Abstract:
:The major limitation in histological examination of orally inoculated mice of Salmonella typhimurium has been the difficulty of attaining high enough levels for immunochemical detection. This problem has been solved by the use of confocal laser scanning microscopy (CLSM) analysis, which allows detection of bacteria in the immunostained sections of mouse spleens at a minimum rate of approximately 1000 colony-forming units (cfu)/spleen. Here, we demonstrate that over 80% of salmonellae of the wild type of S. typhimurium were detected intracellularly within Mac-1 positive cells by the CLSM analysis of immunostained sections from spleens in orally or subcutaneously inoculated mice. Only 40% of salmonellae of the spv -deleted strain were detected inside Mac-1 positive cells. These data suggest that the spv genes play a key role in intracellular proliferation within phagocytes in the mouse spleen.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Matsui H,Eguchi M,Kikuchi Ydoi
10.1006/mpat.2000.0370subject
Has Abstractpub_date
2000-07-01 00:00:00pages
53-9issue
1eissn
0882-4010issn
1096-1208pii
S0882-4010(00)90370-Xjournal_volume
29pub_type
杂志文章abstract::Besides the well characterized role in glycolysis, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been implicated in virulence of pathogenic micro-organisms and because of its cell surface location, it has been shown to act as an adhesin for colonization of tissue surfaces both for pathogenic and non-pathogenic ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2010.11.008
更新日期:2011-06-01 00:00:00
abstract::Staphylococcus aureus (S. aureus) is one of several opportunistic microbial pathogens associated with many healthcare problems. In the present study, S. aureus was assessed for its biofilm-forming ability on materials routinely used in dental offices, including stainless steel (SS), polyethylene (PE), and polyvinyl ch...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2016.12.014
更新日期:2017-02-01 00:00:00
abstract::Mannheimia(Pasteurella)haemolytica serotype 1 is the primary causative agent responsible for bovine pneumonic mannheimiosis, also known as shipping fever in cattle. The bacterium produces a variety of virulence factors, foremost of which is the exotoxic leukotoxin. The leukotoxin is a calcium-dependent cytolysin that ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/s0882-4010(03)00033-0
更新日期:2003-05-01 00:00:00
abstract::The non-fimbrial adhesin NFA-4 from uropathogenic Escherichia coli O7:K98:H6 mediates the agglutination of human red cells (RBC), notably of blood group MM. The adhesin can be separated from the bacteria by heat extraction and was purified to homogeneity by ammonium sulphate precipitation and anion exchange chromatogr...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/0882-4010(89)90077-6
更新日期:1989-05-01 00:00:00
abstract::The role of Porphyromonas gingivalis cysteine proteinases (gingipains) in the evasion of host cell-mediated immunity has not been fully determined. In this study, modulation by gingipains of accessory and co-stimulatory molecule expression on human CD4(+) T cells was evaluated. Arg-gingipain rather than Lys-gingipain ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2005.01.001
更新日期:2005-02-01 00:00:00
abstract::Principle component analysis (PCA) was employed to examine the effect of the exerted treatments on the beef shelf life as well as discovering the correlations between the studied responses. Considering the variability of the dimensions of the responses, correlation coefficients were applied to form the matrix and extr...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2017.02.013
更新日期:2017-04-01 00:00:00
abstract::During the past decade, cationic antimicrobial peptides (CAPs) have gained particular interest among researchers, since they often display broad-spectrum antimicrobial activity and low possibility of resistance emergence. This study aimed to investigate in vitro effectiveness of Mastoparan-1 (MP-1), a tetradecapeptide...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.04.008
更新日期:2018-06-01 00:00:00
abstract::Chronic hepatitis B (CHB) infection is a typical inflammatory disease characterized by a dysregulated expression of cytokines, which contributes to the pathogenesis of chronic Hepatitis B virus (HBV) infection. IL-36 cytokines (IL-36α, IL-36β, IL-36γ and IL-36Ra) are important players in infection and immunity. Howeve...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2017.11.023
更新日期:2017-12-01 00:00:00
abstract::Our previous studies have shown that the adhesive ability of Enterococcus faecalis is dependent on the strain and is further modified by growth in serum. The data reported here demonstrate that E. faecalis adherence is mediated by carbohydrate residues present on the bacterial cell surface. Some of these (D-mannose an...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/0882-4010(91)90036-a
更新日期:1991-12-01 00:00:00
abstract::Because of the close genetic relatedness between apes and humans, apes are susceptible to many human infectious agents and can serve as carriers of these pathogens. Consequently, they present a serious health hazard to humans. Moreover, many emerging infectious diseases originate in wildlife and continue to threaten h...
