Abstract:
:We have developed a system whereby heterologous DNA encoding an antigen from an enteropathogen may be recombined into the chromosome of an attenuated Salmonella carrier strain. The system involves two steps: (i) integration of a hisOG deletion mutation into the chromosome; (ii) replacement of the hisOG deletion by the complete hisOG region and the segment of heterologous DNA which encodes the antigen of interest. Recombinants may be selected (his+). The system was used to integrate the genes encoding K88 fimbriae from enterotoxigenic Escherichia coli into the chromosome of a galE mutant of Salmonella typhimurium (LT2H1). Recombinants were detected at a frequency of between 1.0 x 10(-3) and 1.5 x 10(-3). A variety of tests confirmed that the K88 genes were integrated into the chromosome of LT2H1 and were expressed. The stability of the recombinant was tested both in vivo and in vitro. When administered orally to mice, the recombinant elicited a serum antibody response to K88, and retained the Salmonella vaccine potential of the vector strain.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Hone D,Attridge S,van den Bosch L,Hackett Jdoi
10.1016/0882-4010(88)90002-2subject
Has Abstractpub_date
1988-12-01 00:00:00pages
407-18issue
6eissn
0882-4010issn
1096-1208journal_volume
5pub_type
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
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更新日期:2000-05-01 00:00:00
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
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更新日期:1992-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.2000.0384
更新日期:2000-10-01 00:00:00
abstract::The involvement of nitric oxide (NO) in host defense and cytoprotective functions in murine salmonellosis has been reported. Salmonella mutants with the altered sigma factor RpoS (sigmaS) are less virulent and are susceptible to various stresses. This study investigated the role of the rpoS gene of Salmonella enterica...
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.04.041
更新日期:2019-07-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1016/j.micpath.2015.09.012
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2012.02.001
更新日期:2012-05-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2017.05.021
更新日期:2017-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0882-4010(90)90080-a
更新日期:1990-02-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.02.007
更新日期:2019-06-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2019.04.025
更新日期:2019-06-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1998.0211
更新日期:1998-09-01 00:00:00