当前位置： SCI文献检索 > MICROBIAL PATHOGENESIS期刊下所有文献 > Potential efficiency of conventional and advanced approaches used to detect Mycobacterium bovis in cattle.

Potential efficiency of conventional and advanced approaches used to detect Mycobacterium bovis in cattle.

Abstract：

:The present study was aimed to assess the prevalence and efficiency of techniques for the diagnosis of bovine tuberculosis (bTB) including enzyme-linked immunosorbent assay (ELISA), Gamma interferon assay (IFN-γ) and polymerase chain reaction (PCR) in comparison to skin tuberculin test and culture technique. A total of 2600 cross-breed dairy cattle in Menoufia and Daqahlia governorates were tested by the single intradermal tuberculin test where the disease prevalence was 1.8%. Serum and whole blood samples were collected from positive tuberculin reactors for ELISA and IFN-γ assay, respectively. After slaughtering of positive tuberculin reactors, the post-mortem examination was carried out and tissue samples were collected for the bacteriological examination and PCR. The percentage of visible lesions of tuberculin reactors was 78.7%, while non-visible lesions were 21.27%. Culture technique revealed that the percentage of bTB was 63.8%. The ELISA and IFN-γ assay using short-term culture filtrate (ST-CF) prepared antigen revealed higher sensitivity (72.3% and 82.9%) than the bovine purified protein derivative (PPD-B) antigen. Although prepared ST-CF antigen has great efficiency and eligibility for the diagnosis of bTB, PCR appeared to have a higher sensitivity (85.1%) than other diagnostic methods when dealing with post-mortem samples. Gamma interferon assay using ST-CF antigen is recommended for antemortem diagnosis of bTB in cattle.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Algammal AM,Wahdan A,Elhaig MM

doi

10.1016/j.micpath.2019.103574

subject

Has Abstract

pub_date

2019-09-01 00:00:00

pages

103574

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(19)30583-2

journal_volume

134

pub_type

杂志文章

相关文献

MICROBIAL PATHOGENESIS文献大全
  • Detection of antibodies against Aggregatibacter actinomycetemcomitans in serum and saliva through ELISA in periodontally healthy individuals and individuals with chronic periodontitis.

    abstract：BACKGROUND:Periodontitis is a persistent polymicrobial infection, which leads to chronic inflammation in the tooth supporting tissues. Aggregatibacter actinomycetemcomitans are normal commensals of oral cavity but are low in number in periodontally healthy subjects. They are one of the major pathogens aetiologically li...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.10.007

    authors： Gadekar NB,Hosmani JV,Bhat KG,Kotrashetti VS,Nayak RS,Babji DV,Pattanshetty SM,Joshi VM,Bansode RA

    更新日期：2018-12-01 00:00:00

  • Human endogenous retrovirus env genes: Potential blood biomarkers in lung cancer.

    abstract：:Lung cancer, the leading cause of cancer mortality, needs urgent development of newly qualified diagnostic and therapeutic biomarkers. Recently, Human Endogenous Retroviruses (HERVs) have been introduced for cancer diagnosis. In this case-control study, we have collected blood samples from 60 lung cancer patients and ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.12.040

    authors： Zare M,Mostafaei S,Ahmadi A,Azimzadeh Jamalkandi S,Abedini A,Esfahani-Monfared Z,Dorostkar R,Saadati M

    更新日期：2018-02-01 00:00:00

  • The genus Aeromonas: A general approach.

    abstract：:The genus Aeromonas comprises more than thirty Gram-negative bacterial species which mostly act as opportunistic microorganisms. These bacteria are distributed naturally in diverse aquatic ecosystems, where they are easily isolated from animals such as fish and crustaceans. A capacity for adaptation also makes Aeromon...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,评审

    doi：10.1016/j.micpath.2019.02.036

    authors： Gonçalves Pessoa RB,de Oliveira WF,Marques DSC,Dos Santos Correia MT,de Carvalho EVMM,Coelho LCBB

