当前位置： SCI文献检索 > MICROBIAL PATHOGENESIS期刊下所有文献 > Cloning and nucleotide sequence analysis of a Brucella abortus gene encoding an 18 kDa immunoreactive protein.

Cloning and nucleotide sequence analysis of a Brucella abortus gene encoding an 18 kDa immunoreactive protein.

Abstract：

:A DNA fragment encoding an approximately 18 kDa protein from Brucella abortus strain 2308 was cloned and expressed in Escherichia coli. This recombinant protein, designated BA18K, reacted in Western blot analysis with sera obtained from experimentally and naturally infected animals including mice, goats, dogs and humans. Restriction enzyme analysis of the plasmid (pBA28) encoding BA18K revealed the presence of an approximately 8.7 kbp Sau3A genomic DNA fragment within the vector and subsequent subcloning and Western blot analysis limited the region encoding BA18K to an approximately 3.0 kbp Pst 1 DNA fragment. DNA sequence analysis of this region identified an open reading frame capable of encoding a protein of 177 amino acids with a predicted relative molecular mass of 17529. Comparison of the deduced amino acid sequence of BA18K with those in the protein sequence databases yielded no homology with previously described proteins from other bacterial genera. These searches did, however, indicate that BA18K is identical to the previously described outer membrane protein (OMP) from B. abortus strain 544 designated Omp 19.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Kovach ME,Elzer PH,Robertson GT,Chirhart-Gilleland RL,Christensen MA,Peterson KM,Roop RM 2nd

doi

10.1006/mpat.1996.0108

subject

Has Abstract

pub_date

1997-04-01 00:00:00

pages

241-6

issue

4

eissn

0882-4010

issn

1096-1208

pii

S0882401096901084

journal_volume

22

pub_type

杂志文章

相关文献

MICROBIAL PATHOGENESIS文献大全
  • Serotyping of Brunei pneumococcal clinical strains and the investigation of their capability to adhere and invade a brain endothelium model.

    abstract：INTRODUCTION:Pneumococcal infections have caused morbidity and mortality globally. Streptococcus pneumoniae (pneumococci) are commensal bacteria that colonize the nasopharynx, asymptomatically. From there, pneumococci can spread in the lungs causing pneumonia and disseminate in the bloodstream causing bacteremia (sepsi...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.07.021

    authors： Rahman NA,Sharudin A,Diah S,Muharram SH

    更新日期：2017-09-01 00:00:00

  • Full-genome sequences of porcine circovirus 3 (PCV3) and high prevalence in mummified fetuses from commercial farms in Brazil.

    abstract：:The clinical implications of recently discovered porcine circovirus 3 (PCV3) infections are still unknown. The potential role of this emerging virus in reproductive loss in swine has been described. Herein, we report a high prevalence of PCV3 in mummified fetuses from sows maintained in modern farms in Rio Grande do S...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104027

    authors： Dal Santo AC,Cezario KC,Bennemann PE,Machado SA,Martins M

    更新日期：2020-04-01 00:00:00

  • Reduced virulence of a hfq mutant of Pseudomonas aeruginosa O1.

    abstract：:The Sm-like protein Hfq has been implicated in the regulation of sigmaS-dependent and sigmaS-independent genes in E. coli and in the regulation of virulence factors in both, Yersinia enterocolitica and Brucella abortus. Here, we have studied the effect of Hfq on virulence and stress response of Pseudomonas aeruginosa ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/s0882-4010(03)00149-9

    authors： Sonnleitner E,Hagens S,Rosenau F,Wilhelm S,Habel A,Jäger KE,Bläsi U

    更新日期：2003-11-01 00:00:00

  • Geniposide reduces Staphylococcus aureus internalization into bovine mammary epithelial cells by inhibiting NF-κB activation.