journal_title:Microbial pathogenesis
pub_type: 杂志文章,评审
doi:10.1016/j.micpath.2014.09.003
更新日期:2014-12-01 00:00:00
abstract::Nonencapsulated Haemophilus influenzae frequently persists in the lungs of chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF) patients for prolonged periods of time. The bacteria are not eradicated by antibiotic treatment of the patients or by specific antibodies that are found in the sputum and ser...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1998.0269
更新日期:1999-05-01 00:00:00
abstract::Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are two major omega-3 polyunsaturated fatty acids (n-3 PUFAs) with antimicrobial properties. In this study, we evaluated the potential antibacterial and antibiofilm activities of DHA and EPA against two periodontal pathogens, Porphyromonas gingivalis (P. gingi...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2016.08.025
更新日期:2016-10-01 00:00:00
abstract::Peste des petits ruminants (PPR), an economically important viral transboundary disease of small ruminants is not only prevalent in Pakistan but also in other countries where people rely on agriculture and animal products. The present study was aimed at describing the pathology and antigen localization in natural PPR ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.02.009
更新日期:2018-04-01 00:00:00
abstract::Saffron (Crocus sativus L.) is an important plant in medicine. The Kashmir Valley (J&K, India) is one of the world's largest and finest saffron producing regions. However, over the past decade, there has been a strong declining trend in saffron production in this area. Plant Growth Promoting Rhizobacteria (PGPR) are f...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2021.104734
更新日期:2021-01-08 00:00:00
abstract:CONTEXT:Hepatitis E virus (HEV) as a hepatotropic virus is one of the major global health concerns. Autochthonous HEV transmitted by oral fecal-route in poor sanitation conditions as well as vertical and rarely blood transfusion. HEV occurrence is more common in developing countries and recently increased in developed ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章,评审
doi:10.1016/j.micpath.2017.06.045
更新日期:2017-09-01 00:00:00
abstract::Adhesion is the first step for Candida species to form biofilms on medical devices implanted in the human host. Both the physicochemical nature of the biomaterial and cell wall proteins (CWP) of the pathogen play a determinant role in the process. While it is true that some CWP have been identified in vitro, little is...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2017.04.009
更新日期:2017-06-01 00:00:00
abstract::Toxoplasmosis is a major public health concern due to neurotropic nature and role in the development of mental and behavioral disorders. Alzheimer's disease (AD) is an important nervous disease that results in the reduction of the amount of beta-amyloid plaque deposition and irreversible loss of neurons in the brain. ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章,meta分析
doi:10.1016/j.micpath.2019.103751
更新日期:2019-12-01 00:00:00
abstract::The effects of depleting CD4+ and CD8+ T cells on macrophage recruitment have been analyzed for bronchoalveolar lavage (BAL) populations from mice with primary or secondary influenza pneumonia. Macrophages were characterized by both the capacity to engulf latex particles and the expression of mRNA for a 65 kD heat sho...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1993.1008
更新日期:1993-01-01 00:00:00
abstract::Three bacterial isolates were identified from infected potato tubers showing soft and blackleg like symptoms as well as one isolate from infected pear tree showing crown gall symptom. Conventional and molecular identification proved that bacterial isolates belonging to Pectobacterium carotovorum subsp. carotovorum, Pe...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.05.032
更新日期:2018-08-01 00:00:00
abstract::Vibrio cholerae elaborates zonula occludens toxin (Zot), a protein that increases the permeability of small intestinal mucosa by opening intercellular tight junctions. The zot gene is located, together with the genes encoding CT and Ace enterotoxins, within the genome of V. cholerae filamentous phage CTXsmall ef, Cyri...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1999.0312