    更新日期：2019-05-01 00:00:00

  • Antibacterial studies of hydroxyspiro[indoline-3,9-xanthene]trione against spiro[indoline3,9-xanthene]trione and their use as acetyl and butyrylcholinesterase inhibitors.

    abstract：:Xanthene derivatives are well known for their effective biological activities. In search of effective antibacterial agents, the spiro[indoline3,9-xanthene]-trione (A) and hydroxy-spiro[indoline-3,9-xanthene]-trione (B), were synthesized and tested for in vitro antibacterial activity against Staphylococcus aureus and E...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.03.002

    authors： Barmak A,Niknam K,Mohebbi G,Pournabi H

    更新日期：2019-05-01 00:00:00

  • Chlamydia pneumoniae multiplies in human endothelial cells in vitro.

    abstract：:The ability of three C. pneumoniae isolates, Kajaani 6, Helsinki 12 and TW-183, to grow in human umbilical vein endothelial cells (HUVEC) and in an immortalized endothelial cell line EA.hy 926 was studied. All C. pneumoniae isolates were capable of multiplying in endothelial cells. EA.hy926 cells could support the gro...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1994.1032

    authors： Kaukoranta-Tolvanen SS,Laitinen K,Saikku P,Leinonen M

    更新日期：1994-04-01 00:00:00

  • Phylogenetic analysis and molecular characteristics of 17 porcine reproductive and respiratory syndrome virus isolates in Southern China from 2010 to 2011.

    abstract：:We analyzed the complete genomic sequences of 17 porcine reproductive and respiratory syndrome virus (PRRSV) isolates from Southern China obtained between 2010 and 2011 and found that four of seven isolates from 2011 were closely related to the JXA1-R strain (vaccine virus of JXA1). This close relationship between fie...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2013.09.005

    authors： Zhang M,Xie J,Sun L,Cao Z,Gu H,Deng S,Chen Y,Cao Z,Tang F,Su S,Zhang G

    更新日期：2013-12-01 00:00:00

  • Ultrastructure and phylogenetic characterization of the microsporidian parasite Heterosporis lessepsianus n. sp. (Microsporidia: Glugeidae) infecting the lizardfish Saurida lessepsianus (Pisces: Synodontidae) inhabiting the Red Sea.

    abstract：:Heterosporosis is an increasingly important microsporidian disease distributed worldwide, impacting wild and farmed-raised fish in both fresh and marine water environments. Twenty three out of 130 (17.69%) of the lizardfish Saurida lessepsianus were found to be naturally infected with microsporidian parasites. The rat...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.02.025

    authors： Al Quraishy S,Abdel-Gaber R,El Deeb N,Maher S,Al-Shaebi E,Abdel-Ghaffar F

    更新日期：2019-05-01 00:00:00

  • Use of confocal microscopy to detect Salmonella typhimurium within host cells associated with Spv-mediated intracellular proliferation.

    abstract：:The major limitation in histological examination of orally inoculated mice of Salmonella typhimurium has been the difficulty of attaining high enough levels for immunochemical detection. This problem has been solved by the use of confocal laser scanning microscopy (CLSM) analysis, which allows detection of bacteria in...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.2000.0370

    authors： Matsui H,Eguchi M,Kikuchi Y

    更新日期：2000-07-01 00:00:00

  • Nanoparticles and their antimicrobial properties against pathogens including bacteria, fungi, parasites and viruses.

    abstract：:In recent year, propagation and resistance of pathogenic microorganisms (bacteria, fungi and virals) to common antimicrobial agents has led to serious health and food problems. Today, nanotechnology science and nanoparticles (NPs) have been identified as a new approach to deal with this problem because of their inhere...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,评审

    doi：10.1016/j.micpath.2018.08.008

    authors： Khezerlou A,Alizadeh-Sani M,Azizi-Lalabadi M,Ehsani A

    更新日期：2018-10-01 00:00:00

  • Alternative method in Galleria mellonella larvae to study biofilm infection and treatment.