    abstract：:Staphylococcus aureus (S.aureus), a Gram-positive organism, is a frequent cause of subclinical mastitis. Geniposide, an iridoid glucoside isolated from the fruits of Gardenia jasminoides, has been reported to exhibit antibacterial and anti-inflammatory properties. The ability of S. aureus internalizing into bovine mam...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.10.002

    authors： Wei Z,Su K,Jiang P,Shi M,Wang J,Xie G

    更新日期：2018-12-01 00:00:00

  • Dendrimer-enabled transformation of Chlamydia trachomatis.

    abstract：:Lack of a system for genetic manipulation of Chlamydia trachomatis has been a key challenge to advancing understanding the molecular genetic basis of virulence for this bacterial pathogen. We developed a non-viral, dendrimer-enabled system for transformation of this organism and used it to characterize the effects of ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2013.08.003

    authors： Kannan RM,Gérard HC,Mishra MK,Mao G,Wang S,Hali M,Whittum-Hudson JA,Hudson AP

    更新日期：2013-12-01 00:00:00

  • Pros and cons of direct genotyping on tuberculosis clinical samples.

    abstract：OBJECTIVE:Prompt genotyping of Mycobacterium tuberculosis (M. tuberculosis) is crucial for improving molecular epidemiological investigation of tuberculosis (TB). METHODS:We performed a retrospective study to evaluate the use of 24 loci MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number of tandem-re...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2016.12.025

    authors： Sadegh H,Kargarpour Kamakoli M,Farmanfarmaei G,Masoumi M,Abdolrahimi F,Fateh A,Ebrahimzadeh N,Rahimi Jamnani F,Vaziri F,Siadat SD

    更新日期：2017-02-01 00:00:00

  • The role of IFN-gamma in murine Salmonella typhimurium infection.

    abstract：:Influence of both recombinant interferon gamma (r-IFN-gamma) and monoclonal anti-IFN-gamma on murine Salmonella typhimurium infection was studied in vivo. As a challenge we used either a virulent or an avirulent strain of S. typhimurium. The avirulent strain is unable to replicate in the mouse spleen. The effect of IF...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(90)90077-4

    authors： Muotiala A,Mäkelä PH

    更新日期：1990-02-01 00:00:00

  • Discrimination between apo and iron-loaded forms of transferrin by transferrin binding protein B and its N-terminal subfragment.

    abstract：:Many pathogens of the Pasteurellaceae and Neisseriaceae possess a surface receptor that binds transferrin (Tf) as an initial step in an iron acquisition process. This receptor is comprised of two proteins, transferrin binding protein A (TbpA) and transferrin binding protein B (TbpB). Since the ability to recognize the...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1998.0226

    authors： Retzer MD,Yu Rh,Zhang Y,Gonzalez GC,Schryvers AB

    更新日期：1998-10-01 00:00:00

  • In-silico designing, chemical synthesis, characterization and in-vitro assessment of antibacterial properties of some analogues of curcumin.

    abstract：:In the present work two key regulator proteins, monomeric MipZ of Caulobacter vibrioides (similar to Pseudomonas aeruginosa) and Pyruvate kinase of Staphylococcus aureus were docked with curcumin, the wonder molecule from the spice turmeric and structures of its twelve analogues were designed, synthesized and tested i...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.06.030

    authors： Shrivash MK,Mishra S,UpmaNarain,Pandey J,Misra K

    更新日期：2018-10-01 00:00:00

  • Characteristics of an Aeromonas trota strain isolated from cerebrospinal fluid.

    abstract：:Aeromonas are ubiquitous in aquatic habitats. However some species can cause infections in humans, but rarely meningitis. Here we describe the isolation and characterization of an Aeromonas strain from cerebrospinal fluid of a meningitis patient. The isolate, identified as A. trota by biochemical and molecular methods...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.01.017

    authors： Dallagassa CB,Surek M,Vizzotto BS,Prediger KC,Moriel B,Wolf S,Weiss V,Cruz LM,Assis FEA,Paludo KS,Rego FGM,Farah SMSS,Picheth G,Souza EM,Pedrosa FO,Chubatsu LS,Fadel-Picheth CMT

