Expression and sequence diversity of the complement regulating outer surface protein E in Borrelia afzelii vs. Borrelia garinii in patients with erythema migrans or neuroborreliosis.

Abstract:

:Outer surface protein E (OspE) is a complement factor H-binding virulence factor of borrelial subspecies. It is usually absent from in vitro grown Borrelia garinii, although in vivo B. garinii causes neuroborreliosis (NB). We analyzed the presence and sequence spectrum of the ospE genes in vivo in Borrelia spirochetes. DNA samples from the skin, serum and cerebrospinal fluid (CSF) of patients with infections caused by Borrelia afzelii or B. garinii were studied, and anti-OspE antibodies in the corresponding patient sera were detected by IgG ELISA using recombinant OspE as an antigen. ospE genes were found in 20 of 23 erythema migrans (EM) skin biopsies with B. afzelii, in 2 EM skin biopsies with unknown underlying subspecies, in 5 of 9 EM biopsies with B. garinii, and in 1 of 4 CSF samples of NB patients with B. garinii infection. All OspE sequences from B. garinii samples were identical. In contrast, OspE of B. afzelii origin showed more variation. Anti-OspE antibodies were found in 8/21 (38.0%) sera from patients with B. afzelii-associated EM. In conclusion, our results indicate that all borrelial subspecies, but not necessarily all strains, causing human infections can carry ospE genes to protect themselves against complement attack in vivo.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Panelius J,Ranki A,Meri T,Seppälä I,Meri S

doi

10.1016/j.micpath.2010.06.006

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

363-8

issue

6

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(10)00108-7

journal_volume

49

pub_type

杂志文章
  • Complete genome sequence of Janthinobacterium sp. B9-8, a violacein-producing bacterium isolated from low-temperature sewage.

    abstract::Janthinobacterium sp. B9-8, isolated from low temperature-sewage in Xinjiang, China, is capable of producing violacein, a promising antibiotic. Here we report the genome sequence of B9-8, which consist of 4,726,850 bp with a G + C content of 48.72%. The violacein biosynthesis gene cluster vioABCDE was identified and a...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2019.01.003

    authors: Xu X,Tian L,Zhang S,Jiang L,Zhang Z,Huang H

    更新日期:2019-03-01 00:00:00

  • Identification and expression of nor efflux family genes in Staphylococcus epidermidis that act against gatifloxacin.

    abstract::NorA, NorB, and NorC are efflux proteins in the Nor family that regulate the secretion of fluoroquinolones, and MgrA/NorR is a transcription factor of the Nor family. Overexpression of Nor family proteins provides fluoroquinolone resistance in Staphylococcus aureus. However, in coagulase-negative staphylococci (CNS), ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2012.03.001

    authors: Juárez-Verdayes MA,Parra-Ortega B,Hernández-Rodríguez C,Betanzos-Cabrera G,Rodríguez-Martínez S,Cancino-Diaz ME,Cancino-Diaz JC

    更新日期:2012-06-01 00:00:00

  • Principle component analysis (PCA) for investigation of relationship between population dynamics of microbial pathogenesis, chemical and sensory characteristics in beef slices containing Tarragon essential oil.

    abstract::Principle component analysis (PCA) was employed to examine the effect of the exerted treatments on the beef shelf life as well as discovering the correlations between the studied responses. Considering the variability of the dimensions of the responses, correlation coefficients were applied to form the matrix and extr...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2017.02.013

    authors: Alizadeh Behbahani B,Tabatabaei Yazdi F,Shahidi F,Mortazavi SA,Mohebbi M

    更新日期:2017-04-01 00:00:00

  • Differences in the carriage and the ability to utilize the serotype associated virulence plasmid in strains of Salmonella enterica serotype Typhimurium investigated by use of a self-transferable virulence plasmid, pOG669.

    abstract::Most strains of Salmonella enterica subspecies enterica serotype typhimurium (S. typhimurium) naturally harbour a virulence plasmid which carries the salmonella plasmid virulence (spv) genes. However, isolates belonging to certain phage types are generally found without the plasmid. We have utilized a self-transferabl...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2004.02.005

    authors: Olsen JE,Brown DJ,Thomsen LE,Platt DJ,Chadfield MS

    更新日期:2004-06-01 00:00:00

  • Presence of F-like OriT base-pair sequence on the virulence plasmids of Salmonella serovars Gallinarum, Enteritidis, and Typhimurium, but absent in those of Choleraesuis and Dublin.

    abstract::The 90 kb virulence plasmids of Salmonella biosers Gallinarum and Pullorum (expressed as serovar Gallinarum) are non-conjugative. They were, however, found to be readily mobilized by IncFI and IncFIV plasmids, but not by conjugative IncA, IncB = C, IncH, IncL and IncM R plasmids. No virulence plasmids of serovars Chol...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1994.1048

    authors: Ou JT,Lin MY,Chao HL

    更新日期:1994-07-01 00:00:00

  • Chlamydia pneumoniae facilitates monocyte adhesion to endothelial and smooth muscle cells.

