Abstract:
:Pyelonephritis induces an inflammatory process in the renal parenchyma, which may occur as a result of excessive reactive nitrogen intermediates (RNI) and reactive oxygen species (ROS) and/or impaired antioxidant capacity. In the present investigation, contribution of free radicals to the development of acute pyelonephritis induced by planktonic and biofilm cells of Pseudomonas aeruginosa was studied. Increase in production of RNI and ROS in urine, bladder and renal tissue following infection with P. aeruginosa was observed which correlated with bacterial load, neutrophil recruitment and malondialdehyde (MDA). Evaluation of the data revealed that excessive production of free radicals causes tissue damage leading to bacterial persistence in host's tissues. Treatment of mice with N-acetylcysteine (NAC), a potent antioxidant, lead to significant amelioration of oxidative stress and subsequent decrease in bacterial titer, neutrophil influx, MDA as well as tissue pathology highlighting important role of free radicals in P. aeruginosa induced pyelonephritis. Results of the present study bring out that production of RNI and ROS contributes to the pathophysiology of pyelonephritis. These findings may be relevant for the better understanding of host-parasite interactions and may be of clinical importance in the development of preventive intervention against P. aeruginosa induced pyelonephritis.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Mittal R,Sharma S,Chhibber S,Harjai Kdoi
10.1016/j.micpath.2008.08.003subject
Has Abstractpub_date
2008-11-01 00:00:00pages
323-30issue
5-6eissn
0882-4010issn
1096-1208pii
S0882-4010(08)00108-3journal_volume
45pub_type
杂志文章abstract::A total of 54 positive blood aerobic cultures of suspected blood sepsis in neonates were purified, diagnosed and tested for antibiotic susceptibility. Six bacterial pathogens of Escherichia coli (10 isolates), Klebsiella pneumoniae (10 isolates), Haemophilus influenzae (4 isolates), Pseudomonas aeruginosa (3 isolates)...
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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doi:10.1006/mpat.2000.0399
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
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更新日期:1994-12-01 00:00:00
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journal_title:Microbial pathogenesis
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doi:10.1016/j.micpath.2018.12.003
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abstract::Ehrlichia risticii, the causative agent of Potomac horse fever, is an obligate intracellular rickettsial organism. To understand the role of 55 and 51 kilodalton immunodominant antigens of E. risticii in strain variation, their genes from the 25-D and 90-12 strains were cloned, sequenced, and expressed in E. coli. Seq...
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
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abstract::Lung cancer, the leading cause of cancer mortality, needs urgent development of newly qualified diagnostic and therapeutic biomarkers. Recently, Human Endogenous Retroviruses (HERVs) have been introduced for cancer diagnosis. In this case-control study, we have collected blood samples from 60 lung cancer patients and ...
journal_title:Microbial pathogenesis
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abstract::The extracellular hemolysin (AHH1) gene of Aeromonas hydrophila ATCC7966 was cloned into Charomid9-28 in Escherichia coli DH1, and its complete nucleotide sequence determined. Escherichia coli carrying this gene expressed an extracellular heat-labile hemolysin for rabbit red blood cells. The minimum size of the coding...
journal_title:Microbial pathogenesis
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