    更新日期：2018-03-01 00:00:00

  • Analysis of the influence of living environment and age on vaginal fungal microbiome in giant pandas (Ailuropoda melanoleuca) by high throughput sequencing.

    abstract：:A recent study has described the normal vaginal bacterial community in giant pandas, but there is a lack of knowledge of the fungal community residing in the vagina of giant pandas. In order to comprehensively understand the vaginal fungal microbial diversity and abundance in giant pandas, high throughput sequencing w...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.12.067

    authors： Chen D,Li C,Feng L,Zhang Z,Zhang H,Cheng G,Li D,Zhang G,Wang H,Chen Y,Feng M,Wang C,Wu H,Deng L,Ming H,Yang X

    更新日期：2018-02-01 00:00:00

  • Non-dystrophic 129 REJ mice are susceptible to i.p. infection with Listeria monocytogenes despite an ability to recruit inflammatory neutrophils to the peritoneal cavity.

    abstract：:In this study we compared the host response to Listeria monocytogenes in 129 REJ mice with listeria-resistant (C57Bl/6j) and susceptible (Balb/c) mouse strains. In all experiments mice were inoculated by the i.p. route. 129 REJ mice and Balb/c mice were sensitive to listeriosis whilst C57Bl/6j mice were relatively res...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1995.0032

    authors： Gahan CG,Collins JK

    更新日期：1995-05-01 00:00:00

  • Differential pathway network analysis used to identify key pathways associated with pediatric pneumonia.

    abstract：:We aimed to identify key pathways to further explore the molecular mechanism underlying pediatric pneumonia using differential pathway network which integrated protein-protein interactions (PPI) data and pathway information. PPI data and pathway information were obtained from STRING and Reactome database, respectively...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2016.10.023

    authors： Yang JB,Luo R,Yan Y,Chen Y

    更新日期：2016-12-01 00:00:00

  • Interaction of coagulase-negative Staphylococcus species with bovine mammary epithelial cells.

    abstract：:Three coagulase-negative Staphylococcus species (CNS) (Staphylococcus epidermidis, Staphylococcus xylosus and Staphylococcus hyicus), from the milk of cows with mastitis, were used to evaluate adherence to and internalization by bovine mammary epithelial cells, and to investigate involvement of host cell signal transd...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.2001.0465

    authors： Almeida RA,Oliver SP

    更新日期：2001-11-01 00:00:00

  • Protein profiles in mucosal and systemic compartments in response to Vibrio cholerae in a mouse pulmonary infection model.

    abstract：:We have recently shown that a mouse lung infection model resulting in acute pneumonia could be used for evaluating the protective immunity induced by mucosal vaccines against Vibrio cholerae. In order to gain insight and better understanding of the pathogenicity of V. cholerae infection, we identified and compared pro...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2015.07.002

    authors： Kang SS,Baik JE,Yang JS,Cho K,Yun CH,Han SH

    更新日期：2015-09-01 00:00:00

  • In vitro evidence of two-component system phosphorylation between the Mycobacterium tuberculosis TrcR/TrcS proteins.

    abstract：:Two-component regulatory proteins, histidine kinases and response regulators, function in bacteria as sensing and adaptive factors in response to a wide range of environmental stimuli. Conserved histidine and glycine regions of histidine kinase sensor proteins were used to design degenerate oligonucleotide primers for...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1998.0265

    authors： Haydel SE,Dunlap NE,Benjamin WH Jr

    更新日期：1999-04-01 00:00:00

  • Transcriptional regulation of Yersinia pestis biofilm formation.

    abstract：:Yersinia pestis, the causative agent of plague, is transmitted primarily by infected fleas in nature. Y. pestis can produce biofilms that block flea's proventriculus and promote flea-borne transmission. Transcriptional regulation of Y. pestis biofilm formation plays an important role in the response to complex changes...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,评审

    doi：10.1016/j.micpath.2019.04.011

    authors： Liu L,Zheng S

    更新日期：2019-06-01 00:00:00

  • The cold shock family gene cspD3 is involved in the pathogenicity of Ralstonia solanacearum CQPS-1 to tobacco.