    abstract::Chlamydia pneumoniae has been linked to atherosclerotic heart disease. However, there is a limited knowledge by which C. pneumoniae gain access to atheromatous lesions. The adhesion of C. pneumoniae -infected circulatory component(s) to endothelium and smooth muscle cells represents the first step in an inflammatory r...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.2000.0420

    authors: Kaul R,Wenman WM

    更新日期:2001-03-01 00:00:00

  • Lack of association between hemolysin production and acute inflammation in human urinary tract infection.

    abstract::Hemolysins are cytolytic proteins which have been extensively characterized at the molecular level, however, their in vivo functions remain unclear. This study analyzed the association of hemolysin production with the inflammatory response in patients with urinary tract infection (UTI). Infants and children with their...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1993.1045

    authors: Connell H,de Man P,Jodal U,Lincoln K,Svanborg C

    更新日期:1993-06-01 00:00:00

  • The role of fimbriae of uropathogenic Escherichia coli as carriers of the adhesin involved in mannose-resistant hemagglutination.

    abstract::The gene clusters encoding various P-fimbriae (F7(1), F7(2), F9 and F11) were compared. Deletion plasmids that lack the gene encoding the fimbrillin were derived from these gene clusters. Introduction of these deletion plasmids into an E. coli K12 strain resulted in non-fimbriated cells that still showed mannose-resis...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(86)90031-8

    authors: van Die I,Zuidweg E,Hoekstra W,Bergmans H

    更新日期:1986-02-01 00:00:00

  • Virulence-related genes, adhesion and invasion of some Yersinia enterocolitica-like strains suggests its pathogenic potential.

    abstract::Yersina enterocolitica-like species have not been extensively studied regarding its pathogenic potential. This work aimed to assess the pathogenic potential of some Y. enterocolitica-like strains by evaluating the presence of virulence-related genes by PCR and their ability to adhere to and invade Caco-2 and HEp-2 cel...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2017.01.008

    authors: Imori PF,Passaglia J,Souza RA,Rocha LB,Falcão JP

    更新日期:2017-03-01 00:00:00

  • Molecular characterization of a trans-acting, positive effector (ipaR) of invasion plasmid antigen synthesis in Shigella flexneri serotype 5.

    abstract::A trans-acting, positive effector of invasion plasmid antigen (Ipa) synthesis has been identified and mapped on the pWR100 invasion plasmid of Shigella flexneri serotype 5 (strain M90T-W). Recombinant plasmids carrying this regulatory gene, designated ipaR, were found to restore full virulence to a non-invasive ipaR::...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(90)90047-t

    authors: Buysse JM,Venkatesan M,Mills JA,Oaks EV

    更新日期:1990-03-01 00:00:00

  • Binding of Porphyromonas gingivalis gingipains to human CD4(+) T cells preferentially down-regulates surface CD2 and CD4 with little affect on co-stimulatory molecule expression.

    abstract::The role of Porphyromonas gingivalis cysteine proteinases (gingipains) in the evasion of host cell-mediated immunity has not been fully determined. In this study, modulation by gingipains of accessory and co-stimulatory molecule expression on human CD4(+) T cells was evaluated. Arg-gingipain rather than Lys-gingipain ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2005.01.001

    authors: Yun PL,Decarlo AA,Chapple CC,Collyer CA,Hunter N

    更新日期:2005-02-01 00:00:00

  • Role of H-2 and non-H-2-related genes in mouse susceptibility to Chlamydia psittaci.

    abstract::The genetic control of mouse susceptibility to Chlamydia psittaci was investigated after intravenous inoculation of 2 x 10(5) plaque-forming units (pfu). Splenic counts 6 days after inoculation gave the level of susceptibility. Results from inoculation of mice from H-2 congenic strains with three different genetic bac...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1994.1023

    authors: Buzoni-Gatel D,Bernard F,Pla M,Rodolakis A,Lantier F

    更新日期:1994-03-01 00:00:00

  • The multi-faceted potential of plant-derived metabolites as antimicrobial agents against multidrug-resistant pathogens.

    abstract::Multidrug-resistant (MDR) pathogens are currently causing serious problems globally in the medical setting. Improper and extensive usage of antibiotics results in a selective pressure supporting the rise of antibiotic-resistant microbes. Many key cellular bacterial components, including enzymes and small noncoding RNA...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审

    doi:10.1016/j.micpath.2018.01.043

    authors: Shin J,Prabhakaran VS,Kim KS

    更新日期:2018-03-01 00:00:00

  • Activated mouse macrophages kill Entamoeba histolytica trophozoites by releasing reactive nitrogen intermediates.

