Abstract:
:The essential oil from leaves of Croton grewioides Baill was obtained by hydrodistillation using Clevenger apparatus, and its chemical composition was analyzed by GC-MS, where 18 compounds were identified, mostly as monoterpenes (55.56%) and sesquiterpenes (44.44%), in which the major constituent was the α-pinene (47.43%). The essential oil of Croton grewioides (EOCg) and its major compound (α-pinene) were evaluated as modulators of antibiotic resistance in strain SA-1199B and IS-58 of Staphylococcus aureus that overexpresses efflux protein. The minimum inhibitory concentrations (MICs) of the antibiotics were determined by the microdilution assay in the absence and in the presence of sub-inhibitory concentration of EOCg and α-pinene. Although the EOCg and α-pinene did not indicate relevant antibacterial activity in vitro, they acted as antibiotic resistance modulators, i.e., EOCg in combination with norfloxacin, reducted its MIC, by 64× whereas in combination with tetracycline it was observed a reduction of 4×. Additionally, it was observed a MIC reduction of tetracycline by 32×, when combined with α-pinene. The results suggest that EOCg and α-pinene modulate or even reverse bacterial resistance as a putative efflux pump inhibitor.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
de Medeiros VM,do Nascimento YM,Souto AL,Madeiro SAL,Costa VCO,Silva SMPM,Falcão Silva VDS,Agra MF,de Siqueira-Júnior JP,Tavares JFdoi
10.1016/j.micpath.2017.09.034subject
Has Abstractpub_date
2017-10-01 00:00:00pages
468-471eissn
0882-4010issn
1096-1208pii
S0882-4010(17)30066-9journal_volume
111pub_type
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