    abstract：:In vivo studies are crucial decision-maker step in order to translate in vitro data to an applied therapy. Considering this we describe a simple method that analyzes and quantifies biofilm formation inside the Galleria mellonella larvae. Toothbrush bristles were employed as an abiotic surface to mimic a medical device...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.103756

    authors： Campos-Silva R,Brust FR,Trentin DS,Macedo AJ

    更新日期：2019-12-01 00:00:00

  • In vitro studies of epithelium-associated crystallization caused by uropathogens during urinary calculi development.

    abstract：:Infectious urinary stones account for about 10% of all urinary stones. In 50% of cases urolithiasis is a recurrent illness, which can lead to the loss of a kidney if not properly treated. One of the reasons for recurrence of the disease may be the ability of bacteria to invade urothelial cells, persist in the host cel...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2014.04.007

    authors： Torzewska A,Budzyńska A,Białczak-Kokot M,Różalski A

    更新日期：2014-06-01 00:00:00

  • Vancomycin-induced biofilm formation by methicillin-resistant Staphylococcus aureus is associated with the secretion of membrane vesicles.

    abstract：:Chronic burn wound infections caused by Stapyhylococcus aureus (S. aureus) are largely associated with biofilm formation. However, the mechanism by which S. aureus form biofilm in clinical environments is far less understood. In the present study we addressed the association between biofilm formation and membrane vesi...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.07.004

    authors： He X,Yuan F,Lu F,Yin Y,Cao J

    更新日期：2017-09-01 00:00:00

  • Different frequencies of memory B-cells induced by tetanus, botulinum, and heat-labile toxin binding domains.

    abstract：:Along with robust immunogenicity, an ideal vaccine candidate should be able to produce a long lasting protection. In this regard, the frequency of memory B-cells is possibly an important factor in memory B-cell persistency and duration of immunological memory. On this basis, binding domains of tetanus toxin (HcT), bot...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.12.003

    authors： Rezaie E,Nekoie H,Miri A,Oulad G,Ahmadi A,Saadati M,Bozorgmehr M,Ebrahimi M,Salimian J

    更新日期：2019-02-01 00:00:00

  • Isolates of the Enterobacter cloacae complex induce apoptosis of human intestinal epithelial cells.

    abstract：:Strains of the Enterobacter cloacae complex are becoming increasingly important human pathogen. The aim of the study was to identify, by sequencing the hsp60 gene, the species of clinical isolates phenotypically identified as E. cloacae and to examine them for virulence-associated properties: the ability of adhesion, ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2010.04.003

    authors： Krzymińska S,Koczura R,Mokracka J,Puton T,Kaznowski A

    更新日期：2010-09-01 00:00:00

  • The regulatory role of C1q on Helicobacter pylori-induced inflammatory cytokines secretion in THP-1 cells.

    abstract：:C1q, as a LAIR-1 ligand, maintains monocytes quiescence and possess immunosuppressive properties. To understand the roles and molecular mechanisms, C1q mediated inflammation cytokines and several pivotal proteins in THP-1 cells after H. pylori infection were detected. The results showed that the expression of IL-8, IL...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.04.017

    authors： Zhang Y,Li J,Rong Q,Xu Z,Ding Y,Cao Q,Ji X,Zhao H,Wu Y,Li B

    更新日期：2019-06-01 00:00:00

  • Isolation and characterization of a broad-spectrum phage of multiple drug resistant Salmonella and its therapeutic utility in mice.

    abstract：:Salmonella are causes of livestock, poultry, and other animal diseases but they also have the potential to infect people. Currently, antibiotics are the first choice for treatment of Salmonella infections. Thus, the utility of phage has become the research focus for scientists for several reasons. There are efficient,...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.10.042

    authors： Tang F,Zhang P,Zhang Q,Xue F,Ren J,Sun J,Qu Z,Zhuge X,Li D,Wang J,Jiang M,Dai J

    更新日期：2019-01-01 00:00:00

  • Prevalence of active tuberculosis infection in transplant recipients: A systematic review and meta-analysis.