    abstract::Mouse macrophages activated by gamma interferon (IFN-gamma) and bacterial lipopolysaccharide (LPS) are highly cytotoxic for the enteric protozoan parasite Entamoeba histolytica. Herein, we show that this killing by activated macrophages is L-arginine dependent, inasmuch as it was blocked by exogenous arginase or NG-mo...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(92)90053-q

    authors: Denis M,Ghadirian E

    更新日期:1992-03-01 00:00:00

  • Recombinant Brugia malayi pepsin inhibitor (rBm33) induced monocyte function and absence of apoptotic cell death: an in vitro study.

    abstract::The effect of recombinant Brugia malayi pepsin inhibitor (rBm33) on human monocytes/macrophages has been examined using THP-1 cells. THP-1 cells stimulated with rBm33 showed enhanced levels of expression of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6) and diminished levels of IL-12, iNOS and anti-inflammatory cytok...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2012.03.009

    authors: Sreenivas K,Vijayan K,Babu S,Narayanan RB

    更新日期:2012-07-01 00:00:00

  • Integrons in uropathogenic Escherichia coli and their relationship with phylogeny and virulence.

    abstract::Uropathogenic Escherichia coli (UPEC) comprise a heterogeneous group of strains. In a previous epidemiological survey performed on 230 UPEC isolates, five virulence profiles were described, each one defined by the presence of some virulence determinants and by the absence of others. Phylogenetic groups and antibiotic ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2014.11.002

    authors: Poey ME,Laviña M

    更新日期:2014-12-01 00:00:00

  • Receptor affinity, stability and binding mode of Shiga toxins are determinants of toxicity.

    abstract::The closely related Shiga toxins, Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2), can bind to Gb3 receptors. However, Stx2-producing enterohemorrhagic Escherichia coli (EHEC) strains are more commonly associated with serious human disease (viz., hemolytic-uremic syndrome) than Stx1-producing strains. To clarify the rel...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2007.04.003

    authors: Shimizu T,Sato T,Kawakami S,Ohta T,Noda M,Hamabata T

    更新日期:2007-08-01 00:00:00

  • Potential of Bacillus amyloliquefaciens for biocontrol of bacterial canker of tomato incited by Clavibacter michiganensis ssp. michiganensis.

    abstract::A total of 150 rhizobacteria and endorhizobacteria previously isolated from three different horticultural crops; strawberry, apple and apricot were screened for antagonistic activitiy against Clavibacter michiganensis ssp. michiganensis. Among them strain S1, exhibiting significantly higher antagonistic and plant grow...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2019.03.006

    authors: Gautam S,Chauhan A,Sharma R,Sehgal R,Shirkot CK

    更新日期:2019-05-01 00:00:00

  • Transcriptional regulation of Yersinia pestis biofilm formation.

    abstract::Yersinia pestis, the causative agent of plague, is transmitted primarily by infected fleas in nature. Y. pestis can produce biofilms that block flea's proventriculus and promote flea-borne transmission. Transcriptional regulation of Y. pestis biofilm formation plays an important role in the response to complex changes...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章,评审

    doi:10.1016/j.micpath.2019.04.011

    authors: Liu L,Zheng S

    更新日期:2019-06-01 00:00:00

  • Systematic expression profiling analysis mines dys-regulated modules in active tuberculosis based on re-weighted protein-protein interaction network and attract algorithm.

    abstract::About 90% of tuberculosis (TB) patients latently infected with Mycobacterium tuberculosis (Mtb) show no symptoms, yet have a 10% chance in lifetime to progress active TB. Nevertheless, current diagnosis approaches need improvement in efficiency and sensitivity. The objective of this work was to detect potential signat...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2017.03.013

    authors: Sun Y,Weng Y,Zhang Y,Yan X,Guo L,Wang J,Song X,Yuan Y,Chang FY,Wang CL

    更新日期:2017-06-01 00:00:00

  • In silico design and in vitro validation of a novel PCR-RFLP assay for determination of phylogenetic clusters of streptokinase gene alleles in streptococci groups.

    abstract::Streptokinase (SK), a heterogeneous plasminogen (Pg) activator protein secreted by groups A, C and G streptococci (GAS/GCS/GGS) is a virulence factor composed of three structural domains; SKα/SKβ/SKγ. Phylogenetic analysis of the major variable region of SKβ (sk-V1; nucleotides 448-791; 343bp) which classifies the SK ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2019.103862

    authors: Keramati M,Aslani MM,Roohvand F

    更新日期:2020-02-01 00:00:00

  • Identification of Bordetella avium using the polymerase chain reaction.