    abstract：:Cold shock proteins (Csps) are small and highly conserved proteins that have target RNA- and DNA-binding activities. Csps play roles in different cellular processes and show functional redundancy. Ralstonia solanacearum, the agent of bacterial wilt, has 4 or 5 Csps based on genome analysis. However, the functions of a...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104091

    authors： Liu Y,Tan X,Cheng H,Gong J,Zhang Y,Wang D,Ding W

    更新日期：2020-02-20 00:00:00

  • Antioxidant defense response induced by Trichoderma viride against Aspergillus niger Van Tieghem causing collar rot in groundnut (Arachis hypogaea L.).

    abstract：:The study was conducted to examine the antioxidant enzymes induced by Trichoderma viride JAU60 as initial defense response during invasion of rot pathogen Aspergillus niger Van Tieghem in five groundnut varieties under pot culture. Seed treatment of T. viride JAU60 reduced 51-58% collar rot disease incidence in differ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2015.11.010

    authors： Gajera HP,Katakpara ZA,Patel SV,Golakiya BA

    更新日期：2016-02-01 00:00:00

  • Structural deformation in pathogenic bacteria cells caused by marine fungal metabolites: An in vitro investigation.

    abstract：:Over the past 50 years, fungal natural products have revolutionized medicine, yielding drugs which have enormous therapeutic potential. The aim of this study was to investigate the probable effect of marine fungal natural products on various skin pathogens. Initially, seventy natural extracts obtained from 35 differen...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104248

    authors： Agrawal S,Barrow CJ,Deshmukh SK

    更新日期：2020-09-01 00:00:00

  • Decrease of colonization in the chicks' cecum and internal organs of Salmonella enterica serovar Pullorum by deletion of cpdB by Red system.

    abstract：:Salmonella enterica serovar Pullorum (S. Pullorum) is a worldwide poultry pathogen of considerable economic importance, particularly in those countries with a developing poultry industry. A variety of genes that affect S. Pullorum colonization in chickens had been identified. 2',3'-cyclic phosphodiesterase (cpdB) is t...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2015.01.002

    authors： Liu H,Chen L,Wang X,Si W,Wang H,Wang C,Liu S,Li G

    更新日期：2015-03-01 00:00:00

  • Prevalence, molecular and antimicrobial resistance of Salmonella isolated from sausages in Meknes, Morocco.

    abstract：:Salmonella is among the most important food borne pathogens worldwide contaminating a wide range of animal products including meat products. The aims of this study go through two steps: The first step is to estimate the proportion of sausages products contaminated with Salmonella in Meknes city (Morocco), which were c...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.02.042

    authors： Ed-Dra A,Filali FR,Karraouan B,El Allaoui A,Aboulkacem A,Bouchrif B

    更新日期：2017-04-01 00:00:00

  • Acinetobacter nosocomialis secretes outer membrane vesicles that induce epithelial cell death and host inflammatory responses.

    abstract：:Acinetobacter nosocomialis is an important nosocomial pathogen that causes a variety of opportunistic infections; however, pathogenesis of this microorganism has not yet been characterized. The aim of this study was to investigate the secretion of outer membrane vesicles (OMVs) from A. nosocomialis and to determine th...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2015.03.012

    authors： Nho JS,Jun SH,Oh MH,Park TI,Choi CW,Kim SI,Choi CH,Lee JC

    更新日期：2015-04-01 00:00:00

  • Visfatin regulates the production of lipopolysaccharide-induced inflammatory cytokines through p38 signaling in murine macrophages.

