drug resistant bacterial infections has led to a global health threat. The lack of new antibiotics has created a need for developing alternative strategies. OBJECTIVE:Understanding the antibacterial mechanisms of cinnamon and its constituents ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审


    authors: Vasconcelos NG,Croda J,Simionatto S

    更新日期:2018-07-01 00:00:00

  • The role of O1-antigen in the adhesion to uroepithelial cells of Klebsiella pneumoniae grown in urine.

    abstract::We obtained mutants devoid of the O1-antigen, the capsular polysaccharide (K antigen) or both from Klebsiella pneumoniae clinical isolates (urinary infection). These mutants were grown in urine, and their ability to fimbriate and to adhere were studied. Mutants lacking the O1-antigen, independently of the other surfac...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Merino S,Rubires X,Aguilar A,Tomás JM

    更新日期:1997-07-01 00:00:00

  • Lactobacillus acidophilus attenuates toxin production by Vibrio cholerae and shigella dysenteriae following intestinal epithelial cells infection.

    abstract:AIMS:The main objective of the present study was to assess and compare the safety and inhibitory efficacy of Lactobacillus acidophilus against cholera toxin and shigatoxin production by measuring CTX-B and Stx1 expression level in Caco-2 cells exposed to Vibrio cholerae (as a non-invasive small intestine pathogens and ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Alamdary SZ,Bakhshi B

    更新日期:2020-12-01 00:00:00

  • Identification and expression of nor efflux family genes in Staphylococcus epidermidis that act against gatifloxacin.

    abstract::NorA, NorB, and NorC are efflux proteins in the Nor family that regulate the secretion of fluoroquinolones, and MgrA/NorR is a transcription factor of the Nor family. Overexpression of Nor family proteins provides fluoroquinolone resistance in Staphylococcus aureus. However, in coagulase-negative staphylococci (CNS), ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Juárez-Verdayes MA,Parra-Ortega B,Hernández-Rodríguez C,Betanzos-Cabrera G,Rodríguez-Martínez S,Cancino-Diaz ME,Cancino-Diaz JC

    更新日期:2012-06-01 00:00:00

  • Complete genome sequence of Janthinobacterium sp. B9-8, a violacein-producing bacterium isolated from low-temperature sewage.

    abstract::Janthinobacterium sp. B9-8, isolated from low temperature-sewage in Xinjiang, China, is capable of producing violacein, a promising antibiotic. Here we report the genome sequence of B9-8, which consist of 4,726,850 bp with a G + C content of 48.72%. The violacein biosynthesis gene cluster vioABCDE was identified and a...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Xu X,Tian L,Zhang S,Jiang L,Zhang Z,Huang H

    更新日期:2019-03-01 00:00:00

  • Expression and sequence diversity of the complement regulating outer surface protein E in Borrelia afzelii vs. Borrelia garinii in patients with erythema migrans or neuroborreliosis.

    abstract::Outer surface protein E (OspE) is a complement factor H-binding virulence factor of borrelial subspecies. It is usually absent from in vitro grown Borrelia garinii, although in vivo B. garinii causes neuroborreliosis (NB). We analyzed the presence and sequence spectrum of the ospE genes in vivo in Borrelia spirochetes...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Panelius J,Ranki A,Meri T,Seppälä I,Meri S

    更新日期:2010-12-01 00:00:00

  • Entry and intracellular replication of Mycobacterium tuberculosis in cultured human microvascular endothelial cells.

    abstract::Establishment of pulmonary Mycobacterium tuberculosis infection requires evasion of host innate defenses. In the lung alveoli, epithelial cells naturally resist uptake by the inhaled bacilli while macrophages patrol the epithelial surface and phagocytose foreign microbes. Alveolar microvascular endothelial cells, howe...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Mehta PK,Karls RK,White EH,Ades EW,Quinn FD

    更新日期:2006-08-01 00:00:00

  • Fis is required for proper regulation of ssaG expression in Salmonella enterica serovar Typhimurium.

    abstract::Salmonella pathogenicity island 2 (SPI2) encodes a type III secretion system (TTSS) necessary for bacterial survival and replication in intracellular environment of host cells. SPI2 genes are transcribed preferentially after Salmonella enters the host cells. Transcriptional regulation of ssaG encoding the component of...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Lim S,Kim B,Choi HS,Lee Y,Ryu S

    更新日期:2006-07-01 00:00:00

  • Porcine 2', 5'-oligoadenylate synthetase 2 inhibits porcine reproductive and respiratory syndrome virus replication in vitro.

    abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) is acknowledged a fulminating infectious pathogen affecting the pig farming industry, and current vaccines and drugs could hardly inhibit this virus. The 2', 5'-oligoadenylate synthetase (OASs) have antiviral activities, but the role(s) played by porcine OAS2...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Zhao M,Wan B,Li H,He J,Chen X,Wang L,Wang Y,Xie S,Qiao S,Zhang G

    更新日期:2017-10-01 00:00:00

  • Acyl homoserine lactone changes the abundance of proteins and the levels of organic acids associated with stationary phase in Salmonella Enteritidis.

    abstract::Quorum sensing (QS) is cell-cell communication mechanism mediated by signaling molecules known as autoinducers (AIs) that lead to differential gene expression. Salmonella is unable to synthesize the AI-1 acyl homoserine lactone (AHL), but is able to recognize AHLs produced by other microorganisms through SdiA protein....