    abstract：INTRODUCTION:Tuberculosis (TB) is considered as a serious complication of organ transplant; therefore, the detection and appropriate treatment of active TB infection is highly recommended for the reduction of mortality in the future. The aim of this review was to conduct a systematic review and meta-analysis assessing ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.micpath.2019.103894

    authors： Mamishi S,Pourakbari B,Moradzadeh M,van Leeuwen WB,Mahmoudi S

    更新日期：2020-02-01 00:00:00

  • Antibacterial substances produced by pathogen inhibitory gut bacteria in Labeo rohita: Physico-chemical characterization, purification and identification through MALDI-TOF mass spectrometry.

    abstract：:Application of antibiotics to combat bacterial diseases in fish has been criticized due to likely emergence of drug resistance. Therefore, investigation of new bioactive compounds from natural sources has been taken into account. This study was designed to purify and characterize the bioactive compound in the cell fre...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.02.028

    authors： Mukherjee A,Banerjee G,Mukherjee P,Ray AK,Chandra G,Ghosh K

    更新日期：2019-05-01 00:00:00

  • Reduced virulence of a hfq mutant of Pseudomonas aeruginosa O1.

    abstract：:The Sm-like protein Hfq has been implicated in the regulation of sigmaS-dependent and sigmaS-independent genes in E. coli and in the regulation of virulence factors in both, Yersinia enterocolitica and Brucella abortus. Here, we have studied the effect of Hfq on virulence and stress response of Pseudomonas aeruginosa ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/s0882-4010(03)00149-9

    authors： Sonnleitner E,Hagens S,Rosenau F,Wilhelm S,Habel A,Jäger KE,Bläsi U

    更新日期：2003-11-01 00:00:00

  • A new subtype of a natural viral inhibitor (CVI) that is stable in the gastrointestinal tract.

    abstract：:A virus inhibitor found in gastric secretions and in extracts from gastrointestinal tissues is described. The inhibitor shares a number of characteristics with the recently described contact-blocking virus inhibitor (CVI), which is produced by unstimulated cells in culture, and occurs naturally in some body fluids. Th...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(86)90048-3

    authors： Baron JL,Li JL,McKerlie ML,Shabot JM,Coppenhaver DH

    更新日期：1986-06-01 00:00:00

  • Antimicrobial activity of crude fractions and morel compounds from wild edible mushrooms of North western Himalaya.

    abstract：:The antimicrobial properties of morel compounds from wild edible mushrooms (Morchella esculenta and Verpa bohemica) from Kashmir valley was investigated against different clinical pathogens. The butanol crude fraction of most popular or true morel M. esculenta showed highest 19 mm IZD against E.coli while as same frac...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.03.005

    authors： Shameem N,Kamili AN,Ahmad M,Masoodi FA,Parray JA

    更新日期：2017-04-01 00:00:00

  • Systems biology studies in Pseudomonas aeruginosa PA01 to understand their role in biofilm formation and multidrug efflux pumps.

    abstract：:The antimicrobial resistance (AMR) exhibited against broad spectrum and new generation antibiotics used for Pseudomonas infections is a major threat and renders the treatment ineffective. In our present study, we have used a computational approach to understand various drug resistance mechanisms which contribute to Mu...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.103668

    authors： Miryala SK,Anbarasu A,Ramaiah S

    更新日期：2019-11-01 00:00:00

  • Apoptosis induced by bacterial pathogens.

    abstract：:Programmed cell death is part of normal development and homeostasis. Apoptosis induced by bacteria appears to contribute to infectious diseases. Some bacteria produce toxins to kill host cells by the same pathway, apoptosis, through different mechanisms including pore formation, protein synthesis inhibition or adenyla...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,评审

    doi：10.1006/mpat.1994.1066

    authors： Chen Y,Zychlinsky A

    更新日期：1994-10-01 00:00:00

  • alpha-Hydroxynitrile lyase protein from Xylella fastidiosa: Cloning, expression, and characterization.

