Abstract:
:Staphylococcus aureus (S. aureus) is one of several opportunistic microbial pathogens associated with many healthcare problems. In the present study, S. aureus was assessed for its biofilm-forming ability on materials routinely used in dental offices, including stainless steel (SS), polyethylene (PE), and polyvinyl chloride (PVC). Materials that were tested were characterized for roughness (Ra) and surface free energy (SFE). The adhesion forces exerted by S. aureus to each substratum were investigated using atomic force microscopy (AFM), and biofilm formation was quantitatively assessed by crystal violet staining assay. AFM measurements demonstrated that the strongest adhesion forces (20 nN) were exerted on the PE surfaces (P < 0.05) and depended more on Ra. In addition, the results of biofilm formation capability indicated that S. aureus exhibited more affinity to SS materials when compared to the other materials (P < 0.05). This ability of biofilm formation seems to be more correlated to SFE (R = 0.65). Hence, control of the surface properties of materials used in dental practices is of crucial importance for preventing biofilm formation on dental materials to be used for patients' dental care.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Merghni A,Bekir K,Kadmi Y,Dallel I,Janel S,Bovio S,Barois N,Lafont F,Mastouri Mdoi
10.1016/j.micpath.2016.12.014subject
Has Abstractpub_date
2017-02-01 00:00:00pages
129-134eissn
0882-4010issn
1096-1208pii
S0882-4010(16)30775-6journal_volume
103pub_type
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