更新日期:1999-12-01 00:00:00
abstract::The extracellular hemolysin (AHH1) gene of Aeromonas hydrophila ATCC7966 was cloned into Charomid9-28 in Escherichia coli DH1, and its complete nucleotide sequence determined. Escherichia coli carrying this gene expressed an extracellular heat-labile hemolysin for rabbit red blood cells. The minimum size of the coding...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/0882-4010(91)90049-g
更新日期:1991-09-01 00:00:00
abstract:BACKGROUND:Brucella is a Gram-negative and facultative intracellular organism that causes brucellosis, a common zoonotic disease. Over 500,000 people are annually affected by brucellosis. Brucella is highly infectious through inhalation route; for this reason it is used for biological warfare aims. This study aimed to ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.04.050
更新日期:2018-08-01 00:00:00
abstract::HPV persistent infection is a main event leading to the development of cervical intraepithelial neoplasia and cervical cancer. Earlier to distinguish HPV persistent and transient infection is meaningful but the methods are limited. This study used 16S rDNA sequencing to determine the cervicovaginal microbiota of HPV p...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104617
更新日期:2020-11-15 00:00:00
abstract::Due to the growing number of multi-resistant Candida spp., adjuvant treatments that may help combat these fungal pathogens are relevant and useful. This study evaluated the immunomodulation and anti-Candida activity of Lactobacillus rhamnosus (LR), Lactobacillus acidophilus and Lactobacillus paracasei suspensions, eit...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2017.08.006
更新日期:2017-09-01 00:00:00
abstract::We analyzed the complete genomic sequences of 17 porcine reproductive and respiratory syndrome virus (PRRSV) isolates from Southern China obtained between 2010 and 2011 and found that four of seven isolates from 2011 were closely related to the JXA1-R strain (vaccine virus of JXA1). This close relationship between fie...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2013.09.005
更新日期:2013-12-01 00:00:00
abstract::Staphylococcus aureus (S.aureus), a Gram-positive organism, is a frequent cause of subclinical mastitis. Geniposide, an iridoid glucoside isolated from the fruits of Gardenia jasminoides, has been reported to exhibit antibacterial and anti-inflammatory properties. The ability of S. aureus internalizing into bovine mam...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.10.002
更新日期:2018-12-01 00:00:00
abstract::Chlamydia persistence is a viable but non-replicative stage, induced by several sub-lethal stressor agents, including beta-lactam antibiotics. So far, no data about the connection between doxycycline and chlamydial persistence has been described in literature. We investigated the ability of doxycycline to induce C. tr...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2020.104347
更新日期:2020-10-01 00:00:00
abstract::Salmonella is an important cause of food-borne infection worldwide. Detection of outbreaks caused by Salmonella spp. relies on suitable and robust methods for genotyping. Little is known about the genetic diversity of the Salmonella enterica subspecies enterica serotype Infantis strains isolated from human sources in ...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2016.10.012
更新日期:2016-11-01 00:00:00
abstract::Childhood pneumonia has been considered as a major cause of child morbidity and mortality worldwide. We aimed to investigate the effect of resveratral in synergizing with oral amoxicillin to improve the treatment outcome of oral amoxicillin administration against childhood fast breathing pneumonia. 647 children diagno...
journal_title:Microbial pathogenesis
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.micpath.2017.11.062
更新日期:2018-01-01 00:00:00
abstract::We have developed a system whereby heterologous DNA encoding an antigen from an enteropathogen may be recombined into the chromosome of an attenuated Salmonella carrier strain. The system involves two steps: (i) integration of a hisOG deletion mutation into the chromosome; (ii) replacement of the hisOG deletion by the...
journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/0882-4010(88)90002-2
更新日期:1988-12-01 00:00:00