Use of confocal microscopy to detect Salmonella typhimurium within host cells associated with Spv-mediated intracellular proliferation.

Abstract:

:The major limitation in histological examination of orally inoculated mice of Salmonella typhimurium has been the difficulty of attaining high enough levels for immunochemical detection. This problem has been solved by the use of confocal laser scanning microscopy (CLSM) analysis, which allows detection of bacteria in the immunostained sections of mouse spleens at a minimum rate of approximately 1000 colony-forming units (cfu)/spleen. Here, we demonstrate that over 80% of salmonellae of the wild type of S. typhimurium were detected intracellularly within Mac-1 positive cells by the CLSM analysis of immunostained sections from spleens in orally or subcutaneously inoculated mice. Only 40% of salmonellae of the spv -deleted strain were detected inside Mac-1 positive cells. These data suggest that the spv genes play a key role in intracellular proliferation within phagocytes in the mouse spleen.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Matsui H,Eguchi M,Kikuchi Y

doi

10.1006/mpat.2000.0370

subject

Has Abstract

pub_date

2000-07-01 00:00:00

pages

53-9

issue

1

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(00)90370-X

journal_volume

29

pub_type

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