Abstract：
:In the present study, nine non-aflatoxigenic Aspergillus flavus strains were evaluated for their potential to reduce A. flavus infection and aflatoxin contamination in groundnut. Genetic characterization of these strains revealed six different deletion patterns (A-F) for thirteen examined genes from the aflatoxin biosynthesis pathway. Strain AFGS5 recorded maximum number of gene deletion (F) which included 12 out of 13 tested genes. Our findings indicated that aflR was the most frequently absent gene among the observed deletion patterns. A dendrogram inferred from combining random amplified polymorphic DNA and microsatellite data showed three of the non-aflatoxigenic strains segregating from other sampled isolates (aflatoxigenic and non-aflatoxigenic) tested. Greenhouse experiments, involving non-aflatoxigenic strains as biocontrol agents resulted in two strains, AFGS5 and AFGS12, which significantly reduced the population of aflatoxigenic fungi and the level of total aflatoxins in the rhizosphere/geocarposphere of soil samples as well as in groundnut seeds. Based on our findings, the use of these native non-aflatoxigenic strains; AFGS5 or AFGS12 in particular, as biopesticides could offer efficacious mitigation of aflatoxin contamination.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Hulikunte Mallikarjunaiah N,Jayapala N,Puttaswamy H,Siddapura Ramachandrappa Ndoi
10.1016/j.micpath.2016.11.007subject
Has Abstractpub_date
2017-01-01 00:00:00pages
21-28eissn
0882-4010issn
1096-1208pii
S0882-4010(16)30302-3journal_volume
102pub_type
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/j.micpath.2005.11.007
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doi：10.1016/j.micpath.2010.09.003
更新日期：2011-02-01 00:00:00
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更新日期：1994-05-01 00:00:00
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
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pub_type： 杂志文章
doi：10.1016/j.micpath.2019.103693
更新日期：2019-11-01 00:00:00
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journal_title：Microbial pathogenesis
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更新日期：2002-01-01 00:00:00
