Abstract：
:Group B streptococci (GBS) are opportunistic human pathogens that cause infection and invasive disease in newborns, pregnant women and non-pregnant adults. The internalization of GBS into eukaryotic cells occurs in an actin-microfilament dependent process. The objective of our study was to understand what host cell and/or bacterial factors may be involved in this process. We focused on alpha-actinin, an actin binding protein closely associated with cytoplasmic F-actin in the eukaryotic cell, to determine if it is involved in actin recruitment upon GBS internalization. Initial work revealed that GBS does not recruit alpha-actinin. However, it was found that alpha-actinin antibodies bound to the surface of the GBS, suggesting GBS possess surface-exposed actin binding protein(s). Slide agglutination experiments revealed that when the bacteria were emulsified with F-actin, visible agglutination occurred, further suggesting the presence of an actin binding protein on the GBS cell. Western blot analysis found that anti-alpha-actinin antibodies bound to a 42 kDa protein; mass spectra analysis identified this protein as GBS phosphoglycerate kinase (PGK). Competitive binding assays suggest that the PGK-actin interaction is not a factor in the initial binding of GBS to epithelial cells, however, treating epithelial cells with PGK prior to performing an invasion assay inhibited GBS internalization. This occurred in a dose dependent manner with 10 microg/mL of PGK inhibiting invasion by over 70%, and 50 microg/mL PGK inhibits GBS invasion completely.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Burnham CA,Shokoples SE,Tyrrell GJdoi
10.1016/j.micpath.2005.02.002subject
Has Abstractpub_date
2005-05-01 00:00:00pages
189-200issue
5-6eissn
0882-4010issn
1096-1208pii
S0882-4010(05)00019-7journal_volume
38pub_type
杂志文章abstract：:Orientia tsutsugamushi, an obligate intracellular bacterium, is the causative agent of scrub typhus which is histopathologically characterized by inflammatory manifestations, indicating that rickettsiae induce mechanisms that amplify the inflammatory response. To understand the pathogenesis of scrub typhus, we examine...
journal_title：Microbial pathogenesis
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pub_type： 杂志文章,meta分析
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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journal_title：Microbial pathogenesis
pub_type： 杂志文章,评审
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journal_title：Microbial pathogenesis
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abstract：UNLABELLED:The outcome of Helicobacter pylori infection has been related to specific virulence-associated bacterial genotypes. The vacuolating cytotoxin (vacA), cagA gene, oipA and babA2 gene are important virulence factor involving gastric diseases. The objective of this study was to assess the relationship between vi...
journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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更新日期：1991-04-01 00:00:00
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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更新日期：2019-08-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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更新日期：2006-07-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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更新日期：2017-04-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1999.0336
更新日期：2000-04-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(91)90045-c
更新日期：1991-09-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
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更新日期：2010-01-01 00:00:00
abstract：:The effect of recombinant Brugia malayi pepsin inhibitor (rBm33) on human monocytes/macrophages has been examined using THP-1 cells. THP-1 cells stimulated with rBm33 showed enhanced levels of expression of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6) and diminished levels of IL-12, iNOS and anti-inflammatory cytok...
journal_title：Microbial pathogenesis
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