Abstract:
:The aim of this study was to evaluate the efficacy of 3'-deoxyadenosine and deoxycoformycin combination in the treatment of mice infected by T. cruzi, as well as to verify the influence of the treatment on purinergic enzymes. Heart and serum samples were collected from 60 mice (30 infected and 30 uninfected) at day 12 post-infection. To verify treatment efficacy, parasitemia was monitored, and the treatment with 3'-deoxy adenosine and deoxycoformycin combination was able to reduce it, but had no curative effect on mice. Seric activities of NTPDase (ATP and ADP substrate) and ADA were increased significantly in untreated mice infected by T. cruzi compared to the negative control, as well as mice treated with 3'-deoxyadenosine and deoxycoformycin (alone or combined) modulated the activity of NTPDase (ATP and ADP substrate), preventing them from increasing in infected animals (activity similar to healthy animals). Treatment with deoxycoformycin alone and associated with 3'-deoxyadenosine modulated the activity of ADA preventing them from increasing in infected animals. However, seric activities of ADA in mice treated with 3'-deoxyadenosine (cordycepin) alone does not modify the ADA activity compared with infected and non-treated mice. However, the 5'-nucleotidase activity decreased significantly in infected untreated animals and the same occurred in infected and treated animals with deoxycoformycin and 3'-deoxyadenosine. However, treatment with deoxycoformycin associated with 3'-deoxyadenosine preventing them from decreasing the 5'-nucleotidase activity. Therefore, we conclude that the treatments did not have curative success for mice infected by T. cruzi. However, the treatments were able to modulate the purinergic enzymes during the infection by T. cruzi, which may contribute to reduce the inflammatory damage in heart.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
do Carmo GM,Doleski PH,de Sá MF,Grando TH,Azevedo MI,Manzoni AG,Leal DBR,Gressler LT,Henker LC,Mendes RE,Baldissera MD,Monteiro SG,Stefani LM,Da Silva ASdoi
10.1016/j.micpath.2017.10.030subject
Has Abstractpub_date
2017-12-01 00:00:00pages
51-56eissn
0882-4010issn
1096-1208pii
S0882-4010(17)30824-0journal_volume
113pub_type
杂志文章abstract::Endometritis is commonly occurred in dairy cows after calving and results in a great deal of property damage. Although numerous studies have been performed to find the therapeutic agents for endometritis, the incidence of this disease remains high. Short-chain fatty acids (SCFAs), the major metabolic products of anaer...
journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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pub_type: 杂志文章
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
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更新日期:1994-05-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2008.04.008
更新日期:2008-08-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2005.11.003
更新日期:2006-02-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章,评审
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journal_title:Microbial pathogenesis
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pub_type: 杂志文章
doi:10.1016/j.micpath.2006.07.006
更新日期:2006-12-01 00:00:00
abstract::Pyelonephritis induces an inflammatory process in the renal parenchyma, which may occur as a result of excessive reactive nitrogen intermediates (RNI) and reactive oxygen species (ROS) and/or impaired antioxidant capacity. In the present investigation, contribution of free radicals to the development of acute pyelonep...
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doi:10.1016/j.micpath.2008.08.003
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doi:10.1016/j.micpath.2018.07.021
更新日期:2018-10-01 00:00:00
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2015.10.002
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2018.08.053
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abstract::In the present study, we isolated Lactobacillus sakei strain DGH5 from raw beef meat. This bacterium plays an inhibitory effect against food-spoiling bacteria and food-borne pathogens, including Listeria monocytogenes, a gram-positive and pathogenic bacterium. Lactobacillus sakei strain DGH5 was identified through bot...
journal_title:Microbial pathogenesis
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更新日期:2017-05-01 00:00:00