    abstract：:Xylella fastidiosa is a xylem-restricted plant pathogen that causes a range of diseases in several and important crops. Through comparative genomic sequence analysis many genes were identified and, among them, several potentially involved in plant-pathogen interaction. The experimental determination of the primary seq...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2009.06.007

    authors： Caruso CS,de Fátima Travensolo R,de Campus Bicudo R,de Macedo Lemos EG,Ulian de Araújo AP,Carrilho E

    更新日期：2009-09-01 00:00:00

  • Inhibition of TLR8 mediated signaling promotes BCG induced apoptosis in THP-1 cells.

    abstract：:Apoptosis was considered as one of the important host defense mechanisms against mycobacteria infection. In macrophage, the main target cell of Mycobacterium tuberculosis, apoptosis after infection could help kill the bacillus inside and process the antigens for further presentation and proper immune response. Here, w...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2015.11.028

    authors： Tang J,Zhan L,Qin C

    更新日期：2016-04-01 00:00:00

  • Dysbiosis of gut microbiota in patients with esophageal cancer.

    abstract：:A number of studies have identified that gut microbiota influences the development of cancer. However, there is little known about gut microbiota and esophageal cancer (EC). The aim of this study was to investigate the gut microbiota profile associated with EC. In this study, 23 patients with EC and 23 sex- and age-ma...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104709

    authors： Deng Y,Tang D,Hou P,Shen W,Li H,Wang T,Liu R

    更新日期：2020-12-27 00:00:00

  • Probiotic Lactobacillus fermentum KU200060 isolated from watery kimchi and its application in probiotic yogurt for oral health.

    abstract：:Lactobacillus fermentum KU200060 was isolated from watery kimchi and its probiotic characteristics were evaluated, including tolerance to artificial gastric acid and bile salt, production of enzymes, ability to adhere to HT-29 cells, and antibiotic susceptibility. The antibacterial and antibiofilm effects of L. fermen...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104430

    authors： Lim SM,Lee NK,Kim KT,Paik HD

    更新日期：2020-10-01 00:00:00

  • Treatment with 3'-deoxyadenosine and deoxycoformycin in mice infected by Trypanosoma cruzi and its side effect on purinergic enzymes.

    abstract：:The aim of this study was to evaluate the efficacy of 3'-deoxyadenosine and deoxycoformycin combination in the treatment of mice infected by T. cruzi, as well as to verify the influence of the treatment on purinergic enzymes. Heart and serum samples were collected from 60 mice (30 infected and 30 uninfected) at day 12...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.10.030

    authors： do Carmo GM,Doleski PH,de Sá MF,Grando TH,Azevedo MI,Manzoni AG,Leal DBR,Gressler LT,Henker LC,Mendes RE,Baldissera MD,Monteiro SG,Stefani LM,Da Silva AS

    更新日期：2017-12-01 00:00:00

  • Production and characterization of monoclonal antibodies against GII.6 norovirus virus-like particles.

    abstract：:In this study we generated and characterized a series of monoclonal antibodies (mAbs) against GII.6 norovirus (NoV) virus like particles (VLPs). Mice were immunized with purified GII.6 NoV VLPs and peptide-bovine serum albumin (BSA) conjugates with the peptide sequence (31 aa) derived from the trypsin cleavage region....

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104100

    authors： Qiu S,Zheng L,Qin C,Yin X,Ma J,Liu J,Yang Z,Li C,Wang Y,Wang M,Qi Y,Huo Y

    更新日期：2020-02-26 00:00:00

  • The roles of hormones in the modulation of growth and virulence genes' expressions in UPEC strains.

    abstract：BACKGROUND:Host factors such as hormones are known to modulate growth, virulence and antibiotic susceptibility of bacteria. In the present study, the effect of norepinephrine (NE) and estradiol (Est) on growth and expression levels of virulence genes (usp, sfa/foc, cnf1, aer) of uropathogenic E. coli (UPEC) strains C7 ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.05.019

    authors： Gümüş D,Kalaycı Yüksek F,Sefer Ö,Yörük E,Uz G,Anğ Küçüker M

    更新日期：2019-07-01 00:00:00