    abstract::A DNA fragment from Bordetella pertussis, encoding the fim2 fimbrial subunit gene with adjacent sequences, was used as a probe for the detection of homologous sequences in chromosomal DNA of Bordetella avium. A 1.8 kb Sa1I-PstI fragment from the genome of B. avium, which hybridized with the probe, was isolated and seq...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1993.1071

    authors: Savelkoul PH,de Groot LE,Boersma C,Livey I,Duggleby CJ,van der Zeijst BA,Gaastra W

    更新日期:1993-09-01 00:00:00

  • Worldwide prevalence of emerging parasite Blastocystis in immunocompromised patients: A systematic review and meta-analysis.

    abstract:BACKGROUND:Blastocystis is one of the most common pathogens of the human intestine, caused by an emerging parasite, which can lead to severe symptoms and even death in immunocompromised patients. We aimed to determine the global prevalence of Blastocystosis infection in people with immunodeficiency. A systematic litera...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104615

    authors: Khorshidvand Z,Khazaei S,Amiri M,Taherkhani H,Mirzaei A

    更新日期:2020-11-24 00:00:00

  • Effect of sub-minimal inhibitory concentration ceftazidime on the pathogenicity of uropathogenic Escherichia coli.

    abstract::Uropathogenic Escherichia coli (UPEC) is the most prevalent causative agent of urinary tract infections (UTIs). The pathogenicity of UPEC relies on the expression of virulence factors which could be regulated by intercellular signal molecules. Our previous study found that sub-minimal inhibitory concentration ceftazid...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2021.104748

    authors: Feng W,Zhang L,Yuan Q,Wang Y,Yao P,Xia P,Sun F

    更新日期:2021-01-21 00:00:00

  • Preparation and evaluation of antibacterial potential of Pithecellobium dulce root extract against Gram positive and Gram negative bacteria.

    abstract::In the present study hexane, benzene, ethyl acetate and ethanol extracts of Pithecellobium dulce root were prepared using soxhlet extractor. The extracts were evaluated for antibacterial activity against one Gram positive (Staphylococcus aureus) and three Gram negative (Acetobacter aceti, Acetobacter aceti, Klebsiella...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.01.013

    authors: Bhat MA,Malik RA,Prakash P,Lone AM

    更新日期:2018-03-01 00:00:00

  • Differential expression of structural and functional proteins during bean common mosaic virus-host plant interaction.

    abstract::Bean common mosaic virus (BCMV), the most common seed-borne pathogen in Phaseolus vulgaris L. is known to cause severe loss in productivity across the globe. In the present study, proteomic analyses were performed for leaf samples from control (healthy) and susceptible BCMV infected plants. The differential expression...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2019.103812

    authors: Kumar S,Dhembla C,P H,Sundd M,Patel AK

    更新日期:2020-01-01 00:00:00

  • Characterization of outer membrane and secreted proteins of Leptospira interrogans serovar pomona.

    abstract::Outer membrane and secreted proteins were isolated from Leptospira interrogans serovar pomona and characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis, immunoblot and radioimmunoprecipitation techniques. The L. interrogans outer membranes were extracted with Triton X-114 and contained several pro...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(91)90014-2

    authors: Zuerner RL,Knudtson W,Bolin CA,Trueba G

    更新日期:1991-04-01 00:00:00

  • The effect of deletion of the edema factor on Bacillus anthracis pathogenicity in guinea pigs and rabbits.

    abstract::Bacillus anthracis secretes three major components, which assemble into two bipartite toxins: lethal toxin (LT), composed of lethal factor (LF) and protective antigen (PA) and edema toxin (ET), composed of edema factor (EF) and PA. EF is a potent calmodulin-dependent adenylate cyclase, which is internalized into the t...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2011.10.002

    authors: Levy H,Weiss S,Altboum Z,Schlomovitz J,Rothschild N,Glinert I,Sittner A,Kobiler D

    更新日期:2012-01-01 00:00:00

  • Hyperexpression of type III secretion system of Salmonella Typhi linked to a higher cytotoxic effect to monocyte-derived macrophages by activating inflammasome.

    abstract::Inflammasome activation is an important host response to infectious diseases, but the difference in inflammasome activation between typhoid fever and non-typhoidal Salmonella infection has been rarely studied. To determine whether inflammasome activation in macrophages after S. Typhi and S. Typhimurium infection is di...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104222

    authors: Lin HH,Chen HL,Janapatla RP,Chen CL,Chiu CH

    更新日期:2020-09-01 00:00:00

  • Restriction fragment length polymorphism of PCR amplified papE gene products is correlated with complete serotype among uropathogenic Escherichia coli isolates.

    abstract::Using whole bacteria, rather than extracted, purified DNA samples, we amplified the papE gene sequences from 63 Escherichia coli isolates belonging to O serogroups O1, O2 and O6. These isolates were from collections separated temporally as well as geographically: from four cities in the US and one in Sweden. PCR ampli...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1996.0033

    authors: Jantausch BA,Hull SI

    更新日期:1996-06-01 00:00:00