    abstract：:Visfatin plays an important role in regulation of inflammatory cytokines. However, the role of visfatin under bacterial stress condition is not fully explored yet. Therefore, the present study was conducted for the better understanding of the regulation mechanism of visfatin on the production of inflammatory cytokines...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.02.002

    authors： Wu XT,Yang Z,Ansari AR,Xiao K,Pang XX,Luo Y,Song H

    更新日期：2018-04-01 00:00:00

  • First whole genome sequencing of Russian isolate of Capnocytophaga canimorsus, opportunistic pathogen causing lethal sepsis.

    abstract：:Capnocytophaga canimorsus is a part of healthy oral flora of dogs and cats. However, when it is transmitted to human subjects via animal bites or scratches, it can cause severe complications like endocarditis or even lethal septic shock, especially in immunocompromised persons. In this study, we performed the first wh...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.10.020

    authors： Shelenkov AA,Karan LS,Mihaylova YV,Yanushevich YG,Shipulin GA,Shagin DA

    更新日期：2018-12-01 00:00:00

  • Characterization of non-aflatoxigenic strains of Aspergillus flavus as potential biocontrol agent for the management of aflatoxin contamination in groundnut.

    abstract：:In the present study, nine non-aflatoxigenic Aspergillus flavus strains were evaluated for their potential to reduce A. flavus infection and aflatoxin contamination in groundnut. Genetic characterization of these strains revealed six different deletion patterns (A-F) for thirteen examined genes from the aflatoxin bios...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2016.11.007

    authors： Hulikunte Mallikarjunaiah N,Jayapala N,Puttaswamy H,Siddapura Ramachandrappa N

    更新日期：2017-01-01 00:00:00

  • Identification of oligopeptide-binding protein (OppA) and its role in the virulence of Streptococcus suis serotype 2.

    abstract：:The oligopeptide permease (Opp) cassette, an oligopeptide transport system belongs to the superfamily of ATP-binding cassette (ABC) transporter, is widely distributed in bacteria, including Streptococcus suis (S. suis). It is encoded by the opp operon containing oppA, oppB, oppC, oppD, and oppF. In addition to the upt...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.03.061

    authors： Zheng F,Shao ZQ,Hao X,Wu Q,Li C,Hou H,Hu D,Wang C,Pan X

    更新日期：2018-05-01 00:00:00

  • Alveolar microbiota profile in patients with human pulmonary tuberculosis and interstitial pneumonia.

    abstract：BACKGROUND:The presence of the human lung microbiota has been demonstrated in patients with different lung diseases, mainly in sputum samples. However, for study of the alveolar microbiota, a bronchoalveolar lavage (BAL) sample represents the lower respiratory tract (LRT) environment. It is currently unknown whether th...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.103851

    authors： Vázquez-Pérez JA,Carrillo CO,Iñiguez-García MA,Romero-Espinoza I,Márquez-García JE,Falcón LI,Torres M,Herrera MT

    更新日期：2020-02-01 00:00:00

  • Membrane proteins of Francisella tularensis LVS differ in ability to induce proliferation of lymphocytes from tularemia-vaccinated individuals.

    abstract：:T lymphocyte-mediated immunity is important for resistance to Francisella tularensis. To characterize the specificity of this immunity, we used membrane proteins and two lipopolysaccharide (LPS) preparations. Both membrane proteins were heat-modifiable, as indicated by their migration in sodium dodecyl sulphate polyac...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(89)90021-1

    authors： Surcel HM,Sarvas M,Helander IM,Herva E

    更新日期：1989-12-01 00:00:00

  • Molecular epidemiology of porcine reproductive and respiratory syndrome virus in Central China since 2014: The prevalence of NADC30-like PRRSVs.

    abstract：:Porcine reproductive and respiratory syndrome (PRRS), characterized by respiratory disorders in piglets and reproductive failure in sows, is still the great threat of swine industry. Recently, Emergence of the novel NADC30-like PRRS viruses (PRRSVs) has caused widespread outbreaks of PRRS. To investigate the epidemic ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.05.021

    authors： Wang LJ,Xie W,Chen XX,Qiao S,Zhao M,Gu Y,Zhao BL,Zhang G

    更新日期：2017-08-01 00:00:00