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: de Almeida FA,Pimentel-Filho NJ,Carrijo LC,Bento CBP,Baracat-Pereira MC,Pinto UM,de Oliveira LL,Vanetti MCD

    更新日期:2017-01-01 00:00:00

  • Transforming growth factor beta (TGF-b1) plays a detrimental role in the progression of experimental Mycobacterium avium infection; in vivo and in vitro evidence.

    abstract::BALB/c mice were infected with 10(5) colony forming units (cfu) of Mycobacterium avium TMC 702 i.v. and the growth of the inoculum followed in the spleens of control mice. Other infected mice given weekly doses of 1 microgram of TGF-b1 or weekly doses of 2 mg of a rabbit antiserum against mouse TGF-b1 were evaluated f...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Denis M,Ghadirian E

    更新日期:1991-11-01 00:00:00

  • Binding of plasma fibronectin to Candida albicans occurs through the cell binding domain.

    abstract::Candida albicans yeast cells bind soluble human plasma fibronectin (Fn) through a glycoprotein receptor (adhesin) located on the cell surface. This work demonstrates that a 120 kDa proteolytic fragment of Fn encompassing the cell binding domain binds more avidly to the yeast cell adhesin than does the parent Fn molecu...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Penn C,Klotz SA

    更新日期:1994-12-01 00:00:00

  • Identification of Bordetella avium using the polymerase chain reaction.

    abstract::A DNA fragment from Bordetella pertussis, encoding the fim2 fimbrial subunit gene with adjacent sequences, was used as a probe for the detection of homologous sequences in chromosomal DNA of Bordetella avium. A 1.8 kb Sa1I-PstI fragment from the genome of B. avium, which hybridized with the probe, was isolated and seq...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Savelkoul PH,de Groot LE,Boersma C,Livey I,Duggleby CJ,van der Zeijst BA,Gaastra W

    更新日期:1993-09-01 00:00:00

  • Interest of bacterial pangenome analyses in clinical microbiology.

    abstract::Thanks to the progress and decreasing costs in genome sequencing technologies, more than 250,000 bacterial genomes are currently available in public databases, covering most, if not all, of the major human-associated phylogenetic groups of these microorganisms, pathogenic or not. In addition, for many of them, sequenc...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Anani H,Zgheib R,Hasni I,Raoult D,Fournier PE

    更新日期:2020-12-01 00:00:00

  • Effect of immunosuppression on pathogenesis of peste des petits ruminants (PPR) virus infection in goats.

    abstract::In this study an attempt to address the effects of immunosuppression on pathogenesis of peste des petits ruminants (PPR) virus infection was undertaken. Cyclophosphamide and dexamethasone were used to immunosuppress the animals. The drug treated animals exhibited severe leukopaenia and lymphopaenia; one of the indicat...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Jagtap SP,Rajak KK,Garg UK,Sen A,Bhanuprakash V,Sudhakar SB,Balamurugan V,Patel A,Ahuja A,Singh RK,Vanamayya PR

    更新日期:2012-04-01 00:00:00

  • Structural basis for isoniazid resistance in KatG double mutants of Mycobacterium tuberculosis.

    abstract::The failure of drugs for effective treatment against infectious diseases can be attributed to resistant forms of causative agents. The evasive nature of Mycobacterium tuberculosis is partly associated to its physical features, such as having a thick cell wall and incorporation of beneficial mutations leading to drug r...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Rabha A,Singh A,Grover S,Kumari A,Pandey B,Grover A

    更新日期:2019-04-01 00:00:00

  • Staphylococcal chromosomal cassettes mec (SCCmec): A mobile genetic element in methicillin-resistant Staphylococcus aureus.

    abstract::Considered to be a potential "superbug", methicillin-resistant Staphylococcus aureus (MRSA) has been one of the major recent infectious pathogens and thus poses a challenge to hospital infection control. The mobile genetic element staphylococcal chromosomal cassette mec (SCCmec) carries both the mecA or mecC gene, enc...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审


    authors: Liu J,Chen D,Peters BM,Li L,Li B,Xu Z,Shirliff ME

    更新日期:2016-12-01 00:00:00

  • Microbial balance in the intestinal microbiota and its association with diabetes, obesity and allergic disease.

    abstract::Recent studies have been considered to symbiotic interactions of the human gastrointestinal microbiota and human lifestyle-related disorders. The human gastrointestinal microbiota continuously stimulates the immune system against opportunistic and pathogen bacteria from infancy. Changes in gastrointestinal microbiota ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审


    authors: Gholizadeh P,Mahallei M,Pormohammad A,Varshochi M,Ganbarov K,Zeinalzadeh E,Yousefi B,Bastami M,Tanomand A,Mahmood SS,Yousefi M,Asgharzadeh M,Kafil HS

    更新日期:2019-02-01 00:00:00

  • Serum dependent expression of Enterococcus faecalis adhesins involved in the colonization of heart cells.

    abstract::Our previous studies have shown that the adhesive ability of Enterococcus faecalis is dependent on the strain and is further modified by growth in serum. The data reported here demonstrate that E. faecalis adherence is mediated by carbohydrate residues present on the bacterial cell surface. Some of these (D-mannose an...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Guzmàn CA,Pruzzo C,Platè M,Guardati MC,Calegari L

    更新日期:1991-12-01 00:00:00

  • Production of di-(2-ethylhexyl) phthalate by Bacillus subtilis AD35: Isolation, purification, characterization and biological activities.

    abstract::The emergence of extensive antibiotics resistant bacteria increased the demands for finding out new sources of antimicrobial agents. Marine niches were reported to be rich in many competent producers of significant bioactive compounds. On the course of screening program for new antimicrobials, a Bacillus strain was is...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章


    authors: Lotfy WA,Mostafa SW,Adel AA,Ghanem KM

    更新日期:2018-11-01 00:00:00